- 1 - adenoexpress available with reporter genes: ad5.cmv-gfp ad5.cmv5-gfp ad5.cmv-lacz null...
TRANSCRIPT
![Page 1: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/1.jpg)
- 1 -
AdenoExpress™
Available withReporter genes:
Ad5.CMV-GFP
Ad5.CMV5-GFP
Ad5.CMV-LacZ
Null Vectors:
Ad5.Null
Ad5.CMV-Null
Wild Type Viruses:
Adenovirus Wild Type Serotype 5
Adenovirus Wild Type Serotype 2
![Page 2: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/2.jpg)
- 2 -
AdenoExpress™
Available with Biological Response Genes
CytokinesGM-CSF
IFN-
IFN-
IL-2
IL-12
![Page 3: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/3.jpg)
- 3 -
AdenoExpress™
Angiostatic genesAngiostatin
Endostatin
Tumor suppressorsP53
Co-StimulatoryB7.1
![Page 4: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/4.jpg)
- 4 -
AdenoExpress™
Format Concentration
(VP/mL)
Volume
(μL)
Total Qty
(VP)
Small 1X1011 100 1X1010
Large 1X1013 50 5X1011
Two Formats Available
![Page 5: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/5.jpg)
- 5 -
AdenoExpress™
Coronal Plane Section of Rat Striatums Injected with Ad5.CMV-GFP
Courtesy of Dr. Michael Y. Chen, NIH
![Page 6: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/6.jpg)
- 6 -
AdenoExpress™
Rat Spinal Cord Injected with Ad5.CMV-LacZ
Courtesy of Dr. Michael J. Iadarola, NIH
![Page 7: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/7.jpg)
- 7 -
AdenoExpress™
Mature Dentritic CellsInfected with Ad5.CMV-GFP
Courtesy of Dr. Peter Hevezi, EOS Biotech
![Page 8: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/8.jpg)
Visualizing gene expression by whole-body fluorescence imaging
Yang et al. (2000) Proc. Natl. Acad. Sci. USA 97, 12278-12282
AntiCancer Inc, San Diego, CA
![Page 9: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/9.jpg)
- 9 -
Ad5.CMV5-GFP
External and internal images of Ad5.CMV-GFP
![Page 10: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/10.jpg)
- 10 -
Ad5.CMV5-GFP
![Page 11: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/11.jpg)
- 11 -
Ad5.CMV5-GFP
External whole-body image of Ad5.CMV5-GFPin the brain 24h after gene delivery.
![Page 12: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/12.jpg)
- 12 -
Ad5.CMV5-GFP
External whole-body image of Ad5.CMV5-GFPgene expression in the liver
![Page 13: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/13.jpg)
- 13 - Cumate Inducible Expression System
![Page 14: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/14.jpg)
- 14 -
Cumate Inducible Promoter Regulatory Elements
![Page 15: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/15.jpg)
- 15 -
Cumate Inducible Promoter Strategy 2
![Page 16: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/16.jpg)
Applications of the Cumate Inducible
Promoter
![Page 17: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/17.jpg)
- 17 -
Generation of Recombinant Adenoviruses Expressing Toxic
Proteins
![Page 18: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/18.jpg)
- 18 -
Generation of Recombinant Adenoviruses Expressing Toxic
Proteins
![Page 19: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/19.jpg)
- 19 -
Inducible Expression in Stable Cell Lines
![Page 20: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/20.jpg)
- 20 -
Adenovirus-Based Expression Libraries
![Page 21: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/21.jpg)
- 21 -
Adenovirus-Based Expression Libraries
![Page 22: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/22.jpg)
- 22 -
Adenovirus Custom Services
![Page 23: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/23.jpg)
- 23 -
Adenovirus Custom Services
From cloning of your cDNA into an Adenovirus transfer
vector
to
cGMP Manufacturing
![Page 24: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/24.jpg)
- 24 -
Adenovirus Custom Services
Cloning of Gene into an Adenovirus Transfer Vector
Subcloning of your gene of interest into an adenovirus transfer vector.
Verification of the orientation of the subcloned insert by restriction mapping.
![Page 25: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/25.jpg)
- 25 -
Adenovirus Custom Services
Construction of a Recombinant Adenovirus
Production of a recombinant adenovirus by in vivo homologous recombination.
Plaque purification
Screening (Southern, Western, or by PCR).
![Page 26: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/26.jpg)
- 26 -
Adenovirus Custom Services
Plaque Purification of a Recombinant Adenovirus Prior to Amplification
Plaque purification assay and isolation of a pure recombinant adenovirus (when Ad provided by customer).
Identification of a pure recombinant adenovirus by Southern, Western or by PCR screening.
![Page 27: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/27.jpg)
- 27 -
Adenovirus Custom Services
Amplification of a Recombinant Adenovirus
Amplification of a recombinant adenovirus in 293 cells at a desired volume.
Infected cells are harvested and three freezing and thawing cycles are performed, followed by centrifugation to remove cell debris from recombinant adenovirus.
![Page 28: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/28.jpg)
- 28 -
Adenovirus Custom Services
Double Cesium Chloride Gradient Purification of a Recombinant Adenovirus
Amplified viral stocks should be purified in order to:
1. Remove all defective particles from the preparation,
2. Remove the condition media containing undesired contaminants as well as serum,
3. Resuspend the virus in a buffer more suitable for later use and long term storage,
4. Concentrate the virus to high levels.
![Page 29: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/29.jpg)
- 29 -
Adenovirus Custom Services
Double Cesium Chloride Gradient Purification of a Recombinant Adenovirus
Purification on a CsCl step gradient followed by a continuous CsCl gradient.
Dialysis of viral stock against desired buffer and aliquoting.
P.S. Purification of a virus is essential before undertaking any in vivo project.
![Page 30: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/30.jpg)
- 30 -
Adenovirus Custom Services
Concentration of a Purified Recombinant Adenovirus
Concentration of purified virus via a filter centrifugation up to 1013 VP/mL.
Viral particle titration (VP) by OD260
measurement.
![Page 31: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/31.jpg)
- 31 -
Adenovirus Custom Services
Identity Test of Viral Stock
Infection of non-permissive cells (ex. HeLa) with amplified viral stock.
Extraction of cellular proteins from infected cells.
Western blotting on extracted proteins.
![Page 32: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/32.jpg)
- 32 -
Adenovirus Custom Services
Recombinant Adenovirus Titration
1. Plaque Assay (Plaque Forming Units, PFU)
2. Tissue Culture Infectious Dose 50 (TCID50)
3. OD260 (Viral Particle, VP)
![Page 33: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/33.jpg)
- 33 -
Adenovirus Custom Services
Time Course and MOI Assay for Protein Production
Infection of HeLa cells using the recombinant adenovirus with three different MOIs.
Harvesting infected cells at two time points, typically at 48 and 72 hours.
![Page 34: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/34.jpg)
- 34 -
Adenovirus Custom Services
Large-Scale Protein Production in HeLa Cells
Infection of HeLa cells in serum-free media with the recombinant adenovirus.
Protein expression in HeLa cells, according to characteristics of the recombinant adenovirus or custom specifications.
Harvesting infected cells and concentration by centrifugation.
Delivery of a cell pellet and/or culture supernatant.
![Page 35: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/35.jpg)
- 35 -
Adenovirus Custom Services
Clinical Grade (cGMP) Production
Pilot project production of adenoviral vectors under GLP,
Adenoviral vector production, clinical (cGMP) grade,
Complete safety testing,
cGMP compliance and FDA registration.
![Page 36: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/36.jpg)
- 36 -
![Page 37: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/37.jpg)
- 37 - CONTRÔLE DE LA QUALITÉ
![Page 38: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/38.jpg)
- 38 -
Qu’est-ce que la qualité
Qu’est-ce que le contrôle de la qualité
Pourquoi le contrôle de la qualité?
Outils du contrôle de la qualité
Tâches de l’assistant QC
Qualités de l’assistant QC
Documentation et Assurance de la qualité
PlanPlan
![Page 39: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/39.jpg)
- 39 -
Qu’est-ce que la qualitéQu’est-ce que la qualité
Ensemble des propriétés et des caractéristiques d’un service ou d’un produit qui lui confèrent l’aptitude à satisfaire des besoins exprimés ou implicites
![Page 40: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/40.jpg)
- 40 -
Qu’est-ce que le contrôle de la qualitéQu’est-ce que le contrôle de la qualité
Vérification de la conformité du produit aux exigences spécifiées
![Page 41: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/41.jpg)
- 41 -
Pourquoi le contrôle de la qualité?Pourquoi le contrôle de la qualité?
Assurer une reproductibilité du produit
![Page 42: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/42.jpg)
- 42 -
Outils du contrôle de la qualitéOutils du contrôle de la qualité
Mode opératoire normalisé
Fiches analytiques
Assistant QC
![Page 43: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/43.jpg)
- 43 -
Tâches de l’assistant QCTâches de l’assistant QC
C on fo rm e
R ecom m en ce le tes t
H ors sp é c ifica tion s
V é rifica tion d es sp é c ifica tion s
L ec tu re d es ré su lta ts
E ffec tu e le tes t
H ora ire d es tes ts
R eço it u n é ch an tillon
![Page 44: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/44.jpg)
- 44 -
Qualités de l’assistant QCQualités de l’assistant QC
Autonome
Structuré
Polyvalent
Minutieux
Appliqué
Honnête
![Page 45: - 1 - AdenoExpress Available with Reporter genes: Ad5.CMV-GFP Ad5.CMV5-GFP Ad5.CMV-LacZ Null Vectors: Ad5.Null Ad5.CMV-Null Wild Type Viruses: Adenovirus](https://reader034.vdocuments.net/reader034/viewer/2022051614/551d9d7d497959293b8b46f7/html5/thumbnails/45.jpg)
- 45 -
Documentation et Assurance de la qualitéDocumentation et Assurance de la qualité
Vérification de la documentation pour s’assurer du respect des méthodes et des spécifications
Assurance de la qualité: ensemble des activités nécessaires pour donner une confiance adéquate que les produits satisferont aux exigences spécifiées