# 1226 clinical outcomes of achromobacter species in adult ... · (1984 – 2013) 306 cf patients...
TRANSCRIPT
(1984– 2013)306CFPatients
34(11%)Patients≥1sputum(+)withAchromobacter
24TransientColonizers 10(3.3%)Persistentcolonizers≥50%ofallculturesina1-yearperiod
(minimumof3cultures)≥1positivecultureandnotmeeting
criteriafor“persistent”
ClinicaloutcomesofAchromobacterspeciesinadultcysticfibrosispatients:Acohortstudy
Edwards BD,1 Somayaji R,1, Greysson-Wong J,2 Storey DG,3 Rabin HR,1,2 Surette MG,2,4.5 Parkins MD1,2
Background
• Achromobacter speciesareemergingpathogensincysticfibrosis(CF)withreportedprevalencerangingfrom5-29%
• Thereisapaucityofknowledgeregardingthepathogenicity,transmissioncharacteristics,andclinicalimpact ofthisorganism
Methods
• PatientsattendingtheSouthernAlbertaAdultCFclinicbetween1984– 2013with≥1sputumculturepositiveforAchromobacterspecieswereincluded
• Patientswerecategorizedintotransientandpersistentforanalysis(Figure1);thosewithpersistentinfectionwerealsomatched1:2withasexandage-matchedcontrolCFcohortforanalysis
• Datawascollectedthroughchartreviewtwoyearspre- andpost-initialAchromobacterinfection
• Achromobacterisolateswerecharacterizedwith16srRNA,PFGE,andnrdA genesequencing forspeciationanddetectionofclonality
• Outcomesofpulmonaryexacerbation(PEx)riskatinitialacquisitioncomparedwithpre- andpost-infection,lungfunctiondecline(FEV1%/year)andPExriskpre- andpost-infectionwereassessedwithregressionmodelsusingSTATA14.1(CollegeStn,TX)
• Atotalof34/306patients(11%)withamedianageof24.8yearswereidentifiedwithAchromobacterinfectioninthestudyperiod(Figure1)
• OfthosewithAchromobacterinfection,10/306(3.3%)developedpersistentinfectionandA.xylosoxidanswasthemostcommonspecies(50.0%)(Figure2)
• PatientsweremorelikelytoexperiencePExatinitialisolation(OR2.7;[95%CI1.2– 6.7];p=0.03)comparedwiththepriororsubsequentvisit
• BaselinecharacteristicsofpatientswithAchromobacterinfectionsandcontrolsweresimilar(Table1)
• Therewasnodifferenceinannuallungfunctiondecline(-1.08%[95%CI-2.73-0.57]vs.-2.74%[95%CI-4.02- -1.46];p=0.12)oroddsofPEx(OR1.21[95%CI0.45-3.28];p=0.70)followingpersistentAchromobacterinfection
• Twopatients(A182– apersistent colonizerandA184– atransientcolonizer)culturedAchromobacterxylosoxidans withmatchingPFGEandnrdA profiles(Figure3*)reflectingapossibletransmissionevent
• OtherinfectingspeciesidentifiedbynrdA sequencingwere A.insuavis(28%),A.dolens(11%),A.spanius(6%),andA.ruhlandii(6%)
• Therateoflungfunctiondeclinewasnotdifferentamongstspecies
UniversityofCalgary3330HospitalDriveNWCalgary,ABCANADA,[email protected]
#1226
• AchromobacterspecieshadaprevalenceandspeciesdistributionsimilartootherCFbasedstudiesinourcenter
• Achromobacter infectionwasassociatedwithPExatinitialacquisitionbutdidnothaveasignificantimpactonPExriskorrateofFEV1%declinefollowinginfection
• Largerstudiesarerequiredtoelucidatethetransmissionpotentialofthisorganism
Conclusions
DepartmentofMedicine1,DepartmentofMicrobiology,Immunology,andInfectiousDiseases2,DepartmentofBiologicalSciences3:UniversityofCalgary;DepartmentofMedicine4 andBiochemistry5,TheFarncombeFamily
DigestiveHealthResearchInstitute,McMasterUniversity
100806040
A329-Ax12-20/11/2013A329-Ax7-28/06/2011A274-Ax3-19/02/2005A312-Ax8-20/06/2012A312-Ax9-23/07/2012A128-Ax18-21/08/1996A128-Ax20-09/04/1997A061-Ax19-08/01/1997A061-Ax22-08/04/1999A061-Ax24-15/07/2000A085-Ax25-21/03/2001A085-Ax27-17/04/2002A349-AxN4-14/04/1997A358-Ax23-16/02/2000A022-Ax21-22/04/1998A077-Ax4-15/01/2009A077-Ax5-11/06/2009A182-Ax14-19/01/1994A182-Ax17-06/12/1994A184-Ax16-05/10/1994A182-Ax34-13/04/1993A217-Ax29-18/05/1988A217-Ax30-18/10/1989A061-Ax13-05/12/2013A253-AxN9-15/04/1993A335-Ax6-01/04/2010A222-AxN5-18/03/1998A222-AxN8-18/02/1992A222-AxN6-03/03/1999A222-AxN7-20/02/2002A222-AxN1-19/01/2005A222-AxN2-09/05/2007A222-AxN3-28/03/1994A207-Ax33-13/11/1991A207-Ax36-24/06/1991A207-Ax37-27/06/1991A003-Ax2-16/02/2005A090-Ax11-26/09/2012A039-Ax10-13/08/2013A152-Ax15-16/09/1994
}*
Aim
• Todeterminetheepidemiology,transmissibility,andclinicaloutcomesofAchromobacterspeciesinaNorthAmericanCFcohort
Table1:Baselinedataoftransient,persistentandcontrolcohorts
Baselinedata:2yearspriortoincidentAchromobacter culture*AzithromycinortobramycinattimeofAXcultureorControlstudyentry;✝Chronicco-infection;CFRD– CFrelateddiabetes;CFLD- CFliverdisease;DIOS– DistalIntestinalObstructionSyndrome
Figure1.Patientrecruitment
Figure3.DendogramofAchromobacterPFGE
Results
Figure2.Evolutionofchronicinfection
Total(n=34)
Transient(n=24)
Persistent(n=10)
Controls(n=18)
P-value(Transientvs.Persistent)
P-value(Persistentvs.
Control)Age,mean(SD) 26(9.3) 26(6.6) 27(14.3) 23(6.8) 0.8 0.45Male,no.(%) 15(44.1) 9(37.5) 6(60.0) 11(61.1) 0.23 0.95BMI(SD) 20(3.0) 20(2.8) 20(3.6) 21(2.7) 0.76 0.5
PancreaticInsufficiency,no.(%) 29(85.3) 20(83.3) 9(90.0) 13(72.2) 0.62 0.27FVC%,mean(SD) 77(26.8) 81(28.5) 70(21.6) 86(26.2) 0.25 0.11FEV1%, mean(SD) 58(27.1) 60(29.1) 55(22.6) 67(26.6) 0.63 0.22
*Inhaledtobramycin,no.(%) 7(20.6) 5(20.8) 2(20.0) 4(22.2) 0.96 0.89* Azithromycin,no.(%) 4(11.8) 3(12.5) 1(10.0) 4(22.2) 0.84 0.42InhaledCorticosteroid,no.(%) 8(23.5) 3(12.5) 5(50.0) 3(16.7) 0.02 0.06�P. aeruginosa,no.(%) 28(82.4) 20(83.3) 8(80.0) 12(66.6) 0.82 0.45�S. aureus,no.(%) 25(73.5) 17(70.8) 8(80.0) 7(38.8) 0.58 0.04HomeO2,no. (%) 4(11.8) 3(12.5) 1(10.0) 0(0.0) 0.84 0.17Co-Morbidities: - - - - - -CFRD,no.(%) 5(14.7) 3(12.5) 2(20.0) 3(16.7) 0.57 0.83SinusDisease,no.(%) 10(29.4) 6(25.0) 4(40.0) 10(55.6) 0.38 0.43BoneDisease,no.(%) 9(26.5) 7(29.2) 2(20.0) 4(22.2) 0.58 0.89CFLD,no.(%) 8(23.5) 6(25.0) 2(20.0) 2(11.1) 0.75 0.52DIOS,no.(%) 3(8.8) 3(12.5) 0(0.0) 2(11.1) 0.24 0.27
1985 1986 1987 1988 1989 1990 1991 1992 1993 1994 1995 1996 1997 1998 1999 /// 2007 2008 2009 2010 2011 2012 2013
Patient 6
Patient 9
Patient 12
Patient 18
Patient 21
Patient 23
Patient 24
Patient 25
Patient 26
Patient 33
A. xylosoxidans
A. xylosoxidans
A. xylosoxidans
= Death
= Lung Transplant
A. insuavis*
Unidentified species
A. xylosoxidans
A. insuavis
A. spanius
Unidentified species
* = Patient lost to follow-up (no sputum collected during this interim period)= Incidence of isolation of Achromobacter; Unless otherwise indicated, end of
boxes indicates spontaneous clearance.
A. xylosoxidans
= Period of clinic follow-up, including ongoing sputum culture = Moved to another center
Age: 25
Age: 16
Age: 63
Age: 20
Age: 28
Age: 15
Age: 24
Age: 23
Age: 39
Age: 19
Legend:Patient– Strain– Date(DD/MM/YYYY)