報告人 : 顏嘉宏指導教授 : 陳培展 老師

2009-10-06

The laminectomy was then performed at the T8–T9 level

SCI was induced by compressing the spinal cord for 1 min with an aneurysm clip calibrated to a closing pressure of 55 g.

The laminectomy was then performed at the T8–T9 level

SCI was induced by compressing the spinal cord for 1 min with an aneurysm clip calibrated to a closing pressure of 55 g.

SCI + premarin (1 mg/ml, iv.)SCI + saline (1ml/kg, iv.)

SCI + premarin (1 mg/ml, iv.)SCI + saline (1ml/kg, iv.)

spinal cord injury spinal cord injury

motor VEGF stain GDNF stain TUNEL stainTNF-, IL-10

motor VEGF stain GDNF stain TUNEL stainTNF-, IL-10

Animal: SD rat (300-400 gm) Anesthesia: phentobarbital (20mg/kg), I.P, ketamine (44 mg/kg)+ rompun (6.77 mg/kg) + atropine

(0.026 mg/kg), I.M

1,4,7days

SCI + premarinSham controls SCI + saline

The neuron apoptosis was detected by the terminal deoxynucleotidyl transferase(TdT)-mediated dUTP-fluorescence nick end labeling (TUNEL stain)

The TUNEL staining of spinal cord 7 days after SCI

SCI + premarinSham controls SCI + saline

X400 ☆ Primary Ab- Mouse monoclonal VEGF antibody [VG-1] ☆ Secondary Ab- Alexa-Fluor ® 568 goat anti-mouse (IgG) antibody

Numbers of the VEGF-positive cells in spinal cord section 7 days after SCI

SCI + premarinSham controls SCI + saline

X400 ☆ Primary Ab- Rabbit polyclonal to GDNF antibody

☆ Secondary Ab- FITC goat polyclonal anti-rabbit (IgG)

Numbers of the GDNF-positive cells in spinal cord section 7 days after SCI

Premarin increase endogenous GDNF and VEGF

7 days after SCI

Measurement of Serum TNF- and IL-10

SCI SD rat

PremarinPremarin

Improves Motor Performance

Reduce Spinal Cord Apoptosis

Premarin increase endogenous GDNF and VEGF

Premarin are beneficial in restoring the hindlimb function

TNF- (proinflammatory cytokine)

IL-10 (anti-inflammatory cytokine)

ConclusionsConclusions

感謝聆聽感謝聆聽問題與討論問題與討論