Template

2013.03. 14 XTBGXTBG1.

Illumina Miseq2.

3.

PCR4.

.

6.

Miseq

NightSHADE

Roche LightCycler 480II

THERMOFISHER Varioskan FlashCFlow

BIO-RAD ChemiDocXRS

PERCIVAL LED

Thermo Fisher PICO-21Tomy-SX500

Bionano

Thermo Fisher

Bio-RAD PCR

28

NightSHADE

XTBG8NightSHADE LB 985

CCDLEDluciferaseGFP9

tch4Plant Physiology vol 130 ,2002, p770-783TCH4, ,TCH4LUC11

121264813

Promoter Gene XLuciferase Genetranscript

XLuciferaseProtein XmRNA Luciferase

LUC

LUClucDNAATP16

CAB2 48hPaul F. Devlin: Signs of the time: environmental input to the circadian clock Journal of Experimental Botany, Vol.53,No.374.pp.1538-1550.July1,2002CAB2CAB217

Col- 03CCD4indiGOCCD Side mounting of camera

- -

--

Side mounting of camera

Side view of plant growing chambers

Plant Cell, Arabidopsis FHY3 Specifically Gates Phytochrome Signaling to Circadian Clock, 2006

Berthold FHY3(far-red elongated hypocotyl3)6CAB2-LUC1629Plant Cell, Arabidopsis FHY3 Specifically Gates Phytochrome Signaling toCircadian Clock, 200622

ELENA V. DOUKHANINA et.al , 2006

Expression of Human Nuclear Receptors in Plants for the Discovery of Plant-Derived Ligands25

Illumina Miseq System

XTBG27Illumina Miseqwww.themegallery.comCompany LogoIllumina Technology OverviewMiSeq IntroductionPerformanceApplications16S rDNAV(de-novo sequencing)Small RNA

Illumina Miseq

Illumina Miseq

135%201%300.1%Q20Q3020301G0.9G20Q2090%30 16s rRNAV(V3\V4),

1.16S rDNAV16s rRNAV(V3\V4)3116S rRNA Structure

Miseq 16SrRNA16S9Miseq16SV4254bp,bp32V4Liu Z, Lozupone C et.al 2007. Short pyrosequencing reads suffice for accurate microbial community analysis.

V4Miseq150bp250bpV446bpV4V4P5P724V433Phylum-level

MiseqHiseq2000read 1 Miseq34DNADNA N2Biorupter-UDC-200NexteraIllumina Miseq 16SrRNA V435

Bionano Qubit 2.0 Fluorometer

DNA A260=1.8-2.0DNA ug/ulinput DNADNA 300bp