Фотометр для микропланшет модель 680 & 680 xr (bio-rad) СИ.pdf

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  • Life Science Group

    Model 680 and 680 XR Microplate Reader

    Service Manual

    Part Number 100-2414 Revision E

  • 100-2414E 02/06 i

    PROPRIETARY NOTICE

    Information contained in this manual is copyrighted by Bio-Rad Laboratories, Inc. None of the contents may bereproduced in full or in part by any person without the prior written approval of Bio-Rad Laboratories, Inc. Thismanual is intended to provide information to assist the qualified field or instrument service technician to repair,maintain, and order replace parts for the instrument supplied. Every reasonable effort has been made to keepthe information in this manual current and accurate as of the date of publication or revision. However, no guar-antee is given or implied that the manual is error-free or accurate about any specification.

    Copyright 2002-2006 by Bio-Rad Laboratories, Incorporated. All rights reserved.

    Bio-Rad Laboratories Inc, Life Science Group

    2000 Alfred Nobel Drive

    Hercules, California 94547

    Telephone: 1-800-424-6723

  • ii 100-2414E 02/06

    About this manual This document is part of Life Science Groups commitment to provideyou, the service professional, with adequate information to effectivelysupport Bio-Rad instruments. Additional copies of this service documentmay be ordered using part number 100-2414.

    We welcome and appreciate any suggestions you may have to improveour service documentation. Please address your comments or sugges-tions to:

    Technical Editor, Global Service Support Dept.

    Bio-Rad Laboratories, Life Science Group

    2000 Alfred Nobel Drive

    Hercules, CA 94547

    FAX Number: 510 741-5802

  • 100-2414E 02/06 iii

    Revision Control List

    Document: Model 680 and 680 XR Microplate Reader Service ManualPart Number: 100-2414Date of Latest Revision: February 2006

    Each page of this manual has the catalog number, revision level and revision date for that page. Subsequentrevisions to a page will be indicated by changes to the revision level and revision date for that page. A sum-mary of changes for all revisions is listed in the following table.

    RevisionPage

    NumberDescription

    ChangedBy

    Date

    A - Initial Release A. Gandy Feb. 2002

    B 4-11 to 4-12 Updated Checkmark Plate procedure A. Gandy June 2002

    5-1 to 5-4 Added optics cleaning procedure

    6-1 to 6-25 Added part numbers to sections; Updated Figure 6-9

    6-16 Added check of plate position

    7-1 to 7-2 Added Checkmark Plate; Updated Figure 7-3

    7-4 Added Section AdjustingPlate Carrier Belt Tension

    7-6 Added Section Alignment of Plate Carrier

    8-5 Added Table 8-4 Checkmark Plate Failures

    C Sec 1 thru 10 Manual Updated to Add Model 680XR A. Gandy Mar 2004

    5-1 Added Note

    6-3 Added 6.3.3 Model 680 XR Lamp

    6-12 Added Table 6-1

    6-15 to 6-16 Added 6.3.11 Model 680 XR Lamp Control PCB

    6-22 Added 6.3.17 Model 680 XR Light Balancing Filter

    7-8 Added Table 2 Firmware History

    8-1 Updated Table 8-1

    9-3 to 9-11 Updated parts information

    App D Added Appendix D

    D 6-12 Added Backlit LCD to Table 6-1 A. Gandy Sept 2005

    7-6 Updated to reference Section 7.7

    7-9 Updated Firmware Table 7-2 and added 7.6.3

    7-10 to 7-11 Added Section 7.7 QC Software

    9-5 to 9-11 Updated parts

    E 7-8 to 7-9 Updated Firmware version 1.71; Table 7-2 update A. Gandy Feb 2006

    7-11 to 7-15 Updated 7.6.4 Firmware Downloading

    7-17 Added Section 7-8 External Printer Setup

    8-1 Updated troubleshooting printer

    9-11 Added Shield Cover

  • Table of Contents

    Section 1 Introduction 1.1 General Inforation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

    1.2 Service Philosophy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

    1.3 Safety and Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

    1.4 Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

    Section 2 System Overview 2.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1

    2.2 Product Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1

    2.3 Product Configurations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2

    2.4 Regulatory Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2

    2.5 Product Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

    2.5.1 Calculations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

    2.5.2 Limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

    2.5.3 Memory Backup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

    2.5.4 Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4

    2.5.5 RS-232 Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4

    2.6 Instrument Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

    2.6.1 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

    2.6.2 Optical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

    2.6.3 Performance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

    2.6.4 Thermal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6

    2.6.5 Electrical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6

    2.6.6 Physical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6

    Section 3 Installation 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

    3.2 Visual check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

    3.3 Installation Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

    3.4 Install Paper Roll (Optional Internal Printer) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

    3.5 Computer Interface (Optional). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3

    Section 4 System Checkout 4.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

    4.2 Tools and Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

    4.3 Summary of Tests and Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

    4.4 System Checkout Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

    4.4.1 General. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

    4.4.2 Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

    4.4.3 Preparing Yellow Dye Test Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

  • Table of Contents

    4.4.4 Startup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

    4.4.5 Touch Pad/Mixing Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

    4.4.6 Verify Printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4

    4.4.7 Start Microplate Manager. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4

    4.4.8 Air Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5

    4.4.9 Accuracy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7

    4.4.10 Channel-to-Channel Variation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8

    4.4.11 Stability and Drift . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9

    4.4.12 Verify Incubator Temperature (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-10

    4.5 Checkmark Validation Program (Optional Method) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-11

    4.5.1 General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-11

    4.5.2 Procedure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .