Фотометр для микропланшет модель 680 & 680 xr (bio-rad) СИ.pdf

Life Science Group

Model 680 and 680 XR Microplate Reader

Service Manual

Part Number 100-2414 Revision E

100-2414E 02/06 i

PROPRIETARY NOTICE

Information contained in this manual is copyrighted by Bio-Rad Laboratories, Inc. None of the contents may bereproduced in full or in part by any person without the prior written approval of Bio-Rad Laboratories, Inc. Thismanual is intended to provide information to assist the qualified field or instrument service technician to repair,maintain, and order replace parts for the instrument supplied. Every reasonable effort has been made to keepthe information in this manual current and accurate as of the date of publication or revision. However, no guar-antee is given or implied that the manual is error-free or accurate about any specification.

Copyright 2002-2006 by Bio-Rad Laboratories, Incorporated. All rights reserved.

Bio-Rad Laboratories Inc, Life Science Group

2000 Alfred Nobel Drive

Hercules, California 94547

Telephone: 1-800-424-6723

ii 100-2414E 02/06

About this manual This document is part of Life Science Groups commitment to provideyou, the service professional, with adequate information to effectivelysupport Bio-Rad instruments. Additional copies of this service documentmay be ordered using part number 100-2414.

We welcome and appreciate any suggestions you may have to improveour service documentation. Please address your comments or sugges-tions to:

Technical Editor, Global Service Support Dept.

Bio-Rad Laboratories, Life Science Group

2000 Alfred Nobel Drive

Hercules, CA 94547

FAX Number: 510 741-5802

100-2414E 02/06 iii

Revision Control List

Document: Model 680 and 680 XR Microplate Reader Service ManualPart Number: 100-2414Date of Latest Revision: February 2006

Each page of this manual has the catalog number, revision level and revision date for that page. Subsequentrevisions to a page will be indicated by changes to the revision level and revision date for that page. A sum-mary of changes for all revisions is listed in the following table.

RevisionPage

NumberDescription

ChangedBy

Date

A - Initial Release A. Gandy Feb. 2002

B 4-11 to 4-12 Updated Checkmark Plate procedure A. Gandy June 2002

5-1 to 5-4 Added optics cleaning procedure

6-1 to 6-25 Added part numbers to sections; Updated Figure 6-9

6-16 Added check of plate position

7-1 to 7-2 Added Checkmark Plate; Updated Figure 7-3

7-4 Added Section AdjustingPlate Carrier Belt Tension

7-6 Added Section Alignment of Plate Carrier

8-5 Added Table 8-4 Checkmark Plate Failures

C Sec 1 thru 10 Manual Updated to Add Model 680XR A. Gandy Mar 2004

5-1 Added Note

6-3 Added 6.3.3 Model 680 XR Lamp

6-12 Added Table 6-1

6-15 to 6-16 Added 6.3.11 Model 680 XR Lamp Control PCB

6-22 Added 6.3.17 Model 680 XR Light Balancing Filter

7-8 Added Table 2 Firmware History

8-1 Updated Table 8-1

9-3 to 9-11 Updated parts information

App D Added Appendix D

D 6-12 Added Backlit LCD to Table 6-1 A. Gandy Sept 2005

7-6 Updated to reference Section 7.7

7-9 Updated Firmware Table 7-2 and added 7.6.3

7-10 to 7-11 Added Section 7.7 QC Software

9-5 to 9-11 Updated parts

E 7-8 to 7-9 Updated Firmware version 1.71; Table 7-2 update A. Gandy Feb 2006

7-11 to 7-15 Updated 7.6.4 Firmware Downloading

7-17 Added Section 7-8 External Printer Setup

8-1 Updated troubleshooting printer

9-11 Added Shield Cover

Table of Contents

Section 1 Introduction 1.1 General Inforation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

1.2 Service Philosophy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

1.3 Safety and Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

1.4 Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

Section 2 System Overview 2.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1

2.2 Product Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1

2.3 Product Configurations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2

2.4 Regulatory Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2

2.5 Product Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

2.5.1 Calculations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

2.5.2 Limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

2.5.3 Memory Backup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

2.5.4 Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4

2.5.5 RS-232 Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4

2.6 Instrument Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

2.6.1 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

2.6.2 Optical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

2.6.3 Performance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

2.6.4 Thermal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6

2.6.5 Electrical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6

2.6.6 Physical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6

Section 3 Installation 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

3.2 Visual check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

3.3 Installation Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

3.4 Install Paper Roll (Optional Internal Printer) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

3.5 Computer Interface (Optional). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3

Section 4 System Checkout 4.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

4.2 Tools and Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

4.3 Summary of Tests and Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

4.4 System Checkout Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

4.4.1 General. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

4.4.2 Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

4.4.3 Preparing Yellow Dye Test Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

Table of Contents

4.4.4 Startup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

4.4.5 Touch Pad/Mixing Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

4.4.6 Verify Printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4

4.4.7 Start Microplate Manager. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4

4.4.8 Air Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5

4.4.9 Accuracy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7

4.4.10 Channel-to-Channel Variation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8

4.4.11 Stability and Drift . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9

4.4.12 Verify Incubator Temperature (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-10

4.5 Checkmark Validation Program (Optional Method) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-11

4.5.1 General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-11

4.5.2 Procedure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-11

Section 5 Preventative Maintenance5.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1

5.2 Tools and materials

5.3 Preventative Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1

5.3.1 Firmware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1

5.3.2 Inspection of Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1

5.3.3 Perform System Checkout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1

Section 6 Removal and Replacement 6.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1


6.3 Removal and Replacement Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1

6.3.1 Interference Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1

6.3.2 Model 680 Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1

6.3.3 Model 680 XR Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-3

6.3.4 Top Cover . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-4

6.3.5 Printer Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-5

6.3.6 Keypad Membrane. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-7

6.3.7 Inlet Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-8

6.3.8 Plate Carrier Assembly and Analog Board (#282) . . . . . . . . . . . . . . . . . . . . . . . . 6-9

6.3.9 CPU Board (#281) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-12

6.3.10 Incubator Control Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-13

6.3.11 Model 680 XR Lamp Control PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-15

6.3.12 Power Supply, 12 Volt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-17

6.3.13 Power Supply, 24 Volt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-18

6.3.14 Photo Sensor (Analog Board) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-19

Table of Contents

6.3.15 Photo Sensor (Filter Wheel) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-20

6.3.16 Model 680 Light Balancing Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-21

6.3.17 Model 680 XR Light Balancing Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-22

6.3.18 Plate Carrier Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-23

6.3.19 Optical Fiber Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-25

6.3.20 Filter Wheel/Filter Wheel Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-27

6.3.21 Fan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-28

6.3.22 Lithium Battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-29

Section 7 Adjustment and Calibration 7.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1


7.3 Electronics Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1

7.3.1 Setting of Jumper on the CPU Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1

7.3.2 Setting of Jumpers on the Printer Control Board . . . . . . . . . . . . . . . . . . . . . . . . . 7-2

7.3.3 Incubator Electronics Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-3

7.4 Adjusting Plate Carrier Belt Tension . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-4

7.5 Plate Carrier Alignment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-6

7.5.1 Checking of Plate Alignment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-6

7.5.2 Adjusting Plate Alignment - Y Direction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-7

7.5.3 Adjusting Plate Alignment - X Direction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-7

7.6 Firmware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-8

7.6.1 General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-8

7.6.2 Firmware History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-9

7.6.3 Integration of English and Chinese Firmware Version . . . . . . . . . . . . . . . . . . . . . 7-10

7.6.4 Downloading Firmware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-11

7.7 QC Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-16

7.7.1 Entering QC Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-16

7.7.2 Monitor Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-16

7.7.3 Plate Positioning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-17

7.8 External Printer Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-17

Section 8 Troubleshooting 8.1 About this Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1

8.2 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1

Table 8-1 General Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1

Table 8-2 Error Codes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-2

Table 8-3 Firmware Downloading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-4

Table of Contents

Section 9 Parts Information9.1 About this Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1

9.2 Parts Orders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1

9.2.1 US Domestic Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1

9.2.2 International Subsidiaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1

9.3 Returning Defective Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1

9.3.1 US Domestic Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1

9.3.2 International Subsidiaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-2

9.4 Service Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-3

Table 9-1 Replaceable Parts for the Model 680 Microplate Reader . . . . . . . . . . . . . . . . 9-3

Table 9-2 Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-11

9.5 Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-12

Table 9-3 Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-12

9.6 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-13

Table 9-4 Special Service Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-13

9.7 Manuals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-14

Table 9-5 Manuals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-14

Section 10 Drawings 10.1 About this Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-1

10.2 Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-1

Block Diagram Wiring Diagram

AppendixAppendix A Model 680 System Checkout Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-1

Appendix B Model 680 XR System Checkout Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-1

Appendix C Checkmark Check Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-1

Appendix D Error Flow Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D-1

Introduction

100-2414C 03/04 1-1

Section 1Introduction

1.1 General Information

This manual is the reference to be used for troubleshooting, repair, and maintenance of the Model 680 andModel 680 XR Microplate Readers. It is intended to be used by trained technicians and engineers familiar withsafety precautions relative to working with instrumentation. The procedures in this manual assume that thereader is familiar with the operation of the Model 680 and 680 XR Microplate Readers. Complete operatinginstructions are contained in the Model 680 and 680 XR Microplate Reader instruction manual. Informationpresented in the instruction manual is not repeated in this Service Manual unless there is a compelling reasonto do so.

1.2 Service Philosophy

This manual is designed to support mail in products. Regional service centers are the first level of service,where faulty assemblies can be most effectively repaired at a central repair facility. The second level of serviceis performed at our factory instrument service facility in Richmond, California, where additional service proce-dures requiring resources not available at regional service centers are performed.

1.3 Safety and Precautions

The Model 680 and 680 XR Microplate Reader contains the following Hazards:

Hazardous voltages

Thermally hot components

The following should be observed when servicing this unit:

When power is required, the unit should be plugged into an outlet with a properly grounded receptacle.The use of 2-prong plug adapters is not recommended and will void the safety certification of thisproduct.

The use of extension cords may compromise the safety of the operator and is not recommended.

Ensure that fuses installed in the unit are of the correct rating.

Ensure that none of the ventilation openings in the unit are blocked. Excessive heat buildup in the unitmay cause failures.

Bio-Rad is not responsible for any injury or damage caused by the use of this instrument for purposes otherthan for which it is intended or by modifications of the instrument not performed by Bio-Rad or an authorizedagent.

1.4 Conventions

The following conventions are used throughout this manual.:

Left and right sides of the unit are as viewed from the front, operators position, unless otherwise stated.

Dimensions are in millimeters, unless otherwise stated.

System Overview

100-2414C 03/04 2-1

Section 2System Overview

2.1 Introduction

The Model 680 and Model 680 XR Microplate Readers are an eight-channel, vertical pathlength photometerthat measures absorbance of the contents of 96 well mictotitration plates. They can perform single or dualwavelength measurements, and can report absorbance values to three decimal places.

The Model 680 and 680 XR Microplate Readers can be programed by entering commands through the keypadto define plate reading conditions and data analysis conditions as a protocol data, and select report types. Aprinted report is produced by an optional on-board thermal printer or external printer. An optional incubator isalso available.

The Model 680 and 680 XR Microplate Readers feature a built-in RS-232C serial interface device for conve-nient computer interfacing. The Microplate Manager software for personal computers offers a complete analy-sis program. Microplate Manager supports Windows 95, Windows NT and Windows 2000.

Microplate Manager Version 5.2.1 added support for the Model 680XR reader.

2.2 Product Description

Figure 1: Model 680/680 XR Microplate Reader

The following items are included with the Model 680/680 XR Microplate Reader system:

Microplate Reader with 415 nm, 450 nm, 490 nm and 655 nm interference filters installed

Power Cord

Spare Fuse

Dust Cover

Instruction Manual

Warranty Card

System Overview

2-2 100-2414C 03/04

2.3 Product Configurations

The Model 680 Microplate Reader is available in four configurations.

2.4 Regulatory Notices

This instrument is designed to meet Class A emissions and immunity levels of EN61326 product family stan-dard for CE mark. This rating requires that it only be used in a laboratory environment.

This instrument is designed and certified to meet EN61010 requirements which are internationally acceptedelectrical safety standards. Certified products are safe to use when operated in accordance with the InstructionManual. This instrument should not be modified or altered in any way. Alteration of this instrument will result invoiding of the manufacturers warranty, void regulatory certification and create a potential safety hazard.

This equipment has been tested and found to comply with the limits for Class A digital device, pursuant to Part15 of FCC rules. These limits are designed to provide a reasonable protection against harmful interferencewhen the equipment is operated in a commercial environment. This equipment generates, and can radiateradio frequency energy and if not installed and used in accordance with the Instruction Manual, may causeharmful interference to radio communications. Operation of equipment in a residential area is likely to causeharmful interference in which case the user will be required to correct the interference at their expense.

Table 1: Product Configurations

Catalog Number Description

168-1000 Model 680 Microplate Reader No Temperature Control and No Internal Printer

168-1001 Model 680 Microplate Reader With Temperature Control and No Internal Printer

168-1002 Model 680 Microplate Reader No Temperature Control and With Internal Printer

168-1003 Model 680 Microplate Reader With Temperature Control and With Internal Printer

168-1100 Model 680 XR Microplate Reader No Temperature Control and No Internal Printer

168-1101 Model 680 XR Microplate Reader With Temperature Control and With Internal Printer

System Overview

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2.5 Product Overview

2.5.1 Calculations

The Model 680 and 680 XR Microplate Readers uses Beers Law to calculate the absorbance value of each well. Beers Law states that absorbance is equal to the Log10 of the ratio on the baseline measurement intensity (Io) to the Sample measurement intensity (I).

Beers Law: Absorbance = Log10 (Io/I)

Before measuring the plate, the reader takes a reading for all eight photodiode channels. These values are recorded as the baseline measurement (Io) values for each channel, respectively. The reader then records the sample measurement (I) value for each well, and calculates the absorbance using these values. Chan-nel-to-channel error is significantly reduced because the Io value for a given channel is used only in deter-mining the absorbance of the wells of that channel.

2.5.2 Limits

The Model 680 and 680 XR Microplate Readers display absorbance readings with absolute values up to 3.500. Absolute values greater than 3.500 are out of range and are displayed as either *.*** or -*.***.

2.5.3 Memory Backup

The battery backup provides memory even after the reader is turned off. The following information will be saved in memory until new reading parameters are set by the user or a new plate is read by the instrument. Note: If reading a new plate is aborted before it is finished, all the previous plate reading date will remain in memory and none of the new data from the new plate is retained.

1. 10 latest plate reading data for End point protocol

2. 2 latest sets of Kinetic plate reading data (maximum 30 series of readings for each Kinetic)

3. End Point and kinetic protocol data

4. A set of Checkmark protocol data and the latest set of Checkmark read data

5. 8 wavelength values for filters

6. Laboratory name

7. 2 security passwords (Administrator and Common user)

The first time the instrument is turned on, or after a battery replacement, the following default information will hold in memory:

1. Both Administrator and Common user security password are set to 00000

2. Laboratory name is Bio-Rad Laboratories

3. All wavelength values of the filters are set to --- nm

4. The date and time of the calendar chip are set to 1 day, 1 month, 00 year, 0 hour and 0 minutes

5. None of the plate reading data is available

6. End Point protocol is chosen and the protocol number is set to #1

System Overview

2-4 100-2414C 03/04

2.5.4 Reports

Eight types of reports for End Point protocol can be generated by the Model 680 Microplate Reader:

1. Raw Data Report2. Absorbance report3. Limit Report4. Matrix Report5. Cut Off report6. Curve Fit Report7. Concentration Report8. Difference report

2.5.5 RS-232 Interface

The Model 680 Microplate Reader has a built-in RS232C interface (9 pin D-sub connector). This allows external computers to control the instrument. When an external computer is in control of the microplate reader, the printer and the keypad keys (except Start/Stop) are automatically deactivated and the display will indicate Remote Mode.

The reader will remain in the remote control mode until it receives RL command from the host computer or until the Start/Stop key is pressed.

RS-232 communication parameters are as follows:

Signals: TDX: Transmit DataRXD: Receive DataDTR: Data Terminal ReadyDSR: Data Set ReadyCTS: Clear to SendRTS: Ready to Send

Transmission: Baud Rate 9600, Data Width 8, Stop Width 1, Parity None

Data: ASCII (no distinction between upper and lower case)

Signal Assignment:

Host Computer9 Pin D-sub

Model 680 Reader9 Pin D-sub

Receive Data 2 ------------------------ 3 Transmit Data

Transmit Data 3 ------------------------ 2 Receive Data

Data Terminal Ready 4 ------------------------ 6 Data Set Ready

Signal Ground 5 ------------------------ 5 Signal Ground

Data Set Ready 6 ------------------------ 4 Data Terminal Ready

Request to Send 7 ------------------------ 8 Clear to Send

Clear to Send 8 ------------------------ 7 Request to Send

System Overview

100-2414C 03/04 2-5

2.6 Instrument Specifications

The Model 680 and 680 XR Microplate Reader specifications are as follows:

2.6.1 Features

Operating Panel: Membrane pad with 4 arrow keys, 7 function keys and 11 numeral keys

Display: LCD, 4 Lines x 20 Alphabet Characters

Computer Interface: Bi-directional RS-232C serial communication port, 9 pin D-sub receptacle

External Printer Port (only when internal printer option is not installed): Centronics interface with ASCIIcharacter code; 25 pin D-sub receptacle; 80 characters output per line

Report Types: Raw, absorbance, matrix, limit, cutoff, concentration, curve fit, difference, kinetic

Applicable Plates: Standard 96 well microtitre plate of polystyrene - flat, U, V rigid bottom

Mixing: 3 speeds (low, medium, high); Programmable time period 0-999 seconds

Warm-up Time: 3 minutes

2.6.2 Optical

Photometric Methods: Single and dual wavelength

Photo detectors: 8 silicon diodes for measurement and 1 silicon photodiode for reference

Light Source: Tungsten halogen lamp, 20 Watts with 3000 hours average lifetime

Model 680 Spectral Range: 400 nm to 750 nm

Model 680 XR Spectral Range: 340 nm to 800 nm

Interference Filters: Wavelength Tolerance +/- 3 nm; Band Width: 10 nm typical

Filter Capacity: 8 filters

Model 680 Standard Filters: 415, 450, 490, 655 nm

Model 680 XR Standard Filters: 340, 405, 415, 655 nm

Optional Filters: 340 through 800 nm (in 5 nm increments)

Fast Reading Speed: 6 seconds with single wavelength reading; 10 seconds with dual wavelengthreading

Standard Reading Speed: 15 seconds with single wavelength reading; 30 seconds with dual wave-length reading

Indication Range: 0.000 to 3.5 OD

2.6.3 Performance

Resolution: 0.001 OD

Accuracy: +/- 1.0% (or 0.010 from 0 to 3.0 OD at 490 nm)

Linearity: +/- 1.0% (from 0 to 2.0 OD); +/- 2.0% (from 2.0 to 3.0 OD)

Reproducibility: 1.0% (or 0.005 from 0 to 2.0 OD); 1.5% (from 2.0 to 3.0 OD)

Inter-Channel Variation: 1.5% (or 0.005 from 0 to 3.0 OD)

Stability and Drift: 0.010 OD at OD=1 with single wavelength reading

System Overview

2-6 100-2414C 03/04

2.6.4 Thermal

Incubator (Optional): Temperature Set Point: 25oC to 45oC (at least 5oC above ambient)

Set Point Accuracy: +/- 0.5oC (set point = 37oC and room temperature = 25oC)

Well-to-Well Uniformity: 0.5oC (set point = 37oC and room temperature = 25oC)

Warm-up Time: 5 minutes (set point = 37oC and room temperature = 25oC)

2.6.5 Electrical

Line Voltage: Universal Input: 100-120 VAC, 200-240 VAC, 50/60 Hz

Fuse: T3.15 Amp, 250 Volts, 20mm x 5mm

Power Consumption: 100 VA maximum

Battery: Lithium, CR2032, 3 Volts; average lifetime 5 years

2.6.6 Physical

Dimensions: 340 x 330 x 152 mm

Weight: 5.5 kg

Operating Environment: 5oC to 35oC, 0 to 95% RH, non condensing

Storage Environment: -20oC to 50oC, 0 to 95% RH, non condensing

Installation

100-2414C 03/04 3-1

Section 3Installation

3.1 Introduction

The Model 680 and 680 XR Microplate Readers are easy to install. Prior to installation, verify that all systemcomponents have been received.

3.2 Visual Check

1. Check this instrument for any signs of damage which may have occurred during shipping.

2. Check the filters: Open the rear cover by pushing the release latch on the top. Check that the filters are installed into the proper positions per Table 1:

Table 1 - Filter Installation

3. Check to insure filters are properly seated (Figure 1).

Figure 1: Filter Check

Filter Position Model 680 Model 680XR

1 415 nm 340 nm

2 450 nm 405 nm

3 490 nm 415 nm

4 655 nm 655 nm

5 User User

6 User User

7 User User

8 User User

Installation

3-2 100-2414C 03/04

3.3 Installation Procedure

1. Place the instrument on a clean, sturdy table or bench. It is important to keep the instrument in a clean, relatively dust free environment to ensure maximum performance.

2. Connect the power cord to the connection on the back if the instrument.

3. Turn ON the power switch on the rear panel. The LCD will display the version number of the on board firmware. After about three seconds, the instrument will perform a self test diagnosis that takes about fifteen seconds.

4. When prompted by the Log In screen, enter the password (password = 00000). Press Enter.

5. Allow three minutes for the instrument to warm up prior to reading plates.

3.4 Install Paper Roll (Optional Internal Printer)

1. Open the rear cover by pushing the release latch on the top.

2. Tear off a small piece of paper to form a diagonal point on the end of the paper roll.

3. Place the paper roll in the holder positioned below the printer and orient the roll so that it will feed from the bottom (Figure 2).

Figure 2: Orientation of Paper Roll

4. While pressing the Paper Feed key, direct the pointed end of the paper into the paper feed slot in the underside of the printer until the printer grabs the paper and feeds it through the slot in the top of the instrument.

Installation

100-2414C 03/04 3-3

3.5 Computer Interface (Optional)

1. Some customers may utilize the system with a computer and microplate software such as Microplate Manager (or Checkmark).

2. Set up computer as required and install software.

3. Install serial cable from computer to Model 680 oe 680 XR.

4. Turn on instrument, enter password.

5. Verify communication with instrument using Microplate Manager.

Note: Microplate Manager version 5.2.1 is required for Model 680XR support.

System Checkout

100-2414C 03/04 4-1

Section 4System Checkout

4.1 Introduction

This section provides the system checkout procedure for the Model 680 and 680 XR Microplate Reader. Suc-cessful completion of the tests in this section represent the Operational Qualification of the instrument. A Sys-tem Checkout Report is available in Appendix A and B.

The system checkout procedures for the Model 680 and 680 XR Microplate Readers require the use of aremote computer using Microplate Manager software.

The Model 680 and 680 XR Microplate Reader contains an on board checkout procedure, Checkmark Valida-tion Program, which requires the use of a special Checkmark test plate.

4.2 Tools and Materials

Microplate Manager Software, 170-9520

RS-232 Cable, 931-0184

Printer

Digital Temperature Multi-meter with 0.1 mm diameter bead type K thermoprobe (minimum +/- 0.5 C temperature meter accuracy)

96 Well Microtitre Plate, Flat Bottom, 224-0096

Micro Pipette, 50L

Pipette Tips

50 ml beaker

Acid Yellow 99, Fisher AC19178-100

Distilled Water

Tween 20, 170-6531

Checkmark Reader Performance Check Plate, 170-6940 (Optional)

System Checkout

4-2 100-2414C 03/04

4.3 Summary of Tests and Specifications

The following is a summary of the tests and specifications for the Model 680 and 680 XR Microplate Readers.

Table 1: Summary of Tests and Specifications

Test Parameter Specification

Firmware Firmware Version Record Version

Instrument Function Keypad and Display Proper Function

Internal Printer (optional feature) Proper Function

Startup and Self Diagnosis Proper Function

RS-232 Communication Proper Communication

Mixing Feature Proper Function

Incubator Set Point 37 C (optional feature) 37 C +/- 0.5 C

System Checkout Air Read < 0.005 OD

Accuracy CV < 5%

Channel-to-Channel CV < 5%

Stability and Drift < 0.010 OD

System Checkout

100-2414C 03/04 4-3

4.4 System Checkout Procedure

4.4.1 General

1. All system checkout procedures in this section will be performed with Microplate Manager via PC computer.

2. The Checkmark Validation Program, if available, may be used for system checkout as an alternate method (paragraph 4.5).

4.4.2 Setup

1. Setup computer.

2. Install Microplate Manager.

3. Install HPK protection key.

4. Connect RS-232 cable from computer to reader.

4.4.3 Preparing Yellow Dye Test Plate

1. System checkout requires the preparation of a yellow dye pipetted into a microtitre plate. Pipetting must be of extreme accuracy as the Model 680 reader is sensitive enough to detect pipetting errors.

2. Prepare the yellow dye as follows:

Mix: 2 mg Acid Yellow 9950 ml Distilled Water0.01 ml Tween 20

3. Pipette 200 microliters yellow dye into each well of the microtitre plate (96 total).

4. The value of the prepared test plate is 0.424 - 0.444 OD.

4.4.4 Startup

1. Turn ON the reader power switch. The LCD will display the version number of the on board firmware. Record Firmware Version.

2. After about three seconds, the instrument will perform a self test diagnosis that takes about ten sec-onds.

3. When prompted by the Log In screen (Administrator), enter the password 00000. Press Enter.

4.4.5 Touch Pad/Mixing Function

1. Open chamber door (leave open for this test).

2. Press Memory Recall button.

3. Select Protocol and press Enter.

4. Select End Point and press Enter.

5. Select End Point 01 and press Enter twice.

6. Press the Start button.

7. Observe that plate carrier vibrates for 3 seconds prior to read.

System Checkout

4-4 100-2414C 03/04

4.4.6 Verify Printer (Optional Feature)

1. After completion of the above End Point 01 test, press the Print button.

2. Select Reports and press Enter.

3. Verify the printer is functioning properly.

4.4.7 Start Microplate Manager

1. Start Microplate Manager.

2. Select File...Select Reader. Select Model 680 or Model 680 XR and click OK.

3. Select New Endpoint Protocol. The Microplate Manager window will appear (Figure 1).

Figure 1: Microplate Manager - Endpoint Protocol

4. The instrument will be placed in remote mode. Remote Mode will appear on display and touch keys will become nonfunctional.

System Checkout

100-2414C 03/04 4-5

4.4.8 Air Read

1. This test is performed without the use of a microtiter plate.

2. Press Show Template button.

3. Click the Turn Off Autofill Icon.

4. Click the Format as Unknowns Icon.

5. Using the curser, click and drag highlighting all rows (A1 to G12).

6. All wells on the template should be squares filled with 1s (see Figure 2).

Figure 2: Plate Template

7. Minimize the plate template.

8. Click on Reports.

9. Check the Raw Data Report. Click OK. (See Figure 3).

Figure 3: Raw Data Report Setup

System Checkout

4-6 100-2414C 03/04

10. Check the Reading Parameters Single box.

11. Select Measurement filter: 490.

12. Click the Run button.

13. Plate will take a few moments to read and display the Raw Data Report.

14. Ensure that all values are < 0.005. Reference Figure 4.

Figure 4: Raw Data Report

System Checkout

100-2414C 03/04 4-7

4.4.9 Accuracy

1. This test is performed with the use of the yellow dye test plate.

2. Use the formatted plate template from the Air Read above.

3. Click on Reports.

4. Check the Raw Data Report and Unknown Concentrations. Click OK. See Figure 5.

Figure 5: Report Selection

5. Check the Reading Parameters Single box.

6. Select Measurement filter: 490.

7. For Mix Time (sec) enter 3.


9. Ensure the CV is < 5% on the Unknown Concentrations Report (Figure 6) and target values are within expected ranges (Mean OD).

Figure 6: Unknown Concentrations Results

System Checkout

4-8 100-2414C 03/04

4.4.10 Channel-to-Channel Variation

1. This test is performed with the use of the yellow dye test plate.

2. Press Show Template button.

3. Click the Turn Off Autofill Icon.

4. Click the Format as Unknowns Icon.

5. Using the curser, click and drag highlighting rows A1 to A12. The number 1 should appear in each box. Repeat highlighting rows B1 to B12. The number 2 should appear in each box. Repeat for the remaining rows C1 to C12, D1 to D 12, etc. All wells on the template should appear as shown in Fig-ure 7.

Figure 7: Channel to Channel Setup

6. Minimize the plate template.

7. Use the same format for reports (Raw Data Report and Unknown Concentrations), filter (490) and mix time (3).


9. Plate will take a few moments to read and display the Raw Data Report and Unknown Concentra-tions Report.

10. Ensure the CVs are < 5% on the Unknown Concentrations Report (Figure 8) and target values are within expected ranges (Mean OD).

System Checkout

100-2414C 03/04 4-9

Figure 8: Unknown Concentrations Report

4.4.11 Stability and Drift

1. This test uses the same setup as specified in 4.4.11 Channel-to-Channel Variation above.

2. Rerun the Channel-to-Channel variation test 3 times allowing 10 minutes between tests.

3. Compare the data from the three tests. The average mean from each row shall be within 0.010 OD of each other.

System Checkout

4-10 100-2414C 03/04

4.4.12 Verify Incubator Temperature (Optional Feature)

1. Place checkmark in Incubator On box. Insure set point is 37 C.

2. Place a standard 96 well microtitre plate filled with 200 microliters water in wells A1, A6, A12, D6, D9, H1, H6, and H12 in the plate carrier. See Figure 9.

Figure 9: Designated Temperature Test Points

3. Allow microtiter plate to warm for 30 minutes.

4. After 30 minutes, immerse the thermoprobe in the designated well filled with the 200 microliters of water without touching any portion of the inner well wall. The immersed probe must be in the middle of the well and immersed in water. See Figure 10.

Figure 10: Well Temperature Measurement

5. Verify that the actual well temperature is 37 C +/- 0.5 C for each well (A1, A6, A12, D6, D9, H1, H6, and H12).

System Checkout

100-2414C 03/04 4-11

4.5 Checkmark Validation Program (Optional Method)

4.5.1 General

Figure 11: Checkmark Check Plate

1. The Checkmark program is used with the Checkmark Reader Performance Check Plate for validat-ing the performance of the Model 680 Microplate Reader. It will test the following parameters:

Default Measurements

Accuracy

Precision

Linearity

Optional Measurements

Plate Positioning

Cross-talk

Filter Check

0 OD and Overflow

IR Spectral Blocking

2. The Checkmark program resides in the Model 680 Microplate Reader programs.

4.5.2 Procedure

Entering Checkmark Plate Reference Data

1. Turn on Model 680 Reader and enter password.



4. Select Checkmark and press Enter. Press any key if instructed.

5. Press the Edit button.


System Checkout

4-12 100-2414C 03/04

7. Select Serial/Report and press Enter

8. Enter serial number of Checkmark plate. Select report type Simple or Full. Press Enter.

9. Select Linearity and press Enter.

10. Enter appropriate data from Checkmark reference plate data. Press enter when complete.

11. Select Max CV and press Enter.


13. Select Filter Check Reference and press Enter.


Running the Test

1. Open chamber door, insert Checkmark Reader Performance Check Plate, and close door.



4. Select Checkmark and press Enter. Press any key if instructed.

5. Press the Start button.

6. Test will take about 3 minutes to read plate.

Preventative Maintenance

100-2414C 03/04 5-1

Section 5Preventative Maintenance

5.1 Introduction

This section provides the preventative maintenance procedure for the Model 680 and 680 XR MicroplateReader. Preventative maintenance is recommended at least once a year.


Small Hand Tools

Cleaning Materials (Lint Free Cloth, Lens Tissue, Isopropyl Alcohol)

5.3 Preventative Maintenance

5.3.1 Firmware

1. Connect the power cord to the connection on the back if the instrument.

2. Turn ON the power switch on the rear panel. The LCD will display the version number of the firm-ware.

3. Record firmware version.

4. Turn OFF instrument.

5. Update to latest firmware if applicable (see Section 7.6).

5.3.2 Inspection of Instrument

1. Remove top cover.

2. Visually inspect for cleanliness of plate carrier. Clean plate carrier and rails with lint free cloth and isopropyl alcohol.

Note: Do not use grease on the plate carrier or rails.

3. Visually inspect for cleanliness of lenses. Clean as required with lens tissue and isopropyl alcohol (see Section 5.3.3).

4. Reassemble instrument.


5-2 100-2414C 03/04

5.3.3 Optics Cleaning


2. To access top lenses, remove the two screws securing the fiber optics to the cover (Figure 5-1).

Figure 5-1: Remove Screws Securing Fiber Optics

3. Remove two hex standoffs securing top lens block.

4. To remove lenses, remove ten securing screws (Figure 5-2).

Figure 5-2: Top Lens Block

5. Clean lenses with lint free cloth and isopropyl alcohol.

6. Reassemble lenses into holder and remount.

Note: Check for proper orientation when remounting.


100-2414C 03/04 5-3

7. To access bottom lenses, remove cover (Figure 5-3).

Figure 5-3: Bottom Lenses

8. Clean lenses as required. If removal of bottom lenses is required for cleaning, remove plate carrier and analog board (see Section 6.3.12).

9. Remove bottom lens block (Figure 5-4).

Figure 5-4: Bottom Lens Block Screws


5-4 100-2414C 03/04

10. Remove lenses from holder to clean (Figure 5-5).

Figure 5-5: Bottom Lens Holder

11. Clean with lint free cloth and isopropyl alcohol.

12. Reassemble instrument. Note proper orientation of bottom lens holder upon resaaembly.

5.3.4 Perform System Checkout

1. Perform system checkout per Section 4.0 of this Service Manual.

Removal and Replacement

100-2414D 09/05 6-1

Section 6Removal and Replacement

6.1 Introduction

This section provides the parts removal and replacement procedures for the Model 680 and 680 XR MicroplateReader.


Standard Hand Tools

Voltmeter

6.3 Removal and Replacement Procedures

6.3.1 Interference Filters


2. Rotate the filter wheel until the desired filter is in top position. Grasp top of filter frame to and pull up to remove filter.

3. To install filter, insert into desired slot until filter locks into place.

6.3.2 Model 680 Lamp (1681006)


Caution: Surface of Cover may be Hot!

2. Remove the two screws that hold the black plastic cover in place (Figure 6-1) and remove cover.

Figure 6-1: Screws Securing Cover


6-2 100-2414D 09/05

3. Remove the screw which holds the metal cover in place (Figure 6-2) and remove cover.

Figure 6-2: Screw Securing Metal Cover (Caution: Hot)

4. Carefully pull the lamp free and remove (Figure 6-3).

Figure 6-3: Lamp Removal

5. Install new lamp in reverse order of the above steps.


100-2414D 09/05 6-3

6.3.3 Model 680 XR Lamp (1681104)


Note: Surface of Cover may be Hot! Allow to cool before replacing lamp.

2. Remove the two screws (arrows) that hold the black plastic cover in place (Figure 6-4) and remove cover assembly.

Figure 6-4: Screws Securing Cover

3. Remove the lamp (Figure 6-5).

Figure 6-5: Lamp Removal

4. Install new lamp.

Note: Do not tough new lamp with fingers. Oil on lamp may reduce lamp life.

5. Reassemble in reverse order of the above steps.


6-4 100-2414D 09/05

6.3.4 Top Cover (9310161)

1. Remove the six screws from the underside of the instrument (Figure 6-6).

Figure 6-6: Remove Six Screws from Underside of Instrument

2. Remove the top cover to left side of the instrument (Figure 6-7).

Figure 6-7: Remove Top Cover to Left Side of Instrument

3. Install cover in reverse order of the above steps.


100-2414D 09/05 6-5

6.3.5 Printer Assembly (9310160)


2. Cut the cable tie securing the cable and remove cable from the circuit board (Figure 6-8).

Figure 6-8: Cut Cable Tie and Remove Cable

3. Remove three screws securing printer assembly and remove assembly (Figure 6-9).

Figure 6-9: Three Screws Securing Printer Assembly

4. Prior to installing printer assembly, set the jumpers on the printer control board as follows:


6-6 100-2414D 09/05

5. Check printer head type on flexible cable (Figure 6-10).

Figure 6-10: Location of Head Type

6. Configure the JP4 jumpers in accordance with the Figure 6-11.

Figure 6-11: JP4 Jumper Settings

7. Install new printer in reverse order of the above steps.


100-2414D 09/05 6-7

6.3.6 Keypad Membrane (9310177, 9310277, 9310229)


2. Cut cable tie and disconnect three cables from keypad board (Figure 6-12).

Figure 6-12: Keypad Board

3. Remove three screws and lift out keypad board (Figure 6-13).

Figure 6-13: Screws Securing Keypad Board

4. Peel off keypad membrane (Figure 6-14).

Figure 6-14: Peel Off Keypad Membrane

5. Install new keypad membrane in reverse order of the above steps.


6-8 100-2414D 09/05

6.3.7 Inlet Assembly (9310167)


2. Disconnect the terminals from board (Figure 6-15).

Figure 6-15: Disconnect Terminals

3. Remove nuts from interface connectors and remove (Figure 6-16).

Figure 6-16: Remove Nuts from Connectors

4. Remove four screws securing the fan (Figure 6-17).

Figure 6-17: Fan Mounting Screws

5. Remove inlet assembly.

6. Install new inlet assembly in reverse order of the above steps.


100-2414D 09/05 6-9

6.3.8 Plate Carrier Assembly and Analog Board

Note: Check the model and serial number for the correct replacement board.

Table 6-1: Analog Board Part Numbers


2. Loosen the two screws which secure the fiber and pull out fiber (Figure 6-18).

Figure 6-18: Fiber Mounting

3. Remove the nine mounting screws (Figure 6-19).

Figure 6-19: Plate Carrier Mounting Screws

Model 680 Model 680 XR

Analog Board 9310182 9310289


6-10 100-2414D 09/05

4. Lift the plate carrier assembly, disconnect the cables and remove assembly (Figure 6-20).

Figure 6-20: Disconnect Cables

5. Remove two screws securing the cover over the analog board (Figure 6-21).

Figure 6-21: Analog Board Mounted to Plate Carrier


100-2414D 09/05 6-11

6. Carefully remove fiber from mounting clips (Figure 6-22).

Figure 6-22: Clips Securing Fiber

7. Remove four screws securing analog board and remove (Figure 6-23).

Figure 6-23: Analog Board Mounting Screws

8. Install new plate carrier assembly or analog board in reverse order of the above steps.


6-12 100-2414D 09/05

6.3.9 CPU Board

Note: Check the model and serial number for the correct replacement board.

Table 6-1: CPU Board Part Numbers

1. Open top cover.

2. Remove plate carrier assembly (see 6.3.8).

3. Remove all cables to CPU board.

4. Remove four mounting screws and remove board (Figure 6-24).

Figure 6-24: CPU Board Mounting

5. Install new CPU board in reverse order of the above steps.

6. Set the jumper on the CPU Board (#281) per the following table:

Model 680Old LCD

Model 680New LCD

Model 680Backlit LCD

Model 680 XR

Serial Number 10001 to 10735 10736 to 13850 13851 and higher All

CPU Board 9310181 9310225 9310353 9310278

Table 6-2: JP2 Jumper Settings for CPU Board

Instrument Configuration

Jumper Jumper Placement

Without Incubator JP2 Jump

With Incubator JP2 No Jumper (Open)


100-2414D 09/05 6-13

6.3.10 Incubator Control Board (9310180)

1. Open top cover.


3. Remove all cables to incubator control board.

4. Remove four mounting screws and remove board (Figure 6-25).

Figure 6-25: Incubator Control Board Mounting

5. Install new incubator control board in reverse order of the above steps.

6. Perform incubator electronics adjustment as follows. Disconnect cable from plate carrier to CN52.

7. Connect cable into Incubation Adjustment Tool, 9310183, 107.79 ohm connector on tool.

8. Turn on instrument.

9. Measure voltage between TP5 and TP0 (see Figure 6-26).


6-14 100-2414D 09/05

Figure 6-26: Incubator Board Adjustments

10. Adjust trim pot VR1 until the voltage measures 0.000V +/- 0.001V.

11. Connect cable into Incubation Adjustment Tool, 116.47 ohm connector on tool.

12. Measure voltage between TP5 and TP0.


14. If voltage is out of range, repeat steps 3 through 9.


100-2414D 09/05 6-15

6.3.11 Model 680 XR Lamp Control PCB (9310276)

1. Open top cover.

2. Remove ground screw connection and four mounting screws and lift up the filter wheel/lamp assem-bly (Figure 6-27).

Figure 6-27: Lamp Control PCB is Located Under Lamp Assy

3. For ease of disassembly, turn over assembly. To access lamp control board, remove the five screws securing the lamp PCB cover (Figure 6-28).

Figure 6-28: Bottom View - Lamp PCB Cover Screws

4. Remove cover.

Note: Heat sink will be removed as a result of removing the two side screws.

Ground Screw

Two Screws Backside (Not Shown)


6-16 100-2414D 09/05

5. Remove the two remaining screws securing the PCB (Figure 6-29).

Figure 6-29: Lamp Control PCB

6. Remove three cables to lamp control PCB and lift out.

7. Install new lamp control PCB by securing with two mounting screws and attaching the three cables.

8. Install the cover and heat sink. For ease of assembly, position the heat sink in place prior to placing over lamp control PCB (Figure 6-30).

Figure 6-30: Position Heat Sink

9. Install the two screws heat sink side first, then the two remaining screws securing the cover.

10. Reassemble in reverse order of the above steps.


100-2414D 09/05 6-17

6.3.12 Power Supply, 12 Volt (9310178)

1. Open top cover.


3. Remove four screws securing lamp housing and remove (Figure 6-31).

Figure 6-31: Lamp Housing Mounting

4. Remove all cables to 12 volt power supply.

5. Remove four mounting screws and remove power supply (Figure 6-32).

Figure 6-32: 12 Volt Power Supply Mounting

6. Install new 12 volt power supply in reverse order of the above steps.


6-18 100-2414D 09/05

6.3.13 Power Supply, 24 Volt (9310179)

1. Open top cover.


3. Remove four screws securing lamp housing and remove (Figure 6-33).

4. Remove all cables to 24 volt power supply.

5. Remove four mounting screws and remove power supply (Figure 6-33).

Figure 6-33: 24 Volt Power Supply Mounting

6. Install new 24 volt power supply in reverse order of the above steps.


100-2414D 09/05 6-19

6.3.14 Photo Sensor - Plate Carrier/Analog Board (9310174)


2. Open the clip securing the optical fiber assembly. Remove the three screws securing the cover and remove (Figure 6-34).

Figure 6-34: Remove Clip and Mounting Screws

3. Remove the screw securing the photo sensor and remove (Figure 6-35).

Figure 6-35: Removing the Photo Sensor

4. Install new photo sensor in reverse order of the above steps.

5. Check plate positioning per Section 7.5.


6-20 100-2414D 09/05

6.3.15 Photo Sensor - Filter Wheel (9310174)


2. Remove cable from photo sensor. Remove screw securing photo sensor and remove (Figure 6-36).

Figure 6-36: Photo Sensor Removal

3. Install new photo sensor in reverse order of the above steps.


100-2414D 09/05 6-21

6.3.16 Model 680 Light Balancing Filter (9310173)


2. Remove plastic cover and metal cover from atop lamp.

3. Remove two screws securing lamp housing (Figure 6-37).

Figure 6-37: Lamp Housing

4. Remove two screws securing the light balancing filter (Figure 6-38).

Figure 6-38: Light Balancing Filter

5. Install new light balancing filter in reverse order of the above steps.


6-22 100-2414D 09/05

6.3.17 Model 680 XR Light Balancing Filter (9310275)


2. Remove two screws (arrows) securing lamp housing assembly (Figure 6-39).

Figure 6-39: Lamp Housing

3. Remove two recessed screws securing the light balancing filter (Figure 6-40).

Figure 6-40: Light Balancing Filter

4. Install new light balancing filter in reverse order of the above steps.


100-2414D 09/05 6-23

6.3.18 Plate Carrier Motor (9310172)


2. Remove the three cover screws and lift away (Figure 6-41).

Figure 6-41: Remove Inner Cover

3. Remove the two screws from the rail cover and remove cover (Figure 6-42).

Figure 6-42: Cover Screws


6-24 100-2414D 09/05

4. Loosen the screw to the belt and remove belt Figure 6-43).

Figure 6-43: Loosen Belt Adjustment Screw

5. Remove two screws securing motor (Figure 6-44). Remove and unplug motor.

Figure 6-44: Motor Mounting Screws

6. Install new motor in reverse order of the above steps.


100-2414D 09/05 6-25

6.3.19 Optical Fiber Assembly (9310169)

1. Open top cover.


3. Open the clip securing the optical fiber assembly. Remove the three screws securing the cover and remove (Figure 6-45).

Figure 6-45: Removing Optical Fiber Assembly

4. Remove two screws securing the cover over the analog board

5. Carefully remove fiber from mounting clips (Figure 6-46).

Figure 6-46: Remove Cover Screws

6. Install new optical fiber assembly in reverse order of the above steps.


6-26 100-2414D 09/05

7. When re-installing fiber into filter wheel housing, ensure that end is protruding 2 mm from plate (Fig-ure 6-47).

Figure 6-47: Fiber Installation


100-2414D 09/05 6-27

6.3.20 Filter Wheel (9310170) & Filter Wheel Motor (9310171)

1. Open top cover.


3. Cut cable tie and remove two screws securing the filter wheel assembly (Figure 6-48).

Figure 6-48: Filter Wheel Assembly Mounting

4. Unplug cable from motor to analog board.

5. Remove two screws securing filter wheel to motor (Figure 6-49).

Figure 6-49: Filter Wheel Mounting

6. Remove two screws securing motor to mounting bracket.

7. Install new filter wheel or motor in reverse order of the above steps.


6-28 100-2414D 09/05

6.3.21 Fan (9310168)

1. Open top cover.


3. Remove 4 screws and move lamp assembly in order to facilitate unpluging of fan cable (Figure 6-50).

Figure 6-50: Lamp Assembly Mounting

4. Remove four mounting screws and remove fan (Figure 6-51).

Figure 6-51: Fan Mounting

5. Install new fan in reverse order of the above steps.


100-2414D 09/05 6-29

6.3.22 Lithium Battery (9006020)

1. Open top cover.


3. Replace the lithium battery located on the CPU board (Figure 6-52).

Figure 6-52: Lithium Battery

4. Install new lithium battery in reverse order of the above steps.

Adjustments, Calibration and Checks

100-2414E 02/06 7-1

Section 7Adjustments, Calibration and Checks

7.1 Introduction

This section provides the adjustments, calibration and checks applicable to the Model 680 and Model 680 XRMicroplate Readers.


Standard Hand Tools

Voltmeter

9310183 Incubator Adjustment Tool

9310184 RS-232C Cable

1002446 Firmware CD

7.3 Electronics Adjustment

7.3.1 Setting of Jumper on the CPU Board (#281)

1. Open the top cover and remove plate carrier assembly to access CPU Board (#281).

2. Locate JP2 (Figure 7-1).

Figure 7-1: Jumper JP2

3. Set the jumper on the CPU Board (#281) per the following table:

Table 7-1: JP2 Jumper Settings for CPU Board

Instrument Configuration

Jumper Jumper Placement

Without Incubator JP2 Jump

With Incubator JP2 No Jumper (Open)


7-2 100-2414E 02/06

7.3.2 Setting of the Jumpers on the Printer Control Board (#284)

1. Open the top cover to access Printer Control Board (#284).

2. Check printer head type on flexible cable (Figure 7-2).

Figure 7-2: Location of Head Type

3. Configure the jumpers to JP4 in accordance with the Figure 7-3.

Figure 7-3: JP4 Jumper Settings


100-2414E 02/06 7-3

7.3.3 Incubator Electronics Adjustment (Board #284)

Note: This procedure is used to set the proper temperature set points for the incubator.

1. Open the top cover and remove plate carrier assembly to access Incubator board (#284).

2. Disconnect cable from plate carrier to CN52.

3. Connect cable into Incubation Adjustment Tool, 9310183, 107.79 ohm connector on tool.

4. Turn on instrument.

5. Measure voltage between TP5 and TP0 (Figure 7-4).

Figure 7-4: Incubator Board Adjustments


7. Connect cable into Incubation Adjustment Tool, 116.47 ohm connector on tool.

8. Measure voltage between TP5 and TP0.


10. If voltage is out of range, repeat steps 3 through 9.


7-4 100-2414E 02/06

7.4 Adjusting Plate Carrier Belt Tension

1. This procedure is used to adjust the belt tension of the plate carrier.

2. Open the top cover.

3. Remove fiber optics cover (Figure 7-5)

Figure 7-5: Remove Fiber Optics Cover

4. Remove two screws securing the belt cover as shown in Figure 7-6.

Figure 7-6: Remove Belt Cover


100-2414E 02/06 7-5

5. Loosen the screw and adjust the belt tension. Tension should be firm but not overly tight.

Figure 7-7: Belt Tension Adjustment


7-6 100-2414E 02/06

7.5 Alignment of Plate Carrier

7.5.1 Checking Plate Alignment

1. This procedure is used to check positioning and alignment of the plate carrier.


3. Install Checkmark Plate into reader. Make sure plate is properly seated.

4. Apply clear tape over small holes in columns 1 and 12 (Figure 7-8).

Figure 7-8: Placement of Tape

5. Use QC software to move plate beneath optics path per Section 7.7.3.

6. Check that the light is centered about the holes in the Checkmark Plate in columns 1 and 12 (Figure 7-9).

Figure 7-9: Checking Plate Position at Columns 1 & 12


100-2414E 02/06 7-7

7.5.2 Adjusting Plate Alignment - Y Direction

1. This procedure is used to adjust the Y direction (top/bottom) position of the plate carrier by reposi-tioning of the rails.


3. Loosen the 12 screws shown in Figure 7-10 and adjust the rails as required.

Figure 7-10: Y Direction Adjustment - Reposition Rails

7.5.3 Adjusting Plate Alignment - X Direction

1. This procedure is used to adjust the X direction (left/right) position of the plate carrier by repositioning the photo sensor.


3. Loosen the screws shown in Figure 7-11 and reposition the photo sensor as required.

Figure 7-11: X Direction Adjustment - Reposition Sensor


7-8 100-2414E 02/06

7.6 Firmware

7.6.1 General

1. Downloading firmware is achieved through a PC (Windows 95, NT, 2000, XP) and an RS-232 cable.

2. The communication requirements are as follows:

RS232CPort: COM1Baud Rate: 9600, 57600Data: 8 bit 1 stop

3. The downloading program and additional files are required for downloading:

m680_up.exe mscomctl.ocxcomdlg32.ocx msvbvm60.dllmscomm32.ocx asycfilt.dllcomcat.dll mci32.ocxoleaut32.dll olepro32.dllvb6stkit.dll

4. The current firmware files are:

M680_V171OL.txt for Original Lens models (SN 10001 through 10735)M680_V171NL.txt for New LCD models (SN 10736 through 13850)M680_V171.txt for Backlit LCD models (SN 13851 and higher) M680_V171.txt for Chinese version instrumentsM680XR_V163-XR.txt (for Model 680XR readers)

5. When updating firmware versions 1.60 and earlier with m680_up.exe, plate reading results, security password, Checkmark reference values, Checkmark reading results and laboratory name will be lost. Protocol settings (except for #5-8) and filter wavelengths will be retrieved and restored in the update process. Date and time will need to be reset.

6. When updating firmware versions 1.61 and later with m680_up.exe, all data and settings except for security password, Checkmark reading results, Protocol settings (except for #5-8) and Plate Reading results (#5-8) be retrieved and restored in the update process. Date and time will need to be reset.


100-2414E 02/06 7-9

7.6.2 Firmware HistoryTable 7-2: Firmware History

Instrument Style

Firmware Version

History

Compatibility

Original LCD

New LCD

Backlit LCD

Original LCDSerial Numbers 10001 - 10735

1.59 Initial release (compatible with serial num-bers 10001 through 10735)

Yes No No

1.60 Minor corrections (compatible with serial numbers 10001 through 10735)

Yes No No

1.62 Added TSE protocols (compatible with serial numbers 10001 through 10735)

Yes No No

1.63 Changed TSE validation criteria (compatible with serial numbers 10001 through 10735)

Yes No No

1.71OL Added functionality for ESC/P printer. Added protocols for TSE Sheep/Goat and Rabies (compatible with serial numbers 10001 through 10735)

Yes No No

New LCDSerial Numbers 10736 - 13850

1.60NL Released for new LCD display (SN 10736 through 13850). Minor corrections.

No Yes No

1.60cNL Released for Chinese version readers (SN 11201 through 13850).

No Yes No

1.62NL Added TSE protocols (compatible with serial numbers 10736 through 13850)

No Yes No

1.63NL Changed TSE validation criteria (compatible with serial numbers 10736 through 13850)

No Yes No

1.71NL Added functionality for ESC/P printer. Added protocols for TSE Sheep/Goat and Rabies (compatible with serial numbers 10736 through 13850)

No Yes No

Model 680XR 1.63XR For Model XR functionality only No Yes No

Backlit LCDSerial Numbers

13851 and higher

1.70 Released for backlit LCD and integration of English and Chinese version firmware. Added functionality for ESC/P printer (effec-tivity serial number 13851 and higher).

No No Yes

1.71 Added protocols for TSE Sheep/Goat and Rabies (effectivity serial number 13851 and higher).

No No Yes


7-10 100-2414E 02/06

7.6.3 Integration of English and Chinese Firmware Versions

Note: With firmware version 1.70, the English and Chinese versions have been integrated. A language choice has been added with this version.

1. To enter the Select Language screen, press the 0 key and 4 key and turn ON instrument power. Hold for approximately 5 seconds and the Select Language screen will appear.

2. Use Up and Down arrow keys to select language choice. Press Enter.

3. Reader resets automatically and restarts with the selected language.

Note: If the language selected is different, the reader will clear all memory and restore default values.


100-2414E 02/06 7-11

7.6.4 Downloading Firmware

1. Load the m680_up.exe program. The screen shown in Figure 7-12 will appear.

Figure 7-12: Microplate Reader Model 680 Update Screen

2. Click on Model 680 Update and the following screen will appear:

Figure 7-13: Click Yes after Log-in

3. Connect a RS-232C cable between Model 680 and PC COM1 port.

4. Turn ON Model 680 reader and input password for log in (default 00000). Press enter.

5. When complete, press Yes button on screen.


7-12 100-2414E 02/06

6. The following screen will appear:

Figure 7-14: Confirmation Screen

7. Press Yes to update to new version. If the instrument is at the latest version, the screen will indicate no update is required.

8. The screen shown in Figure 7-15 will indicate the status as existing data and settings are retrieved from the Model 680 reader.

Figure 7-15: Retrieving Data Screen


100-2414E 02/06 7-13

9. When data retrieving is complete, the following screen will appear:

Figure 7-16: Firmware Update Screen

10. The following steps are also indicated on the screen shown in Figure 7-16

11. Turn OFF the Model 680 Reader.

12. While pushing both Main and 0 key on the keypad, turn on reader power switch. After about 10 seconds, the following screen on LCD appears:

Firmware updateAre you sure?Yes: Start KeyNo: 7 Key

13. Push Start/Stop key switch on the Model 680 reader to begin reader firmware erasing.

14. After the erasing finishes, the LCD screen RS Receive will appear.

15. Click the Start button on PC screen. The firmware will begin uploading and bar graph at the bottom of the screen will display status. This will take approximately 7 minutes.

16. When the firmware update operation is complete, the LCD on the reader will displayed Finished and the restore previous data screen will appear (Figure 7-17).


7-14 100-2414E 02/06

Figure 7-17: Restore Previous Data Screen

17. Turn OFF reader power switch then turn ON. A Battery Low error message will occur. This is nor-mal; press Enter.

18. At the Calendar settings screen, enter Date and Time and press Enter.

19. Enter password and press Enter.

20. After completion of log-in press the Yes button on the PC screen.

21. The restoring data screen will appear (Figure 7-18).

Figure 7-18: Storing Data Screen


100-2414E 02/06 7-15

22. When the update is complete, the firmware complete message will appear (Figure 7-19).

Figure 7-19: Firmware Update Complete

23. Click OK to continue.

24. A password change reminder screen will appear (Figure 7-20).

Figure 7-20: Change Password Reminder Screen

25. Follow on screen instructions to change password or select Exit to quit.


7-16 100-2414E 02/06

7.7 QC Software

Note: There are 4 modes in this program; Measure Mode, Monitor Mode, Extension Mode and Incubator. The instructions in this section apply to the Monitor Mode which allows step-by-step positioning of the plate carrier.

7.7.1 Entering QC Software

1. While pressing the 0 and Upper key buttons, turn ON power switch. Continue to hold down but-tons until QC Software screen appears.

2. Select YES by pressing the 1 key.

3. Input the password 00000 and press Enter.

4. Press the Dot key . to enter the QC Mode menu (Figure 7-13).

Figure 7-21: QC Mode

7.7.2 Monitor Mode

1. Select Monitor Mode from the menu by pressing 2 (Figure 7-14).

Figure 7-22: Monitor Mode

Definitions:MON = AD Value (voltage conversion)DEC = AD Value (decimal)CH = Row PositionPLT = Plate Column PositionFL = Filter NumberGain = Gain A, B

Commands:Main = Back to QC Mode Screen

Change of Gain A

Change of Gain B

Change of Plate Position

Change of Filter Position

. (Dot) = Change of Channel

QC 1: Measure Mode2: Monitor Mode3: Extension Mode4: Incubator

MON: VDEC:CH: A PLT: 12FL: 1 Gain=1.1.1.0


100-2414E 02/06 7-17

7.7.3 Plate Positioning

1. To use the QC software to check for plate positioning, enter the QC Mode and press 2 for Monitor Mode.

2. The reader will initialize the plate carrier and place column 12 in the optics path.

3. Press the down arrow key to change filter position for desired filter position.

4. Press the up arrow key to step the plate through column positions 11 to 1.

7.8 External Printer Setup

Note: The Model 680 Reader with firmware version 1.70 added support for ESC/P external printer mode. Previous versions of firmware support only ASCII print mode.

1. To change the external printer print mode, perform the following steps:

a. Select Edit from the top screen menub. Select Printerc. Select desired print mode: ASCII or ESC/Pd. Press Enter

2. Connect printer and check print application by performing any run.

Troubleshooting

100-2414E 02/06 8-1

Section 8Troubleshooting

8.1 About this Section

This section of the manual provides information on troubleshooting the Model 680 and 680 XR MicroplateReader. The information in this section will help diagnose the common operating difficulties and componentfailures. If additional assistance in resolving a problem is required, please contact the Technical ServiceDepartment at 1-800-424-6723.

8.2 Troubleshooting

The following troubleshooting tables are intended as a guide to assist in diagnosing some common problems.

Table 8-1: General Problems

Problem Possible Cause Solution

Will not communicate with com-puter

Improper COM Port selected Check that COM1 port is used.

Improper serial cable Check for proper serial cable .

CPU board problem Replace CPU board.

Blank LCD upon Power Up Fuse Blown Check Fuse. If bad, replace

Cable connection Check display cable and reseat.

Bad LCD Replace LCD.

Memory Error Bad CPU Board Replace CPU Board.

Calendar Error Data error Reset time and date. Reboot.

Bad CPU Board Replace CPU Board.

Printer Error Printer malfunction Replace printer.

Will not print to external printer Check printer setup Section 7.8 (ESC/P requires firmware ver-sion 1.70 or later)

Battery Error Battery dead Replace battery.

Old version firmware Download new firmware.

LCD Hardware Error LCD malfunction Replace LCD.

A/D Conversion Error Loose cable connection Check cables to CPU board and Analog Board

Bad A/D convertor Replace Analog Board.

Grinding Sound or Plate Error Carrier binding Check for source of binding. Clean and lubricate plate carrier rods as necessary.

Troubleshooting

8-2 100-2414E 02/06

Table 8-2: Error Codes


Code 8071- Invalid Command Received data error Resend command

Code 8072 - Parameter Out of range

Received command parameter is out of range

Resend command and parame-ter

Code 8073 - Not in Remote Mode

Instrument is not in Remote Mode

Send the AQ command to acquire Remote Control Mode

Code 8074 - Device Busy Instrument is busy Wait until ready

Code 8075 - Filter Disk Jammed CPU board bad Replace CPU board

Motor bad Replace motor

Photo interrupter for Filter disk damaged

Replace photo interrupter

Filter disk damage Replace the filter disk

Code 8076 - Carrier Jammed After Reading

Plate is not properly seated Reseat plate

Interference or blockage Check for Interference or block-age

Code 8077 - Light Bulb Error Lamp defective Replace lamp

Lamp out of alignment Check that lamp is properly seated

Optical fiber damaged Replace optical fiber

Bad analog board Replace Analog Board

Code 8082 - Filter Wheel Filter wheel jammed Check filter wheel

Code 8083 - Incubator Error Thermo sensor damaged Replace plate carrier

Incubation control board bad Replace Incubation control board

Code 8084 - Carrier Home Sen-sor Closed Status Error

Photo interrupter for plate carrier damaged


CPU board bad Replace CPU board

Code 8085 - Carrier Home Sen-sor Open Status Error


Interference or blockage Check for Interference or block-age

Photo interrupter for plate carrier damaged




Troubleshooting

100-2414E 02/06 8-3

Code 8086 - Carrier Jammed Before Reading




Code 8087 - Plate Carrier Posi-tion

Plate carrier jammed after read-ing

Check plate carrier for obstruc-tion.

Code 8088 - Light Level Error Between Channel

Dirty Lenses Clean lenses

Optical fiber damaged Replace optical fiber

Bad analog board Replace Analog Board

Code 8089 - Filter Missing Filter missing from filter wheel allowing light to exceed accept-able range

Check filter wheel and install filter

Code 8090 - Security Code in Error

Security code did not match Remove security lock

Code 8091 - RS-232C Error Data communication problem Check RS-232 cable

Code 8092 - A/D Conversion Error

Bad A/D convertor Replace Analog Board

Code 8093 - Stop Key Pressed Stop key pressed during mea-surement

User error. Reset command

Table 8-2: Error Codes


Troubleshooting

8-4 100-2414E 02/06

Table 8-3: Firmware Downloading


Checksum Error Corrupted Firmware File Repeat using uncorrupted file

Communication Time Out RS-232 not connected Ensure RS-232 is properly con-nected.

Wrong button clicked Repeat download procedure

RS-232C Error Parity, Overrun or Framing errors occurred

Ensure RS-232 is properly con-nected. Repeat download proce-dure

Verify Error Communicated data and written data is different

Repeat download procedure (make sure instrument power is cycled)

Flash Write Error Flash memory could not be writ-ten

Repeat download procedure (make sure instrument power is cycled)

Flash Erase Error Flash memory could not be erased

Repeat download procedure (cycle power; first screen to appear is downloading screen)

Erase Check Error No erasing memory exists Repeat download procedure (make sure instrument power is cycled)

Battery Error Occurs after firmware download Normal. Press Enter. Reset date, time and filter data.

Troubleshooting

100-2414E 02/06 8-5

Table 8-4: Checkmark Plate Failures


Fails Accuracy, Linearity or Precision

Erratic or non-uniform well-to-well OD values

Check for cleanliness of optics and lenses. Clean as required.

Check lamp. Reseat or replace as necessary.

Check filter. Reseat or replace as necessary.

Replace fiber optics assembly

Replace analog board

Replace CPU board

Fails Plate Positioning Plate binding Check for proper movement of plate.

Belt tension too tight or too loose Check belt tension and tighten/loosen as necessary per Section 7.4

Plate carrier out of alignment Check plate alignment and cor-rect as necessary per Section 7.5

Failed Cross Talk Wells A2, C2, E2, G2, B3, D3, F3, and H3 are exceeding 0.003 OD due to some light path blockage.

Check for cleanliness of optics and lenses. Clean as required.

Check lamp. Reseat or replace as necessary.

Check filter. Reseat or replace as necessary.

Check for proper movement of plate.

Check belt tension and tighten/loosen if necessary per Section 7.4

Check plate alignment and cor-rect as necessary per Section 7.5

Replace fiber optic assembly

Fails Filter Check Filter deterioration Replace filter

Fails 0 OD/Overflow Not detecting infinite absorbance Check plate alignment and cor-rect as necessary per Section 7.5

Replace analog board

Fails IR Blocking Detected IR light passing through plate; not blocked by filters

Check filter for damage or deteri-ation. Replace as necessary.

Parts Information

100-2414E 02/06 9-1

Section 9Parts Information

9.1 About this Section

This section of the manual provides information on the field replaceable parts and service tools for the Model680 and 680 XR Microplate Readers. Information on parts and tools ordering is also included in this section.

9.2 Parts Orders

9.2.1 US Domestic Operations

Parts may be ordered through our Service Parts department. You can reach the Service Parts department during Pacific time regular office hours by calling 1-510-741-5038 or 5058. You can help to make processing your order more efficient by having the following information before you call:

The part number(s) or catalog number(s) of the parts you want to order

Shipment information

Your account or inventory location number

9.2.2 International Subsidiaries

The procedures for ordering parts vary from region to region outside the United States. Please contact your Bio-Rad Export Sales Representative through your regular sales and service channels.

9.3 Returning Defective Parts

9.3.1 US Domestic Operations

Defective Assemblies returned for evaluation and repair are an important part of the logistics and inventory control process. Returned parts and assemblies are refurbished when possible, tested and returned to ser-vice stock. Maintaining the effectiveness and efficiency of this process enables us to deliver the high standard of service that our customers expect, at a reasonable cost that allows us to remain competitive.

You are an integral part of our inventory process. When you return parts or assemblies to Bio-Rad to be eval-uated and repaired, please attach a tag to each assembly. The tag should contain the following information:

Model and serial number of the unit which the part was removed

The part number or catalog number of the part being returned

The date of removal

A description of the problem or symptoms exhibited

Your name and other identification information

This information will help our service centers to make repairs efficiently. When you receive replacement parts,sa

Фотометр для микропланшет модель 680 & 680 xr (bio-rad) СИ.pdf

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