8 genetic notes 1/22 9 lab activity: predicting genetic ratios 1/22
TRANSCRIPT
![Page 1: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/1.jpg)
TOC
8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios
1/22
![Page 2: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/2.jpg)
GENETICS
the branch of biology that studies the transmission of hereditary information from parents to offspring
![Page 3: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/3.jpg)
GREGOR MENDEL AND HIS PEA PLANTS EXPERIMENTS (1857-1865)
![Page 4: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/4.jpg)
WHY MENDEL? WHY PEAS?
Mendel was a monk who was educated--he knew MATH! He also had a lot of free time.As for the peas? Well, they were:* easy to grow* inexpensive* easy to pollinate (either self or cross)* easy to study their TRAITS (characteristics) such as: height, seed pod shape, seed color, seed pod color, seed texture, flower position, and seed shape
![Page 5: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/5.jpg)
Mendel kept very accurate records for hundreds of individual plants.
He used statistical analysis (MATH) to study the traits of different generations.
Through his findings, he proposed that the traits were passed on by some kind of hereditary factors (now know to us as DNA).
![Page 6: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/6.jpg)
HERE'S BASICALLY WHAT HE DID One trait he studied was plant height. Mendel pollinated all tall plants for many
generations to get a pure population of tall plants. He also pollinated all short plants to get a pure population of small plants. See picture below...
X means to cross(as in cross-pollinate)
tall plants X tall plants as well as short plants X short plants
He then cross-pollinated a member of the tall pure population with a member of the short pure population...
P1 (pure parent generation)P1 TALL PLANT X P1 SHORT PLANT
ALL TALL PLANTS
F1 (first filial [family] generation)Where did the short trait go? Mendel then crossed two tall members of the F1 generation.
F1 TALL PLANT X F1 TALL PLANTTHIS IS CALLED A MONOHYBRID CROSS (hybrids known for one trait)
F2 (second filial generation)For every 3 TALL PLANTS there was 1 SHORT PLANT
![Page 7: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/7.jpg)
So, the short trait did not disappear. It was present in the F1 generation, but IT WAS NOT EXPRESSED (this means it did not show up!) Somehow, it was expressed (visible) in the F2 generation.
![Page 8: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/8.jpg)
MENDEL'S PRINCIPAL OF DOMINANCE
because the tall trait showed up more than short, Mendel call this trait DOMINANT
the short trait, because it seemed 'weaker' than the tall trait, was called RECESSIVEAfter this discovery...well, um...NOBODY CARED! Later on, when scientists studies
meiosis in Drosophila (genus name for fruit flies), they linked together Mendel's factors
with the chromosomes in gametes
![Page 9: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/9.jpg)
Mendel's factors are now called GENES genes are segments of chromosomes
(DNA) that code for a characteristic these characteristics can be:
a) physical traits (can be seen like eye color, hair color, height, etc.)
b) chemicals produced in the body (like for hormones or enzymes)
![Page 10: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/10.jpg)
genes are lined up on chromosomes in a certain order, like beads on a string
homologous (similar) chromosomes have the same order of genes...HOWEVER..
these chromosomes might have the different forms of a gene ex. trait=eye color can be blue, black, brown, hazel, green, etc.
these different forms of a gene are called alleles in simple patterns of inheritance, there are 2 different
forms of a gene (alleles), where one is dominant and one is recessive
DOMINANT IS SHOWN BY CAPITAL LETTERS recessive is shown by lowercase letters
ex. in Mendel's peas T = tall t = short
![Page 11: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/11.jpg)
diploid (having 2 sets of chromosomes) organisms have 2 copies of genes, one on each chromosome they have the 2 copies can be the same--
HOMOZYGOUS, like TT (homozygous dominant) or tt (homozygous recessive)
the 2 copies can be different--HETEROZYGOUS Tt (heterozygous)
![Page 12: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/12.jpg)
NOTICE THAT WHAT THE ORGANISM LOOKS LIKE AND THE KIND OF GENES IT HAS ARE TWO SEPARATE WAYS TO CLASSIFY AN ORGANISM
phenotype: what the appearance of an organism is (how it looks) ph = physical
genotype: what the genetic makeup of an organism is (what genes it has) gen = genes
genotype can be: homozygous dominant----------> TT
heterozygous (a hybrid--a mix)---> Tthomozygous recessive----------> tt
recessive phenotypes can only be expressed (show up) when there are 2 copies of a recessive gene present at the same time...if a dominant gene was there, then that would be expressed, hiding the recessive gene!
![Page 13: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/13.jpg)
MENDEL'S PRINCIPLE OF SEGREGATION AND RECOMBINATION
when gametes are formed during meiosis (DIPLOID to HAPLOID) the homologous chromosomes (which contain the many different genes) separate randomly this is called SEGREGATION
![Page 14: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/14.jpg)
if the organism has a homozygous (the same) genotype for a given trait (TT or tt), then all gametes will also have that trait
TT <----diploid----> ttmeiosis
T or T <----haploid----> t or t if the organism has a heterozygous (not the same)
genotype for a given trait (Tt), then half of the gametes will have one trait, and the other half will have the other trait
Tt <----diploidmeiosis
T or t <----haploid when the haploid gametes fuse during fertilization, the
diploid number of chromosomes is restored this can result in a new combination of genes this is called RECOMBINATION
![Page 15: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/15.jpg)
LET'S LOOK BACK AT MENDEL'S FIRST FEW PEA PLANT CROSSES
P1 (pure parent generation) cross was: TT X tt
F1 (first filial generation) cross was Tt X Tt
F2 (second filial generation) offspring had genotypes of TT, Tt, tT, and tt
![Page 16: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/16.jpg)
NOW, COMPARE THE DIFFERENT PHENOTYPES (PHYSICAL APPEARANCE) AND GENOTYPES (GENETIC MAKEUP).
For the F2 generation what is the PHENOTYPIC RATIO?
3 tall : 1 short
For the F2 generation, what is the GENOTYPIC RATIO? 1 homozygous dominant : 2 heterozygous : 1 homozygous
recessive
PHENOTYPE DOES NOT ALWAYS EQUAL GENOTYPE!
![Page 17: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/17.jpg)
TO SHOW SEGREGATION (SEPARATION OF GENES/CHROMOSOMES) AND RECOMBINATION (DURING
FERTILIZATION), WE USE PUNNETT SQUARES
![Page 18: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/18.jpg)
PUNNETT SQUARE METHOD
Problem: A homozygous tall pea plant is crossed with a short pea plant. (Remember, tall is dominant over short for pea plants!) What would the expected genotypes (the genotypic ratio) and phenotypes (the phenotypic ratio) of their offspring?
![Page 19: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/19.jpg)
STEP 1: SET UP THE KEY THAT WILL SHOW HOW EACH ALLELE (FORM OF A GENE) WILL BE SHOWN
T = tallt = short
![Page 20: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/20.jpg)
STEP 2: SET UP THE PARENTS THAT WILL BE CROSSED; USE THE INFORMATION FROM THE WORD PROBLEM ITSELF
TT x tttall homozygous parent & short parent
* remember, to be short, it has to have both short alleles!
![Page 21: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/21.jpg)
STEP 3: do the Punnett square; that is, put one parent on the top and the other parent on the left of the square...then from a blank box, take the allele on the top and the allele from the left and fill them in
![Page 22: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/22.jpg)
STEP 4: SHOW THE RESULTS (RATIOS) OF THE CROSS
GENOTYPIC RATIO: Tt, Tt, Tt, Tt
PHENOTYPIC RATIO: 4 Tall
Homework: Complete handout on Monohybrid crosses and online questions. Review notes and be prepared for quiz.
![Page 23: 8 Genetic Notes 1/22 9 Lab Activity: Predicting Genetic Ratios 1/22](https://reader036.vdocuments.net/reader036/viewer/2022062423/56649cf65503460f949c5818/html5/thumbnails/23.jpg)
TRY THESE PUNNETT SQUARE PROBLEMS
1) Use a Punnett square to show the offspring of a cross between two pea plants that are heterozygous for height (Tt). Give the phenotype and genotypes of the offspring. (That is, what is the phenotypic and genotypic ratios!)
2) Use a Punnett square to show the offspring of a cross between a pea plant that is homozygous tall and one that is heterozygous. Give the phenotypes and genotypes of the offspring.
3) Use a Punnett square to show the offspring of a cross between a pea plant that is heterozygous tall and one that is homozygous recessive for height. Give the phenotypes and genotypes of the offspring.