免疫学信息网 . rf-c is a five-subunit complex all subunits are related in sequence and have...

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Page 1: 免疫学信息网 . RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis

免疫学信息网 http://www.immuneweb.com

Page 2: 免疫学信息网 . RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis

免疫学信息网 http://www.immuneweb.com

Page 3: 免疫学信息网 . RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis

免疫学信息网 http://www.immuneweb.com

Page 4: 免疫学信息网 . RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis

免疫学信息网 http://www.immuneweb.com

Page 5: 免疫学信息网 . RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis

免疫学信息网 http://www.immuneweb.com

RF-C is a five-subunit complexAll subunits are related in sequence and have ATP binding motifsATP hydrolysis by RF-C is associated with the loading of PCNARF-C is the functional homolog of the clamp-loader complex

Page 6: 免疫学信息网 . RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis

免疫学信息网 http://www.immuneweb.com

Page 7: 免疫学信息网 . RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis

免疫学信息网 http://www.immuneweb.com

Polymerase switching occurs even on lagging strands; pol does most of DNA synthesis

Page 8: 免疫学信息网 . RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis

免疫学信息网 http://www.immuneweb.com

Page 9: 免疫学信息网 . RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis

免疫学信息网 http://www.immuneweb.com

How do you think the PCNA is removed after ligation?

Page 10: 免疫学信息网 . RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis

免疫学信息网 http://www.immuneweb.com

Page 11: 免疫学信息网 . RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis

免疫学信息网 http://www.immuneweb.com

PCNA interacts with RF-C, pol , Fen1, DNA ligase,CAF1 and MCMT

Several of these have a common motifused in the interaction: Q-X-X-L/I/M-X-X-F-F/Y

p21/CIP1/WAF1, a protein induced by the tumor suppressor p53 uses the same motifto interact with PCNA

What effect is p21 expected to have on DNA replication/repair?

Page 12: 免疫学信息网 . RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis

免疫学信息网 http://www.immuneweb.com

FEN1 interacts with PCNA

Page 13: 免疫学信息网 . RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis

免疫学信息网 http://www.immuneweb.com

DNA Helicase

* Helicase binds ss DNA

* Hydrolyzes ATP to move alongssDNA and peel of substrate DNA

* Can move 5‘ to 3’ or 3‘to 5’ or in both directions on ssDNAand is classified as such

* Dimers or hexamers

P P

5‘ 3‘

3‘ 5‘ 5‘3‘

Substra

Boiled su

bstra

+he

licas

+helic

ase+

ATP

15mer 30mer

15mer30mer

Page 14: 免疫学信息网 . RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis

免疫学信息网 http://www.immuneweb.com

Conserved helicase motifs:

Includes the following for ATPbinding and hydrolysisI = Walker A motif GxGxGKTII = Walker B motif DEad

Binds ATP

Tight conformation

Hydrolyzes ATP

Relaxed conformation

Helicases are ATP driven molecular motors

Fast ATP-dependent Subunit Rotation in Reconstituted FoF1

HETEROTRIMERIC G-PROTEIN ALPHA (α) SUBUNIT FROM A ...research.sabanciuniv.edu/34010/1/ErsoyColak_10111818.pdfcomplex is comprised of alpha (Gα), beta (Gβ) and gamma (Gγ) subunits;

c ring of A1AO ATP synthases A c subunit with four transmembrane

RESEARCH Open Access Evidence of inhibin/activin subunit ......This amino acid sequence is unique to the inhibinbE subunit and is absent from other inhibin subunits. This, as well

Regulation of Intracellular Cholesterol Distribution by ... · (1,2). The functional sodium pump is mainly comprised of α and β subunits. The α subunit is the catalytic component

Binding ADP + P i Synthesizing ATP Releasing ATP The binding-change mechanism or rotational catalysis (Paul Boyer, 1980s) Each subunit will take

hCG+ß-IRMA - diasource-diagnostics.com · hCG-molecular weight 37.9 kDa - comprises two subunits. The hCG α subunit -molecular weight 14.9 Kda - is chemically similar to the α

Potassium Channel Structure The subunit is formed from 6 transmembrane segments and is associated with a regulatory subunit. Four subunits

Metabolic Clearance Secretion Rates of Subunits · units had properties indistinguishable from those pre-viously prepared by our laboratory (1). Each subunit was labeled with either

Cyclic AMP-Dependent Protein Kinase Catalytic Subunits ...The divergent Pka2 catalytic subunit was found to regulate mating, haploid fruiting, and virulence factor production in serotype

Subunit structure of the vicilin-like globular storage ... all these subunits resist N-terminal sequencing by Edman degradation, we have ana- lysed the localisation of these vicilin

Limited proteolysis of E. coli ATP-dependent protease Lon – … · Limited proteolysis of E. coli ATP-dependent protease Lon – a unified view of the subunit architecture and characterization

O-linked N-acetylglucosamine transferase (OGT) interacts ... · The HIRA complex consists of three subunits, HIRA, UBN1, and CABIN1. To determine which subunit interacted with OGT,

LECTURE 10 NOTES ENZYMES - StudentVIP · Allosteric enzymes are made up of two subunits, the catalytic subunit (C) and the regulatory subunit (R). The modulator binds to the regulatory

Mediator subunit MED31 is required for radial patterning ...until 2007 (38). Despite the identification of a number of plant Mediator subunits that are implicated in the regulation

Chapter 2 - Particlesscitechconnect.elsevier.com/wp-content/uploads/2014/04/... · 2015. 3. 19. · (subunits/helix turn) Pitch of helix P = 2.28 nm p = 0.14 nm (axial rise/subunit)

The BAF60 Subunit of the SWI/SNF Chromatin-Remodeling ... · 07/02/2014 · the above-mentioned enzymatic ATPase, (2) core subunits, and (3) accessory subunits. In mammals, the chromatin-remodeling

RNA polymerase III subunit architecture and …C37/53 subcomplex and several TFIIE-related winged helix (WH) domainsinsubunitsC82andC34(14–20).TFIIEiscomposedof two subunits, Tfa1

BIOC/DENT/PHCY 230 LECTURE 2. Lactate dehydrogenase pyruvate + NADHlactate + NAD + M and H subunits: 5 isozymes M subunit has a lower affinity for pyruvate

Gene Structure of Human Mitochondrial ATP Synthase ?-Subunit · The human gene structure for the p- subunit and tissue-specific differences in its levels of mRNA expression have been

CHARACTERIZATION OF RESVERATROL BINDING SITES IN ATP SYNTHASE THROUGH MOLECULAR ANALYSIS OF CANDIDATE SUBUNITS Lauren Ivey Laura McCoy

Activation-triggered subunit exchange between CaMKII ... · unactivated molecules, and John Lisman later suggested that CaMKII could fulfil this role by swapping subunits of holoenzymes

potassium subunit to superfamily by Drosophila Hyperkinetic · FIG. 2. Amino acid sequence alignment for Hk(cDNAHC206) andthe rat,3 subunits KP/31 andK,I32 (3). Aminoacid identities

3D-localization of the a-subunit of F F -ATP synthase by ......3D-localization of the a-subunit of F oF 1-ATP synthase by time resolved single-molecule FRET Monika G. Düser a, Nawid

Structural Dynamics of Ribosome Subunit Association ...Ribosome subunit purification. Ribosome subunits of the tight-coupled 70S ribosomes were purified using sucrose density gradient

Downregulation of the d-Subunit Reduces Mitochondrial ATP Synthase

Elasticity, friction, and pathway of γ-subunit rotation in FoF1-ATP

ATP Synthase Subunit a Supports Permeability Transition in ...Mitochondrial ATP synthase, an enzyme that provides cellular energy in the form of ATP, is composed of 17 subunits [1]

Male Sterility (WA-CMS) system of L. subsp. indica in mitochondrial energy production and often involved ATP synthase subunit genes. Therefore, impaired ATP synthase activity could

The paracaspase MALT1 cleaves the LUBAC subunit HOIL1 ... · the LUBAC subunits in HEK293T cells (Elton et al., 2015). Whereas HOIL1Nter promotes LUBAC-mediated NF-κB signaling,

The Clathrin Adaptor Complex AP-2 Mediates Endocytosis of ... · subunits (a2 and b2 or AP2A and AP2B), one medium subunit (µ2 or AP2M), and one small subunit (s2 or AP2S) (Collins

TorqueGenerationandUtilizationinMotorEnzymeF0F1-ATP Synthase · Background: ATP synthase (F 0 F 1) is a rotary motor enzyme. Results: F 1 with a short-sized helix-1 in subunit rotates

Primers - NGRL · Web viewThe T8993G/C mutation affects the ATP synthase 6 subunit resulting in reduced ATP synthesis and is invariably heteroplasmic. It was first reported in 1990

Apicoplast - University of Massachusetts AmherstToxoplasma Plasmodium Eimeria Gene synteny Similarity to chloroplast genes 2 large subunit rRNA 2 small subunit rRNA 3 subunits of RNA

ab109716 – ATP Synthase Microplate Assay Kit Specific Activity · The mammalian ATP synthase is composed of 17 subunits. Five of the subunits make up the easily detached F1 domain