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Electron microscopy of particles of rice tungro virus complex R. G. Milne and G. Boccardo, Istituto di Fitovirologia applicata del Consiglio Nazionale delle Ricerche, Turin, Italy; and K. C. Ling, International Rice Research Institute Rice plants infected with ragged stunt virus at IRRI were homogenized in Turin. Two types of particles, apparently those of rice tungro virus complex, were found by electron microscopy, in addition to particles of ragged stunt virus, indicating that the diseased plants were multiple infected (IRRN 3[2]:9, 1978). The viruses were purified from the sap of the infected plants using Freon, Nonidet P40, ultra-centrifugation through sucrose cushions, and density gradient centrifugation in cesium sulfate. After negative staining, the preparation was examined under an electron microscope whose magnification was calibrated with a diffraction grating replica. The presumed tungro complex particles were isometric and bacilliform (see figure). The isometric particles were 27- 28 nm in diameter when negatively stained in 2% uranyl acetate (UA) and 30 nm in diameter in 1% neutral sodium phosphotungstate (PTA). The bacilliform particles were 23 nm in diameter in UA and 31-33 nm in diameter in PTA. The length of the bacilliform particles was variable in both stains. Apparently undamaged particles with both ends rounded were 110-190 nm long in UA, although no particular modal length was detected. Hibino and associates reported in Phytopathology 68:1412, 1978, on the negatively stained appearance and dimensions of particles of the tungro complex. They used PTA only on crude preparations. Our results in PTA confirm their measurements of particles in the same stain. However, we note that the bacilliform particles particularly appeared damaged in our PTA preparations. We think that the substantially greater diameter compared to that in UA was due to artificial swelling or flattening.

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Page 1: jameslitsinger.files.wordpress.com  · Web view05/06/1981 · The bacilliform particles were 23 nm in diameter in UA and 31-33 nm in diameter in PTA. The length of the bacilliform

Electron microscopy of particles of rice tungro virus complexR. G. Milne and G. Boccardo, Istituto di Fitovirologia applicata del Consiglio Nazionale

delle Ricerche, Turin, Italy; andK. C. Ling, International Rice Research Institute

Rice plants infected with ragged stunt virus at IRRI were homogenized in Turin. Two types of particles, apparently those of rice tungro virus complex, were found by electron microscopy, in addition to particles of ragged stunt virus, indicating that the diseased plants were multiple infected (IRRN 3[2]:9, 1978).

The viruses were purified from the sap of the infected plants using Freon, Nonidet P40, ultra-centrifugation through sucrose cushions, and density gradient centrifugation in cesium sulfate. After negative staining, the preparation was examined under an electron microscope whose magnification was calibrated with a diffraction grating replica.

The presumed tungro complex particles were isometric and bacilliform (see figure). The isometric particles were 27- 28 nm in diameter when negatively stained in 2% uranyl acetate (UA) and 30 nm in diameter in 1% neutral sodium phosphotungstate (PTA). The bacilliform particles were 23 nm in diameter in UA and 31-33 nm in diameter in PTA.

The length of the bacilliform particles was variable in both stains. Apparently undamaged particles with both ends rounded were 110-190 nm long in UA, although no particular modal length was detected.

Hibino and associates reported in Phytopathology 68:1412, 1978, on the negatively stained appearance and dimensions of particles of the tungro complex. They used PTA only on crude preparations. Our results in PTA confirm their measurements of particles in the same stain. However, we note that the bacilliform particles particularly appeared damaged in our PTA preparations.

We think that the substantially greater diameter compared to that in UA was due to artificial swelling or flattening.

Page 2: jameslitsinger.files.wordpress.com  · Web view05/06/1981 · The bacilliform particles were 23 nm in diameter in UA and 31-33 nm in diameter in PTA. The length of the bacilliform

Isometric and bacilliform particles of rice tungro virus complex.

Milne RG, G Boccardo, KC Ling. 1981. Electron microscopy of particles of rice tungro virus complex. International Rice Research Newsletter 6 (5) 13-14.


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