03-p102 expression and regulation of the claudin family of tight junction proteins during...
TRANSCRIPT
of ltk and melanoblast or xanthoblast markers can be found in
the embryo, consistent with a shared origin of these cell-types.
We also present evidence that iridophore precursors are likely
to also have glial potential. Finally, we engineered constitutively
active form of ltk gene by analogy with oncogenic gene nucleo-
phosmin-anaplastic lymphoma kinase, and examine the results
from experiments asking what happens to NCCs with constitu-
tively active Ltk signaling.
doi:10.1016/j.mod.2009.06.154
03-P102
Expression and regulation of the claudin family of tight junction
proteins during gastrulation
Michelle Collins, Aimee Ryan
McGill University, Montreal, Quebec, Canada
Research Institute of the Montreal Children’s Hospital, Montreal, Quebec,
Canada
During gastrulation epiblast cells undergo epithelial to mesen-
chymal transition (EMT) as they delaminate and ingress through
the primitive streak to form the definitive endoderm and meso-
derm. The dissociation of junctional complexes is essential for
EMT to occur. We are examining the expression and regulation
of the claudin family of integral tight junction proteins during
chick gastrulation. Tight junctions are localized to the apical pole
of epithelial cells and the claudin component of the tight junction
is responsible for determining the ion and size selectivity of the
junction. We hypothesize that claudin expression must be
repressed in the epiblast cells that ingress through the primitive
streak. More than 20 members of the Claudin family have been
identified in vertebrates. We found that Claudins -1, -3, -10, -11,
-12, -22 and -24 showed distinct expression patterns during gas-
trulation and we are now determining how these expression pat-
terns are regulated. For example, Claudin-1 is highly expressed in
the epiblast layer reduced in the cells that delaminate and ingress
through the primitive streak and absent from the nascent meso-
derm. The Claudin-1 promoter contains an evolutionarily con-
served E-box regulatory element that is recognized by members
of the Snail family of zinc-finger transcriptional repressors that
play a key role in EMT. We showed that the chick Claudin-1 pro-
moter is repressed by Snail2 in vitro, and that this interaction
occurs through binding to the E-box element. We are currently
investigating the role of Snail-2 in regulating Claudin-1 expression
in vivo.
doi:10.1016/j.mod.2009.06.155
03-P103
Morphogenesis and cellular mechanisms in mice molar root
development
Min-A. Choi1, Wern-Joo Sohn2, Hye-In Jung3, Hong-In Shin1,
Sang Gyu Lee2, Han-Sung Jung4, Jae-Young Kim3
1 Department of Oral Pathology and Regenerative Medicine, School of
Dentistry, IHBR, Kyungpook National University, Daegu, Republic of
Korea2 School of Life Science and Biotechnology, Kyungpook National Univer-
sity, Daegu, Republic of Korea3 Department of Biochemistry, School of Dentistry, IHBR, Kyungpook
National University, Daegu, Republic of Korea4 Division in Anatomy and Developmental Biology, Department of Oral
Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain
Korea 21 project, Oral Science Research Center, College of Dentistry, Yo,
Seoul, Republic of Korea
Most of studies in tooth development have been focused on
the early development with crown formation. In root formation,
known as a following event after the completion of crown forma-
tion, developmental mechanisms and cellular events were not
elucidated properly. In this study, morphogenesis and cellular
mechanisms such as cytoskeletal formation, cell proliferation
and apoptosis have been examined with three dimensional
approaches during mouse root development. Specific localization
and expression patterns of cellular markers and signalling mole-
cules in the root forming region would suggest that differential
cellular mechanisms would be involved in the formation of root
and diaphragm regions in multi-rooted tooth development. Espe-
cially, cell proliferation and actinfilament formation, examined by
Ki67 immunostaining and phalloidin staining, showed the signif-
icant differences of localization patterns in root forming and dia-
phragm forming regions. In order to evaluate the precise cellular
mechanisms in root formation, we treated Cytochalasin D and
Nocodazole, well known inhibitors for actinfilament and microtu-
bule formations, during in vitro organ culture. Based on these
results, we could conclude that differential roles of cytoskeletal
formation and proliferation between root and diaphragm forming
region would play important roles in multi-rooted tooth
morphogenesis.
doi:10.1016/j.mod.2009.06.156
03-P104
Transient innervation of retinal axons to the deeper laminae
prior to establishment of the lamina-specific projection in chick
optic tectum
Minoru Omi1, Hidekiyo Harada2, Harukazu Nakamura1,2
1 Graduate School Life Science, Tohoku University, Sendai, Japan2 IDAC, Tohoku University, Sendai, Japan
The visual center of the lower vertebrates is the optic tectum.
Retinal fibers invade the tectum from the rostral, run through the
superficial layer of the tectum, make a right turn near the termi-
nal, and make terminal arborizations in the specific retinorecipi-
ent laminae. Previous studies have shown that the terminals
arborize only in upper laminae (above lamina g in SGFS) and it
has been accepted that retinal axons never enter deeper laminae.
We re-examined the projection pattern of retinal axons in the tec-
tum laminae of developing chick embryos utilizing high sensitive
tracing system in which gene of fluorescent protein marker is
integrated in the genome by transposon mediated system
S97M E C H A N I S M S O F D E V E L O P M E N T 1 2 6 ( 2 0 0 9 ) S 6 7 – S 1 0 6