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1 2 3 4 5 6 7 8 9 10 11 M A 0.3 0.6 0.1 0.7 0.5 M 1 2 3 4 5 6 7 8 9 10 11 B 0.3 0.6 0.1 0.7 0.5 34 Online Resource Fig. 1. Partial amplified ribosomal DNA restriction analysis (ARDRA) profiles of 11 species from Lighvan and Koozeh cheeses from different manufacturing and ripening stages. The 16S rRNA gene was amplified using primers 27F and 1492R and digested with the restriction enzymes HaeIII (A) and HhaI (B). M, molecular weight marker GeneRuler TM . After partial amplification, sequencing and sequence comparison of 16S rRNA genes, the profiles were shown to correspond to the following species: 1, Enterococcus faecium; 2, Lactobacillus plantarum; 3, Lactobacillus brevis; 4, Lactococcus lactis subsp. lactis; 5, Enterococcus faecalis; 6, Enterococcus durans; 7, Enterococcus casseliﬂavus; 8, Enterococcus italicus; 9, Micrococcus luteus; 10, Staphylococcus haemolyticus; and 11, Aerococcus viridans.

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M. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. A. M. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 0.7. 0.6. 0.5. 0.3. 0.1. B. 0.7. 0.6. 0.5. 0.3. 0.1. - PowerPoint PPT Presentation

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Online Resource Fig. 1. Partial amplified ribosomal DNA restriction analysis (ARDRA) profiles of 11 species from Lighvan and Koozeh cheeses from different manufacturing and ripening stages. The 16S rRNA gene was amplified using primers 27F and 1492R and digested with the restriction enzymes HaeIII (A) and HhaI (B). M, molecular weight marker GeneRulerTM. After partial amplification, sequencing and sequence comparison of 16S rRNA genes, the profiles were shown to correspond to the following species: 1, Enterococcus faecium; 2, Lactobacillus plantarum; 3, Lactobacillus brevis; 4, Lactococcus lactis subsp. lactis; 5, Enterococcus faecalis; 6, Enterococcus durans; 7, Enterococcus casseliflavus; 8, Enterococcus italicus; 9, Micrococcus luteus; 10, Staphylococcus haemolyticus; and 11, Aerococcus viridans.

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