1 Cellular Division 2 Cell Division All cells are derived from pre- existing cells All cells are derived from pre- existing cells New cells are produced for growth and to replace damaged or old cells New cells are produced for growth and to replace damaged or old cells Differs in prokaryotes (bacteria) and eukaryotes (protists, fungi, plants, & animals) Differs in prokaryotes (bacteria) and eukaryotes (protists, fungi, plants, & animals) 3 Keeping Cells Identical The instructions for making cell parts are encoded in the DNA, so each new cell must get a complete set of the DNA molecules 4 DNA Replication DNA must be copied or replicated before cell division DNA must be copied or replicated before cell division Each new cell will then have an identical copy of the DNA Each new cell will then have an identical copy of the DNA Original DNA strand Two new, identical DNA strands 5 Prokaryotic Chromosome The DNA of prokaryotes (bacteria) is one, circular chromosome attached to the inside of the cell membrane The DNA of prokaryotes (bacteria) is one, circular chromosome attached to the inside of the cell membrane 6 Eukaryotic Chromosomes All eukaryotic cells store genetic information in chromosomes All eukaryotic cells store genetic information in chromosomes Most eukaryotes have between 10 and 50 chromosomes in their body cells Most eukaryotes have between 10 and 50 chromosomes in their body cells Human body cells have 46 chromosomes or 23 identical pairs Human body cells have 46 chromosomes or 23 identical pairs 7 Eukaryotic Chromosomes Each chromosome is composed of a single, tightly coiled DNA molecule Each chromosome is composed of a single, tightly coiled DNA molecule Chromosomes cant be seen when cells arent dividing and are called chromatin Chromosomes cant be seen when cells arent dividing and are called chromatin 8 Compacting DNA into Chromosomes DNA is tightly coiled around proteins called histones 9 Chromosomes in Dividing Cells Duplicated chromosomes are called chromatids & are held together by the centromere Called Sister Chromatids 10 Karyotype A picture of the chromosomes from a human cell arranged in pairs by size A picture of the chromosomes from a human cell arranged in pairs by size First 22 pairs are called autosomes First 22 pairs are called autosomes Last pair are the sex chromosomes Last pair are the sex chromosomes XX female or XY male XX female or XY male 11 Cell Reproduction 12 Types of Cell Reproduction Asexual reproduction involves a single cell dividing to make 2 new, identical daughter cells Asexual reproduction involves a single cell dividing to make 2 new, identical daughter cells Mitosis & binary fission are examples of asexual reproduction Mitosis & binary fission are examples of asexual reproduction Sexual reproduction involves two cells (egg & sperm) joining to make a new cell (zygote) that is NOT identical to the original cells Sexual reproduction involves two cells (egg & sperm) joining to make a new cell (zygote) that is NOT identical to the original cells Meiosis is an example Meiosis is an example 13 Cell Division in Prokaryotes 14 Cell Division in Prokaryotes Prokaryotes such as bacteria divide into 2 identical cells by the process of binary fission Prokaryotes such as bacteria divide into 2 identical cells by the process of binary fission Single chromosome makes a copy of itself Single chromosome makes a copy of itself Cell wall forms between the chromosomes dividing the cell Cell wall forms between the chromosomes dividing the cell Parent cell 2 identical daughter cells Chromosome relicates Cell splits 15 The Cell Cycle 16 Five Phases of the Cell Cycle G 1 - primary growth phase G 1 - primary growth phase S synthesis; DNA replicated S synthesis; DNA replicated G 2 - secondary growth phase G 2 - secondary growth phase collectively these 3 stages are called interphase M - mitosis M - mitosis C - cytokinesis C - cytokinesis 17 Cell Cycle 18 Whats Happening in Interphase? What the cell looks like Animal Cell Whats occurring 19 Sketch the Cell Cycle Daughter Cells DNA Copied Cells Mature Cells prepare for Division Cell Divides into Identical cells 20 Mitosis 21 Mitosis Division of the nucleus Division of the nucleus Also called karyokinesis Also called karyokinesis Only occurs in eukaryotes Only occurs in eukaryotes Has four stages Has four stages Doesnt occur in some cells such as brain cells Doesnt occur in some cells such as brain cells 22 Four Mitotic Stages Prophase Prophase Metaphase Metaphase Anaphase Anaphase Telophase Telophase 23 Spindle Fiber attached to Chromosome Kinetochore Fiber Chromosome 24 Review of Prophase What the cell looks like Whats happening 25 Spindle Fibers The mitotic spindle form from the microtubules in plants and centrioles in animal cells The mitotic spindle form from the microtubules in plants and centrioles in animal cells Polar fibers extend from one pole of the cell to the opposite pole Polar fibers extend from one pole of the cell to the opposite pole Kinetochore fibers extend from the pole to the centromere of the chromosome to which they attach Kinetochore fibers extend from the pole to the centromere of the chromosome to which they attach Asters are short fibers radiating from centrioles Asters are short fibers radiating from centrioles 26 Sketch The Spindle 27 Metaphase Chromosomes, attached to the kinetochore fibers, move to the center of the cell Chromosomes, attached to the kinetochore fibers, move to the center of the cell Chromosomes are now lined up at the equator Chromosomes are now lined up at the equator Pole of the Cell Equator of Cell 28 Metaphase Chromosomes lined at the Equator Asters at the poles Spindle Fibers 29 Review of Metaphase What the cell looks like Whats occurring 30 Anaphase Occurs rapidly Occurs rapidly Sister chromatids are pulled apart to opposite poles of the cell by kinetochore fibers Sister chromatids are pulled apart to opposite poles of the cell by kinetochore fibers 31 Anaphase Sister Chromatids being separated 32 Anaphase Review What the cell looks like Whats occurring 33 Telophase Sister chromatids at opposite poles Sister chromatids at opposite poles Spindle disassembles Spindle disassembles Nuclear envelope forms around each set of sister chromatids Nuclear envelope forms around each set of sister chromatids Nucleolus reappears Nucleolus reappears CYTOKINESIS occurs CYTOKINESIS occurs Chromosomes reappear as chromatin Chromosomes reappear as chromatin 34 Comparison of Anaphase & Telophase 35 Cytokinesis Means division of the cytoplasm Means division of the cytoplasm Division of cell into two, identical halves called daughter cells Division of cell into two, identical halves called daughter cells In plant cells, cell plate forms at the equator to divide cell In plant cells, cell plate forms at the equator to divide cell In animal cells, cleavage furrow forms to split cell In animal cells, cleavage furrow forms to split cell 36 Cytokinesis Cleavage furrow in animal cell Cell plate in plant cell 37 Daughter Cells of Mitosis Have the same number of chromosomes as each other and as the parent cell from which they were formed Have the same number of chromosomes as each other and as the parent cell from which they were formed Identical to each other, but smaller than parent cell Identical to each other, but smaller than parent cell Must grow in size to become mature cells (G 1 of Interphase) Must grow in size to become mature cells (G 1 of Interphase) 38 Uncontrolled Mitosis If mitosis is not controlled, unlimited cell division occurs causing cancerous tumors If mitosis is not controlled, unlimited cell division occurs causing cancerous tumors Oncogenes are special proteins increase the chance that a normal cell develops into a tumor cell Oncogenes are special proteins that increase the chance that a normal cell develops into a tumor cell Cancer cells 39 Meiosis Formation of Gametes (Eggs & Sperm) 40 Facts About Meiosis Preceded by interphase which includes chromosome replication Preceded by interphase which includes chromosome replication Two meiotic divisions --- Meiosis I and Meiosis II Two meiotic divisions --- Meiosis I and Meiosis II Called Reduction- division Called Reduction- division Original cell is diploid (2n) Original cell is diploid (2n) Four daughter cells produced that are monoploid (1n) Four daughter cells produced that are monoploid (1n) 41 Facts About Meiosis Daughter cells contain half the number of chromosomes as the original cell Daughter cells contain half the number of chromosomes as the original cell Produces gametes (eggs & sperm) Produces gametes (eggs & sperm) Occurs in the testes in males (Spermatogenesis) Occurs in the testes in males (Spermatogenesis) Occurs in the ovaries in females (Oogenesis) Occurs in the ovaries in females (Oogenesis) 42 Start with 46 double stranded chromosomes (2n) Start with 46 double stranded chromosomes (2n) After 1 division - 23 double stranded chromosomes (n) After 1 division - 23 double stranded chromosomes (n) After 2nd division - 23 single stranded chromosomes (n) After 2nd division - 23 single stranded chromosomes (n) Occurs in our germ cells that produce gametes Occurs in our germ cells that produce gametes More Meiosis Facts 43 Why Do we Need Meiosis? It is the fundamental basis of sexual reproduction It is the fundamental basis of sexual reproduction Two haploid (1n) gametes are brought together through fertilization to form a diploid (2n) zygote Two haploid (1n) gametes are brought together through fertilization to form a diploid (2n) zygote 44 Fertilization Putting it all together 1n =3 2n = 6 45 Replication of Chromosomes Replication is the process of duplicating a chromosome Replication is the process of duplicating a chromosome Occurs prior to division Occurs prior to division Replicated copies are called sister chromatids Replicated copies are called sister chromatids Held together at centromere Held together at centromere Occurs in Interphase 46 A Replicated Chromosome Homologs Homologs (same genes, different alleles) (same genes, different alleles) Sister Chromatids (same genes, same alleles) Gene X Homologs separate in meiosis I and therefore different alleles separate. 47 Meiosis Forms Haploid Gametes Meiosis must reduce the chromosome number by half Meiosis must reduce the chromosome number by half Fertilization then restores the 2n number Fertilization then restores the 2n number from momfrom dadchild meiosis reduces genetic content too much! The right number! 48 Meiosis: Two Part Cell Division Homologsseparate Sisterchromatidsseparate Diploid Meiosis I Meiosis II Diploid Haploid 49 Meiosis I: Reduction Division Nucleus Spindlefibers Nuclearenvelope Early Prophase I (Chromosome number doubled) Late Prophase I Metaphase I Anaphase I Telophase I (diploid) 50 Prophase I Early prophase Homologs pair. Homologs pair. Crossing over occurs Crossing over occurs. Late prophase Chromosomes condense. Chromosomes condense. Spindle forms. Spindle forms. Nuclear envelope fragments. Nuclear envelope fragments. 51 Tetrads Form in Prophase I Homologous chromosomes (each with sister chromatids) Homologous chromosomes (each with sister chromatids) Join to form a TETRAD Called Synapsis 52 Crossing-Over Homologous chromosomes in a tetrad cross over each other Homologous chromosomes in a tetrad cross over each other Pieces of chromosomes or genes are exchanged Pieces of chromosomes or genes are exchanged Produces Genetic recombination in the offspring Produces Genetic recombination in the offspring 53 Homologous Chromosomes During Crossing-Over 54 Crossing-over multiplies the already huge number of different gamete types produced by independent assortment Crossing-Over 55 Metaphase I Homologous pairs of chromosomes align along the equator of the cell 56 Anaphase I Homologs separate and move to opposite poles. Sister chromatids remain attached at their centromeres attached at their centromeres. 57 Telophase I Nuclear envelopes reassemble. Spindle disappears. Cytokinesis divides cell into two. 58 Meiosis II Only one homolog of each chromosome is present in the cell Only one homolog of each chromosome is present in the cell. Meiosis II produces gametes with one copy of each chromosome and thus one copy of each gene. Sister chromatids carry identical genetic information. Gene X 59 Meiosis II: Reducing Chromosome Number Prophase II Metaphase II Anaphase II Telophase II 4 Identical haploid cells 60 Prophase II Nuclear envelope fragments. Spindle forms. 61 Metaphase II Chromosomes align along equator of cell. 62 Anaphase II Sister chromatids separate and move to opposite poles Sister chromatids separate and move to opposite poles. Equator Pole 63 Telophase II Nuclear envelope assembles. Chromosomes decondense. Spindle disappears. Cytokinesis divides cell into two. 64 Results of Meiosis Gametes (egg & sperm) form Four haploid cells with one copy of each chromosome One allele of each gene Different combinations of alleles for different genes along the chromosome 65 Gametogenesis Oogenesis or Spermatogenesis 66 Spermatogenesis Occurs in the testes Occurs in the testes Two divisions produce 4 spermatids Two divisions produce 4 spermatids Spermatids mature into sperm Spermatids mature into sperm Men produce about 250,000,000 sperm per day Men produce about 250,000,000 sperm per day 67 Spermatogenesis in the Testes Spermatid 68Spermatogenesis 69 Oogenesis Occurs in the ovaries Occurs in the ovaries Two divisions produce 3 polar bodies that die and 1 egg Two divisions produce 3 polar bodies that die and 1 egg Polar bodies die because of unequal division of cytoplasm Polar bodies die because of unequal division of cytoplasm Immature egg called oocyte Immature egg called oocyte Starting at puberty, one oocyte matures into an ovum (egg) every 28 days Starting at puberty, one oocyte matures into an ovum (egg) every 28 days 70 Oogenesis in the Ovaries 71 Oogenesis Oogonium(diploid) Mitosis Primaryoocyte(diploid) Meiosis I Secondaryoocyte(haploid) Meiosis II (if fertilization occurs) First polar body may divide (haploid)Polarbodiesdie Ovum (egg) Second polar body (haploid) a A X X a X A X a X a X Matureegg A X A X 72 Comparing Mitosis and Meiosis 73 MitosisMeiosis Number of divisions 12 Number of daughter cells 24 Genetically identical? YesNo Chromosome # Same as parent Half of parent Where Somatic cells Germ cells When Throughout life At sexual maturity Role Growth and repair Sexual reproduction Comparison of Divisions