11 20-09 mbsb single molecule techniques.ppt
TRANSCRIPT
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Image from: http://www.bnl.gov/rhic/news/102307/story3.asp
Images from: Hecht Optics 4th ed. (2002)
Image from: http://www.aanda.org
Image from: http://d-d-photographics.com
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Images from: Hecht Optics 4th ed. (2002)
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Image from: http://www.olympusmicro.com/primer/techniques/fluorescence/anatomy/ix70fluorescence.html
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(photon)
e-
current out
Image from: Zhang et. al. J. Phys. B: At. Mol. Opt. Phys. 42 (2009)
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Fluorescence OutImage from: Zheng-Xing et al. ACTA BIOCHIMICA et
BIOPHYSICA SINICA. 2003, 35(4): 381-386
Excitation In
Fluorescence Out
Excitation In
Pinhole
Image from: http://www.olympusfluoview.com/theory/confocalintro.html
Fluorescence Out
102 nm
10 um
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V
Image from: Smith et al Science. 243 4888 (1989)
Image from: Deutsch Science. 240 4854 (1988)
Image from: Bensimon et. al. Phys. Rev. Lett. 74 (1995)
Image from: Kim & Larson Nucleic Acids Research. (2007)
Image from: Kim & Larson Nucleic Acids Research. (2007)
Image from: Galletto et al. Nature. 443 (2006)
Image from: Galletto et al. Nature. 443 (2006)
Image from: Galletto et al. Nature. 443 (2006)
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Image from: Hein et al PNAS. 105 38 (2008)
Image from: Hein et al PNAS. 105 38 (2008)
Confocal STEDIn vivo stained ER
Image from: Hein et al PNAS. 105 38 (2008)
Image from: Greenfield et al PLoS Biology. (2009)
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• QE of > 0.1
• > 20,000 cm-1 M-1
Table from: Gell, Brockwell & Smith Handbook of Single Molecule Spectroscopy. (2006)
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Image from: Abe et al Biochemistry. 42:51 (2003)
Image from: English et al Nat. Chem. Biol. 2:2 (2006)
Image from: English et al Nat. Chem. Biol. 2:2 (2006)
Image from: Sase et al PNAS. 94 (1997)
Image from: Sase et al PNAS. 94 (1997)
Image from: Yildiz et al Science. 27 (2003)
Image from: Yildiz et al Science. 27 (2003)
Image from: Yildiz et al Science. 27 (2003)
Image from: Liu et al Science. 322 (2008)
Image from: Liu et al Science. 322 (2008)
Image adapted from: Shirude & Balasubramanian Biochemie. 90:8 (2008)
Image from: Shirude & Balasubramanian Biochemie. 90:8 (2008)
Image from: PDB entry 3CRX Image from: Ha Biochemistry. 43 (2004)
Image from: Ha Biochemistry. 43 (2004)
Image from: http://www.aist.go.jp/
2nm 4nm
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Image from: Chen et al Biophys J. 92 (2007)
Image from: Capitanio et al. J. Opt. A: Pure Appl. Opt. 9 (2007)
Image from: Sönnichsen Plasmons in metal nanostructures. (2001)
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Image from: Sönnichsen et. al. Nature Biotechnology. 23 (2005)
Image from: Reinhard et. al. PNAS. 104:8 (2006)
Image from: Jun et. al. PNAS. 106:42 (2009)
Image from:Image from: Jun et. al. PNAS. 106:42 (2009)
