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2012-iGEM Break Symmetry Southeast university

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20 12-iGEM. Southeast university. Break Symmetry. 程 鹏. 周 乐. 周醒驭. 徐小丹. Team members. 凌昕彤. 桂 凯. 陈牧原. 张 俊. 樊雪龙. 高 珅. 贾正阳. Technical Background. Design Scheme. Contents. Experiment. Results && Biobricks &&Potential Work. Biosafety &&Human Practice. - PowerPoint PPT Presentation

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Page 1: 20 12-iGEM

2012-iGEMBreak Symmetry

Southeast university

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凌昕彤

周醒驭

陈牧原

高 珅

Team members

周 乐 程 鹏

桂 凯

张 俊 樊雪龙

徐小丹

贾正阳

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Technical Background

Design Scheme

Results && Biobricks &&Potential Work

ContentsContents

Biosafety &&Human Practice

Experiment

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Technical BackgroundTechnical Background

Create a star!

Main GoalMain Goal : Engineering bacteria colony to automatically break their own symmetry

: Engineering bacteria colony to automatically break their own symmetry

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Whole System

AHL transduction

Light sensor

Toggle switch

Toggle switch

Antisense FtsZ repress division

Antisense FtsZ repress division

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FtsZ mRNA

FtsZ asRNA

FtsZ geneFtsZ protein

silencing

transcription

translate

Tby gene transcription

Lux I gene Lux I protein

SAM

AHL

luxR gene express luxR protein

combine

Luxpr promoterpromote

pcI promoter

Lac promoter

lacI promoter

lacI gene

cI gene cI protein

lacI proteinpromote

promoteinhibit

inhibit

IPTG

ultraviolet rays

promote

Light sensor promoter

cI gene cI proteinpromote

Light receiverexpress

Red light

OmpC OmpC

express

express

express

express

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Light Sensor

Red Light11

Blue Light22

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Red Light

Light Sensor

Cph8Cph8

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RegulatoryBLp

ycgZ ycgF ycgE

regulated by the ycgF/E system

Blue Light

Conformation

Binding&&Repress Expression

Blue Light

Light Sensor

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Toggle Switch

Toggle Switch

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lacI+pL LuxR LuxIGenerating More AHL

Activating

AHL-Based Quorum Sensing

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Antisense RNA

Antisense RNA

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Verification for Antisense RNA

IPTG=0

After 10 hours in 37°C environment

IPTG=100 IPTG=200

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Design Scheme

Related Kits:

j5526=r11+b34+mRFP+tt i13507=b34+mRFP+tt i13521=tetR+b34+mRFP+tt j04450=lac+b34+mRFP+tt

r0011=lacipl r0051=pci r0040=ptetr r0063=luxpl(ahl--) r0062=luxpr(ahl++) r1051=r0051=pcI

i732820=b34+LacI+tt j23039=tetR+b34+luxI+tt i13973=pcI+b34+yfp+tt k098991=pcI+b34+gfp+tt p0451=b34+cI+tt k092400=b34+luxi+tt i13202=lacipl+b34+luxI+tt

t9002=tetr+b34+luxr+tt+luxpr+b32+gfp+tt f2620=ptetr+b34+luxr+tt+luxpr

k145270=pro+b34+luxr+tt j37033=b34+luxr k091204=pro+b34+luxr+tt i0462=b34+luxr+tt k116640=ptetR+b32+luxr+tt j37015=ptetR+b34+luxr+tt+luxpr+b34+luxI+b34+gfp+tt

K088006=b33+lacI+lac+b34+cI+b32+gfp+tt+pcI+b34+rfp+tt

I763026=ptetR+b34+lacI+tt I763035=ptetr+b34+lacI+b34+gfp+tt p0151=b31+cI+tt p0440=b34+tetr+tt

k145205=ptetr+b32+gfp+tt K082034=lac+b34+gfp+tt

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The Simulation Experiment

The Micro-Model

-Based on Cellular Automaton

The Macro-Model

-Based on Improved Cellular

Automaton

Modeling

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Approximate Bayesian Computation ( ABC ) for parameter inference in gene expression model

Modeling

1. Approximate Bayesian computation (ABC)

ABC rejection sampler

ABC MCMC

2. Gene expression model

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Modeling

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Results

Results

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Verification for Antisense RNA

IPTG=0

After 10 hours in 37°C environment

IPTG=100 IPTG=200

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others

Potential applications

Biosensor

Cell Printing

Signal Transnduction

Potential applications

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Acknowledgements

We would like to thank :Academy of Biomedical Engineering for all these support;

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数学模型数学模型

文档文档

反义反义

光敏启动光敏启动

开关开关

AHLAHLOthers

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该项目大部分由我们自主完成(除了非天然序列发夹结构外包合成),技术上不依赖外援。

项目总结 由于缺乏经验,该项目实现效果不如我们预期,但已部分实现。

下一步我们将完成元件的标准化和功能验证,以及各种材料的整理和项目展示工作。