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IOWA STATE UNIVERSITY

OF SCIENCE AND TECHNOLOGY

2011 IOWA EVALUATION OF INSECTICIDES AND

PLANT-INCORPORATED PROTECTANTS

CORN PESTS RESEARCH PROJECT

DEPARTMENT OF ENTOMOLOGY AMES, IOWA 50011-3140 SUE BLODGETT, CHAIR

INSECTS INVESTIGATED

Corn Rootworm European corn borer Corn Earworm

Report Authors April 10, 2012 Aaron Gassmann File number: 289-11 Patrick Weber

2011 IOWA EVALUATION OF INSECTICIDES AND PLANT INCORPORATED PROTECTANTS

TABLE OF CONTENTS

Page 1. Research Personnel .......................................... 1 2. Introduction and Objective ............................... 2 3. Testing Procedures and Evaluations ............... 2 4. Studies Conducted .......................................... 13 5. Corn Rootworm Evaluation Tables Ames Pioneer CMRA study Root Injury/Percent Consistency ........... 14 Stand Count .......................................... 14 Yield ...................................................... 15 Pioneer dual pyramid efficacy and yield for corn rootworm Stand Count .......................................... 15 Root Injury/Percent Consistency ........... 16 Yield ...................................................... 16 Monsanto corn rootworm study Root Injury/Percent Consistency ........... 17 Stand Count .......................................... 17 Yield ...................................................... 18 Monsanto genuity VT3P seed blend study Stand Count .......................................... 18 Yield ...................................................... 19

Table of Contents (Continued) Page Ames Monsanto smartstax features and benefits study Stand Count .......................................... 19 Yield ...................................................... 20 Ames (Old Dairy Farm) Syngenta experimental hybrid efficacy study Root Injury/Percent Consistency ........... 20 Ames (South Dairy Farm) Syngenta experimental hybrid efficacy study Root Injury/Percent Consistency ........... 21 Crawfordsville Yield study Root Injury/Percent Consistency ........... 21 Stand Count .......................................... 22 Yield ...................................................... 22 Monsanto smartstax refuge in bag study Stand Count .......................................... 23 Yield ...................................................... 23 Monsanto smartstax features and benefits study Stand Count .......................................... 24 Yield ...................................................... 24 Northeast Iowa Monsanto corn rootworm study Root Injury/Percent Consistency ........... 25 Stand Count .......................................... 25 Percent Lodging .................................... 26 Yield ...................................................... 26 Nashua Yield study Root Injury/Percent Consistency ........... 27 Stand Count .......................................... 27 Percent Lodging .................................... 28 Yield ...................................................... 28

Table of Contents (Continued) Page Monsanto corn rootworm study Root Injury/Percent Consistency ........... 29 Stand Count .......................................... 29 Percent Lodging .................................... 30 Yield ...................................................... 30 6. European Corn Borer Evaluation Tables Ames Pioneer Optimum AcreMax II for European corn borer Stand Count .......................................... 31 ECB Whorl Injury ................................... 31 ECB Tunneling (cm) .............................. 32 Yield ...................................................... 32 7. Corn Earworm Evaluation Tables Ames Dow AgroSciences smartstax vs. HXX & RR2 corn traits study Stand Count .......................................... 33 Percent Damaged Kernels .................... 33 Kernel Area Consumed (cm2) ................ 33 Total Damaged Ears ............................. 33 Monsanto smartstax features and benefits study Stand Count .......................................... 34 Percent Damaged Kernels .................... 34 Kernel Area Consumed (cm2) ................ 34 Total Damaged Ears ............................. 34 8. Appendix I - Agronomic Information ............. 35 9. Appendix II – Weather Data ............................ 41 10. Appendix III - Materials Tested ..................... 50

11. Appendix IV - Research Pictures ................. 54

2011 IOWA EVALUATION OF INSECTICIDES AND PLANT INCORPORATED PROTECTANTS

CORN PESTS RESEARCH PROJECT

DEPARTMENT OF ENTOMOLOGY

AMES, IOWA 50011-3140

Project Leader: Aaron Gassmann

Agricultural Specialist: Patrick Weber

Postdoctoral Research Associate: Jennifer Petzold-Maxwell Graduate Research Assistants: Mike Dunbar Ryan Keweshan Eric Clifton Amanda Hoffmann

Lab Research Assistant: Jeremy Bolles

Technical Assistants: Charlie Ethington James Terry Lee Koenigsfeld Phil Johnson Walter Wohlford-Wessels University Research Farm Superintendents: Kent Berns Ryan Rusk Kenneth Pecinovsky Kevin VanDee

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INTRODUCTION

The corn rootworm species, which includes the western corn rootworm Diabrotica virgifera virfigera and northern corn rootworm D. barberi, are the most damaging pests of corn Zea mays in the Unites States Corn Belt. Eggs are laid in the soil during the fall and hatch the following spring. Larval feeding on corn roots in June may diminish yield by reducing plant growth and drought tolerance and by imposing harvesting losses due to plant lodging. Adult emergence from the soil is underway by early July and continues through late summer. Additional crop losses can be caused by the beetles feeding on the female flowers (silks) and on soft doughy kernels. In Iowa, crop rotation, where it fits cropping practices, remains the preferred method of control. However, it is also economically feasible to protect corn roots with insecticides and plant-incorporated protectants (transgenic seedcorn that contains a gene from the naturally occurring soil bacterium Bacillus thuringiensis (Bt). In additional to corn rootworm, several above-ground lepidopteran pests feed on corn in Iowa. These include the European corn borer Ostrinia nubilalis, black cutworm Agrotis ipsilon, fall armyworm Spodoptera frugiperda, and corn earworm Helicoverpa zea. Over the last decade, Bt corn varieties have been made commercially available to protect corn from these pests.

OBJECTIVE

The goal of this research program is to serve Iowa agriculture by monitoring and evaluating the performance of registered commercial insecticides and

transgenic corn hybrids. To achieve this goal, we maintain a viable, proactive, progressive and scientifically sound product evaluation program.

TESTING PROCEDURES AND EVALUATIONS

Field Sites: Product efficacy study plots were established at six Iowa locations in 2011. Corn rootworm (CRW) research fields are continually maintained on University farms located at: Ames, Johnson Farm; Crawfordsville, S.E. Research and Demonstration Farm; Nashua, N.E. Research and Demonstration Farm; Sutherland, N.W. Research and Demonstration Farm and Lewis, S.W. Research and Demonstration Farm. Each research field is divided into two sections which annually alternate as test plot and late planted trap crop. The seed planted for the trap crop is a mixed maturity blend with a greater proportion of late-maturing varieties. This trap crop constitutes a favorable environment for adult female CRW late in the season when other fields are maturing. In addition, we conducted corn rootworm (CRW) research on two second year corn fields at two Ames locations (ISU- Old Dairy Farm and ISU South Dairy Farm). Also, we conducted corn rootworm (CRW) research on a continuous corn field (since 2003) in a private grower’s field in northeastern Iowa.

Table 1: Lists the research conducted at each location, target pest, and other general plot information.

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Corn Rootworm Studies

Field plot design: The experimental design in most studies was a randomized complete block. Treatments in the yield studies at both Crawfordsville (Tables 17-19) and Nashua (Tables 28-31) were paired rows 75-feet in length with four replications. Treatments for the Pioneer dual pyramid study for corn rootworm at Ames (Tables 5-7) were four rows wide with 40 feet per row with four replications. The Pioneer CMRA study (Tables 2-4) at Ames were eight row treatments that were 25 feet in length with six replications. The treatments in the Monsanto corn rootworm studies at Ames (Tables 8-10), northeast Iowa (Tables 24-27) and Nashua (Tables 32-35), were eight row treatments with 40 feet per row with four replications. The Monsanto genuity VT3P study in Ames (Tables 11-12) were eight row treatments with 100 feet per row with one replication. The Monsanto smartstax features and benefits study in Ames (Tables 13-14) were eight row treatments with 200 feet per row with one replication. Treatments for the Syngenta experimental hybrid efficacy studies at Ames-ISU Old Dairy Farm (Table 15) and Ames-ISU South Dairy Farm (Table 16) were four row wide treatments that were 20 feet in length with four replications. The Monsanto smartstax RIB study in Crawfordsville (Tables 20-21) were eight row treatments with 100 feet per row with one replication. The Monsanto smartstax features and benefits study in Crawfordsville (Tables 22-23) were eight row treatments with 100 feet per row with one replication.

Application techniques: Seeds were pre-bagged and planted with a four-row John Deere Max EmergeTM 7100 integral planter that had 30 inch row spacings. The standard planter fiberglass seed hoppers with attached “finger pickup mechanism,” were replaced with modified units. On the new units, the metal plate that covered the “fingers” had been replaced with a 7/8-inch, clear Plexiglas plate. Inserted through the Plexiglas was a small stainless steel cylinder. The cylinder was positioned to deliver seed to the “pickup fingers.” At the beginning of each replication pre-bagged seeds were dumped into the steel cylinder. At the beginning of each replication, a hydraulic motor (attached to the planter’s drive shaft) was activated to deliver seed immediately into the ground. At the end of each replication, this same hydraulic motor was activated to expel any unplanted seed.

Plant-incorporated protectants: Plant-incorporated protectants were evaluated in corn hybrids producing insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt). These included the YieldGard hybrids of Monsanto that produce the Bt toxin Cry3Bb1 and the Herculex hybrids of Pioneer and Dow AgroSciences that produce the binary Bt toxin Cry34/Cry35. Seed treatments: All the DeKalb hybrids evaluated in the yield studies (Crawfordsville, Tables 17-19; Nashua, Tables 28-31) were treated by the respective company with Poncho 500. All the Mycogen hybrids evaluated in the yield studies (Crawfordsville, Tables 17-19; Nashua, Tables 28-31) were treated by the respective company with Cruiser

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Extreme® 250. For the Pioneer dual pyramid study for corn rootworm, (Ames, Tables 5-7) all treatments were treated with Cruiser® 250 except one treatment of the pure stand of RR2 where the seeds were treated with Poncho 1250/VOTIVO. For the Pioneer CMRA study (Ames, Tables 2-4) for corn rootworms and Pioneer Optimum AcreMax II study (Ames, Tables 36-39) for European corn borer all hybrids were treated with Cruiser® 250. For the blended refuge seeds (RR2) planted in the Pioneer dual pyramid study for corn rootworm all seeds were commercially treated with Poncho 1250/VOTIVO. In the Pioneer Optimum AcreMax II study for European corn borer all blended refuge seeds (RR2) planted were treated with Cruiser® 250 insecticidal seed treatment. For the Monsanto studies at Nashua (Tables 32-35), Ames (Tables 8-10) and northeast Iowa (Tables 24-27), Monsanto Genuity VT3P study in Ames (Tables 11-12), Monsanto smartstax features and benefits study at Ames (Tables 13-14) and Crawfordsville (Tables 22-23), and Monsanto smartstax RIB study in Crawfordsville (Tables 20-21) the Poncho 250 seed treatment was commercially applied. The Syngenta experimental hybrid efficacy studies at Ames-Old Dairy Farm (Tables 15) and Ames-South Dairy Farm (Tables 17) these hybrids were treated with Cruiser Extreme® 250.

All these seed treatments target secondary soil-borne pests, such as wireworm and seedcorn maggot. For some treatments, plant-incorporated protectants were combined with conventional insecticides.

Granular application treatments: Force 3G granular insecticide was

applied to one treatment in the Pioneer dual pyramid study for corn rootworm study and six treatments in each of the Syngenta experimental hybrid efficacy studies at Ames-Old Dairy Farm and Ames-South Dairy Farm with modified Noble® metering units mounted on the planter. Aztec 2.1G granular insecticide was applied to two treatments in the Yield studies at both Crawfordsville and Nashua with modified Noble® metering units mounted on the planter. The Noble units were calibrated in the laboratory to accurately deliver material at a tractor speed of 4 mph. Plastic tubes directed the granular treatments to either a 7-inch band ahead of the closing wheels (T-band, All-Terrain Banders), or to the seed furrow, placing all the insecticide in-furrow (Furrow). Eleven-inch poly-bristle skirts were attached to the frame and the frame positioned so the bristle tips touched the ground. Each row was constantly monitored to ensure that insecticides were correctly applied at all times. Final incorporation was accomplished with drag chains mounted behind the closing wheels.

SmartBoxTM application treatments: SmartChoice 5G and Counter 20G, treatments were applied with modified SmartBoxTM metering units. These products were applied as ounces per 1000 row feet. The commercial SmartBoxTM were removed from their large-base containers and sandwiched between a flat metal plate on the bottom and a custom-made, threaded plastic cap on the top. The bottom plate had been fabricated so that it could slide in and out of the same planter mounting brackets used for the Noble units. An inverted 1000 mL Nalgene bottle, screwed into the top cap provided a secure and sealed container for

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insecticide. A short plastic tube attached to the dispenser opening of the metering unit could be connected to either the planter’s T-band or Furrow tubes. The two controllers mounted in the tractor cab were used to operate the SmartBoxTM metering units. All treatments were applied at 4 mph using the “fixed speed mode” on the SmartBoxTM controllers.

European Corn Borer Study

Field plot design: The experimental design in the Pioneer Optinum AcreMax II study for European corn borer was a randomized complete block with four replications respectively. Five treatments were evaluated (Pioneer HX1 alone, Pioneer RR2 hybrid alone, Pioneer HX1 x YGCB (pyramid) alone, and with a 5% and 10% blended refuge seeds (RR2). These treatments were four rows wide, 40-feet in length. Plots were cut down to 38 feet of row for alley purposes. In the blended refuges, a gene check was used to identify refuge plants (rows 1 and 4: blended refuge treatments only). A total of twenty four three-plant clusters were marked with stakes for rows 1 and 4 of the 5% blended refuge treatment and the 10% blended refuge treatment. Two six-plant clusters were marked with stakes within rows 1 and 4 for the treatments of HX1 hybrid alone, HX1 x YGCB (pyramid) and RR2 hybrid alone for all four replications. Within the blended refuge treatments the plant clusters consisted of either: Refuge-Bt-Bt or Bt-Bt-Refuge.

Application techniques: The ECB neonates were obtained from the USDA-ARS Corn Insects and Crop Genetics Research Laboratory. We

obtained four bottles of neonates that were mixed with corn grit. We infested with first-generation ECB on 17 June and 24 June. The inoculum was applied into the whirl. When infesting with second-generation ECB on 9 August and 16 August, the inoculum was applied into the ear-leaf collar. Inoculum was equal to 100 ECB per plant and administered with bazooka inoculators.

On 15 July, the ECB injury was scored. A total of 288 plants were evaluated. We used the Pioneer 9-1 rating scale for evaluating first-generation ECB whorl injury.

On 6 October and 7 October, tunneling was evaluated by splitting stalks into three parts (top, shank, and bottom) with a curved linoleum knife. All three plant clusters in the two blended refuge treatments and all six plant clusters in the three pure stand treatments were evaluated for a total of 288 plants. Total tunnel length (cm) was scored for each plant part and averaged for each treatment.

Corn Earworm Study

Field plot design: The experimental design in this Dow AgroSciences smartstax vs Herculex XTRA and RR2 corn study in Ames was a randomized complete block with four replications. The treatments consisted of Mycogen RR2 corn, Mycogen smartstax and Mycogen Herculex XTRA. These treatments were four rows wide, 20-feet in length.

Application techniques: The 1st CEW instar larvae were obtained from Benzon Research from Carlisle, Pennsylvania. Prior to infestation, corn ear shoot bags were placed over the top ear shoot of all

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plants in row 2 and 3 of each plot. On 4 August a #2 camel hair brush was used to infest 10 ears per row (of rows 2 and 3) to the silks with 5, 1st instar larvae. One summer Technical Assistant was assigned one replication of treatments to infest.

On 18 August (14 days after infestation) 10 randomly selected ears were sampled from the two center rows of each plot. From these ears we counted the numbers of kernels damaged, in addition, we measured with a caliper (in millimeters) the average kernel cap size, both length and width. In addition, we counted the number of rows of kernels around the ear and the number of kernels in an average row length. From these numbers collected, we calculated the percentage of damaged kernels per ear and per treatment.

Corn Earworm Study

Field plot design: The experimental design in this Monsanto smartstax features & benefits study was a randomized complete block with four replications. The treatments consisted of Pioneer HXX (Pioneer 35F34PDR), Syngenta Agrisure (N53C-3111), DeKalb smartstax (DKC 55-09), DeKalb RR2 (DKC 55-08) with Aztec 2.1G 0.12 ounces ai/1000 rowft, Syngenta GT Agrisure (N53-W2) with Aztec 2.1G 0.12 ounces ai/1000 rowft, Pioneer RR2 (35F37) with Aztec 2.1G 0.12 ounces ai/1000 rowft and DeKalb RR2 (DKC 55-08) These treatments were eight rows wide, 200-feet in length.

Application techniques: The 1st CEW instar larvae were obtained from Benzon Research from Carlisle, Pennsylvania. Prior to infestation, corn ear shoot bags

were placed over the top ear shoot of 10 plants in row 2 and 7 of each plot. On 10 August a #2 camel hair brush was used to infest 10 ears per row (of rows 2 and 7) to the silks with 5, 2nd instar larvae. One summer Technical Assistant was assigned one replication of treatments to infest.

On 24 August (14 days after infestation) 10 randomly selected ears were sampled total from rows 2 and 7 of each plot. From these ears we counted the numbers of kernels damaged, in addition, we measured with a caliper (in millimeters) the average kernel cap size, both length and width. In addition, we counted the number of rows of kernels around the ear and the number of kernels in an average row length. From these numbers collected, we calculated the percentage of damaged kernels per ear and per treatment.

Corn Rootworm Larval

Evaluations

Stand counts: The number of plants in either 17.5, or 20 row-ft was recorded (note distance in foot notes of stand count tables). These were taken early and late in the growing season. The number of plants in 38 row-ft was recorded for both the Pioneer Dual Pyramid study for corn rootworm and Pioneer Optimum AcreMax II study for European corn borer. These were taken early and at Physiological maturity during the growing season. Root-Injury: Since CRW beetle emergence was delayed by 10-14 days, we delayed starting root digging until the first of the month of August. After the majority of corn rootworms had finished feeding, roots were dug from 8/01/2011

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through 8/17/2011. Five root systems total were dug per replication from each insecticide treatment (except from the My-95%smartstax/5%non-Bt treatment (refuge in the bag)) where nine root systems (6 smartstax + 3 non-Bt) total were dug per replication from the two-row yield studies (Crawfordsville, Table 17 & Nashua, Table 28). For the Monsanto corn rootworm studies at Nashua (Table 32), Ames (Table 8) and northeast Iowa (Table 24), five root systems total were dug from each insecticide treatment out of rows 2 and 7. With the Pioneer CMRA study, six roots total were dug per treatment from rows two and seven (Ames, Table 3). With the Pioneer dual pyramid study for corn rootworm, we dug 48 root systems per treatment from the four pure stand treatments (HXX + MIR604, RR2 Hybrid + Force 3G at 4 oz/1000feet of row, RR2 hybrid with Poncho 1250/VOTIVO insecticidal seed treatment, RR2 hybrid with Cruiser 250 insecticidal seed treatment. 72 root systems per treatments were dug from the two blended refuge treatments (HXX + MIR604 (pyramid), with 5% blended refuge seeds-P0413R, HXX + MIR604 (pyramid) with 10% blended refuge seeds-P0413R). Roots were dug out of rows 1 and 4 only. With the Syngenta experimental hybrid efficacy study, a total of six root systems total were dug from rows one and four of each treatment (Ames-Old Dairy Farm- (Table 15) and Ames-South Dairy Farm-(Table 16). Prior to leaving the field, the roots were marked with a permanent marker with the plot number, study name and location. Excess soil was removed in the field as well. In Ames, roots were soaked in water and then a pressurized water spray was used to remove the

remaining soil. Roots were then laid out by replications and were then evaluated for rootworm feeding injury on the following Iowa State Node-Injury Scale (0-3): Node-Injury Scale (0-3): 0.00 - No feeding damage (lowest rating

that can be given). 1.00 - One node (circle of roots), or the

equivalent of an entire node, eaten back to with-in ~ 1½ inches of the stalk (soil line on the 7th node).

2.00 - Two nodes eaten.

3.00 - Three or more nodes eaten. (Highest rating that can be given)

Damage in-between complete nodes eaten is noted as the percentage of the node missing, i.e. 1.50 = 1½ nodes eaten, 0.25 = ¼ of one node eaten, and so on.

The linear node-injury scale allows injury to be expressed intuitively and has proved useful in evaluating minor injury, especially with transgenic seed corn. For an interactive guide to the Node-injury scale, see the Iowa State University Entomology web site at:

rootworm/nodeinjury/nodeinjury.html

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Product consistency: The percent product consistency was also calculated for each treatment. Product consistency equals the percentage of times a treatment limited feeding injury to 0.25 node or less (greater injury can result in economic yield loss, especially when plants are moisture stressed).

Lodging counts: A plant was considered lodged if it was leaning at least 30 degrees from vertical. These were taken at harvest time along with final stand count numbers (note distance in footnotes of lodging count tables).

Yields: Studies that were taken to yield were machine harvested with a modified John Deere 9410 plot combine or John Deere 9450. Weights (pounds) and percent moisture were recorded from either a HarvestMaster brand or Shivvers brand 5010 moisture meter plot harvest data collection system. These measurements were converted to bushels per acre of No. 2 shelled corn (56 lbs. per bushel) at 15% moisture in an Excel worksheet.

All data were analyzed with standard ANOVA procedures using SAS Enterprise Guide 4.2. When a significant treatment effect was present pairwise comparisons made among

means with an experiment wise error rate of P < 0.05.

COMMENTS ON INSECTICIDES AND PLANT INCORPORATED

PROTECTANTS (PIP) PERFORMANCE

Tables list treatment rates as ounces active ingredient per 1000 row foot unless otherwise indicated in the footnotes.

CORN ROOTWORM EVALUATIONS

AMES (Johnson farm) Pioneer CMRA study (Tables 2-4): Both the Herculex XTRA treatments (with and without Bio-insecticide) had significantly less node-injury than the two Herculex 1 treatments (Table 2). The Herculex 1 alone treatment had a significantly lower stand count than the other three treatments (Table 3). No significant differences in yield were detected (Table 4). Pioneer dual pyramid yield and efficacy for corn rootworm (Tables 5-7): No significant differences were noted for stand counts (Table 5). Root injury was lowest and product consistency greatest for RR2 with Force 3G. Product consistency was lowest for RR2 in the 10% blended refuge treatment. In general the pyramid had lower root injury and higher product consistency than RR2, except for RR2 with Force 3G (Table 6). The pure stand of RR2 with Poncho 1250/VOTIVO insecticidal seed treatment was the highest yielding and was significantly greater than the pure stand of RR2 with the Cruiser250 insecticidal seed treatment and the pyramid with a 10% blended refuge. (Table 7).

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AMES (Johnson farm) Monsanto corn rootworm study (Tables 8-10): The Mycogen Herculex XTRA treatment was the same as the DeKalb RR2 hybrid treatment but was significantly different than all other treatments for node-injury (Table 8). Regarding product consistency, the Mycogen Herculex XTRA treatment was the same as the smartstax treatment but both were significantly different than the remainder of the treatments (Table 8). With stand counts all treatments were the same except the DeKalb YieldGard VT3 + Force overtop and DeKalb RR2 hybrid which were significantly different from each other (Table 9). No significant differences in yield were detected (Table 10). Monsanto genuity VT3P study (Tables 11-12): No significant differences were noted for stand counts (Table 11). No significant differences in yield were detected (Table 12). Monsanto smartstax features and benefits study (Tables 13-14): No significant differences were noted for stand counts (Table 13). No significant differences in yield were detected (Table 14). AMES (Old Dairy Farm) Syngenta experimental hybrid efficacy study (Tables 15): Rootworm pressure was low but we did see a significant difference with the Non-Bt hybrid showing greater node-injury compared to the other treatments (Table 15).

AMES (South Dairy Farm) Syngenta experimental hybrid efficacy study (Tables 16): Rootworm pressure was low but we did see a significant difference with the Non-Bt hybrid showing greater node-injury compared to the other treatments (Table 16). CRAWFORDSVILLE (S.E. R&D FARM) Yield study (Tables 17-19): Node injury was significantly higher for the two isoline treatments (checks) than all other treatments. The treatment of Mycogen Herculex HXX with SmartChoice-SB insecticide In-furrow over top was the same as the DeKalb Smartstax with Aztec 2.1G overtop treatment and the DeKalb Smartstax with Aztec 2.1G overtop was the same as Mycogen HXX plus Force 3G overtop and the Mycogen Smartstax treatments (Table 17). Regarding stand counts, the Mycogen Isoline hybrid was the same as all treatments except the Mycogen 95% Smartstax/5%Non-Bt which was significantly different (Table 18). With yield the Mycogen Isoline hybrid was the same as all other treatments except the Mycogen HXX with Force 3G and SmartChoice 5G SmartBox applied In-furrow overtop (Table 19). Monsanto smartstax RIB study (Tables 20-21): No significant differences were noted for stand counts (Table 20). No significant differences in yield were detected either (Table 21). Monsanto smartstax features and benefits study (Tables 22-23): The Agrisure hybrid (Agrisure 3111) treatment was significantly different than all other treatments for stand counts

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(Table 22). No significant differences in yield were detected (Table 23). Northeast Iowa Monsanto corn rootworm study (Tables 24-27): There was heavy CRW pressure at this site. The DeKalb Isoline Hybrid (DKC 44-92) treatment had the highest node-injury followed by the DeKalb YieldGard VT3 hybrid (DKC 46-60) followed by the Mycogen Herculex Hybrid (DKC 2K592) treatment and finally the DeKalb Isoline Hybrid (DKC 44-92) treatment with Force overtop. These four treatments were significantly different than all the other treatments (Table 24). No significant differences were noted for stand counts (Table 25). The DeKalb Isoline Hybrid (DKC 44-92) and the DeKalb YieldGard VT3 hybrid (DKC 46-60) treatments had significantly greater percent lodging than all other treatments (Table 26). No significant differences in yield were detected (Table 27). NASHUA (N.E. R&D FARM) Yield study (Tables 28-31): The treatment of DeKalb-Smartstax with Aztec 2.1G overtop In-furrow had significantly less node-injury than all ten other treatments (Table 28). No significant differences among stand counts were noted (Table 29). Regarding percent lodging, the DeKalb Isoline and Mycogen Isoline hybrid treatments were significantly different than all other treatments (Table 30). For yield, the DeKalb Isoline and Mycogen Isoline hybrid treatments were significantly different than all other treatments (Table 31). Monsanto corn rootworm study (Tables 32-35): The smartstax hybrid

(DKC 46-61) treatment had significantly less node-injury than all other treatments. Both the YieldGard VT3 hybrid (DKC 46-60) treatment and DeKalb Isoline (DKC 61-72) treatment with either Aztec or Force overtop had significant less node-injury than either one of these hybrids alone (Table 32). The smartstax hybrid (DKC 46-61) treatment had significantly lower stand count compared to the Mycogen Herculex hybrid (DKC 2K592) and DeKalb Isoline hybrid (DKC 61-72) plus Force applied overtop treatments (Table 33). The DeKalb Isoline Hybrid (DKC 61-72) treatment had significantly greater percent lodging than all other treatments (Table 34). For yields, the Mycogen Herculex Hybrid (DKC 2K592) treatment was significantly greater than all other treatments in this study (Table 35).

EUROPEAN CORN BORER EVALUATIONS

AMES (Johnson farm) Pioneer Optimum AcreMAX II study for European corn borer (Table 36-39): Stand counts were similar among all treatments (Table 36). With whorl injury, the RR2 plants within the 10% blended refuge were significantly greater than the other treatments (Table 37). Tunneling of ECB was significantly lower on HX1 and the pyramid (HX1 x YGCB) than on RR2 either alone or in a blended refuge (Table 38). No significant differences in yield were detected (Table 39).

CORN EARWORM EVALUATIONS AMES (Johnson farm) Dow Agro Sciences Smartstax vs. Herculex XTRA and RR2 corn traits

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study (Tables 40-42): The HXX/RR2 treatment had significantly lower stand count than the RR2 but was similar to the smartstax treatment (Table 40). Regarding damaged kernels per ear, percent damaged per ear, kernel area consumed (cm) and total damaged ears both the RR2 and HXX/RR2 treatments were significantly higher than the smartstax treatment (Tables 41-42). AMES (Johnson farm) Monsanto smartstax features and benefits study (Tables 43-44): Regarding damaged kernels per ear, percent damaged per ear, kernel area consumed (cm) and total damaged ears, both the Agrisure hybrid (Agrisure 3111) and smartstax (DKC 55-09) treatments were significantly lower than all other treatments (Tables 43-44).

CALIBRATION INFORMATION

All Noble® units were laboratory calibrated and units were randomly spot-checked in the field prior to planting. SmartBoxTM units were calibrated on the planter in accordance with the SmartBox Operator’s Manual instructions. During calibration and planting, the flowability of each formulation was noted, as well as any other calibration problems. There were no calibration or delivery problems with any treatment.

AGRONOMIC INFORMATION,

WEATHER DATA AND MATERIALS TESTED AND PICTURES

OF RESEARCH PROJECTS

Agronomic information and field insecticide history for each test plot location are listed in Appendix I.

Weather data from the test site or the nearest Iowa Climatological Station are listed in Appendix II. Information on materials tested is listed in Appendix III. Pictures of various activities of research projects are listed in Appendix IV.

RESEARCH SUPPORT

Many thanks to the Iowa Agriculture and Home Economics Experiment Station and the following companies for providing support for the evaluation of insecticides and plant-incorporated protectants (Corn Pests Research Project): AMVAC Chemical Corporation, Dow AgroSciences, Monsanto, Pioneer Hi-Bred International, Inc., Syngenta.

WAIVER OF ENDORSEMENT

This report deals with the relative ability of each treatment to protect corn from damage by soil insects. This information is not presented to endorse the use of any product and the name of Iowa State University should not appear in any advertising without prior written consent. Iowa State University, their respective officers, agents, or employees, have not made, and do not hereby make, any representation, warranty or covenant with respect to the use of these test results, nor will they be liable for any damages, losses, or claims, including those of an incidental or consequential nature, arising out of the use of these test results.

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Table 1. Iowa evaluation of insecticides and plant-incorporated protectants.

1All Studies were conducted on “trap crop corn” ground unless noted otherwise in parenthesis. (SB) = test conducted on ground that was planted to soybeans in 2010.

Experimental Unit Size Target Pest and Table Entries/ Row # Test Location Type of Studies1 Numbers Test Length (ft) Reps Corn Rootworms Ames

1)Pioneer CMRA study 2)Pioneer dual pyramid

efficacy and yield study

2-4 5-7

4 6

25 40

6 4

3)Monsanto corn rootwormstudy

8-10 8 40 4

4)Monsanto genuity VT3P seed blend study

11-12 6 100 1

5)Monsanto smartstax features & benefits study

13-14 7 200 1

6)Syngenta experimental hybrid efficacy study

15 12 20 4

7)Syngenta experimental hybrid efficacy study

16 12 20 4

Crawfordsville 1)Yield study 17-19 14 75 4 2)Monsanto smartstax RIB 20-21 6 100 1 3)Monsanto smartstax

features & benefits study 22-23 7 100 1

Northeast Iowa 1)Monsanto corn rootwormstudy

24-27 8 40 4

Nashua

1)Yield study 2)Monsanto corn rootworm

study

28-31 32-35

14 8

75 40

4 4

European Corn borer

Ames

1)Pioneer Optimum AcreMax II study (SB)

36-39 5 40 4

Corn Earworm

Ames

1)Dow AgroSciences smartstax vs HXX & RR2 traits study (SB)

40-42 3 20 4

2)Monsanto smartstax features & benefits study

43-44 7 200 1

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Table 2. Average root-injury and percent product consistency for the Pioneer CMRA study: Ames, IA.1

Node- Product

Treatment2 Injury3,4,5 Consistency6,7

HXX with Bio-insecticide8,9,10 0.06a 94a HXX 0.07a 97a HX1 with Bio-insecticide8,9,10 0.16 b 80ab HX1 0.27 c 61 b

1 Planted May 11, 2011; node injury evaluated August 15, 2011 2 HXX = Herculex XTRA hybrid (Pioneer P1395XR); HX1 = Herculex 1 hybrid (Pioneer P1395HR) 3 Chemical and check means based on 36 observations (6 roots/2 rows x 6 replications) 4 Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten

5 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 6 Product consistency = percentage of times nodal injury was 0.25 (¼ node eaten) or less 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Metarhizium anisopliae bioinsecticide (fungus) applied at 208 grams per plot; Steinernema carpocapsae and Heterorhabditis bacteriophora bioinsecticide (nematodes) solution applied at a rate of 100 nematodes per square inch.

9 Metarhizium anisopliae broadcast over plots by hand before planting then were incorporated by tiller shortly thereafter 10 Steinernema carpocapsae and Heterorhabditis bacteriophora were applied along each row approximately 6-9 inches from corn

plants and 2 inches deep

Table 3. Average stand count for the Pioneer CMRA study: Ames, IA.1

Stand

Treatment2 Count3,4

HX1 with Bio-insecticide5,6,7 35.75a HXX with Bio-insecticide5,6,7 35.00ab HXX 34.75ab HX1 34.00 b

1 Planted May 11, 2011; evaluated June 7 and September 21, 2011 2 HXX = Herculex XTRA hybrid (Pioneer P1395XR); HX1 = Herculex 1 hybrid (Pioneer P1395HR) 3 Means based on 24 observations (4 row treatment x 20 row-feet/treatment x 6 replications) 4 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 5 Metarhizium anisopliae bioinsecticide (fungus) applied at 208 grams per plot; Steinernema carpocapsae and Heterorhabditis bacteriophora bioinsecticide (nematodes) solution applied at a rate of 100 nematodes per square inch.



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Table 4. Average yield for the Pioneer CMRA study: Ames, IA.1

Treatment2 Yield3,4,5

HX1 with Bio-insecticide 6,7,8 202 HXX with Bio-insecticide 6,7,8 194 HXX 188 HX1 183

1 Planted May 11, 2011; Harvested (mechanically) October 13, 2011 2 HXX = Herculex XTRA hybrid (Pioneer P1395XR); HX1 = Herculex 1 hybrid (Pioneer P1395HR) 3 Means based on 6 observations (4-rows x 20 row-feet/treatment x 6 replications)

4 No significant differences between means (ANOVA, P < 0.05) 5 Yields converted to15% Moisture

6 Metarhizium anisopliae bioinsecticide (fungus) applied at 208 grams per plot; Steinernema carpocapsae and Heterorhabditis bacteriophora bioinsecticide (nematodes) solution applied at a rate of 100 nematodes per square inch.



Table 5. Average stand count for the Pioneer dual pyramid efficacy and yield study for corn rootworm: Ames, IA.1

Stand

Treatment2,3 Count4,5,6

HXX + MIR604 (Pure stand) 68.75 HXX + MIR604 (95% HXX + MIR604; 5% RR2 blended refuge) 67.75 HXX + MIR604 (90% HXX + MIR604; 10% RR2 blended refuge) 67.75 RR2 + Force 3G - 0.12/T-band 67.25 RR2 (Pure stand) with Poncho 1250/VOTIVO 67.25 RR2 (Pure stand) with Cruiser 250 67.25

1 Planted May 10, 2011; evaluated June 16, 2011 2 HXX + MIR604 = Dual Pyramided hybrid (Pioneer product TQ38148214); RR2 = Roundup Ready® Corn 2 trait (Pioneer® hybrid P0413R)

3 HXX = Herculex® XTRA technology. Herculex® Insect Protection technology by Dow AgroSciences and Pioneer Hi-Bred. . ®Herculex is a registered trademark of Dow AgroSciences LLC. RR2 = Roundup Ready® Corn 2 gene. ® Roundup Ready is a registered trademark of Monsanto Company Force® 3G is a registered trademark of a Syngenta Group Company

4 Means based on 16 observations (4 row treatment x 38 row-feet/treatment x 4 replications) 5 Insecticide listed as ounces a.i. per 1,000 row-feet; T-band = Insecticide applied at planting time

6 No significant differences between means (ANOVA, P < 0.05)

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Table 6. Average root-injury and percent product consistency for the Pioneer dual pyramid efficacy and yield study for corn rootworm: Ames, IA,1

Node- Product

Treatment2.3,4 Injury5,6 Consistency7,8

RR2 (Pure stand) + Force 3G - 0.12/T-band10 0.04a 98a HXX + MIR604 (90% HXX + MIR604)11 0.05a 96a HXX + MIR604 (Pure stand)9 0.11ab 88ab HXX + MIR604 (95% HXX + MIR604)12 0.18 b 73ab RR2 (Pure stand) with Poncho1250/VOTIVO10 0.19 bc 75ab RR2 blended refuge (10%)11 0.30 cd 50ab RR2 blended refuge (5%)11 0.33 cd 57 b RR2 (Pure stand) with Cruiser25010 0.39 d 54 b

1 Planted May 10, 2011; node injury evaluated August 15, 2011 2 HXX + MIR604 = Dual Pyramided hybrid (Pioneer product TQ38148214); RR2 = Roundup Ready® Corn 2 trait (Pioneer® hybrid P0413R)

3 HXX = Herculex® XTRA technology. Herculex® Insect Protection technology by Dow AgroSciences and Pioneer Hi-Bred. ®Herculex is a registered trademark of Dow AgroSciences LLC. RR2 = Roundup Ready® Corn 2 gene. ® Roundup Ready is a registered trademark of Monsanto Company Force® 3G is a registered trademark of a Syngenta Group Company

4 Insecticide listed as ounces a.i. per 1,000 row-feet; T-band = Insecticide applied at planting time

5 Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten

6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 7 Product consistency = percentage of times nodal injury was 0.25 (¼ node eaten) or less 8 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 9 For the pure stand treatment of HXX + MIR604, 48 Bt roots were dug (12 roots/2 rows x 4 replications)

10 For the pure stand treatments of RR2, 48 non-Bt roots were dug (12 roots/2 rows x 4 replications) 11 For the RR2 blended refuge treatments (5% & 10%), 24 non-Bt roots were dug (6 roots/2 rows x 4 replications) 12 For the pyramided treatments (90% & 95%), 48 Bt roots were dug (12 roots/2 rows x 4 replications)

Table 7. Average yield for the Pioneer dual pyramid efficacy and yield study for corn rootworm: Ames, IA.1

Treatment2,3,4 Yield5,6,7

RR2 (Pure stand) with Poncho 1250/VOTIVO 201a RR2 + Force 3G - 0.12/T-band 185ab HXX + MIR604 (Pure stand) 184ab HXX + MIR604 (95% HXX + MIR604; 5% RR2 blended refuge) 184ab RR2 (Pure stand) with Cruiser 250 177 bc HXX + MIR604 (90% HXX + MIR604; 10% RR2 blended refuge) 166 c

1 Planted May 10, 2011; machine harvested October 13, 2011 2 HXX + MIR604 = Dual Pyramided hybrid (Pioneer product TQ38148214); RR2 = Roundup Ready® Corn 2 trait (Pioneer hybrid P0413R)

3 HXX = Herculex® XTRA technology. Herculex® Insect Protection technology by Dow AgroSciences LLC and Pioneer Hi-Bred. ®Herculex is a registered trademark of Dow AgroSciences LLC. RR2 = Roundup Ready® Corn 2 gene. ® Roundup Ready is a registered trademark of Monsanto Company Force® 3G is a registered trademark of a Syngenta Group Company.

4 Insecticide listed as ounces a.i. per 1,000 row-feet; T-band = Insecticide applied at planting time 5 Means based on 4 observations (2-rows x 38 row-feet/treatment x 4 reps) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

7 Yields converted to15% Moisture

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Table 8. Average root-injury and product consistency for the Monsanto corn rootworm study: Ames, IA.1

Node- Product Treatment2 Form. Rate3 Placement4 Injury5,6,7 Consistency8,9

YGVT3 + Force 3.0G 0.12 T-band 0.04a 95a YGVT3 + Aztec 2.1G 0.14 T-band 0.06a 95a DeKalb-Iso + Aztec 2.1G 0.14 T-band 0.10a 85a DeKalb-Iso + Force 3.0G 0.12 T-band 0.10a 85a YGVT3 ------ ------ ------ 0.10ab 95a Smartstax ------ ------ ------ 0.12ab 80 b DeKalb-Iso ------ ------ ------ 0.22 bc 90a My-HXT ------ ------ ------ 0.32 c 55 b 1 Planted May 10, 2011; evaluated August 15, 2011

2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92); YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less 9 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

Table 9. Average stand count for the Monsanto corn rootworm study: Ames, IA.1

Stand

Treatment2 Form. Rate3 Placement4 Counts5,6

DeKalb-Iso ------ ------ ------ 36.25a My-HXT ------ ------ ------ 36.00ab DeKalb-Iso + Aztec 2.1G 0.14 T-band 35.50abc Smartstax ------ ------ ------ 35.50abc DeKalb-Iso + Force 3.0G 0.12 T-band 35.25abc YGVT3 ------ ------ ------ 35.25abc YGVT3 + Aztec 2.1G 0.14 T-band 34.00 bc YGVT3 + Force 3.0G 0.12 T-band 33.50 c

1 Planted May 10, 2011; evaluated June 7 and September 21, 2011 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92); YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = Insecticide applied at planting time

5 Means based on 32 observations (4 rows/ treatment x 17.5 row-ft/treatment x 4 replications x 2 evaluations) 6 No significant differences between means (ANOVA, P < 0.05)

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Table 10. Average yield for the Monsanto corn rootworm study: Ames, IA.1

Bushels/ Treatment2 Form. Rate3 Placement4 Acre5,6,7

My-HXT ------ ------ ------ 192

DeKalb-Iso ------ ------ ------ 174 DeKalb-Iso + Force 3.0G 0.12 T-band 168 YGVT3 + Aztec 2.1G 0.14 T-band 167 YGVT3 + Force 3.0G 0.12 T-band 165 YGVT3 ------ ------ ------ 163 DeKalb-Iso + Aztec 2.1G 0.14 T-band 159 Smartstax ------ ------ ------ 159 1 Planted May 10, 2011; machine harvested October 13, 2011 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92); YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Means based on 4 observations (4-row treatment x 35 row-feet/treatment x 4 replications) 6 No significant differences between means (ANOVA, P < 0.05) 7 Yields converted to 15% Moisture

Table 11. Average stand counts for the Monsanto genuity VT3P seed blend efficacy study: Ames, IA.1

Stand

Treatment2

F Counts3,4

Genuity VT3P RIB (100% Bt corn) 36.25 Smartstax (95% Bt corn/5% non-Bt corn) 36.00 Smartstax (100% Bt corn) 35.75 Dekalb-RR2 35.25 Genuity VT3P RIB (90% Bt corn/10% non-Bt corn) 35.00 Genuity VT3P RIB (80 Bt corn/20% non-Bt corn) 34.75

1 Planted May 10, 2011; evaluated June 7 and September 22, 2011 2 DeKalb-RR2 = DeKalb brand RR (DKC 61-36); Smartstax (100% Bt corn) = DeKalb brand Smartstax (DKC 61-21); Smartstax (95% Bt corn/5% non-Bt corn) = DeKalb brand Smartstax Refuge in a bag (DKC61-21JRM); Genuity VT3P RIB (90% Bt corn/10% non-Bt corn) = DeKalb VT3P Refuge in a bag (DKC61-97DKL); Genuity VT3P RIB (100% Bt corn) = DeKalb brand VT3P Refuge in a bag (DKC61-97WTM); Genuity VT3P RIB (80 Bt corn/20% non-Bt corn) = DeKalb brand VT3P Refuge in a bag (DKC61-97BPY)

3 Means based on 8 observations (4 rows/ treatment x 17.5 row-feet/treatment x 2 evaluations) 4 No significant differences between means (ANOVA, P < 0.05)

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Table 12. Average yield for the Monsanto genuity VT3P seed blend efficacy study: Ames, IA.1

Bushels/ Treatment2 Acre3,4,5

Genuity VT3P RIB (80 Bt corn/20% non-Bt corn) 187 Genuity VT3P RIB (90% Bt corn/10% non-Bt corn) 173 Genuity VT3P RIB (100% Bt corn) 173 DeKalb-RR2 170 Smartstax (100% Bt corn) 149 Smartstax (95% Bt corn/5% non-Bt corn) 148 1 Planted May 10, 2011; machine harvested October 12, 2011 2 DeKalb-RR2 = DeKalb brand RR (DKC 61-36); Smartstax (100% Bt corn) = DeKalb brand Smartstax (DKC 61-21); Smartstax (95% Bt corn/5% non-Bt corn) = DeKalb brand Smartstax Refuge in a bag (DKC61-21JRM); Genuity VT3P RIB (90% Bt corn/10% non-Bt corn) = DeKalb VT3P Refuge in a bag (DKC61-97DKL); Genuity VT3P RIB (100% Bt corn) = DeKalb brand VT3P Refuge in a bag (DKC61-97WTM); Genuity VT3P RIB (80 Bt corn/20% non-Bt corn) = DeKalb brand VT3P Refuge in a bag (DKC61-97BPY)

3 Means based on 1 observation (4-row treatment x 95 row-feet/treatment) 4 No significant differences between means (ANOVA, P < 0.05) 5 Yields converted to 15% Moisture

Table 13. Average stand count for the Monsanto genuity smartstax features and benefits study: Ames, IA.1

Stand


DeKalb-RR2 + Force 3.0G 0.12 T-band 35.25 Smartstax ------ ------ ------ 34.75 Agrisure1 + Force 3.0G 0.12 T-band 33.50 DeKalb-RR2 ------ ------ ------ 33.25 Pioneer-HXX ------ ------ ------ 33.00 Agrisure2 ------ ------ ------ 32.50 Pioneer-RR2 + Force 3.0G 0.12 T-band 32.50

1 Planted May 10, 2011; evaluated June 7 and September 28, 2011 2 Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 35F34PDR); Agrisure1 = Syngenta Agrisure GT hybrid (N53-W2); DeKalb-RR2 = DeKalb brand RR (DKC 55-08); Agrisure2 = Syngenta Agrisure 3111 hybrid (N53C-3111); Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 35F37) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = Insecticide applied at planting time


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Table 14. Average yield for the Monsanto genuity smartstax features and benefits study: Ames, IA.1


Pioneer-RR2 + Force 3.0G 0.12 T-band 162 DeKalb-RR2 + Force 3.0G 0.12 T-band 160 Agrisure1 + Force 3.0G 0.12 T-band 160 Agrisure2 ------ ------ ------ 155 DeKalb-RR2 ------ ------ ------ 150 Smartstax ------ ------ ------ 148 Pioneer-HXX ------ ------ ------ 143 1 Planted May 10, 2011; machine harvested October 12, 2011 2 Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 35F34PDR); Agrisure1 = Syngenta Agrisure GT hybrid (N53-W2); DeKalb-RR2 = DeKalb brand RR (DKC 55-08); Agrisure2 = Syngenta Agrisure 3111 hybrid (N53C-3111); Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 35F37) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Means based on 4 observations (4-row treatment x 195 row-feet/treatment) 6 No significant differences between means (ANOVA, P < 0.05) 7 Yields converted to 15% Moisture

Table 15. Average root-injury and percent product consistency for the Syngenta experimental hybrid efficacy study:

Old Dairy Farm, Ames, IA.1

Node ProductTreatment2,3 Rate4 Placement5 Injury6,7,8

Consistency9,10

MIR604 + HXRW + HX1 + Bt11 ------ ------ 0.01a 100a MIR604 + HXRW + HX1 + Bt11 + Force® 0.12 T-band 0.01a 100a 95% MIR604 + HX1 + Bt11 + HXRW / 5% refuge + Force® 0.12 T-band 0.01a 100a MIR604 + Bt11 + Force® 0.12 T-band 0.02a 100a 90% MIR604 + HX1 + Bt11 + HXRW / 10% refuge + Force® 0.12 T-band 0.02a 100a MIR162 + MIR604 + Bt11 + Force® 0.12 T-band 0.02a 100a 95% MIR604 + HX1 + Bt11 + HXRW / 5% refuge ------ ------ 0.03a 100a 90% MIR604 + HX1 + Bt11 + HXRW / 10% refuge ------ ------ 0.03a 100a MIR604 + Bt11 ------ ------ 0.05a 100a MIR162 + MIR604 + Bt11 ------ ------ 0.07a 92ab Non--Bt + Force® 0.12 T-band 0.08a 92ab Non-Bt ------ ------ 0.20 b 79 b

1 Planted May 18, 2011; evaluated August 18, 2011 2 MIR604 is the Agrisure® RW trait; MIR162 is the Agrisure Viptera™ trait for above-ground lepidopteran protection 3 Refuge corn does not contain rootworm active Bt but does have Bt11; HXRW = Herculex ® rootworm; Bt11 = Agrisure CB/LL trait for above-ground lepidopteran protection (e.g., corn borer); HX1 = Herculex I (lepidopteran protection)

4 Insecticide listed as ounces a.i. per 1,000 row-feet 5 T-band = Insecticide applied at planting time 6 Chemical and check means based on 24 observations (6 roots/2 rows x 4 replications) 7 Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten

8 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 9 Product consistency = percentage of times nodal injury was 0.25 (¼ node eaten) or less

10 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) Force® is a Restricted Use Pesticide.

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Table 16. Average root-injury and percent product consistency for the Syngenta experimental hybrid efficacy study: South Dairy Farm, Ames, IA.1

Node ProductTreatment2,3 Rate4 Placement5 Injury6,7,8 Consistency9,10

MIR604 + HXRW + HX1 + Bt11 ------ ------ 0.02a 100 95% MIR604 + HX1 + Bt11 + HXRW / 5% refuge ------ ------ 0.02a 100 MIR604 + HXRW + HX1 + Bt11 + Force® 0.12 T-band 0.03a 100 95% MIR604 + HX1 + Bt11 + HXRW / 5% refuge + Force® 0.12 T-band 0.03a 100 90% MIR604 + HX1 + Bt11 + HXRW / 10% refuge + Force® 0.12 T-band 0.03a 100 MIR162 + MIR604 + Bt11 + Force® 0.12 T-band 0.04a 92 90% MIR604 + HX1 + Bt11 + HXRW / 10% refuge ------ ------ 0.04a 100 MIR604 + Bt11 + Force® 0.12 T-band 0.06a 96 MIR162 + MIR604 + Bt11 ------ ------ 0.06a 100 MIR604 + Bt11 ------ ------ 0.07a 96 Non--Bt + Force® 0.12 T-band 0.08a 92 Non-Bt ------ ------ 0.18 b 83

1 Planted May 19, 2011; evaluated August 19, 2011 2 MIR604 is the Agrisure® RW trait; MIR162 is the Agrisure Viptera™ trait for above-ground lepidopteran protection 3 Refuge corn does not contain rootworm active Bt but does have Bt11; HXRW = Herculex ® rootworm; Bt11 = Agrisure CB/LL trait for above-ground lepidopteran protection (e.g., corn borer); HX1 = Herculex I (lepidopteran protection)

4 Insecticide listed as ounces a.i. per 1,000 row-feet 5 T-band = Insecticide applied at planting time 6 Chemical and check means based on 24 observations (6 roots/2 rows x 4 replications) 7 Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten

8 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 9 Product consistency = percentage of times nodal injury was 0.25 (¼ node eaten) or less

10 No significant differences between means (ANOVA, P < 0.05) Force® is a Restricted Use Pesticide. Table 17. Yield study: Crawfordsville, IA.1

Node- Product Treatment2,3 Form. Rate4 Placement5 Injury6,7,8 Consistency9,10

My-HXX + SmartChoice-SB 5G 0.18 In-Furrow 0.09a 90a DeKalb-Smartstax + Aztec 2.1G 0.14 In-Furrow 0.18ab 75ab My-HXX + Force 3.0G 0.12 In-Furrow 0.24 bc 60ab My-Smartstax ------ ----- ----- 0.28 bc 65ab My-HXX + Counter-SB 20G 0.90 In-Furrow 0.44 cd 45abc My-HXX ------ ----- ----- 0.51 d 25abc My-Iso + Force 3.0G 0.12 In-Furrow 0.56 de 25 bc DeKalb-Iso + Aztec 2.1G 0.14 In-Furrow 0.77 de 15 bc My-95%SSTX/5%Non-Bt11 ------ ----- ----- 0.99 e 8 bc DeKalb-Iso ------ ----- ----- 1.54 f 0 c My-Iso ------ ----- ----- 2.27 g 0 c

1 Planted May 5, 2011; evaluated August 11, 2011 2 My-Smartstax = Mycogen Smartstax (Mycogen 2K594); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Smartstax = DeKalb Smartstax (DKC61-21); DeKalb-Iso = DeKalb brand RR Isoline (DKC 61-72); My-Iso = Mycogen brand RR2 (Mycogen 2K591); My-95%SSTX/5%Non-Bt = Mycogen 95% Smartstax + 5% Non-Bt (Refuge in a Bag) (Mycogen 2K594+ Mycogen 2K591)

3 My-Iso (Mycogen 2K591) is the isoline of My-HXX (Mycogen 2K592) 4 Insecticide listed as ounces a.i. per 1,000 row-feet 5 In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 6 Chemical and check means (except My-95%SSTX/5%Non-Bt treatment) based on 20 observations (5 roots/2 rows x 4

replications) 7 Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten 8 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 9 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less

10 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 11 For the refuge in the bag treatment (My-95%SSTX/5% Non-Bt), mean based on 36 observations (9 roots/2 rows (6 Smartstax (3

adjacent roots and 3 distant roots to a Non-Bt plant) + 3 Non-Bt) x 4 replications)

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Table 18. Yield study: Crawfordsville, IA.1

Stand

Treatment2,3 Form. Rate4 Placement5 Count6,7

My-95%SSTX/5%Non-Bt ------ ----- ----- 36.00a My-HXX + SmartChoice-SB 5G 0.18 In-Furrow 35.25ab My-Smartstax ------ ----- ----- 35.25ab DeKalb-Iso ------ ----- ----- 34.50ab DeKalb-Smartstax + Aztec 2.1G 0.14 In-Furrow 34.50ab My-HXX + Force 3.0G 0.12 In-Furrow 34.50ab My-Iso + Force 3.0G 0.12 In-Furrow 34.25ab My-HXX ------ ----- ----- 34.00ab DeKalb-Iso + Aztec 2.1G 0.14 In-Furrow 33.75ab My-HXX + Counter-SB 20G 0.90 In-Furrow 33.50 b My-Iso ------ ----- ----- 33.50 b

1 Planted May 5, 2011; evaluated June 8 and September 30, 2011 2 My-Smartstax = Mycogen Smartstax (Mycogen 2K594); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Smartstax = DeKalb Smartstax (DKC61-21); DeKalb-Iso = DeKalb brand RR Isoline (DKC 61-72); My-Iso = Mycogen brand RR2 (Mycogen 2K591); My-95%SSTX/5%Non-Bt = Mycogen 95% Smartstax + 5% Non-Bt (Refuge in a Bag) (Mycogen 2K594+ Mycogen 2K591)

3 My-Iso (Mycogen 2K591) is the isoline of My-HXX (Mycogen 2K592) 4 Insecticide listed as ounces a.i. per 1,000 row-feet 5 In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 6 Means based on 16 observations (2-row treatment x 17.5 row-feet/treatment x 4 replications x 2 evaluation dates) 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) Table 19. Yield study: Crawfordsville, IA.1

Bushels/

Treatment2,3 Form. Rate4 Placement5 Acre6,7,8

My-HXX + SmartChoice-SB 5G 0.18 In-Furrow 155a My-HXX + Force 3.0G 0.12 In-Furrow 152a My-HXX + Counter-SB 20G 0.90 In-Furrow 142ab My-95%SSTX/5%Non-Bt ------ ----- ----- 142ab My-Smartstax ------ ----- ----- 141ab DeKalb-Iso + Aztec 2.1G 0.14 In-Furrow 139ab My-HXX ------ ----- ----- 139ab DeKalb-Iso ------ ----- ----- 128ab DeKalb-Smartstax + Aztec 2.1G 0.14 In-Furrow 119ab My-Iso + Force 3.0G 0.12 In-Furrow 118ab My-Iso ------ ----- ----- 111 b

1 Planted May 5, 2011; machine harvested October 7, 2011 2 My-Smartstax = Mycogen Smartstax (Mycogen 2K594); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Smartstax = DeKalb Smartstax (DKC61-21); DeKalb-Iso = DeKalb brand RR Isoline (DKC 61-72); My-Iso = Mycogen brand RR2 (Mycogen 2K591); My-95%SSTX/5%Non-Bt = Mycogen 95% Smartstax + 5% Non-Bt (Refuge in a Bag) (Mycogen 2K594+ Mycogen 2K591)

3 My-Iso (Mycogen 2K591) is the isoline of My-HXX (Mycogen 2K592) 4 Insecticide listed as ounces a.i. per 1,000 row-feet 5 In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 6 Means based on 4 observations (2-row treatment x 69 row-feet/treatment x 4 replications) 7 No significant differences between means (ANOVA, P < 0.05) 8 Yields converted to15% Moisture

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Table 20. Average stand count for the Monsanto smartstax refuge in a bag study: Crawfordsville, IA.1

Stand Treatment2 Form. Rate3 Placement4 Counts5,6

DeKalb-RR2 + Aztec 2.1G 0.14 T-band 34.25 Smartstax RIB (95%Bt/5%non-Bt) ------ ------ ------ 34.00 DeKalb-RR2 ------ ------ ------ 33.75 Smartstax ------ ------ ------ 33.75 Pioneer-HXX ------ ------ ------ 33.50 Pioneer OAM1 ------ ------ ------ 33.00

1 Planted May 5, 2011; evaluated June 8 and September 30, 2011 2 Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer OAM1 = Pioneer Optimum AcreMax (Pioneer P0528XR); Smartstax (95% Bt corn/5% non-Bt corn) = DeKalb brand Smartstax Refuge in a bag (DKC55-08JRM); Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer P0528AMI); DeKalb-RR2 = DeKalb brand RR (DKC 55-08)

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Means based on 8 observations (4 rows/ treatment x 17.5 row-feet/treatment x 2 evaluations) 6 No significant differences between means (ANOVA, P < 0.05)

Table 21. Average yield for the Monsanto smartstax refuge in a bag study: Crawfordsville, IA.1


Smartstax RIB (95%Bt/5%non-Bt) ------ ------ ------ 194 Smartstax ------ ------ ------ 165 Pioneer-HXX ------ ------ ------ 155 Pioneer OAM1 ------ ------ ------ 148 DeKalb-RR2 + Aztec 2.1G 0.14 T-band 143 DeKalb-RR2 ------ ------ ------ 142 1 Planted May 5, 2011; machine harvested October 7, 2011 2 Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer OAM1 = Pioneer Optimum AcreMax (Pioneer P0528XR); Smartstax (95% Bt corn/5% non-Bt corn) = DeKalb brand Smartstax Refuge in a bag (DKC55-08JRM); Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer P0528AMI); DeKalb-RR2 = DeKalb brand RR (DKC 55-08)

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Means based on 1 observation (4-row treatment x 95 row-feet/treatment) 6 No significant differences between means (ANOVA, P < 0.05) 7 Yields converted to 15% Moisture

23

Table 22. Average stand count for the Monsanto genuity smartstax features and benefits study: Crawfordsville, IA.1

Stand


Smartstax ------ ------ ------ 34.25a DeKalb-RR2 + Force 3.0G 0.12 T-band 33.25ab DeKalb-RR2 ------ ------ ------ 33.00ab Pioneer-HXX ------ ------ ------ 32.25ab Pioneer-RR2 + Force 3.0G 0.12 T-band 32.00ab Agrisure1 + Force 3.0G 0.12 T-band 31.75ab Agrisure2 ------ ------ ------ 30.00 c

1 Planted May 5, 2011; evaluated June 7 and September 28, 2011 2 Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 35F34PDR); Agrisure1 = Syngenta Agrisure GT hybrid (N53-W2); DeKalb-RR2 = DeKalb brand RR (DKC 55-08); Agrisure2 = Syngenta Agrisure 3111 hybrid (N53C-3111); Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 35F37) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = Insecticide applied at planting time


Table 23. Average yield for the Monsanto genuity smartstax features and benefits study: Crawfordsville, IA.1


Pioneer-HXX ------- ------ ------ 160 DeKalb-RR2 ------- ------ ------ 159 Smartstax ------- ------ ------ 159 DeKalb-RR2 + Force 3.0G 0.12 T-band 153 Pioneer-RR2 + Force 3.0G 0.12 T-band 141 Agrisure2 ------ ------ ------ 133 Agrisure1 + Force 3.0G 0.12 T-band 114 1 Planted May 5, 2011; machine harvested October 12, 2011 2 Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 35F34PDR); Agrisure1 = Syngenta Agrisure GT hybrid (N53-W2); DeKalb-RR2 = DeKalb brand RR (DKC 55-08); Agrisure2 = Syngenta Agrisure 3111 hybrid (N53C-3111); Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 35F37) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Means based on 4 observations (4-row treatment x 95 row-feet/treatment) 6 No significant differences between means (ANOVA, P < 0.05) 7 Yields converted to 15% Moisture

24

Table 24. Average root-injury and product consistency for the Monsanto corn rootworm study: Northeast Iowa.1


YGVT3 + Aztec 2.1G 0.14 T-band 0.30a 65a DeKalb-Iso + Aztec 2.1G 0.14 T-band 0.33a 45a Smartstax ------ ------ ------ 0.37a 55a YGVT3 + Force 3.0G 0.12 T-band 0.57a 35ab DeKalb-Iso + Force 3.0G 0.12 T-band 1.23 b 35ab My-HXT ------ ------ ------ 1.25 b 0 c YGVT3 ------ ------ ------ 1.90 c 5 bc DeKalb-Iso ------ ------ ------ 2.50 d 0 c 1 Planted May 3, 2011; evaluated August 10, 2011


Table 25. Average stand count for the Monsanto corn rootworm study: Northeast Iowa.1

Stand


DeKalb-Iso + Aztec 2.1G 0.14 T-band 36.50 YGVT3 + Force 3.0G 0.12 T-band 36.25 DeKalb-Iso ------ ------ ------ 36.00 My-HXT ------ ------ ------ 35.75 YGVT3 ------ ------ ------ 35.25 YGVT3 + Aztec 2.1G 0.14 T-band 35.25 DeKalb-Iso + Force 3.0G 0.12 T-band 35.00 Smartstax ------ ------ ------ 33.50


5 Means based on 16 observations (4 row treatment x 17.5 row-feet/treatment x 4 replications) 6 No significant differences between means (ANOVA, P < 0.05)

25

Table 26. Average percent lodging for the Monsanto corn rootworm study: Northeast Iowa.1

%

Treatment2 Form. Rate3 Placement4 Lodging5,6

YGVT3 + Force 3.0G 0.12 T-band 1a DeKalb-Iso + Aztec 2.1G 0.14 T-band 3a Smartstax ------ ------ ------ 6a DeKalb-Iso + Force 3.0G 0.12 T-band 7a YGVT3 + Aztec 2.1G 0.14 T-band 11a My-HXT ------ ------ ------ 17a YGVT3 ------ ------ ------ 57 b DeKalb-Iso ------ ------ ------ 100 c

1 Planted May 3, 2011; evaluated September 16, 2011 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92); YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Means based on 16 observations (4-row treatment x17.5 row-feet/treatment x 4 replications) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

Table 27. Average yield for the Monsanto corn rootworm study: Northeast Iowa.1


DeKalb-Iso + Aztec 2.1G 0.14 T-band 231 YGVT3 + Force 3.0G 0.12 T-band 225 YGVT3 + Aztec 2.1G 0.14 T-band 224 My-HXT ------ ------ ------ 2218

Smartstax ------ ------ ------ 220 DeKalb-Iso + Force 3.0G 0.12 T-band 203 DeKalb-Iso ------ ------ ------ 195 YGVT3 ------ ------ ------ 188 1 Planted May 3, 2011; machine harvested October 4, 2011 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92); YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Means based on 4 observations (4-row treatment x 35 row-feet/treatment x 4 replications) 6 No significant differences between means (ANOVA, P < 0.05) 7 Yields converted to15% Moisture 8 Means based on 3 observations (4-row treatment x 35 row-feet/treatment x 3 replications)

26

Table 28. Yield study: Nashua, IA.1

Node- ProductTreatment2,3 Form. Rate4 Placement5 Injury6,7,8 Consistency9,10

DeKalb-Smartstax + Aztec 2.1G 0.14 In-Furrow 0.00a 100a My-HXX + SmartChoice-SB 5G 0.18 In-Furrow 0.24 b 75ab My-Smartstax ----- ----- ----- 0.38 bc 55ab DeKalb-Iso + Aztec 2.1G 0.14 In-Furrow 0.64 cd 15 bc My-HXX + Force 3.0G 0.12 In-Furrow 0.68 cd 35abc My-HXX + Counter-SB 20G 0.90 In-Furrow 0.85 d 15 bc My-95%SSTX/5%Non-Bt11 ----- ----- ----- 0.88 cd 50ab My-HXX ----- ----- ----- 1.03 de 15 bc My-Iso + Force 3.0G 0.12 In-Furrow 1.51 ef 0 c DeKalb-Iso ----- ----- ----- 2.27 fg 0 c My-Iso ----- ----- ----- 2.51 g 0 c

1 Planted May 4, 2011; evaluated August 8, 2011

2 My-Smartstax = Mycogen Smartstax (Mycogen 2K594); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Smartstax = DeKalb Smartstax (DKC61-21); DeKalb-Iso = DeKalb brand RR Isoline (DKC 61-72); My-Iso = Mycogen brand RR2 (Mycogen 2K591); My-95%SSTX/5%Non-Bt = Mycogen 95% Smartstax + 5% Non-Bt (Refuge in a Bag) (Mycogen 2K594+ Mycogen 2K591)

3 My-Iso (Mycogen 2K591) is the isoline of My-HXX (Mycogen 2K592) 4 Insecticide listed as ounces a.i. per 1,000 row-feet 5 In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 6 Chemical and check means (except My-95%SSTX/5%Non-Bt treatment) based on 20 observations (5 roots/2 rows x 4

replications) 7 Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten 8 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 9 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less

10 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 11 For the refuge in the bag treatment (My-95%SSTX/5%Non-Bt), mean based on 36 observations (9 roots/2 rows (6 Smartstax (3

adjacent roots and 3 distant roots to a Non-Bt plant) + 3 Non-Bt) x 4 replications) Table 29. Yield study: Nashua, IA. 1

Stand Treatment2,3 Form. Rate4 Placement5 Count6,7

My-Iso + Force 3.0G 0.12 In-Furrow 36.00 DeKalb-Iso + Aztec 2.1G 0.14 In-Furrow 35.75 My-HXX + SmartChoice-SB 5G 0.18 In-Furrow 35.50 My-Smartstax ----- ----- ----- 35.50 My-HXX + Counter-SB 20G 0.90 In-Furrow 35.25 DeKalb-Smartstax + Aztec 2.1G 0.14 In-Furrow 35.25 My-HXX ----- ----- ----- 34.50 My-Iso ----- ----- ----- 34.50 My-HXX + Force 3.0G 0.12 In-Furrow 34.25 DeKalb-Iso ----- ----- ----- 34.25 My-95%SSTX/5%Non-Bt ----- ----- ----- 34.00

1 Planted May 4, 2011; evaluated June 7 & September 23, 2011 2 My-Smartstax = Mycogen Smartstax (Mycogen 2K594); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Smartstax = DeKalb Smartstax (DKC61-21); DeKalb-Iso = DeKalb brand RR Isoline (DKC 61-72); My-Iso = Mycogen brand RR2 (Mycogen 2K591); My-95%SSTX/5%Non-Bt = Mycogen 95% Smartstax + 5% Non-Bt (Refuge in a Bag) (Mycogen 2K594+ Mycogen 2K591)

3 My-Iso (Mycogen 2K591) is the isoline of My-HXX (Mycogen 2K592) 4 Insecticide listed as ounces a.i. per 1,000 row-feet 5 In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 6 Means based on 16 observations (2-row treatment x17.5 row-feet/treatment x 4 replications x 2 evaluation dates) 7 No significant differences between means (ANOVA, P < 0.05)

27

Table 30. Yield study: Nashua, IA.1

% Treatment2,3 Form. Rate4 Placement5 Lodging6,7

My-Iso + Force 3.0G 0.12 In-Furrow 0a My-Smartstax ----- ----- ----- 0a My-95%SSTX/5%Non-Bt ----- ----- ----- 0a My-HXX + Force 3.0G 0.12 In-Furrow 0a My-HXX ----- ----- ----- 0a My-HXX + Counter-SB 20G 0.90 In-Furrow 0a My-HXX + SmartChoice-SB 5G 0.18 In-Furrow 0a DeKalb-Iso + Aztec 2.1G 0.14 In-Furrow 0a DeKalb-Smartstax + Aztec 2.1G 0.14 In-Furrow 1a My-Iso ------ ------ ------ 37 b DeKalb-Iso ------ ------ ------ 61 b

1 Planted May 4, 2011; evaluated September 23, 2011 2 My-Smartstax = Mycogen Smartstax (Mycogen 2K594); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Smartstax = DeKalb Smartstax (DKC61-21); DeKalb-Iso = DeKalb brand RR Isoline (DKC 61-72); My-Iso = Mycogen brand RR2 (Mycogen 2K591); My-95%SSTX/5%Non-Bt = Mycogen 95% Smartstax + 5% Non-Bt (Refuge in a Bag) (Mycogen 2K594+ Mycogen 2K591)

3 My-Iso (Mycogen 2K591) is the isoline of My-HXX (Mycogen 2K592) 4 Insecticide listed as ounces a.i. per 1,000 row-feet 5 In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 6 Means based on 8 observations (2-row treatment x17.5 row-feet/treatment x 4 replications) 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) Table 31. Yield study: Nashua, IA.1

Bushels/ Treatment2,3 Form. Rate4 Placement5 Acre6,7,8

DeKalb-Iso + Aztec 2.1G 0.14 In-Furrow 212a My-HXX + SmartChoice-SB 5G 0.18 In-Furrow 207a My-Smartstax ----- ------ ------ 205a My-HXX + Force 3.0G 0.12 In-Furrow 200a My-HXX + Counter-SB 20G 0.90 In-Furrow 200a My-HXX ------ ------ ------ 199a DeKalb-Smartstax + Aztec 2.1G 0.14 In-Furrow 197a My-Iso + Force 3.0G 0.12 In-Furrow 197a My-95%SSTX/5%Non-Bt ------ ------ ------ 192a My-Iso ------ ------ ------ 169 b DeKalb-Iso ------ ------ ------ 166 b

1 Planted May 4, 2011; machine harvested October 14, 2011 2 My-Smartstax = Mycogen Smartstax (Mycogen 2K594); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Smartstax = DeKalb Smartstax (DKC61-21); DeKalb-Iso = DeKalb brand RR Isoline (DKC 61-72); My-Iso = Mycogen brand RR2 (Mycogen 2K591); My-95%SSTX/5%Non-Bt = Mycogen 95% Smartstax + 5% Non-Bt (Refuge in a Bag) (Mycogen 2K594+ Mycogen 2K591)

3 My-Iso (Mycogen 2K591) is the isoline of My-HXX (Mycogen 2K592) 4 Insecticide listed as ounces a.i. per 1,000 row-ft 5 In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 6 Means based on 4 observations (2-row treatment x 69 row-feet/treatment x 4 replications) 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Yields converted to15% Moisture

28

Table 32. Average root-injury and product consistency for the Monsanto corn rootworm study: Nashua, IA.1


Smartstax ------ ------ ------ 0.11a 90a YGVT3 + Aztec 2.1G 0.14 T-band 0.32 b 45 b YGVT3 + Force 3.0G 0.12 T-band 0.43 bc 40 b My-HXT ------ ------ ------ 0.53 bc 15 bc DeKalb-Iso + Aztec 2.1G 0.14 T-band 0.62 c 20 bc DeKalb-Iso + Force 3.0G 0.12 T-band 0.64 c 5 c YGVT3 ------ ------ ------ 1.41 d 5 c DeKalb-Iso ------ ------ ------ 2.13 e 0 c 1 Planted May 3, 2011; evaluated August 10, 2011


Table 33. Average stand count for the Monsanto corn rootworm study: Nashua, IA.1

Stand


DeKalb-Iso + Force 3.0G 0.12 T-band 35.75a My-HXT ------ ------ ------ 35.50a YGVT3 ------ ------ ------ 35.50ab DeKalb-Iso ------ ------ ------ 35.25ab YGVT3 + Aztec 2.1G 0.14 T-band 35.00ab DeKalb-Iso + Aztec 2.1G 0.14 T-band 34.75ab YGVT3 + Force 3.0G 0.12 T-band 34.25ab Smartstax ------ ------ ------ 33.50 b


5 Means based on 32 observations (4 row treatment x 17.5 row-feet/treatment x 4 replications x 2 evaluations) 6 No significant differences between means (ANOVA, P < 0.05)

29

Table 34. Average percent lodging for the Monsanto corn rootworm study: Nashua, IA.1

%

Treatment2 Form. Rate3 Placement4 Lodging5,6

DeKalb-Iso + Aztec 2.1G 0.14 T-band 0a DeKalb-Iso + Force 3.0G 0.12 T-band 0a Smartstax ------ ------ ------ 0a My-HXT ------ ------ ------ 0a YGVT3 + Aztec 2.1G 0.14 T-band 0a YGVT3 + Force 3.0G 0.12 T-band 0a YGVT3 ------ ------ ------ 3a DeKalb-Iso ------ ------ ------ 29 b

1 Planted May 3, 2011; evaluated September 16, 2011 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92); YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Means based on16 observations (4-row treatment x17.5 row-feet/treatment x 4 replications) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

Table 35. Average yield for the Monsanto corn rootworm study: Nashua, IA.1


My-HXT ------ ------ ------ 231a YGVT3 + Force 3.0G 0.12 T-band 210 b Smartstax ------ ------ ------ 208 b YGVT3 ------ ------ ------ 203 bc YGVT3 + Aztec 2.1G 0.14 T-band 201 bc DeKalb-Iso + Force 3.0G 0.12 T-band 201 bc DeKalb-Iso ------ ------ ------ 185 c DeKalb-Iso + Aztec 2.1G 0.14 T-band 182 c 1 Planted May 3, 2011; machine harvested October 4, 2011 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92); VGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61)

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Means based on 4 observations (4-row treatment x 35 row-feet/treatment x 4 replications) 6 No significant differences between means (ANOVA, P < 0.05) 7 Yields converted to 15% Moisture

30

Table 36. Average stand count for the Pioneer ECB Optimum AcreMax study: Ames, IA.1

Stand

Treatment2,3

F Count4,5

HX1 x YGCB (with 5% blended refuge plants) 68.25 HX1 x YGCB 68.00 HX1 x YGCB (with 10% blended refuge plants) 68.00 HX1 67.75 RR2 67.00

1 Planted May 10, 2011; evaluated June 7, 2011 2 HX1 = Herculex® 1 trait (Pioneer® hybrid P0461HR); RR2 = Roundup Ready® Corn 2 trait (Pioneer® hybrid P0461R); HX1 x YGCB = Pyramid Hybrid (Pioneer P0461YHR/P0461R); 3 HX1 = Herculex® 1 trait. Herculex® 1 Insect Protection technology by Dow AgroSciences LLC and Pioneer Hi-Bred. Liberty Link® (LL), Ignite® and the Water Droplet logo are registered trademarks of Bayer. YGCB = YieldGard® Corn Borer gene. ®YieldGard and the YieldGard Corn Borer design are registered trademarks of Monsanto Company. RR2 = Roundup Ready® Corn 2 gene. ® Roundup Ready is a registered trademark of Monsanto Company.

4 Means based on 16 observations (4 row treatment x 38 row-feet/treatment x 4 replications) 5 No significant differences between means (ANOVA, P < 0.05)

Table 37. Average whorl injury for the Pioneer ECB Optimum AcreMax study: Ames, IA.1

ECB whorl

Treatment2,3

F Injury4,5

HX1 (Pure stand)6 9.0a HX1 x YGCB (Pure stand)6 9.0a HX1 x YGCB (pyramid with 5% blended refuge plants)9 9.0a RR2 (5% blended refuge)8 9.0a HX1 x YGCB (pyramid with 10% blended refuge plants)9 8.9a RR2 (Pure stand)7 8.9a RR2 (10% blended refuge)8 8.8 b

1 Planted May 10, 2011; evaluated July 15, 2011 2 HX1 = Herculex® 1 trait (Pioneer® hybrid P0461HR); RR2 = Roundup Ready® Corn 2 trait (Pioneer® hybrid P0461R); HX1 x YGCB = Pyramid Hybrid (Pioneer P0461YHR/P0461R); 3 HX1 = Herculex®1 trait. Herculex® 1 Insect Protection technology by Dow AgroSciences LLC and Pioneer Hi-Bred. Liberty Link® (LL), Ignite® and the Water Droplet logo are registered trademarks of Bayer. YGCB = YieldGard® Corn Borer gene. ®YieldGard and the YieldGard Corn Borer design are registered trademarks of Monsanto Company. RR2 = Roundup Ready® Corn 2 gene. ® Roundup Ready is a registered trademark of Monsanto Company.

4 Means based on the Pioneer 9-1 rating scale for evaluating first generation ECB whorl injury (ECB1LF). 5 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 6 For the pure stand treatments of HX1 x YGCB, 48 Bt plants were evaluated (12 plants/2 rows x 4 replications)

7 For the pure stand treatment of RR2, 48 non-Bt roots were evaluated (12 plants/2 rows x 4 replications) 8 For the RR2 blended refuge treatments (5% & 10%), 24 non-Bt plants were evaluated (6 plants/2 rows x 4 replications) 9 For the pyramided treatments (90% & 95%), 48 Bt plants were evaluated (12 plants/2 rows x 4 replications)

31

Table 38. Average tunneling (cm) for the Pioneer ECB Optimum AcreMax study: Ames, IA.1

Average ECB Treatment2,3 Tunneling (cm)4

HX1 x YGCB (Pure stand)5 0.00a HX1 (Pure stand)5 0.07a HX1 x YGCB (pyramid with 5% blended refuge plants)8 0.34a HX1 x YGCB (pyramid with 10% blended refuge plants)8 0.43a RR2 (5% blended refuge)7 3.25 b RR2 (10% blended refuge)7 5.92 bc RR2 (Pure stand)6 6.88 c

1 Planted May 10, 2011; evaluated October 6 & 7, 2011 2 HX1 = Herculex® 1 trait (Pioneer® hybrid P0461HR); RR2 = Roundup Ready® Corn 2 trait (Pioneer® hybrid P0461R); HX1 x YGCB = Pyramid Hybrid (Pioneer P0461YHR/P0461R); 3 HX1 = Herculex® 1 trait. Herculex® 1 Insect Protection technology by Dow AgroSciences LLC and Pioneer Hi-Bred. Liberty Link® (LL), Ignite® and the Water Droplet logo are registered trademarks of Bayer. YGCB = YieldGard® Corn Borer gene. ®YieldGard and the YieldGard Corn Borer design are registered trademarks of Monsanto Company. RR2 = Roundup Ready® Corn 2 gene. ® Roundup Ready is a registered trademark of Monsanto Company.

4 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 5 For the pure stand treatments of HX1 x YGCB, 48 Bt plants were evaluated (12 plants/2 rows x 4 replications)

6 For the pure stand treatment of RR2, 48 non-Bt roots were evaluated (12 plants/2 rows x 4 replications) 7 For the RR2 blended refuge treatments (5% & 10%), 24 non-Bt plants were evaluated (6 plants/2 rows x 4 replications) 8 For the pyramided treatments (90% & 95%), 48 Bt plants were evaluated (12 plants/2 rows x 4 replications)

Table 39. Average yield for the Pioneer ECB Optimum AcreMax study: Ames, IA.1

Treatment2,3 Yield4,5,6

HX1 x YGCB 215 HX1 209 HX1 x YGCB with 5% blended refuge (RR2) 209 RR2 208 HX1 x YGCB with 10% blended refuge (RR2) 202

1 Planted May 10, 2011; machine harvested October 13, 2010 2 HX1 = Herculex® 1 trait (Pioneer® hybrid P0461HR); RR2 = Roundup Ready® Corn 2 trait (Pioneer® hybrid P0461R); HX1 x YGCB = Pyramid Hybrid (Pioneer P0461YHR/P0461R); 3 HX1 = Herculex® 1 trait. Herculex® 1 Insect Protection technology by Dow AgroSciences LLC and Pioneer Hi-Bred. Ignite® and the Water Droplet logo are registered trademarks of Bayer. YGCB = YieldGard® Corn Borer gene. ®YieldGard and the YieldGard Corn Borer design are registered trademarks of Monsanto Company. RR2 = Roundup Ready® Corn 2 gene. ® Roundup Ready is a registered trademark of Monsanto Company.

4 Means based on 4 observations (2-rows x 38 row-feet/treatment x 4 replications) 5 No significant differences between means (ANOVA, P < 0.05) 6 Yields converted to15% Moisture

32

Table 40. Stand counts for the Dow AgroSciences Smartstax vs Herculex XTRA and RR2 corn study, Ames, IA.1

Stand

Treatment2 Counts3,4

RR2 31.50a Smartstax 30.50ab HXX 28.50 b

1 Planted May 10, 2011; stand counts evaluated on June 7, 2011 2 RR2 = Mycogen 2K591; HXX = Mycogen Herculex XTRA 2K592; Smartstax = Mycogen 2K594 3 Stand counts based on 8 observations (2 rows/17.5 feet of row x 4 replications) 4 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

Table 41. Total kernels, damaged kernels, and percent damaged for the Dow AgroSciences Smartstax vs Herculex

XTRA and RR2 corn study, Ames, IA.1,2

Total Damaged % Treatment3 Kernels/Ear4 Kernels/Ear4,5 Damaged/Ear6,7

Smartstax 491 1.23a 0.25a HXX 519 3.80 b 0.73 b RR2 488 4.70 b 0.96 b

1 Planted May 10, 2011; evaluated August 18, 2011 2 Ten corn ears per row in rows 2 and 3 of each plot were infested on August 4, 2011 with 5 1st instar CEW larvae 3 RR2 = Mycogen 2K591; HXX = Mycogen Herculex XTRA 2K592; Smartstax = Mycogen 2K594 4 Mean total kernels, damaged kernels counts and % damaged based on 40 observations (10 ears/treatment x 4 replications) 5 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 6 Mean total kernels, damaged kernels counts and % damaged based on 40 observations (10 ears/treatment x 4 replications) 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) Table 42. Average kernel area (cm2) consumed and number of total damaged ears for the Dow AgroSciences

Smartstax vs Herculex XTRA and RR2 corn study, Ames, IA.1,2

Kernel Area Total Treatment3 Consumed (cm2)4,5 Damaged Ears6,7

Smartstax 0.42a 13a HXX 1.18 b 24 b RR2 1.46 b 26 b

1 Planted May 10, 2011; evaluated August 18, 2011 2 Ten corn ears per row in rows 2 and 3 of each plot were infested on August 4, 2011 with 5 1st instar CEW larvae 3 RR2 = Mycogen 2K591; HXX = Mycogen Herculex XTRA 2K592; Smartstax = Mycogen 2K594 4 Kernel area consumed based on 40 observations (10 ears/treatment x 4 replications) 5 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 6 Total damaged ears based on 40 observations (10 ears/treatment x 4 replications) 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

33

Table 43. Total kernels, damaged kernels, and percent damaged for the Monsanto genuity smartstax features and benefits study, Ames, IA.1,2

Total Damaged Percent Treatment3 Form. Rate4 Placement5 Kernels/Ear6 Kernels/Ear6,7 Damaged/Ear8,9

Agrisure2 ------ ------ ------ 442 0.0a 0.0a Smartstax ------ ------ ------ 411 0.0a 0.0a Pioneer-HXX ------ ------ ------ 461 4.7 b 1.0 b DeKalb-RR2 ------ ------ ------ 447 9.2 b 2.1 b DeKalb-RR2 + Force 2.1G 0.12 T-band 318 11.8 b 3.7 b Agrisure1 + Force 2.1G 0.12 T-band 404 12.6 b 3.1 b Pioneer-RR2 + Force 2.1G 0.12 T-band 435 15.2 b 3.5 b

1 Planted May 10, 2011; evaluated August 24, 2011 2 Ten corn ears per row in rows 2 and 7 of each plot were infested on August 10, 2011 with 5 2nd instar CEW larvae 3 Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 35F34PDR); Agrisure1 = Syngenta Agrisure GT hybrid (N53-W2); DeKalb-RR2 = DeKalb brand RR (DKC 55-08); Agrisure2 = Syngenta Agrisure 3111 hybrid (N53C-3111); Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 35F37) 4 Insecticide listed as ounces a.i. per 1,000 row-feet 5 T-band = Insecticide applied at planting time 6 Mean total kernels, damaged kernels counts and % damaged based on 40 observations (10 ears/treatment x 4 replications) 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Mean total kernels, damaged kernels counts and % damaged based on 40 observations (10 ears/treatment x 4 replications) 9 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

Table 44. Kernel area (cm2) consumed and number of total damaged ears for the Monsanto genuity smartstax

features and benefits study, Ames, IA.1,2

Kernel Area Total Treatment3 Form. Rate4 Placement5 Consumed (cm2)6,7 Damaged Ears 8,9

Agrisure2 ------ ------ ------- 0.00a 0a Smartstax ------ ------ ------ 0.00a 0a Pioneer-HXX ------ ------ ------ 1.34 b 8 b DeKalb-RR2 ------ ------ ------- 3.16 b 9 b DeKalb-RR2 + Force 2.1G 0.12 T-band 4.97 b 7 b Agrisure1 + Force 2.1G 0.12 T-band 5.05 b 7 b Pioneer-RR2 + Force 2.1G 0.12 T-band 5.50 b 10 b

1 Planted May 10, 2011; evaluated August 24, 2011 2 Ten corn ears per row in rows 2 and 7 of each plot were infested on August 10, 2011 with 5 2nd instar CEW larvae 3 Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 35F34PDR); Agrisure1 = Syngenta Agrisure GT hybrid (N53-W2); DeKalb-RR2 = DeKalb brand RR (DKC 55-08); Agrisure2 = Syngenta Agrisure 3111 hybrid (N53C-3111); Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 35F37) 4 Insecticide listed as ounces a.i. per 1,000 row-feet 5 T-band = Insecticide applied at planting time 6 Kernel area consumed based on 40 observations (10 ears/treatment x 4 replications) 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Total damaged ears based on 40 observations (10 ears/treatment x 4 replications) 9 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

34

APPENDIX I

Agronomic Information

35

2011 Field History Data

Ames, IA Crawfordsville, IA Corn rootworm studies Corn rootworm studies (following trap crop) (following trap crop) Insecticide History 2010 No Insecticide (trap crop) No Insecticide (trap crop) 2009 No Insecticide (soybeans) Insecticide Test Plot 2008 Insecticide Test Plot No Insecticide (trap crop) 2007 No Insecticide (trap crop) Insecticide Test Plot Tillage Fall-disk ripper;

Spring-field cultivation (2X) Fall-chiseled;

Spring-field cultivated Variety Pioneer P1395XR, Pioneer

TQ38148214, Pioneer P1395HR, Pioneer P0413R, DKC46-60, DKC44-92, DKC46-61, Mycogen 2K592, DKC61-36, DKC61-21, DKC62-97DKL, DKC61-21JRM, DKC61-97WTM, DKC62-97BPY

DKC61-21 & DKC61-72, Mycogen 2K591, Mycogen 2K592, Mycogen 2K594, Pioneer 35F34PDR, Agrisure N53C-3111, DKC55-09, DKC55-08, Agrisure N53-W2, Pioneer 35F37, DKC55-08

Planting Date(s) May 10 May 5 Planting Rate

35,600 seeds/A

35,600 seeds/A

Herbicide1 6 oz Balance + 32 oz Glystar-May 2;3 oz Capreno-June 18

2.5 qt Harness Extra + 3 oz Balance Flexx-May 7; 22 oz Roundup PowerMax + 1.25 qt Infantry 4L + 2.5 lbs AMS-June 7

Fertilizer2

N P K

N P K

Fall Applied --- --- --- --- --- --- Preplant

136 --- --- 250 --- ---

Dates Stand Count June 7, September 22 June 8, September 30 Root Digging August 10,11,12 August 9 Lodging September 21,22 September 30 Harvest October 12,13 October 7 Soil Type Clay Loam Silty Clay Loam Soil Organic Matter % 3.7 5.1 Soil pH 5.6 4.9

1 Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring-Preplant: 136 lbs actual nitrogen applied as urea on May 2 (Ames); Spring-Preplant 250 lbs actual nitrogen applied as anhydrous ammonia (82.5-0-0) on April 2 (Crawfordsville).

36


1 Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring-preplant 200 lbs actual nitrogen applied as anhydrous ammonia (82.5-0- 0) on April 3 (Nashua).

Nashua, IA Nashua, IA Corn rootworm studies Monsanto corn rootworm study (following trap crop) (following continuous corn) Insecticide History 2010 No Insecticide (trap crop) VT3 Hybrid Test Plot (Aztec) 2009 Insecticide Test Plot VT3 Hybrid Test Plot 2008 No Insecticide (trap crop) VT3 Hybrid Test Plot 2007 Insecticide Test Plot YIELDGARD + Hybrid Test Plot Tillage Fall-chopped stalks & chisel;

Spring-field cultivated Fall-chopped stalks & chisel; Spring-field cultivated

Variety DKC61-21 & DKC61-72,

Mycogen 2K591, Mycogen 2K592, Mycogen 2K594,

DKC46-60, DKC44-92 & DKC46-61; Mycogen 2K592

Planting Date(s)

May 4

May 4

Planting Rate

35,600 seeds/A

35,600 seeds/A

Herbicides1 2.3 qt Harness Extra-May 9;

2 pt Marksman-June 1 2.3 qt Harness Extra-May 9; 2 pt Marksman-June 1

Fertilizer2

N P K

N P K

Fall Applied --- --- --- --- --- --- Preplant 200 --- --- 200 --- --- Dates

Stand Count June 7 & September 23 June 7 & September 23 Root Digging August 1 August 1 Lodging September 23 September 23 Harvest

October 14 October 14

Soil Type Loam Loam Soil Organic Matter % 3.9 3.9 Soil pH 7.6 7.6

37


Northeast Iowa Ames, IA Monsanto corn rootworm study Pioneer ECB OAM II study (following continuous corn) (following soybeans) Insecticide History 2010 Corn No insecticide (SB’s) 2009 Corn (Insecticide applied) No insecticide (SB’s) 2008 Corn (Insecticide applied) No insecticide (SB’s) 2007 Corn (Insecticide applied) Soybeans (with Warrior) Tillage Fall-chopped stalks & chisel;

Spring-field cultivated Spring-field cultivation (2X)

Variety DKC46-60, DKC44-92 & DKC46-

61; Mycogen 2K592

Pioneer P0461HR, Pioneer P0461YHR, Pioneer P0461R

Planting Date

May 4

May 11

Planting Rate 35,600 seeds/A

35,600 seeds/A

Herbicides1 2.5 pts G-Max LiteTM + 1 qt Accomplish-April 6; 22 oz Roundup PowerMax + 1 oz Status + 2 oz Radiate-June 6

2.5 pts Harness-May 2; 3 oz Capreno-June 8.

Fungicides1 4 oz Headline-June 6; 9 oz Headline Amp-July 20.

None

Fertilizer2

N P K

N P K

Fall Applied 170 --- --- --- 60 90 Preplant 75 --- --- 136 --- --- Dates

Stand Count June 2 & September 16 June 7, 15 & 30, September 21 Root Digging August 5 N/A ECB Scoring - N/A July 15 Stalk Rating N/A October 6,7 Lodging September 16 September 21 Harvest October 4 October 12 Soil Type

Clay loam

Loam

Soil Organic Matter 3.5 4.1 Soil pH 6.5 5.8 1 Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring-preplant 136 lbs actual nitrogen (urea) applied on May 2 (Ames). Fall-2010 applied 60 lbs of Phosphorus and 90 lbs of potassium (Ames). Spring-preplant 75 lbs applied as 28% Nitrogen fertilizer on April 6 (Northeast Iowa). Fall-applied 170 lbs actual nitrogen applied as anhydrous ammonia (82.5-0- 0) in November 2010 (Northeast Iowa).

38


Ames, IA-Old Dairy Farm Ames, IA-South Dairy Farm Syngenta efficacy study Syngenta efficacy study (following corn) (following corn) Insecticide History

2010 Corn Corn 2009 Soybeans Corn 2008 Corn Soybeans (Warrior) 2007 Soybeans Corn

Tillage Fall-Chisel plow Spring-field cultivation (2X)

Fall-Disk ripper Spring-field cultivation (2X)

Variety Agrisure GT/CB/LL, Agrisure 3000GT, Agrisure Viptera 3111, Agrisure Viptera 3122, Agrisure 3122 Viptera EZ Refuge, Agrisure 3122 Viptera EZ Refuge2

Agrisure GT/CB/LL, Agrisure 3000GT, Agrisure Viptera 3111, Agrisure Viptera 3122, Agrisure 3122 Viptera EZ Refuge, Agrisure 3122 Viptera EZ Refuge2

Planting Date

May 18

May 19

Planting Rate 32,000 seeds/A 32,000 seeds/A

Herbicides1 28 oz Dual II Magnum-May 11; 3 oz Laudis-June 16.

28 oz Dual II Magnum-May 19; 6 oz Status-June 24.

Fertilizer2

N P K

N P K

Fall Applied --- --- --- --- --- --- Preplant 210 --- --- 210 --- --- Dates Stand Count June 8 & October 19 June 8 & October 20 Root Digging August 17 August 17 Lodging October 19 October 20 Harvest

October 19 October 20

Soil Type Loam Loam

Soil Organic Matter % 6.5 3.0 Soil pH 6.5 6.5 1 Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring-preplant 210 lbs actual nitrogen (urea) applied on May 10 at Old Dairy Farm (Ames). Spring-preplant 210 lbs actual nitrogen (urea) applied on May 16 at South Dairy Farm (Ames).

39


Ames, IA Ames, IA Dow AgroSciences CEW study Monsanto features & benefits study (following soybeans) (following trap crop) Insecticide History 2010 No insecticide (SB’s) No Insecticide (trap crop) 2009 No insecticide (corn) No Insecticide (soybeans) 2008 No insecticide (SB’s) Insecticide Test Plot 2007 No insecticide (corn) No Insecticide (trap crop) Tillage Spring-field cultivation (2X) Fall-disk ripper;

Spring-field cultivation (2X) Variety Mycogen 2K591, Mycogen

2K592, Mycogen 2K594 Pioneer 35F34PDR, Agrisure N53C-3111, DKC55-09, DKC55-08, Agrisure N53-W2, Pioneer 35F37, DKC55-08

Planting Date

May 10

May 10

Planting Rate 35,600 seeds/A 35,600 seeds/A

Herbicides1 2.5 pts Harness-May 2; 3 oz Capreno-June 8.

6 oz Balance + 32 oz Glystar-May 2; 3 oz Capreno-June 18

Fertilizer2

N P K

N P K

Fall Applied --- 60 90 --- --- --- Preplant 136 --- --- 136 --- --- Dates

Stand Count June 7 June 7 and September 22 CEW Infestations August 4 August 10 CEW Ear assessments

August 18 August 24

Soil Type

Loam

Clay Loam

Soil Organic Matter 4.1 3.7 Soil pH 5.8 5.6

1 Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring-preplant 136 lbs actual nitrogen (urea) applied on May 2. (Ames); Fall-2010 applied 60 lbs of Phosphorus and 90 lbs of potassium. (Ames-Dow Agro Sciences study only).

40

APPENDIX II

Weather Data

41

Ames1

Rainfall and Temperature 2011 May June July Temp (F) Rainfall Temp (F) Rainfall Temp (F) Rainfall

Day High Low Inches High Low Inches High Low Inches

1 58 33 81 49 80 73 0.24 2 54 27 82 65 77 71 0.08 3 59 26 77 68 75 70 4 53 43 74 68 76 68 5 56 48 0.01 75 66 0.02 80 62 6 58 46 99 70 84 62 7 60 52 100 73 86 63 8 76 50 87 60 78 70 9 77 57 66 54 1.56 78 73 10 99 59 64 62 0.42 89 72 0.50 11 82 64 0.28 66 61 84 68 0.26 12 67 60 0.23 68 52 0.04 72 60 0.02 13 61 54 0.23 72 54 0.31 77 57 14 54 51 0.11 73 60 0.12 79 62 15 64 42 0.31 75 57 0.13 78 73 16 66 36 85 54 80 75 0.03 17 57 48 73 66 92 77 18 72 39 71 68 94 74 19 75 48 83 62 93 76 20 63 59 0.90 78 62 0.47 92 74 21 66 61 0.21 72 62 0.21 81 76 0.43 22 74 57 0.01 69 60 0.03 80 75 0.26 23 76 55 64 55 79 75 0.08 24 78 55 0.43 67 60 82 61 0.02 25 61 49 0.90 66 62 0.13 86 56 26 64 44 70 63 0.98 88 69 27 57 54 69 64 87 75 28 62 55 70 63 86 70 0.48 29 62 57 0.05 73 65 80 74 30 90 67 79 70 80 73 31 79 58 0.05 88 70

Mean/Total 58.6 3.72 68.5 4.42 76.1 2.40

Normal 61.3 4.19 70.4 4.76 73.8 4.43 D.F.N. -2.70 -0.47 -1.90 -0.34 2.30 -2.03

1Weather station located 5 miles WSW of test site.

42

Ames1

Rainfall and Temperature 2011 August September October Temp (F) Rainfall Temp (F) Rainfall Temp (F) Rainfall


1 91 71 94 71 65 35 2 90 74 89 67 0.01 75 35 3 85 66 73 57 0.05 78 42 4 76 66 0.98 70 52 83 48 5 78 70 0.15 69 46 82 51 6 87 70 71 47 80 50 7 82 65 72 48 82 60 8 80 64 0.23 79 50 82 61 9 79 60 80 59 79 54 10 76 57 81 55 74 52 0.0111 80 58 85 56 76 47 12 82 63 88 58 67 54 0.5113 76 63 72 53 68 45 14 83 59 60 42 0.01 62 44 15 83 59 59 34 67 40 16 77 63 0.10 53 45 0.04 61 39 17 83 66 58 49 57 33 18 84 61 0.07 60 53 0.62 49 34 19 84 60 76 54 0.01 48 33 20 79 62 75 51 0.03 54 31 21 81 62 65 48 65 27 22 84 63 61 41 65 42 23 90 70 66 39 74 39 24 85 60 65 38 73 38 25 83 56 66 39 76 45 26 80 60 66 49 53 40 27 82 61 68 52 55 33 28 77 57 82 54 56 30 29 80 56 72 54 57 27 30 66 62 0.03 67 43 55 37 31 86 61 57 28

Mean/Total 72.2 1.56 60.8 0.77 54.1 0.52 Normal 71.5 4.55 64.2 3.00 52.2 2.37 D.F.N. 0.07 -2.99 -3.40 -2.23 1.9 -1.85

1Weather station located 5 miles WSW of test site.

43

Crawfordsville1



1 61 40 85 53 0.03 95 76 2 59 34 71 61 0.40 89 67 3 54 30 92 65 0.33 83 64 4 64 26 88 66 88 61 5 63 44 87 63 1.34 86 60 6 72 40 0.05 95 68 90 63 7 79 52 94 71 84 65 8 78 48 92 69 91 63 9 85 59 70 57 0.04 90 63

10 94 62 0.10 72 53 1.22 93 69 11 89 65 69 55 0.09 88 70 12 86 59 72 51 85 65 13 66 53 74 55 80 64 0.03 14 53 48 0.69 75 57 1.04 85 60 15 65 42 0.97 78 61 2.05 89 67 16 64 35 0.01 85 56 90 71 17 68 34 82 62 94 72 18 72 39 78 63 96 78 19 77 48 85 65 99 78 20 82 57 82 66 0.37 97 76 21 82 62 0.39 83 65 0.17 89 71 22 82 57 0.38 69 62 86 68 0.67 23 79 62 0.23 68 60 84 70 24 84 58 75 57 84 68 0.31 25 66 50 0.77 70 56 86 63 0.04 26 66 46 1.19 82 58 0.35 90 68 27 65 42 83 62 0.74 90 73 28 72 53 81 56 86 70 0.26 29 77 53 84 56 85 67 30 90 62 0.87 94 65 87 65 31 84 62 90 65

Mean/Total 61.3 5.65 70.5 8.17 78.2 1.31 Normal 61.5 5.15 70.8 5.37 73.7 3.33 D.F.N. -0.02 0.50 -0.03 2.80 4.5 -2.02

1Weather station located at test site.

44

Crawfordsville1



1 91 68 93 69 63 32 2 92 74 88 70 73 31 3 86 64 74 59 80 37 4 81 62 70 52 1.19 82 42 5 82 68 69 44 84 44 6 86 69 70 42 84 45 7 85 65 0.31 72 43 86 52 8 79 62 79 49 85 53 9 79 60 79 53 83 48

10 76 54 81 52 80 45 11 81 51 85 52 77 53 0.23 12 82 58 87 56 68 50 13 76 61 72 50 69 49 0.30 14 81 56 61 39 0.06 60 46 15 81 55 59 34 66 40 16 79 58 53 43 60 40 17 84 59 62 46 55 33 18 84 55 61 56 52 39 19 86 57 75 47 35 33 20 80 62 77 45 0.27 36 32 21 81 56 65 48 61 28 22 84 55 62 40 68 33 23 87 67 63 36 78 36 24 85 61 63 34 70 37 0.06 25 83 56 65 39 78 50 26 81 55 61 48 52 42 27 82 60 66 50 0.25 56 30 28 76 55 71 49 59 25 29 80 53 71 49 58 27 30 69 59 66 42 55 34 31 85 61 0.73 54 31

Mean/Total 70.9 1.04 59.4 1.77 52.9 0.59Normal 72.2 5.01 64.8 3.21 51.9 3.29D.F.N. -1.3 -3.97 5.4 -1.44 1.0 -2.70


45

Northeast Iowa1



1 68 38 T 81 51 0.01 92 70 T 2 58 28 82 55 95 70 3 54 29 68 58 0.08 86 58 4 51 24 90 67 80 55 5 62 29 T 87 59 86 56 6 64 37 T 88 65 84 62 T 7 72 44 0.31 96 69 88 64 T 8 66 40 94 73 T 84 61 T 9 73 56 93 60 0.08 88 63

10 71 59 0.02 65 52 1.68 87 68 11 91 64 68 55 T 89 67 1.31 12 87 61 0.01 69 47 0.02 85 64 0.04 13 85 54 0.03 71 51 0.01 80 59 0.12 14 57 45 0.19 72 57 T 78 57 15 50 45 0.20 75 59 1.14 78 59 T 16 65 34 T 74 56 0.33 84 69 0.18 17 62 30 83 59 89 71 0.48 18 66 35 83 63 0.03 93 77 19 65 44 T 78 65 0.05 95 77 20 74 53 84 67 0.01 97 73 21 77 59 T 79 64 0.39 95 76 22 81 57 0.24 81 63 0.23 85 66 0.65 23 77 60 T 67 58 0.07 86 67 3.72 24 78 51 64 56 84 70 0.73 25 80 49 0.65 73 56 86 61 T 26 54 45 0.19 69 54 85 63 27 63 38 79 61 88 72 28 62 44 0.04 81 55 90 70 2.37 29 70 47 79 53 82 68 0.49 30 74 53 0.28 82 61 87 65 31 87 69 0.01 87 64

Mean/Total 57.5 2.17 68.7 4.16 76.4 10.09 Normal 58.0 4.32 67.9 5.17 71.7 4.91 D.F.N. -0.05 -2.15 0.08 -1.01 4.7 5.18

1Closest weather station located in Manchester, Iowa, approximately 20 miles West Northwest.

46

Northeast Iowa1



1 90 69 84 62 63 30 2 90 71 92 71 0.08 63 29 3 92 71 87 67 0.27 72 29 4 85 61 74 52 0.35 78 37 5 82 65 T 68 46 79 40 6 83 67 0.02 66 40 81 43 7 85 68 68 41 80 44 8 85 61 71 41 83 57 9 75 62 0.32 77 49 83 48

10 78 53 76 50 79 44 11 76 49 79 51 77 44 0.13 12 80 50 83 58 74 49 T 13 81 61 0.01 85 53 64 50 0.76 14 75 55 0.08 70 44 T 66 45 T 15 80 53 59 32 59 41 16 81 55 58 33 62 41 0.11 17 82 58 51 41 0.07 60 35 18 83 51 64 46 55 35 19 83 55 59 56 0.14 48 35 20 87 57 0.05 73 44 48 35 21 81 56 0.01 79 46 T 53 27 22 79 53 64 47 T 59 27 23 84 53 0.47 59 39 0.08 65 29 24 83 66 0.53 57 35 68 30 0.11 25 84 54 61 39 0.10 67 31 0.02 26 81 53 62 47 0.10 73 42 27 82 54 59 47 0.50 49 38 T 28 79 53 66 47 0.19 53 24 T 29 76 53 0.03 64 46 0.02 58 23 T 30 80 54 69 46 0.01 54 23 31 68 59 1.28 52 34 0.14

Mean/Total 69.8 2.80 58.3 1.91 51.0 1.27Normal 69.5 4.84 60.9 3.30 48.9 2.85D.F.N. 0.03 -2.04 -2.6 -1.39 2.1 -1.58

1Closest weather station located in Manchester, Iowa, approximately 20 miles West Northwest.

47

Nashua1



1 57 35 82 50 94 71 0.02 2 49 28 72 60 0.13 85 64 3 56 32 T 95 64 79 61 4 63 29 T 87 67 0.01 85 56 5 66 46 89 62 80 61 T 6 72 38 0.09 97 69 85 63 7 69 47 97 69 86 63 T 8 74 44 91 63 89 61 9 68 54 0.18 66 55 0.74 86 65

10 93 61 61 53 0.43 89 70 0.03 11 81 63 0.05 69 52 85 69 0.20 12 82 51 67 49 T 74 60 0.08 13 57 50 0.14 72 53 78 56 14 50 43 0.31 73 58 0.20 79 59 0.08 15 63 43 0.30 74 59 1.43 87 68 1.67 16 64 38 84 57 89 70 17 66 40 85 62 92 76 18 70 39 81 63 94 76 19 73 51 83 66 96 77 20 75 58 0.37 77 66 0.24 93 74 21 77 59 0.44 78 63 0.77 80 68 0.07 22 75 57 0.18 72 61 0.65 82 69 0.31 23 78 56 63 57 0.08 86 71 0.64 24 77 54 0.05 74 52 83 64 25 57 49 1.22 72 57 T 85 58 26 64 45 81 56 87 70 T 27 60 44 0.04 78 58 0.07 87 74 28 74 52 79 52 82 69 0.38 29 65 52 0.01 82 57 87 66 30 89 64 94 66 87 65 31 81 59 0.01 89 67

Mean/Total 58.5 3.84 69.2 4.75 76.2 3.48 Normal 59.2 4.49 68.5 5.17 71.9 4.81 D.F.N. -0.07 -0.65 0.07 -0.42 4.3 -1.33


48

Nashua1



1 90 70 92 69 63 34 2 90 73 82 68 0.30 74 34 3 84 63 75 54 0.84 79 41 4 81 61 67 48 80 45 5 83 68 68 43 83 51 6 84 66 T 69 43 80 50 7 83 64 0.13 72 43 83 61 8 79 62 0.20 79 49 82 61 9 77 55 79 55 80 51

10 76 52 81 51 76 50 11 81 50 86 53 76 53 12 81 60 86 57 66 54 13 74 60 1.95 69 50 63 46 14 81 56 58 37 58 44 15 81 57 58 32 61 38 16 81 58 53 45 60 36 17 80 58 62 48 0.01 58 33 0.17 18 84 55 T 59 55 0.67 45 33 19 86 57 73 49 49 39 1.26 20 77 60 0.31 74 49 0.04 54 30 21 80 54 61 50 T 61 26 22 84 55 58 46 63 37 0.02 23 86 66 1.43 61 41 69 36 24 80 57 60 36 71 34 25 83 55 63 42 74 44 26 82 58 59 50 0.46 51 41 27 79 57 66 51 T 53 33 28 74 54 74 52 55 28 29 80 52 69 51 55 23 30 65 61 0.58 64 40 52 37 0.07 31 84 64 52 33

Mean/Total 70.1 4.60 59.0 2.32 52.9 1.52 Normal 69.7 4.31 62.0 3.11 49.2 2.63 D.F.N. 0.04 0.29 -3.0 -0.79 3.7 -1.11


49

APPENDIX III

Materials Tested

50

Materials Tested in 2011 Iowa State University Efficacy Studies

Common/code name Formulation Chemical name Company

Aztec 2.1G tebupirimphos & cyfluthrin AMVAC Chem. Corp.

Counter 20G terbufos AMVAC Chem. Corp.

Cruiser Commercially applied seed trt (0.25 mg/sd) thiamethoxam Syngenta

Cruiser Extreme 250 Commercially applied seed trt (0.25 mg/sd)

thiamethoxam+ three fungicides (Maxim XL+Apron XL and Dynasty) Syngenta

DKC44-92

RR2 + commercially applied Poncho 500/VOTIVO

Roundup Ready 2 Corn + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.50 mg/seed) + Bacillus firmus I-1582 Monsanto

DKC46-60 (YieldGard VT Triple with commercial seed trt)

Transgenic seedcorn (YGRW+YGCB+RR2) + commercially applied Poncho 500/VOTIVO

Cry3Bb1 (MON 863) + Cry1Ab (MON810) + Roundup Ready Corn 2 + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.50 mg/seed) + Bacillus firmus I-1582 Monsanto

DKC46-61 (Smartstax with commercial seed trt)

Transgenic seedcorn (CRW/RR2(VT)) +BT (VT)+CRW (HXRW)+BT (HX1) commercially applied Poncho 500/VOTIVO

Cry3Bb1 & RR2 (MON 88017) + Cry1A.105&Cry2Ab2(MON89034) +Cry34Ab1&Cry35Ab1(DAS59122) +Cry1F&LL(TC1507) + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.25 mg/seed) + Bacillus firmus I-1582 Monsanto

DKC55-08









DKC61-21JRM (Smartstax RIB (95%Bt/5%non-Bt) with commercial seed trt)



DKC61-36



DKC61-72

DKC61-72 (RR2) ‘’true isoline seed” of DKC61-69 + commercially applied Poncho 500/VOTIVO


DKC61-97DKL VT3P RIB (95%Bt/5%non-Bt) with commercial seed trt

Transgenic seedcorn (YGRW+YGCB+RR2)+ commercially applied Poncho 500/VOTIVO

Cry3Bb1 (MON 863) + Cry1A.105/Cry2Ab2 (MON89034) + Roundup Ready 2 Corn + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.50 mg/seed) + Bacillus firmus I-1582 Monsanto

51

Materials Tested for 2011 (Continued)

Common/code name Formulation Chemical name Company

DKC61-97WTM VT3P RIB (100%Bt) with commercial seed trt

Transgenic seedcorn (YGRW+YGCB) + commercially applied Poncho 500/VOTIVO

Cry3Bb1 (MON 863) + Cry1A.105/Cry2Ab2 (MON89034) + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.50 mg/seed) + Bacillus firmus I-1582 Monsanto

DKC61-97BPY VT3P RIB (80%Bt/20%non-Bt) with commercial seed trt

Transgenic seedcorn (YGRW+YGCB+RR2)+ commercially applied Poncho 500/VOTIVO

Cry3Bb1 (MON 863) + Cry1A.105/Cry2Ab2 (MON89034) + Roundup Ready 2 Corn + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.50 mg/seed) + Bacillus firmus I-1582 Monsanto

Force 3G tefluthrin Syngenta Crop Protection

Mycogen 2K591

Mycogen 2K591 “true isoline seed” of Mycogen 2K592

Roundup Ready 2 Corn + Thiamethoxam seed trt (0.25 mg/seed) Dow AgroSciences

Mycogen 2K592 (Herculex/RR) with Cruiser Extreme 250 seed trt Dow Background

Cry34AB1 & Cry35Ab1 + Cry1F + RR2 + thiamethoxam seed trt (0.25 mg/seed) Dow AgroSciences

Mycogen 2K594 (Smartstax) with Cruiser Extreme 250 seed trt Dow Background

Cry34AB1 & Cry35Ab1 + Cry1F + Cry38b1 + Cry1A.105 + Cry 2Ab2 + RR2 + Liberty Link + thiamethoxam seed trt (0.25 mg/seed) Dow Background

Mycogen 2K594+2K591 (RIB) with Cruiser Extreme 250 seed trt Dow Background

Cry34AB1 & Cry35Ab1 + Cry1F + Cry38b1 + Cry1A.105 + Cry 2Ab2 + RR2 + Liberty Link + thiamethoxam seed trt (0.25 mg/seed) Dow Background

Pioneer P0413R (RR2) with Poncho 1250/VOTIVO seed treatment Pioneer Background

Roundup Ready Corn 2 + mefenoxam fungicide + clothianidin seed trt (1.25 mg/seed) + Bacillus firmus I-1582

Pioneer Hi-Bred International, Inc

Pioneer P0413R (RR2) with Cruiser 250 seed treatment Pioneer Background

Roundup Ready 2 Corn + mefenoxam fungicide + thiamethoxam seed trt (0.25 mg/seed)


Pioneer TQ38148214 (pyramid) with Cruiser 250 seed treatment Pioneer Background

Cry34Ab1 & Cry35Ab1 + Cry1F + Cry1Ab + MIR604 + mefenoxam fungicide + thiamethoxam seed trt (0.25 mg/seed)


Pioneer P1395HR (HX1) with Cruiser 250 seed treatment

Pioneer P1395HR “near isoline seed” of Pioneer P1395XR

mefenoxam fungicide + Clothianidin + thiamethoxam seed trt (0.25 mg/seed)


Pioneer P1395XR (Herculex XTRA) with Cruiser 250 seed treatment Pioneer Background

Cry34AB1 & Cry35Ab1 + Cry1F + mefenoxam fungicide + thiamethoxam seed trt (0.25 mg/seed)

Pioneer Hi-Bred International, Inc.

Pioneer P0461HR (HX1) with Cruiser 250 seed treatment

Pioneer P0461HR “near isoline seed” of Pioneer P0461R

mefenoxam fungicide + Clothianidin + thiamethoxam seed trt (0.25 mg/seed)


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Materials Tested for 2011 (Continued)

Common/code name Formulation Chemical Name Company Pioneer P0461R (RR2) with Cruiser 250 seed treatment Pioneer Background



Pioneer 35F34PDR (Herculex XTRA) with Cruiser 250 seed treatment Pioneer Background

Cry34AB1 & Cry35Ab1 + Cry1F + mefenoxam fungicide + thiamethoxam seed trt (0.25 mg/seed)


Pioneer 35F37 (RR2) with Cruiser 250 seed treatment Pioneer Background



Poncho 250 Commercially applied seed trt (0.25 mg/seed) clothianidin Bayer CropScience



SmartChoice 5G Chlorethoxyfos AMVAC Chem. Corp.

Syngenta Agrisure 3111 hybrid (N53C-3111)

Transgenic seedcorn Bt11+MIR604+GA21

Cry1Ab (Bt11) + mCry3A (MIR604) + mEPSPS (GA21)

Syngenta Crop Protection

Syngenta Agrisure GT hybrid (N53-W2) GA21 mEPSPS (GA21)

Syngenta Crop Protection

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APPENDIX IV

Research Pictures

2011 iowa evaluation of insecticides and plant ... · 2011 iowa evaluation of insecticides and...

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