2014_chr hansen_cheese culture catalogue

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    Cheese Culture Catalogue

    DVS® Product Range 

    2014

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     DVS® Benefits

    • Yield Plus™

    • Risk Minus™

    • Growth Multiply™

    • Process Convenience

    • Chr. Hansen Expertise &

      Competencies

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    Imagine a world of opportunities just waiting to be grasped

    To all of those who are fearful that Chr. Hansen DVS® cheese cultures mean standardization, banality

    and leveling-down, this catalogue should bring some comfort. Chr. Hansen has the broadest, most

    extensive culture range in the dairy world and combines tradition, innovation, loyalty and change -

    imagine where we could go together.

    A winning culture for all types

    Since the 1970’s – when Chr. Hansen invented highly concentrated and standardized cheese cultures as

    for example the Direct Vat Set (DVS®)  –  we have focused on reliability and quality at the forefront of

    market trends. Chr. Hansen provides today’s dairy industry with a full pallet of Cultures & Enzymes

    solutions. This is delivered together with application know-how, service and inspiration.

    Do you know how many cheeses there are?

    We do not know  – but there are probably several thousands. In order to simplify the navigation in this

    complex cheese world, we have classified our culture series and defined eight groups of cheese “families”as well as more specialized cultures. Together, these segments form the backbone of this catalogue.

    Value-creating innovation and local support

    Today, our most valuable commodity is know-how within application and process control. We have more

    than 20 application centers around the world providing you with the opportunity to carry out trials with

    new and existing Chr. Hansen products, in a production environment similar to your facilities at home.

    For further information, please contact your local sales representative or visit us at www.chr-hansen.com

    ConsumerChr. HansenCheese

    Manufacturer

    Innovative

    DVS® 

    Unlimited

    cheeses 

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    Table of Content

    DVS® & EASY-SET® Benefits Page 1

    Cultures for all Cheese Segments Page 2

    Cultures for Special Purposes Page 3

    Types of Microorganisms in Cheese Cultures Page 4

    The Magic of Cultures Page 6

    Distinguishing Characteristics of Lactic Acid Bacteria Page 9

    Information on Direct Vat Set/DVS® Page 10

    Cheddar Cheese Cultures Page 11

    Continental Cheese Cultures Page 14

    Cottage Cheese Cultures Page 19

    Pasta Filata Cheese Cultures Page 22

    Soft Cheese Cultures Page 25

    Propionic Cheese Cultures Page 30

    Grana Cheese Cultures Page 34

    White Brined Cheese Cultures Page 37

    Ripening Cultures Page 40

    Protective Cultures Page 48

    Kosher For Passover Page 50

    Our Product Range & How to use it Page 51

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    1

     

    DVS® & EASY-SET® Benefits

    Yield PlusTM

     

    Your booster to get more value –

     you can focus onoptimizing yield without worrying about the consistency

    of your cheese quality

      Capture high-value niche opportunities or extracting the

    value from whey

      No added investments in infrastructure required

    Risk MinusTM

      Your insurance policy to secure consistent delivery

      Phage management and backup solutions – Robustness

    to Phages  Expand internationally or maybe consolidate plants – 

    minimizing risk

    Growth MultiplyTM

       Your kick-starter to innovation and new business

    opportunities e.g. products and processes

      Proven capabilities and innovative methods focusing on

    customer needs

     

    Full pallet of culture solutions and ability to customize

    Process convenience  Easy to use: No preparation

      Easy to transport

      Flexibility in production planning (and cycle) 

    Chr Hansen Expertise & Competencies  Global network and active knowledge sharing

      Global supply and presence

      Value creating innovation

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    2

     

    Cultures for all Cheese Segments

    To simplify the process of identifying the best Chr. Hansen culture series to the cheese type being produced, this brochure is divided into eight

    main segments of “cheese families”. The table below provides an overview of the different DVS® culture series that are available.

    Cheese Segment Cheese types Chr. Hansen DVS®Cheddar, Monterey Jack, Colby,

    Territorials, American Cheddar

    and other types with no eyes and

    strong acidification.

    DVS® R

    DVS® RST

    DVS® RSF

    Semi-hard cheese types with

    small to medium size holes like:

    Gouda, Edam, Danbo, Samsø,

    Havarti, Saint Paulin, Raclette,

    Manchego, Prato etc.

    DVS® Flora Danica

    DVS® CH-N

    DVS® DCC

    EASY-SET® FLORA™  

    DVS® R

    Mozzarella, Pizza Cheese,

    Provolone and Kashkaval.

    DVS® STI

    DVS® TCC

    Cottage Cheese types Fresco®

    DVS® CC

    DVS® R

    Emmental, Gruyére, Maasdam  DVS® CH-N

    DVS® DCC

    DVS® STEM

    DVS® PS

    DVS® LH

    Camembert, Brie, Gorgonzola,

    Crezenza, Argentine Port Salut  

    DVS® CH-N/FLORA

    DVS® Mild O

    DVS® SDMB

    DVS® ST-B/SSC

    DVS® CZ

    DVS® BA

    Grana, Parmasan, Sbrinz,

    Pecorino

    DVS® Grana

    DVS® MLC

    DVS® TCC

    Feta and White brined cheeses DVS® SafeIT

    DVS® FRC

    DVS® Mild O

    DVS® R

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    3

     

    Cultures for Special Purposes 

    In addition to the eight cheese “families”, the culture cheese range also contains culture types – Ripening, Protective cultures, Kosher For

    Passover and Butter - which are used widely across many of the cheese segments for unique flavor development, extended shelf life etc.

    Culture types Cheese types Chr. Hansen culture seriesVarious cheese types when special flavor

    development during the ripening time is

    desired. Also certain mold and rind ripened

    cheese types.

    Flavor development:

    DVS® CR

    DVS® PS

    DVS® LH

    DVS® EMFOUR

    DVS® CR-Full Flavor

    Molds, Yeast, and Brevibacterium:

    SWING® SALSA, PC, PR/PRG, GEO, LAF, BL/BC

    Various cheese types when special shelf life

     properties are desired. 

    DVS® FreshQ®

    DVS® BioSafe™  

    Various cheese types when the Kosher For

    Passover certification is required.

     All Chr. Hansen cheese cultures are Kosher

    certified. These cultures also covers the special

     period of Kosher for Passover  

    KFP™ 

    Various butter and spread types both

    traditional and continuous processed

    DVS® D-series

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    4

     

    Types of Microorganisms in Cheese Cultures

    Culture Type Name Characteristics

    Lactococcus lactis subsp. cremorisLactococcus lactis subsp. lactis

    MesophilicHomofermentative

    Flavor contribution

    Leuconostoc sp. Mesophilic

    Heterofermentative

    Gas production 

    Lactococcus lactis subsp. lactis biovar diacetylactis Mesophilic

    Homofermentative

    Flavor contribution

    Gas production from citrate 

    Lactococcus lactis subsp. cremoris

    Lactococcus lactis subsp. lactis

    Leuconstoc sp. 

    Lactococcus lactis subsp. lactis biovar diacetylactis

    Mesophilic

    Heterofermentative

    Flavor contribution

    Gas production

    Streptococcus thermophilus  Thermophilic

    Homofermentative 

    Lactobacillus helveticus Thermophilic

    Homofermentative

    Flavor contribution

    Lactobacillus delbrueckii subsp. bulgaricus Thermophilic

    Homofermentative

    Flavor contribution

    Lactobacillus paracasei Mesophilic

    Fac. Heterofermentative

    Flavor contribution

    Lactobacillus delbrueckii subsp. lactis Thermophilic

    Homofermentative

    Flavor contribution

    Lactobacillus rhamnosus Thermophilic

    Fac. Heterofermentative

    Flavor contribution

    Lactobacillus curvatus Mesophilic

    Fac. Heterofermentative

    Flavor contribution 

    LD 

    ST 

    LbH 

    LbB 

    LbP 

    LbL 

    Lb rh 

    Lb cu 

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    5

     

    Culture Type Name Characteristics

    Lactobacillus plantarum MesophilicFac. Heterofermentative

    Flavor contribution

    Lactobacillus johnsonii Thermophilic

    Homofermentative

    Flavor contribution

    Pediococcus acidilactici Thermophilic

    Homofermentative

    Flavor contribution

    Penicillium candidum Surface yeast

    Flavor contribution

    White to grayish color tones

    Penicillium roqueforti Core yeast

    Flavor contribution

    Green to bluish color tones

    Geotrichum candidum Surface yeast

    Flavor contribution 

    Yeast Surface yeast

    Flavor contribution

     pH neutralizing and/or fermenting

     properties

    Staphylococcus xylosus  Flavor contribution

    Brevibacterium Smearing bacteria

    Flavor contributionSurface coating

    Orange color tones

    Propionibacterium Thermophilic

    Heterofermentative

    High gas production

    Lb pl 

    Lb jo 

    P ac 

    PC 

    PR 

    GEO 

    LAF 

    SALSA 

    BL 

    PAB 

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    6

     

    The Magic of Cultures

    Definitions of Culture Performance Rating

    The cultures in this catalogue are classified by their performance and functionality for:  Acidification

    speed , Phage robustness, Contribution to flavor , and when relevant also Gas production. The scale is a 5-

    level scale from low/slow to high/fast. As an example the below DCC series is rated for acidification

    speed (very fast), phage robustness (very robust), flavor (a real flavor maker) and also gas producing

    (giving many eyes in the cheese) - all indicated with black boxes for score. For example:

    DVS® DCC

    Low High

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Design of cultures

    Chr. Hansen’s sophisticated design technologies secure optimization of cultures , ensuring superior

    performance, phage robustness and quality of the final cheese. This catalogue highlights how we have

    constructed our cultures to maximize cheese manufacturer and consumer benefits. As illustrated in this

    catalogue, individual strains of bacteria are compounded together into high value cultures, allowing the

    manufacturer to produce almost unlimited types of cheese.

    APPLICATION TECHNOLOGY - STRAINS & CULTURE COMPOUNDING – 

     FOOD SAFETY

    3 SPECIAL GROUPS; PROTECTIVE - RIPENING –  KOSHER FOR PASSOVER

    8 CHEESE GROUPS OF DEDICATED DVS® CULTURE SOLUTIONS

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    7

     

    Mesophilic aromatic cultures 

    LD cultures are the classical aromatic starters containing all four species of mesophilic lactic acid bacteria:

      Lactococcus lactis subsp. lactis (O)

      Lactococcus lactis subsp. cremoris (O)

      Leuconostoc mesenteroides subsp. cremoris (L)

     

    Lactococcus lactis subsp. lactis biovar diacetylactis (D)

    LD cultures are mixtures of the culture components O, L, and D in various ratios. The term LD culture indicates

    the presence of the two aroma-forming bacteria, Leuconostoc and L. diacetylactis, which are characterized by

    their ability to produce aroma and gas by converting the citrate in milk into the flavor compound diacetyl and

    CO2.

    LD cultures are widely used in the production of soft cheeses like traditional Camembert and in continental

    cheeses with round eyes e.g. Gouda cheese. When producing the continental cheese types, one of the quality

    features is the right holes or "eye" formation. The size and the number of eyes in the cheese are some of the

    most difficult things to control. To a certain degree this eye formation is dependent on the rate of citrate

    fermentation in the cheese (measured as mg of citrate fermented after 24 hours). The composition betweenacid producing strains and aroma-forming strains as well as the ratio between L. cremoris and L. diacetylactis, 

    can be related to the rate of metabolism of citrate. However, many non-culture related factors are also

    involved e.g. production process, temperature, salt content, etc. The best known Chr. Hansen mesophilic

    aromatic cultures are the CH-N, Flora Danica, DCC and EASY-SET® FLORA series. 

    L cultures and D cultures, which only consist of either Leuconostoc or L. diacetylactis as the aroma-forming

    bacteria, are only used in cheese production to a limited extent. This is due to the traditional use of LD

    cultures in continental cheese production. Recent developments with Chr. Hansen has created a new Edam

    EASY-SET® FLORA C100 and C900 range which is a L culture blend of both L and O components, ideal for Edam

    manufacturing and other cheese types when a cheese with small eyes and traditional cheese flavour is

    desired. 

    Mesophilic homofermentative cultures – O culture 

    O cultures only consist of the acid-producing strains, Lactococcus lactis subsp.  lactis/cremoris. This type of

    culture is mainly used in Cheddar, Cottage Cheese, White Brined Cheese (Feta) and other cheese types in

    which a gas formation in the product is undesirable. The best known Chr. Hansen mesophilic

    homofermentative cultures are the R, FRC, and Mild O series.

    To improve the acidification activity of the O-type cultures for Cheddar and other fast cheese productions, a

    very fast series of cultures have been developed by blending mesophilic homofermentative cultures with S.

    thermophilus. During a typical Cheddar production the O culture will initiate the acidification during renneting

    at 30-32°C (86-90°F). However, due to the thermophilic nature of the S. thermophilus culture, this culture will

    take over the acidification during the scalding step at 37-40°C (98-104°F), and continue on the belt during thecheddaring process allowing the manufacturer to reach target pH quickly.

    Thermophilic cultures – ST culture - are mainly used in cheese in which either

      a scalding temperature 40-54°C (104-130°F) is used e.g. in hard cheese like Emmenthaler, Gruyere

    and Grana, or  

      a high scalding temperature during cheese make is used, e.g. 37°C (98°F), as in the soft cheese types

    like Cresenza and stabilised Brie types, or in the Pasta filata types like Mozzeralla and Pizza cheese.

    For Emmenthaler and Gruyere types of cheeses, S. thermophilus  and L. helveticus are normally used. L.

    helveticus is able to ferment the galactose which is secreted by the S. thermophilus,  and processes a high

    proteolytic activity, giving the required proteolysis and desired flavor.

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    8

     

    In production of Pasta filata types of cheese as well as different US style pizza cheeses, S. thermophilus is used

    as the main culture - sometimes in a blend with either L. bulgaricus or  L. helveticus  to obtain the right

    characteristics of the cheese. For production of Pasta Filata it is important to have a very fast acidifying and

    phage resistant culture. Examples of Chr. Hansen cultures for this application are STI, TCC, and TCC-20.

    Propionic acid bacteria may not be classified as real lactic acid bacteria, but they are used in the production ofcertain cheeses (Emmenthaler, Swiss, Maasdam, etc.), in which large eyes and sweet nutty flavors are

    desirable. Examples of Chr. Hansen cultures for this application are the PS series.

    Flavor Control™ cultures are regarded as a "package" of bacteria added as adjunct cultures, containing the

    right enzymes to modify the ripening process incl. foil ripening/rindless cheese. During ripening the additional

    bacteria added will lyses, releasing the intracellular enzymes into the cheese matrix and in this way modify the

    ripening process. Examples of Chr. Hansen cultures are CR-200, CR-300, and CR Full Flavor series.

    SWING® cultures are a range of aerobic cultures which can be divided into four main categories: Mold, Yeast,

    Brevibacterium and others. Due to the aerobic nature of the SWING® cultures, they mainly grow on the

    surface of cheese.

    The SWING® Mold cultures are mainly used in the production of white mold cheeses like Brie, Camembert

    and blue mold cheeses like Gorgonzola. The two most important types of mold are the white mold Penicillium

    candidum and the blue mold Penicillium roqueforti . SWING® Yeast  is often used to stimulate the growth of

    other microorganisms as Brevibacterium on the cheese surface, due to their ability to increase pH by

    fermenting lactic acid.

    SWING® Brevibacterium  (or the so-called "smear cultures") are traditionally smeared on the surface of

    cheese just after brining. Brevibacterium are very salt tolerant and have a strong proteolytic activity. During

    growth on the cheese surface, Brevibacterium linens creates a characteristic orange color and smear layer.

    Due to the high protein degradation on the cheese surface, flavor compounds penetrate into the centre of the

    cheese and give the characteristic ripened flavor of smear cheeses.

    We are pleased to present this overview of our cultures where innovation and responsiveness to market go

    hand in hand.

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    9

     

    Distinguishing Characteristics of Lactic Acid Bacteria

    Schematic

    illustration

    Organism Streptococcsthermophilus 

    Lactococcus

    lactis subsp. 

    cremoris

    Lactococcus

    lactis subsp. 

    lactis

    Lactococcus

    lactis subsp.

    Lactis biovar

    diacetylactis

    Leuconostoc

    sp. 

    Lactobacillus

    helveticus

    Lactobacillus

    delbrueckii

    subsp. 

    bulgaricus

    Lactobacillus

    delbrueckii

    subsp. Lactis

    Shape Cocci Cocci Cocci Cocci Cocci Rod Rod Rod

    Isomer of

    Lactate

    L L L L D DL D D

    % Lactic Acid

    Produced in

    Milk

    0.6 0.8 0.8 0.8 < 0.5 2.0 1.8 1.8

    Metabolism

    of Citrate

    (Flavor)

    - - - + + - - -

       G   r   o   w   t

        h   a   t

    10°C

    (50°F)- + + + + - - -

    40°C

    (104°F)

    + - + + - + + +

    45°C

    (113°F)+ - - - - + + +

       F   e   r   m

       e   n   t   a   t   i   o   n   o    f

    Glucose + + + + + + + +

    Galactose - + + + + + - +/-

    Lactose + + + + + + + +

    ST 

    LbH 

    LbB 

    LbL 

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    10

     

    Information on Direct Vat Set/DVS®

    DESCRIPTION

    DVS® is a highly concentrated and standardized

    frozen or freeze-dried dairy culture, used for

    direct inoculation of milk. DVS® cultures need

    no activation or other treatment prior to use. 

    APPEARANCE

    Frozen DVS® cultures are water-soluble

    pellets, approximately 5 mm in diameter,

    with an off-white to brownish color. The

    pellets have a slight peptone-like odor.

    Freeze-dried DVS® cultures are water-

    soluble granules, 2-8 mm in diameter, with

    an off-white to slightly red or brown color.

    The granules have a slight peptone-like odor.

    TECHNICAL DATA

    Chr. Hansen's DVS® cultures rely only on

    lactic acid bacteria, internationally approvedfor the dairy industry. Our cultures are all of

    natural origin and are produced using the

    latest production technology.

    PURITY

    Chr. Hansen's DVS® cultures comply with IDF

    149 (International Dairy Federation) / ISO

    27205:2010 standards, regarding maximum

    contaminant content.

    Frozen

    DVS®

    Freeze-

    dried

    DVS®

    Non lactic

    acid bacteria < 500cfu/g

    < 500cfu/g

    Yeasts and molds < 1 cfu/g < 10 cfu/g

    Enterobacteriaceae < 1 cfu/g < 10 cfu/g

    Coagulase-positive

    staphylococci < 1 cfu/g < 10 cfu/g

    Salmonella spp.* absent in

    25g

    absent in

    25g

    Listeria

    monocytogenes*

    absent in

    25g

    absent in

    25g

    * analyzed on a regular basis  

    STORAGE AND STABILITY

    Frozen DVS® cultures should be stored at -45°C(-49°F) or below. If frozen DVS® cultures are

    stored correctly, the shelf life is at least 12

    months.

    Freeze-dried DVS® cultures should be stored

    at -18°C (0°F) or below. If freeze-dried

    cultures are stored correctly, the shelf life is

    at least 24 months. At +5°C (41°F) the shelf

    life is at least 6 weeks.

    PACKAGING 

    Frozen DVS® cultures, as a standard, come in

    10 x 500 unit disposable plastic bags co-packed in a cardboard box. For shipping, the

    boxes are packed in a special plastic foam

    box with dry ice. For certain products, other

    packaging sizes than 10 x 500 units are

    available.

    Freeze-dried DVS® cultures are supplied in

    alufoil pouches and are available in the

    following standard sizes:

    50 unit pouch - 10 pouches per box

    200 unit pouch - 25 pouches per box

    500 unit pouch - 20 pouches per box

    SHIPMENT TERMS

    For frozen DVS® cultures, the total shipment

    time from Chr. Hansen to the customer

    should not exceed 72 hours. Freeze-dried

    DVS® cultures may be transported at room

    temperature for up to ten days, without the

    quality of the cultures being affected.

    APPLICATION

    DVS® for regular cheese milk

    The cheese milk is inoculated with the

    culture in the cheese vat or tank. The cheese

    milk may require 20-40 minutes of pre-

    ripening before applying rennet. However,

    normal cheese-making procedures and time

    limits can be obtained by adding appropriate

    amounts of CaCl2 to the cheese milk.

    DVS® for UF concentrated milk

    Frozen DVS® cultures allow fermentation of

    UF concentrated milk, without dilution of

    the concentrate.

    DOSAGE

    Recommended standard inoculation rates

    are given below. However, specific usage

    rates should be determined experimentally

    prior to any new application.

    Application Frozen

    DVS®

    Freeze-dried

    DVS®

    Cheese 50-150 units

    per 1000 L

    50-150 units

    per 1000 L

    GUARANTEE

    Chr. Hansen's dairy cultures are produced in

    conformity, with the regulations of the local

    health authorities.

    TECHNICAL SERVICE

    Chr. Hansen has internationally dispersed

    development centers, as well as application

    centers in all major dairy markets. For

    specific application advice and support,

    please contact your local Chr. Hansen

    representative or visit us at www. chr-

    hansen.com.

    CULTURE PERFORMANCE IN THIS

    CATALOGUE

    All the cheese culture series, as well asindividual cultures, are rated on a scale from

    1 - 5 for Acidification speed , Phage

    robustness, Contribution to flavor , and

    when relevant also for Gas production. 1 is

    low and 5 is high. The rating of a culture

    series is based on the average performance

    of the individual cultures in the series. This

    means that the score of the individual

    cultures will sometimes vary slightly from

    the rating of the series. Some culture series

    in this catalogue are described under several

    different cheese segments. The rating can be

    different for the same series since the

    typical culture application may vary fromsegment to segment.

    Example of how the DCC series from the

    continental segment is rated:

    Low High

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    PACKAGING 

    Frozen DVS® cultures, as a standard, come in

    10 x 500 unit disposable plastic bags co-

    packed in a cardboard box. For shipping, the

    boxes are packed in a special plastic foambox with dry ice. For certain products, other

    packaging sizes than 10 x 500 units are

    available.

    Freeze-dried DVS® cultures are supplied in

    alufoil pouches and are available in the

    following standard sizes:

    50 unit pouch - 10 pouches per box

    200 unit pouch - 25 pouches per box

    500 unit pouch - 20 pouches per box

    SHIPMENT TERMS

    For frozen DVS® cultures, the total shipmenttime from Chr. Hansen to the customer

    should not exceed 72 hours. Freeze-dried

    DVS® cultures may be transported at room

    temperature for up to ten days, without the

    quality of the cultures being affected.

    APPLICATION

    DVS® for regular cheese milk

    The cheese milk is inoculated with the

    culture in the cheese vat or tank. The cheese

    milk may require 20-40 minutes of pre-

    ripening before applying rennet. However,

    normal cheese-making procedures and time

    limits can be obtained by adding appropriate

    amounts of CaCl2 to the cheese milk.

    DVS® for UF concentrated milk

    Frozen DVS® cultures allow fermentation of

    UF concentrated milk, without dilution of

    the concentrate.

    DOSAGE

    Recommended standard inoculation rates

    are given below. However, specific usage

    rates should be determined experimentally

    prior to any new application.

    Application Frozen

    DVS®

    Freeze-dried

    DVS®

    Cheese 50-150 units

    per 1000 L

    50-150 units

    per 1000 L

    GUARANTEE

    Chr. Hansen's dairy cultures are produced in

    conformity, with the regulations of the local

    health authorities.

    TECHNICAL SERVICE

    Chr. Hansen has internationally dispersed

    development centers, as well as application

    centers in all major dairy markets. For

    specific application advice and support,

    please contact your local Chr. Hansen

    representative or visit us at www. chr-

    hansen.com.

    CULTURE PERFORMANCE IN THIS

    CATALOGUE

    All the cheese culture series, as well as

    individual cultures, are rated on a scale from1 - 5 for Acidification speed , Phage

    robustness, Contribution to flavor , and

    when relevant also for Gas production. 1 is

    low and 5 is high. The rating of a culture

    series is based on the average performance

    of the individual cultures in the series. This

    means that the score of the individual

    cultures will sometimes vary slightly from

    the rating of the series. Some culture series

    in this catalogue are described under several

    different cheese segments. The rating can be

    different for the same series since the

    typical culture application may vary from

    segment to segment.

    Example of how the DCC series from the

    continental segment is rated:

    Low High

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

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    11

     Cheddar Cheese Cultures

    Introduction to Cheddar Cheese Cultures

    The figure below provides an overview of the different culture series that belong to the Cheddar cheese “family”. The cultures vary

    significantly from each other as far as flavor contribution to the cheese and acidification speed. Generally, the cultures that are located in the

    far right of the figure will have high flavor contribution due to the presence of Lactobacillus strains.

     A  c  i   d  i   f   i   c  a t   i   o n S  p e e d  

    Fast DVS® RST DVS® RSF

    Medium DVS® R

    Slow

    Culture Composition

     A  c  i   d  i   f   i   c  a t   i   o n S 

     p e e d  

    Fast DVS® RST DVS® RSF

    Medium DVS® R

    Slow

    Traditional type

    cheddar flavor

    Irish type cheddar

    “Sweet and nutty” 

    Flavor Profile 

    O O 

    ST 

    ST 

    LbH 

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     Cheddar Cheese Cultures

    DVS® R

    One of the major reasons behind the success of Chr. Hansen is the R series solutions. The R series includes a full range of cultures for various

    cheese productions to fit the demands of the modern cheese producer. This mesophilic homofermentative culture range contains O strains

    known particularly for fast acidification and phage resistance which is acknowledge by cheese makers through many years and geographies.

    Within the R series, 03 and 04 are the most phage robust, whereas 07 and 08 are known for good savory flavor formation. Lastly, it is

    recommended to use this series in a culture rotation system.

    Culture Composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form

    This series is available in both frozen and freeze-dried form in all standard packaging sizes. 

    DVS® RST

    This series is a traditional homofermentative blend of mesophilic O culture strains and a thermophilic strain. The cultures in this series are

    known for their excellent phage robustness and fast acidification speed. This culture works best when the cheese process includes scalding

    temperatures at 37°C (99°F) or higher, which then typically will result in a faster cheese make time and also a more phage robust performance

    compared to all mesophilic blends. Additionally, it is recommended to use this series in a culture rotation system.

    Culture Composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor: 

    Product Form

    This series is available in both frozen and freeze-dried form in various packaging sizes.

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

    ST O 

    Storage: -45°C/-49°F

    Culture composition

    Culture composition

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     Cheddar Cheese Cultures

    DVS® RSF

    This series is based on homofermentative cultures consisting of mesophilic O culture strains, a thermophilic strain and a Lactobacillus

    helveticus strain. The cultures in this series are known for their excellent phage robustness and fast acidification speed. Due to the presence of

    the Lactobacillus helveticus  strain, the culture will add sweet and nutty flavor notes to the ripened cheese. For a more intense note of the

    sweet and nutty flavors, the RSF-621 culture is recommended due to the higher ratio of Lactobacillus helveticus strains than the other cultures

    in this series. Moreover, the RSF cultures work best when the cheese process includes scalding temperatures at 37°C (99°F) or higher, which

    then typically will result in a faster cheese make time and also a more phage robust performance compared to all mesophilic blends. Also it is

    recommended to use this series in a culture rotation system.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form

    This series is available in both frozen and freeze-dried form in various packaging sizes.

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

    ST 

    LbH 

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     Continental Cheese Cultures

    Introduction to Continental Cheese Cultures

    The continental cheeses are typified by semi-hard yellow cheeses as the Gouda and Edam types traditionally made in the Netherlands, but

    now produced worldwide for considerable international trade. Most important parameters are overall consistency of the finished cheese

    (acidification, flavor and texture), more control for the cheese maker and convenient to use. Culture types covers traditional DVS® LD cultures,

    but also blended cultures like DVS® DCC-type and the latest innovation of EASY-SET® FLORA™ C-series. A wide range of SWING® cultures for

    smear coat (Brevibacterium linens) and DVS® adjunct cultures for ripening or propionic cheeses can be used as a supplement and innovative

    cheeses or efficient ripening. The figure below provides an overview of the outstanding range of DVS® and EASY-SET® FLORA ™ culture series

    that belong to the Continental cheese “family”. 

     A  c  i   d  i   f   i   c  a t   i   o n

     S  p e e d  

    Fast DVS® DCC

    Medium DVS® R serieEASY-SET®

    FLORA™ C100 

    EASY-SET®

    FLORA™ C900

    EASY-SET®

    FLORA™ C1000

    SlowDVS® CH-N/

    Flora Danica

    EASY-SET®

    FLORA™ C500

    Culture Composition 

     F  l   a v o r  i   n t   e n s  i   t   y

     

    High

    DVS® Flora Danica, CH-N,

    DCC. EASY-SET® FLORA™ 

    C500 & C1000

    Medium EASY-SET® FLORA™ C100 EASY-SET® FLORA™ C900

    Low DVS® R serie

    No eyes

    ”Blind cheese”  Few (Pin holes) Few-Medium

    Eye Formation 

    LD  LD O  O  LD  O  O 

    ST  L L 

    D LbH 

    LbP 

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     Continental Cheese Cultures

    DVS® Flora Danica/CH-N

    These series consist of several different traditional LD cultures that are all multiple defined blends of mesophilic strains. The cultures are

    citrate fermenting heterofermentative and will form eyes of various sizes in the finished cheese. The many different strains in the blend

    ensure extra good protection against bacteriophage. This series - and especially Flora Danica - is known for its exceptional “full flavor” that it

    provides to the finished continental cheese, whereas CH-N-19 is known for a very phage robust performance. Lastly, this culture series has

    optimum performance at scalding temperatures between 35-38°C (95-100 °F).

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in various frozen and freeze-dried packaging sizes.

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

    LD 

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     Continental Cheese Cultures

    DVS® DCC

    This series consists of defined mixes of several different mesophilic and termophilus strains.

    The DCC cultures are blends of LD, O, ST, LbH and LbP cultures. These blended cultures will increase the phage protection, enhance the

    rounded flavor and reduce the bitterness in the final cheese. The range is mainly used for scalding temperatures at 32-42°C.

    Culture composition

    Culture Performance 

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in various frozen and freeze-dried packaging sizes.

    DVS® R

    This series is a traditional homofermentative culture consisting of 100% mesophilic O culture strains, known particularly for their phage-

    resistant properties. Within this series, R-03 and R-04 are the most phage robust, whereas R-07 and R-08 are known for good flavor formation.

    It is recommended to use this series in a culture rotation system.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in both frozen and freeze-dried form in all standard packaging sizes.

    Storage: -45°C/-49°F

    Frozen

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

    LD  ST

     

    LbH 

    LbP  O

     

    Storage: -18°C/0°F

    Freeze-dried

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     Continental Cheese Cultures

    EASY-SET® FLORA™ C100

    This EASY-SET® FLORA™ C100 series is a blend of O cultures known for its high activity, suitableness at scalding temperatures up to 38°C (100°F)

    and good flavor contribution. The culture solution is very phage robust and of carefully selected mesophilic O culture strains. The culture is

    homofermentative and will not contribute to eye formation in the finished cheese, but it provides mild to medium mature flavor profile. EASY-

    SET® FLORA is a very phage robust series where only limited culture rotation is needed flowing the attitude “no rotation, but vacation/break”.  

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in frozen form.

    EASY-SET® FLORA™ C500

    This EASY-SET® FLORA™ C500 series is a blend of traditional LD cultures known for providing an intense full flavor to the finished cheese.

    Within the continental range of cultures, this is considered a “slow to medium” speed culture, designed for cheese processes with five to six

    hours from culture addition to brine. Only pure mesophilic cultures are used in the C500 series and typical cheeses are Gouda, Edam,

    Maasdammer/ Leerdammer, St Paulin, Cantal, Tomme, Raclette, and local Swedish cheese types such as: Herrgårdsost, Grevéost, Prästost,

    Hushållost and local Danish types such as: Samsø, Havarti and Danbo.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in frozen form.

    Storage: -45°C/-49°F

    Frozen

    Storage: -45°C/-49°F

    Frozen

    LD 

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     Continental Cheese Cultures

    EASY-SET® FLORA™ C900

    This EASY-SET® FLORA™ series is a primary choice for fast cultures to main continental market. This EASY-SET® series is a blend of carefully

    selected O and L cultures known for fast acidification and production of cheese where only a limited eye fo rmation is required. The FLORA™

    C900 is designed for cheese processes with four to six hours from culture addition to brine and cheese with mild flavor profile. Within the

    continental range of cultures, the FLORA C900 series is designed for Edam, Gouda, Tilsiter, Butterkäse, Manchego or Raclette types of cheeses

    with relative mild flavor profile and few eyes. Additionally, the FLORA™ C900 is also attractive for Maasdammr/Leerdammen, Wilstermarch

    (Germany), St Paulin, Cantal, Tomme, Golandskiy, Kostromskoy and Rossiyskiy (Russia and Eastern Europe). EASY-SET® FLORA™ is a very phage

    robust series where only limited culture rotation is needed flowing the attitude “no rotation, but vacation/break”.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in frozen form.

    EASY-SET® FLORA™ C1000

    This EASY-SET® FLORA™ C1000 series is a blend of carefully selected L, D and O cultures known for providing an intense, full flavor, many eyes

    and smooth texture to the finished cheese. Within the continental range of cultures, this is considered a “slow to medium” speed culture,

    designed for cheese processes with five to six hours from culture addition to brine. Only pure mesophilic cultures are used in the EASY-SET ® 

    FLORA™ C1000 series and typical cheeses are Gouda, Edam and Maasdammer. EASY-SET® FLORA™ is a very phage robust series where only

    limited culture rotation is needed flowing the attitude “no rotation, but vacation/break”.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in frozen form.

    Storage: -45°C/-49°F

    Storage: -45°C/-49°F

    Frozen

    D

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     Cottage Cheese Cultures

    Introduction to Cottage Cheese Cultures

    The figure below provides an overview of the different culture series that belong to the Cottage cheese ‘”family”. The cultures mainly differ

    from each other in cheese yield, acidification speed and phage robustness. Fresco ®  cultures are especially designed to meet strong

    performance requirement of cultures in the cottage cheese industry. These cultures contain special strains of Lactococcus lactis subsp. lactis,

    of Lactococcus lactis subsp. cremoris, and Streptococcus thermophilus, selected for their phage robustness, fast acid production, and

    particularly for Fresco® 3000 with excellent yield.

     A  c  i   d  i   f   i   c  a t   i   o n

     S  p e e d  

    Fast Fresco®

    Medium DVS® CCDVS® R

    Slow

    Culture Composition

     A  c  i   d  i   f   i   c  a t   i   o n S  p e e d  

    Fast Fresco® 1000 Fresco® 3000

    Medium DVS® CCDVS® R

    Slow

    Medium High

    Cheese Yield  

    O  O 

    ST 

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     Cottage Cheese Cultures

    Fresco® 1000

    Fresco® 1000 is a blend of mesophilic O culture strains and a thermophilic strain that is designed to meet requirements in the cottage cheese

    industry. Moreover, this series is known for very high phage robustness and fast acidification speed. The culture adds a nice clean fresh flavor

    to the cottage cheese with no bitterness. Lastly, it is recommended to use this series in a culture rotation system.

    Culture Composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form

    This series is available in frozen form in 1000 U packaging sizes.

    Fresco® 3000

    This series is a blend of mesophilic O culture strains and a thermophilic strain that is designed to meet requirements in the cottage cheese

    industry. The series offers all the same advantages as the Fresco® 1000 like: Consistent set times and production procedures, increased plant

    throughput, and improved phage robustness. Innovation has taken Fresco® 3000 to the next level and now it also offers: A sophisticated way

    to increase cheese yield, and an improved curd identity and texture. Due to excellent water binding properties of some of the strains in the

    Fresco® 3000 range, a cottage cheese plant will typically see an improvement in cheese yield of 2-5%. This additional curd yield comes mostly

    from water that is bound in a sophisticated matrix, which ensures improvement of the curd quality and mouth feel. Lastly, it is recommended

    to use this series in a culture rotation system.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:Contribution to flavor:

    Product Form

    This series is available in frozen form in 1000 U packaging sizes.

    Storage: -45°C/-49°F

    Frozen

    Storage: -45°C/-49°F

    Frozen

    ST 

    ST 

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     Cottage Cheese Cultures

    DVS® CC

    This series is a traditional homofermentative culture consisting of 100% mesophilic O culture strains, known particularly for their phage

    resistant properties. This series produces whey with very little residual galactose, providing excellent drying properties of the whey.

    Additionally, it is recommended to use this series in a culture rotation system. 

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form

    This series is available in frozen form in standard 500 U packaging sizes.

    DVS® R

    One of the major reasons behind the success of Chr. Hansen is the R series solutions. The R series includes a full range of cultures for various

    cheese productions to fit the demands of the modern cheese producer. This mesophilic homofermentative culture range contains O strains

    known particularly for rapid acidification and phage resistance which is acknowledge by cheese makers through many years and geographies.

    Within the series, 03 and 04 are the most phage robust, whereas 07 and 08 are known for good savory flavor formation. Lastly, it is

    recommended to use this series in a culture rotation system.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form

    This series is available in both frozen and freeze-dried form in all standard packaging sizes.

    Storage: -45°C/-49°F

    Frozen

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

    Culture composition

    Culture composition

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     Pasta Filata Cheese Cultures

    Introduction to Pasta Filata Cheese Cultures

    The figure below provides an overview of the many different culture series that belong to the Pasta Filata cheese “family”. The cultures mainly

    differ from each other in acidification speed, flavor profile and phage robustness.

     A  c  i   d  i   f   i   c  a t   i   o n S  p e e d 

    Fast DVS® STI

    Medium DVS® TCC DVS® TCC-20

    Slow

    Culture Composition 

     M o i   s  t   u r  e r  e t   e n t   i   o n

     (   C  a p s  u l   a r  p o l   y s  a c  c  h  a r  i   d  e p r  o d  u c 

     t   i   o n )  

     

    High

    Medium DVS® STI  DVS® TCC DVS® TCC-20

    Low

    Traditional fresh lactic

    acid Mozzarella/Pasta Filata flavor

    “Yoghurt-like flavor”,

    Mediterranean style

    “Sweet and nutty”

    flavor notes and verylimited browning

    during baking

    Flavor profile / functionality  

    ST  ST  ST 

    LbH LbB 

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     Pasta Filata Cheese Cultures

    DVS® STI

    This series is a complex blend of multiple-defined thermophilic strains that have been designed to provide the cheese plant with unique

    protection against bacteriophages, while achieving a very consistant and fast acidification. DVS® STI builds on state-of-the-art compounding

    technology and is known for mild flavour notes and a very fast acidification speed. Lastly, it is recommended to use this series in a culture

    rotation system.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form

    This series is available in both frozen and freeze-dried form in various packaging sizes.

    DVS® TCC

    This series is a blend of thermophilic strains and Lactobacillus bulgaricus, providing the finished cheese with a traditional Mediterranean type

    of Pasta filata flavor. This culture should be used when yoghurt-like notes are desired in the finished Pasta filata cheese, and generally if

    increased flavor intensity is desired. Additionally, it is recommended to use this series in a culture rotation system.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form

    This series is available in freeze-dried form in some standard packaging sizes.

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Storage: -18°C/0°F

    Freeze-dried

    ST 

    ST  LbB 

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

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     Pasta Filata Cheese Cultures

    DVS® TCC-20

    TCC-20 is not a series, but a unique product which consists of a blend of Streptococcus thermophilus and Lactobacillus helveticus strains. The

    galactose level in the finished cheese is quite low for this particular culture, which makes the culture ideal for producing Pasta filata types of

    cheese with only limited browning during baking or heating.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form

    This product is available in both frozen and freeze-dried form in various packaging sizes. 

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

    ST 

    LbH 

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     Soft Cheese Cultures

    Introduction to Soft Cheese Cultures

    The figure below provides an overview of the different culture series that belong to the Soft cheese “family”. The cultures mainly differ from

    each other in acidification speed, flavor intensity, eye formation and water retention in the cheese.

     A  c  i   d  i   f   i   c  a t   i   o n S  p e e d  

    Fast DVS® BA

    Medium DVS® CZ/ST-B/SSC DVS® YF

    Slow DVS® CH-N DVS® SDMB DVS® Mild O DVS® STELLA

    Culture Composition 

     F  l   a v o r  I   n t   e n s  i   t   y

    High DVS® STELLA DVS® Flora/CH-N

    Medium DVS® Mild O DVS® SDMB

    LowDVS® ST-

    B/SSC/CZ/BA/YF

    No eyes

    ”Blind cheese”  Few (Pin holes) Few-Medium

    Eye Formation 

    LD  D  ST  O 

    LbB 

    ST 

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     Soft Cheese Cultures

    DVS® Flora/CH-N

    Flora and CH-N are unique set of solutions build on extensive field experience and designed by combining many mesophilic strains. This mak es

    you able to fully capitalize on the value of consistency in cheese production. The cultures are heterofermentative and will form eyes of various

    sizes in the finished cheese. The many different strains in the cultures ensure good protection against bacteriophage. These series - and

    especially Flora Tradi and Flora Fresh – are known for the exceptional “full flavor” that they provide to the finished Soft cheese. Additionally,

    these culture series has optimum performance at scalding temperatures below 37°C (99°F).

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in various frozen and freeze-dried packaging sizes.

    DVS® Mild O

    This series is a traditional homofermentative culture consisting of 100% mesophilic complex O culture strains. The culture is based on low

    proteolytic strains, resulting in very mild flavor notes with no bitterness in the finished cheese. It allows for a cheesy mesophilic flavor without

    getting holes or floating curds in the cheese vat. This culture is widely used for lactic curds like goat cheeses, and it can be used in blends with

    CH-N, D or STI series.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form

    This series is available in 500g frozen cartons and in a freeze-dried form.

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

    LD 

    Storage: -45°C/-49°F

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     Soft Cheese Cultures

    DVS® SDMB

    This series consists of several different cultures that are defined blends of Lactococcus lactis spp. lactis biovar diacetylactis. The cultures are

    homofermentative, but can form eyes in the finished cheese by fermentation of citrate. Moreover, the D cultures are known for flavoring

    properties, bringing fresh and creamy notes to the final cheese. The cultures have optimum performance at scalding temperatures between

    35-38°C (95-100°F). Additionally, the cultures are usually blended together with LD, O or ST series and are seldom used alone.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in 500g frozen cartons.

    DVS® ST-B / SSC

    These series are based on thermophilic strains with a mild acidification. SSC-100, -102 and -103 are new robust cultures avoiding post-

    acidification. They bring clean taste and smooth mouth feeling. SSC-17 is a specific culture that stabilizes at a high pH value (about pH 5.2). SSC

    and STB can be used alone or in combination with CHN/Flora, R series or SDMB cultures according to the characteristics of the final cheese.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in various frozen and freeze-dried packaging sizes.

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

    ST 

    Storage: -45°C/-49°F

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     Soft Cheese Cultures

    DVS® CZ

    This series is based on thermophilic strains that initiate acidification fast and stabilize pH during storage. Moreover, the cultures are able to

    generate polysaccharides that retain water, which gives a smooth mouth feeling. Additionally, the cultures can be rotated and the main

    application is Italian Crescenza.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in various frozen 500g cartons.

    DVS® BA

    This series is based on thermophilic strains and associate very fast acidification speed with high texturing properties. The cultures are able to

    generate polysaccharides that retain water, which gives a smooth mouth feeling. The main application is Argentinean Port Salut type of cheese.

    It is recommended to use this series in a culture rotation system.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in frozen form of 250 U pack sizes.

    Storage: -45°C/-49°F

    Frozen

    ST 

    ST 

    Storage: -45°C/-49°F

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     Soft Cheese Cultures

    DVS® YF

    These series consist of blends of ST and Lactobacillus bulgaricus strains devoted to cheese acidification. The main applications are blue molded

    cheeses with specific requirements to the cheese body, e.g. a very smooth texture. Both series are known for a very “rounded“ flavor profile.

    Additionally, these cultures can be applied in Gorgonzola and Vacherin types of cheeses.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in frozen form.

    DVS® STELLA

    This unique culture of Lactobacillus bulgaricus enables a deep acidification during drainage and a high contribution in ripening due to its rich

    enzyme content. It is devoted to blue cheeses, e.g. Gorgonzola types, and Soft cheeses with very smooth texture in mouth and no bitterness.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in frozen form.

    Storage: -45°C/-49°F

    Frozen

    ST 

    LbB 

    LbB  ST 

    Storage: -45°C/-49°F

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     Propionic Cheese Cultures

    Introduction to Propionic Cheese Cultures

    The figure below provides an overview of the different culture series that belong to the Propionic cheese “family”. The cultures mainly differ

    from each other in acidification speed, eye formation and flavor intensity.

     A  c  i   d  i   f   i   c  a t   i   o n S  p e e d  

    Fast DVS® STEM

    Medium DVS® DCC DVS® LH

    Slow DVS® CH-N/Flora Danica

    None DVS® PS

    Culture Composition 

     F  l   a

     v o r  I   n t   e n s  i   t   y

    High DVS® LHDVS® DCC/CH-N/

    Flora DanicaDVS® PS

    Medium

    Low DVS® STEM

    No eyes

    ”Blind cheese”  Few (Pin holes) Many (very big)

    Eye Formation 

    LD  LD 

    ST 

    ST 

    LbH 

    LbH 

    LbP 

    PAB 

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     Propionic Cheese Cultures

    DVS® Flora Danica/CH-N

    Flora Danica and CH-N are unique set of solutions build on extensive field experience and designed by combining many mesophilic strains. This

    makes you able to fully capitalize on the value of consistency in cheese production. The cultures are heterofermentative and will generate pin

    holes depending on the scalding temperatures. The many different strains in the blend ensure good protection against bacteriophage. These

    series - and especially the Flora Danica and CH-N-19 - are known for the exceptional “full flavor” that they provide to the finished cheese.

    These culture series has optimum performance at scalding temperatures below 37°C (99°F), so the use for acidification is restricted to

    Maasdam type of cheese.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form 

    This series is available in various frozen and freeze-dried packaging sizes.

    DVS® DCC

    This series consists of several different cultures that are all multiple defined blends of many different mesophilic strains. The cultures are

    heterofermentative and will generate pin holes depending on the scalding temperatures. This group of cultures is recommended for Propionic

    cheeses, since they mainly consist of LD culture and carefully selected O culture strains added with ST, LbH, and LbP. The cultures are intended

    for production procedures with scalding temperatures between 37-42°C (99-108°F). The many different strains in the blend ensure good

    protection against bacteriophage.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in various frozen and in a few freeze-dried packaging sizes.

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

    LD 

    LD  ST  LbH  LbP  O 

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

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     Propionic Cheese Cultures

    DVS® STEM

    This series is based on thermophilic strains resistant to high scalding temperatures. The series consists of different ST cultures for rotation

    devoted to Emmenthal. Also, STEM-02 allows the cheese curd to retain more water. It is recommended to use this series in a culture rotation

    system.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in frozen form.

    DVS® LH

    This series is based on Latobacillus helveticus. The cultures vary in intensity of proteolysis, extent of acidification, flavor as well as their

    resistance to scalding temperatures. This allows for stimulation of the growth of propionic acid cultures. Due to their natural resistance to

    bacteriophage, the cultures do not need any rotation.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in various frozen and freeze-dried packaging sizes.

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

    ST 

    LbH 

    Storage: -45°C/-49°F

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     Propionic Cheese Cultures

    DVS® PS

    This series is a single strain species of Propionibacterium freudenreichii subsp freudenreichii  or subsp shermanii . The cultures are not able to

    acidify, but will ferment lactate and produce propionate, acetate and carbon dioxide. Additionally, the cultures will provide the finished

    cheese with characteristic sweet notes and famous large holes.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in various frozen and freeze-dried packaging sizes.

    Difference in Flavor Intensity and Eye Formation

     F  l   a v o r  I   n t   e n s  i   t   y

    Typical Rich

    Propionic DVS® PS-40 

    Medium/Rich DVS® PS-2  DVS® PS-60

    Mild/Medium

    Mild DVS® PS-1 DVS® PS-80

    Few Few-Medium Medium-Rich Rich/Multiple

    Eye Formation 

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

    PAB 

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     Grana Cheese Cultures

    Introduction to Grana Cheese Cultures

    The figure below provides an overview of the different culture series that belong to the Grana cheese “family”. The cultures mainly differ from

    each other in functionality and flavor intensity.

     A  c  i   d  i   f   i   c  a t   i   o n S  p e e d  

    Fast

    Medium

    Slow DVS® Grana DVS® MLC-300 DVS® TCC-20 

    Culture Composition 

     F  l   a v o r  I   n t   e n s  i   t   y

    High DVS® Grana

    Medium DVS® TCC-20 DVS® MLC-300

    Low

    Traditional Grana “hard

    cheese” taste and

    maturation

    Enhances the white color of

    the cheese due to high

    galactose consumption

    Functionality

    LbH 

    ST 

    LbB 

    LbP 

    ST 

    LbP 

    ST 

    LbH 

    LbL 

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     Grana Cheese Cultures

    DVS® Grana

    This series is composed of thermophilic strains in combination with a blend of Lactobacillus helveticus, Lactobacillus bulgaricus  and

    Lactobacillus paracasei . This culture series is used for long time ripening types of cheeses like Grana and Parmesan, where especially the

    Lactobacillus helveticus contributes to the special flavor development during the ripening. Lastly, the complexity of the Grana culture blends

    make them very robust against bateriophages.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form 

    This series is available in a standard frozen package size.

    DVS® MLC-300

    This series is suitable for Pecorino or similar types of cheese, where a white color of the cheese is desired. The culture contains Lactobacillus

    debruecki ssp lactis strains, which ensures high galactose consumption without development of the typical "hard cheese" taste, caused by

    Lactobacillus helveticus in other Grana cultures.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form

    This series is available in freeze-dried form. 

    Storage: -18°C/0°F

    Freeze-dried

    LbH  ST  LbB  LbP 

    LbP LbL ST 

    Storage: -45°C/-49°F

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     Grana Cheese Cultures

    DVS® TCC-20

    TCC-20 is a unique product which consists of a blend of Streptococcus thermophilus and Lactobacillus helveticus strains. The culture can be

    used in production of Grana and Parmasan types of cheeses, where it gives flavor notes that are characteristic for the hard and low moisture

    cheese types.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form

    This product is available in all standard packaging sizes.

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

    ST 

    LbH 

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     White Brined Cheese Cultures

    Introduction to White Brined Cheese Cultures

    The figure below provides an overview of the different culture series that belong to the W hite brined cheese “family”. This culture group

    covers traditional Feta and similar White cheeses like Halloumi, Domiati, Telemea and Bjalo. The cultures mainly differ from each other in

    acidification speed and flavor intensity.

     A  c  i   d  i   f   i   c  a t   i   o n S  p e e d  

    Fast DVS® FRC DVS® R

    Medium DVS® Mild O DVS® SafeIT 

    Slow

    Culture Composition 

     F  l   a v o r  I   n t   e n s  i   t   y

    High

    Medium DVS® FRC/R DVS® SafeIT 

    Low DVS® Mild O

    Traditional processUltra filtration

     process

    Cheese process/technology

    O  O  O 

    ST  ST 

    LbB  LbB 

    LbH 

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     White Brined Cheese Cultures

    DVS® FRC 

    FRC is a traditional homofermentative blend of mesophilic O culture, thermophilic Streptococcus thermophilus and Lactobacillus bulgaricus 

    strains. The cultures are known for good phage robustness, fast acidification speed and closed cheese texture, especially preferred in

    traditional white cheese made in the Balkan region. It is recommended to use this series in a culture rotation system. The recommended

    production temperature is 35°C (95°F).

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form

    This series is available in a freeze-dried form.

    DVS® R

    One of the major reasons behind the success of Chr. Hansen is the R series solutions. The R series includes a full range of cultures for various

    cheese productions to fit the demands of the modern cheese producer. This mesophilic homofermentative culture range contains O strains

    known particularly for rapid acidification and phage resistance which is acknowledge by cheese makers through many years and geographies.

    Within the series, 03 and 04 are the most phage robust, whereas 07 and 08 are known for good savory flavor formation. Lastly, it is

    recommended to use this series in a culture rotation system.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form

    This series is available in both frozen and freeze-dried form in all standard packaging sizes. 

    Storage: -18°C/0°F

    Freeze-dried

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-ried

    ST LbBO 

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     White Brined Cheese Cultures

    DVS® Mild O

    Part of white brined cheese production is made with 100% mesophilic O culture. The Mild O is 100% mesophilic culture strains, known

    particularly for their phage resistant properties. The Mild O is unique in sense no post-acidification and used when a gentle acidification is

    preferred to avoid bitterness.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form

    This series is available in both frozen and freeze-dried form in various packaging sizes.

    DVS® SafeIT

    SafeIT is developed for the industrial UF white brined cheeses from the Middle East and Balkan regions. It is a blend of both mesophilic and

    thermophilic strains. SafeIT is designed to improve texture, flavor and bring more certainty to the production, in terms of speed of

    fermentation and bactoriophage robustness. The recommended composition of the retentate is: Fat: 15-17,5%; Protein: 14-15%; TS:35-40%

    and optimum fermentation temperatures at 34-35°C.

    Culture composition

    Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Product Form

    This series is available in a freeze-dried form.

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Storage: -18°C/0°F

    Freeze-dried

    O  ST  LbH  LbB 

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

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     Ripening Cultures

    Overview of Ripening Cultures

    The cultures in the ripening range vary significantly with different species and many different applications. The figure below provides an

    overview of the different culture series that belong to the ripening “family”. The blue colored products are mainly used for surface ripened

    soft cheese types, and for some smeared continental type of cheeses. The black  colored products are mainly used for special flavor

    developments in propionic, continental and cheddar type cheese.

     C  u l   t   u r  e C  o m p o s  i   t   i   o n

     

    PC

    Penicillium candidum

    PR

    Penicillium roqueforti

    GEOGeotrichum

    candidum

    Yeast

    Debaryomyces

    kluyveromyces

    BL/BC

    Brevibacterium

    SALSA

    Staphylococcus

     xylosus

    PS

    Propionic bacteria

    CR-200-300

    Lactococcus

    CR-500/Full Flavor

    Lactococcus and

    Lactobacillus

    and Pediococcus

    LH and EMFOUR

    Lactobacillus

    helveticus

    Mold Yeast Bacteria

    Type of Microorganism 

    LbH  LbP 

    Lb rh  Lb cu  Lb pl  Lb jo  P ac 

    LbH 

    PAB 

    PC 

    PR 

    GEO 

    LAF 

    BL 

    SALSA 

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     Ripening Cultures

    SWING®: Ripening cultures for soft mold types of cheeses and smeared cheeses 

    The cultures below are aerobic ripening cultures mainly recommended for soft cheeses; Camembert and Brie types as well as blue cheeses,

    mixed rinds (with red and white surface) and smeared cheeses. The cultures originate from traditional cheeses or brines, and they have been

    selected for their appearance and/or flavoring properties.

    Flavor Development  

    D e g r  e e o f  L  i   p o l   y s  i   s 

    High PC Penicillium candidumPR Penicillium roqueforti

    Medium GEOGeotrichum Candidum

    SALSA

    Staphylococcus xylosus

    Low LAF Yeast  BL Brevibacterium BC Brevibacterium

    Low Medium High

    Degree of Proteolysis 

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     Ripening Cultures

    SWING® PC - Penicillium candidum

    This series is composed of Penicillium candidum selected for their bright white appearance and moderate metabolic activity. The cultures

    allow for a white and short homogenous rind. Moreover, PC-A1 is well known for contributing to a longer shelf life, which is due to the low

    proteolytic activity. Lastly, PC-A3 ensures protection against mucors.

    Culture composition Culture Performance

    Growth speed:

    Contribution to coating:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in freeze-dried spores with packaging sizes of 10 and 50 units.

    SWING® PR/PRG - Penicillium roquefortiThe PR series is composed with two sub-ranges of Penicillium roqueforti . The PR series is recommended for traditional blue cheeses, whereas

    the PRG cultures were selected for the mild color they bring to Gorgonzola. The cultures in both series differ in color as well as in proteolytic

    and lipolytic activity. Lastly, PR-1 is well known for its low proteolytic activity, and is recommended for small size cheeses and double cheeses

    (blue on the inside and white on the outside).

    Culture composition Culture Performance

    Growth speed:

    Contribution to coating:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in freeze-dried spores with packaging sizes of 10 units.

    Storage: -18°C/0°F

    Storage: -18°C/0°F

    Freeze-dried

    PC 

    PR

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     Ripening Cultures

    SWING® GEO - Geotrichum candidum

    This series is composed of strains from yeast. The cultures contribute to the cheese coating, forming a thin rind and bringing a typical flavor to

    the cheese, while decreasing bitterness. Lastly, some strains - e.g. GEO CB - are able to inhibit the growth of contaminant molds.

    Culture composition Culture Performance

    Growth speed:

    Coating quality:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in liquid arthrospores form in 10 units bottles. The product should be stored in a cool place at 2-4°C (36-39°F).

    SWING® LAF - YeastThis series consists of strains from various species selected for targeted purposes  – both neutralizing and fermenting yeast. Neutralizing yeast

    is devoted to the consumption of lactate resulting in rise of pH, and they develop on the cheese rind. Fermenting yeast produces flavor in the

    cheese curd, and they grow preferably inside the cheese. The SWING® TRIO is a blend of carefully appointed yeasts, allowing bioprotection

    towards unwanted molds on the surface of soft cheese and continental cheese types.

    Culture composition Culture Performance

    Growth speed:

    Contribution to coating:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in freeze-dried packaging sizes of 10 units.

    Storage: -18°C/0°F

    Freeze-dried

    GEO

    LAF

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     Ripening Cultures

    SWING® BL/BC - Brevibacterium

    The BL series consists of Brevibacterium linens, whereas the BC series is a strain of Brevibacterium casei . BL gives an orange color to the cheese

    rind with a typical sulphur flavor. BC provides a high aminopeptidase activity and plays a significant role in the cheese texture, resulting in a

    round mouth feeling, softness and reduction of bitterness. 

    Culture composition Culture Performance

    Growth speed:

    Contribution to coating:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in freeze-dried packaging sizes of 10 units.

    SWING® SALSA - Staphylococcus xylosus

    The SALSA series is devoted to cheese flavoring. Especially SALSA-1 provides a typical aroma of raw milk cheese , giving certain ”farm house”

    flavor notes to the cheese, due to the culture’s contribution to catabolism of amino -acids. The culture also gives a yellowish color to the

    cheese rind.

    Culture composition Culture Performance

    Growth speed:

    Contribution to coating:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in freeze-dried packaging sizes of 10 units.

    Storage: -18°C/0°F

    Freeze-dried

    BL 

    SALSA 

    Storage: -18°C/0°F

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     Ripening Cultures

    Flavor Control™: Ripening Cultures for propionic, continental and cheddar types of cheeses

    The cultures below are anaerobic ripening cultures recommended for continental, cheddar and propionic cheese. However, in some cases, it

    can also be used in soft cheeses and pasta filata. The cultures have been selected for their flavoring properties, such as proteolytic metabolism

    as well as for their ability to catabolise amino-acids. Their development according to cheese environmental parameters has also been taken

    into consideration.

    PS & Flavor Control™  

     C  u l   t   u r  e C  o m p o s  i   t   i   o n

     

    DVS® PS

    DVS® LH/

    EMFOUR

    DVS®

    CR-200-300

    DVS® 

    CR-520

    CR-501

    DVS®

    CR-520

    CR-501

    DVS®

    CR-540

    DVS®

    CR-550

    DVS® CR-

    Mature01

    DVS® CR-

    Bouquet01

    DVS® CR-

    Buttery01 

    Sweet and

    lipolytic

    Sweet and

    nutty

    Debittering

    and mildMature

    Mature and

    Tutti fruityButtery

    Flavor Profile 

    LbH 

    LbP O  LbH 

    LbL O  LbH 

    O  LbP 

    PAB 

    LbP 

    LbL 

    Lb pl  Lb cu  P ac 

    Lb jo 

    Lb rh 

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     Ripening Cultures

    DVS® PS

    This series is a single strain species of Propionibacterium freudenreichii subsp. freudenreichii  or subsp. shermanii . The cultures are not able to

    acidify, but will ferment lactate and produce propionate, acetate and carbon dioxide. Additionally, the cultures provide the final cheese with

    characteristic sweet notes and famous large holes.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in various frozen and freeze-dried packaging sizes.

    DVS® LH/EMFOUR*

    The LH series is based on Latobacillus helveticus. The cultures vary in intensity of proteolysis and flavor. They enhance cheese maturity and

    provide various flavor notes to the cheese, especially sweet and nutty. Moreover, LH cultures are galactose positive and contribute to reduce

    residual sugars in the cheese curd, avoiding secondary fermentation or browning by Maillard reactions.

    *EMFOUR use is restricted and cannot be used in Gouda/Edam cheeses.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in standard frozen form

    Storage: -45°C/-49°F

    Frozen

    PAB 

    LbH 

    Storage: -45°C/-49°F

    Frozen

    Storage: -18°C/0°F

    Freeze-dried

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     Ripening Cultures

    DVS® Flavor Control™ / CR-200-300

    This series consists of homofermentative mesophilic O culture strains which are lactose negative or slow lactose fermenting, but with a very

    high aminopeptidase activity. The cultures give very mild flavor notes to the finished cheese and can remove cheese bitterness. Lastly, the

    cultures can be used in any type of cheese, but they need to be used in combination with an acidifying culture.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in standard frozen packaging sizes.

    DVS® Flavor Control™ CR-500 / CR-Full Flavor

    These series are made of ripening O culture strains blended with various species and strains of Lactobacilli. The cultures are both able to

    reduce bitterness and build up intense and complex cheese flavors, e.g. fruity, sweet, nutty, caramelized, and savory. All cultures are able to

    increase cheese maturity. Additionally, CR-500 cultures are particularly devoted to low fat cheeses but can also be used in full fat cheeses,

    however, all cultures need to be used in combination with an acidifying culture.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in frozen form in cartons.

    Storage: -45°C/-49°F

    Frozen

    Lb rh Lb cu Lb pl Lb jo P ac

    O  LbL  LbP 

    Storage: -45°C/-49°F

    Culture composition

    Culture composition

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     Protective Cultures

    Introduction to Protective Cultures 

    DVS® BioSafe™ is Chr. Hansen’s response to gram positive spoilages, e.g. Bacillus and Clostrium, for cheese manufacturers who want to go

    natural. BS-10 can improve bio-control during the process and provide more homogeneous products with improved quality of the final cheese.

    BS-10 has a controlled production of nisin that inhibits microbial growth. This culture should be used in combination with a DVS® acidifying

    starter culture.

    DVS® FreshQ® is Chr. Hansen’s way of utilizing the power of nature to inhibit growth of yeasts & molds that reduce shelf-life and negatively

    impact the quality of many dairy products. Sorbic acid and it’s calcium, sodium and potassium salts or natamycin are means to prevent

    unwanted yeast and mold – but it is not in line with consumer trends of natural and clean labels. effective in preventing unwanted yeast and

    mold - but it’s not in line with consumer trends of natural and clean labels. DVS® FreshQ® consists of natural microorganisms with the

    potential to inhibit unwanted contaminants like yeast and mold through fermentation. DVS® FreshQ® cultures add an additional hurdle to

    your quality assurance program and enhances the effectiveness of your sanitation program – with no negative flavor impact.

    Other effect preventing

    yeast and moldDVS® FreshQ®

     S  p e c  i   a l   P  r  o p e r  t   i   e s 

    Nisin production DVS® BS-10

    Culture Composition 

    DVS® BS-10

    This O culture ProtectIT/BS-10 produces nisin that inhibits microbial growth, and thereby extends shelf life for some products. More

    specifically the ProtectIT/BS-10 show antimicrobial activity against several Gram-positive bacteria, including spore-formers such as Clostridium

    and Bacillus subsp.

    Culture composition Culture Performance

    Acidification speed:

    Phage robustness:

    Contribution to flavor:

    Gas production:

    Product Form

    This series is available in frozen form.

    Storage: -45°C/-49°F

    Frozen

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     Kosher For Passover

    Introduction to KFP™ 

    All Chr. Hansen cheese cultures are Kosher certified, however, several other cultures are also certified special Kosher for Passover  (KFP™). The

    figure below provides an overview of the different cultures in the Chr. Hansen KFP ™ series that all have been certified Kosher for Passover . All

    these cultures have similar performance as the regular versions. The specific product KFP™ CHEESE is similar to that of the Fresco® series, and

    therefore, KFP™ CHEESE can be used as an alternative to Fresco® cultures, when Kosher for Passover  is required.

     S  p e c  i   a l   C  h  a r  a c  t   e r  i  

     s  t   i   c 

    Kosher for Passover

    certificate availableKFP™ CH-N-19

    KFP™ R-604

    KFP™ R-608KFP™ CHEESE KFP™ LH-17 KFP™ PS-1 KFP™ STC-7

    Culture Composition 

    The KFP™ cultures are available in standard 500 U frozen packaging sizes, except from the KFP™ CHEESE which is available in 1000 U bag.

    O  O 

    ST 

    LbH  ST PAB LD 

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    Our Product Range & How to use it

    The names of all our cultures in our range are listed in the coming pages. Besides from the name there is

    also information about Culture Type, Culture Performance, Product Form and the Item Number  that is

    used when ordering the culture.

    Culture Name

     All our cultures have individual names. Usually, there are several individual cultures

    e.g. R-603, R-604, R-607, etc. within a certain culture series e.g. the R-600 series.

    Ideally, the user will frequently switch