2015-16 m.sc. part-ii analytical chemistry - …apps.nmu.ac.in/syllab/science and...

1

NORTH MAHARASHTRA UNIVERSITY

JALGAON

Syllabus

for

M. Sc. Part-II

Analytical Chemistry

(Semester III and IV)

With Effect From June 2015

2

M. Sc. Part – II Analytical Chemistry

Course Structure

1) There are in all four theory courses for third semester, three theory courses for fourth semester

and three annual practical courses.

2) The marks for each paper are distributed as : External – 80 marks and Internal – 20 marks.

3) Each theory course is to be completed in 60 lectures.

4) Each theory course should be covered in 6 periods each of 60 minutes per week. Four periods

for lectures and two periods for tutorials, seminars etc.

5) Each practical will require 6 hours of laboratory work per week. Course will be extended over

two semesters and the candidate will be examined at the end of year.

6) There should not be more than 10 students in a batch of M. Sc. Practical Course.

7) Once in a year a visit to Chemical Industry /Research Institute /Science & Technology

Institute is compulsory.

8) The distribution of courses will be as follows:

Semester III

CH – 391 : Concepts of Analytical Chemistry

CH – 392 : Modern Separation Science

CH – 393 : Instrumental Methods of Analysis

CH – 380 : Pharmaceutical Analysis

OR

CH – 381 : Analysis of Organics and Medicinal

Semester IV

CH – 491 : Spectroscopic Methods of Analysis

CH – 492 : Special Analytical Methods and Analysis of Complex Materials

CH – 480 : Environmental Analysis

OR

CH – 481 : Bio-Analysis and Analysis of Food

Practical Courses (Annual)

CH: A-1 : Analytical Chemistry Practical Course – I

CH: A-2 : Analytical Chemistry Practical Course – II

CH: A-3 : Analytical Chemistry Course – III (A Short Research Project and

Industrial Visit Report)

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North Maharashtra University, Jalgaon

M. Sc. II – Analytical Chemistry

Revised syllabus in Analytical Chemistry with effect from June 2015

Course Structure, No of Lectures for each chapter and External Examination marks

weightage:

Semester – III CH – 391 : Concept of Analytical

Chemistry

Sr. No.

Name of the Chapter No. of

lectures

Marks

without

option

Marks with

option

1 Analytical Science – A perspective 10 15 20

2 Good Laboratory Practice: Quality

Assurance of Analytical Measurements

12 15 25

3 Simple D. C. and A. C. Electric

Circuits

12 15 25

4 Introduction to Digital Computers 12 15 20

5 Decomposition and Dissolution of

Inorganic Samples

08 10 15

6 Decomposition and Dissolution of

Organic Samples

08 10 15

TOTAL 60 80 120

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Skeleton for Question Paper

CH – 391 : Concept of Analytical Chemistry

Time Allowed: Three Hours Marks: 80

1. Answer any four of the following: (20)

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e)

f)

2. Attempt any four of the following: (20)

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NORTH MAHARASHTRA UNIVERSITY, JALGAON

Syllabus for M. Sc. Part-II Analytical Chemistry (Semester III and IV)

With Effect from June 2015

CH – 391 : Concept of Analytical Chemistry

1. Analytical Science – A perspective [10L]

History of Analytical Chemistry, Basic concepts, Classical and Instrumental methods, Chemo

metrics, Automated Analysis, Future trends in Analysis.

2. Good Laboratory Practice: Quality Assurance of Analytical Measurements [12L]

Good Laboratory Practice, Validation of Analytical Methods, Quality Assurance, Laboratory

Accreditation, Electronic Records and Electronic Signatures: 21 CFR, Part 11, Official

Organization.

3. Simple D. C. and A. C. Electric Circuits [12L]

D.C. Circuit, Current and Potential measuring devices, resistor, capacitor, transformer, diode

and transistor, A.C. Circuits, Problems.

4. Introduction to Digital Computers [12L]

Introduction to I.C., Operational Amplifier. Micro Processor, Gates and Boolean Algebra,

Digital Computers, Problems.

5. Decomposition and Dissolution of Inorganic Samples [08L]

Introduction, Acids as solvent, oxidizing and non-oxidizing, alkaline fluxes and acid fluxes, Use

of Chlorine and Bromine at high temperature.

6. Decomposition and Dissolution of Organic Samples [08L]

Introduction, Dry and wet ashing methods, Specials reagents, Peroxide bomb, Alkali metal

fusion, Lamp method, Lisbeg method.

REFERENCES

1. Standard Methods of Analysis (Volume Two Part – A) – F. J. Welcher.

2. Introduction to Instrumental Analysis – R. D. Braun.

3. Treatise on Analytical Chemistry (Volume Two Part I) – I. M. Kolthof.

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weightage:

Semester – III CH – 392 : Modern Separation Science

Sr. No.


lectures

Marks

without

option

Marks with

option

1 Chromatography: General Principles 12 15 25

2 Gas Chromatography 12 15 25

3 Liquid Column Chromatography 10 15 20

4 High Performance Liquid

Chromatography

10 15 20

5 Membrane-Based Methods 16 20 30

TOTAL 60 80 120

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1. Chromatography: General Principles [12L]

Theory, Behavior of solutes, Column efficiency and band broadening, Resolution, Column

process, Reduced variable, Time of analysis, Qualitative & quantitative analysis, Problems.

2. Gas Chromatography [12L]

Optimization of experimental condition, Retention time and Retention volume, Detectors:

Thermionic, Flame photometric, Helium and Coulson conductivity detectors, Supercritical fluid

chromatography, Qualitative and Quantitative analysis, Problems.

3. Liquid Column Chromatography [10L]

Introduction, Solvent delivery system, Sample injection, Separation columns Detectors,

Optimization of column performance, Problems.

4. High Performance Liquid Chromatography [10L]

A) Adsorption HPLC, Partition HPLC, Ion Exchange HPLC, Exclusion HPLC, Problems

B) Supercritical Fluid Chromatography, Properties of supercritical fluid, Instrumentation and

operating variables, Applications

5. Membrane-Based Methods:- [16L]

Dialysis-working of techniques, membranes, generalconsideration of diffusion, Donnan

Membrane equilibrium and Applications.

Electrodialysis- working of techniques, membranes, Electrodialysis cells and Applications.

Ultrafiltration- working of techniques, membranes, non-gelatinous membranes and Applications.

Dialysis compared with other membrane-separation methods.

Other Separation Methods:-Ultracentrifugation-Principle, sedimentation constant, sedimentation

equilibrium, sedimentation velocity, methodology and applications.

Zone refining- Principle, zone leveling and applications.

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REFERENCES

1. Instrumental Methods of Chemical Analysis (6th Ed.) – H. H. Willard, L. L. Merrit, J. D.

Dean, S. A. Settle, Jr. 10


3. Introduction to Separation Science – L. R. Synder & H. O, Hawarth.

4. A Textbook of Inorganic Qualitative Analysis. By A I Vogel.

5. Chromatography. By E Heftman, 5th edition, part-A and part-B, Elsewhere Science

Publisher, 1992

6. Chromatography Today. By C F Poole and S K Poole, Elsewhere Science Publisher, 1991

7. Analytical Chemistry. By G D Christian 4th edition, John Wiley and Sons

8. Instrumental Methods of Analysis, by B K Sharma, 19th edition, Goel Pubisher,2000

9. Basic Concept in Analytical chemistry, by S.M. Khopkar

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weightage:

Semester – III CH – 393 : Instrumental Methods of

Analysis

Sr. No.


lectures

Marks

without

option

Marks with

option

1 Electrogravimetry 12 15 25

2 Voltammetry & Coulometry 12 15 25

3 High Frequency Titrations 08 10 15

4 Automated Analysis 16 25 30

5 Polarimetry 12 15 25

TOTAL 60 80 120

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1. Electrogravimetry [12L]

Electro separations, Basic principles, Equipments for electrolytic separation, Electrogravimetry,

Electrography, Electrolytic purification, Problems.

2. Voltammetry & Coulometry [12L]

Introduction, principle and technique of voltammetry and coulometry. Pulse polarography, cyclic

voltammetry, Anodic stripping voltammetry, coulometry at constant current and controlled

potential coulometry, applications of volatammetry and coulometry, Problems.

3. High Frequency Titrations [08L]

Introduction, Theory, Instrumentation, Applications, Advantages and disadvantages of HF

titrations, Problems.

4. Automated Analysis [16L]

Automated laboratory analysis, Computerization, Automated laboratory apparatus, Continuous

flow analyzer, Flow injection analyzer, Desecrate sample analyzer, Centrifugal force analyzer,

Automatic titrator, Robots, Process control analyzer.

5. Polarimetry [12L]

Polarisation of light, Plane of vibration, Plane of polarization, Optical activity, Factors affecting

angle of rotation, Specific rotation, Optical rotator dispersion and circular dichroism- Cotton

effect, ORD and CD curves, Instrumentation, Measurement of rotatory power, Theories of

optical activity, Applications of polarimetry, Optical activity and chemical constitution,

Representation of optical isomerism, two structures for a molecule, Differentiation between a

pair of enantiomorphs, Saccharimetry, Difference between saccharimetry and polarimetry,

Saccharimeters, Kinetic polarimetry, Spectropolarimetry

REFERENCES

1. Instrumental Methods of Chemical Analysis (6th Ed.) – H. H. Willard, L. L. Merrit, J. D.

Dean, S. A. Settle, Jr.


3. Instrumental Methods of Chemical Analysis – G. Chatwal & S. Anand.

4. Treatise on Analytical Chemistry (Volume One Part III) – I. M. Kolthoff & P. J. Elwing.

13

5. Fundamentals of Analytical Chemistry – D. A. Skoog, D. M. West and H. J. Holler

6. A Text book of Quantitative Analysis by A.I.Vogel

7. Basic Concept in Analytical chemistry, by S.M. Khopkar.

8. Principles of Instrumental Analysis, Holl Seamlers by D.Sleool and U.West

9. Instrumental Methods of Chemical Analysis, Pragati Prakashan Meerut by H.Kaur

10. Instrumental Methods of Chemical Analysis – B. K. Sharma

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weightage:

Semester – III CH – 380 : Pharmaceutical and Cosmetic

Analysis

Sr. No.


lectures

Marks

without

option

Marks with

option

1 Pharmaceutical Industry: a review 10 15 20

2 Official Methods of Standardization

and Quality Control

10 15 25

3 Assay and Tests for Drugs 20 25 35

4 Introduction to Cosmetics 08 10 15

5 Analysis of Cosmetics 12 15 25

TOTAL 60 80 120

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CH – 380 : Pharmaceutical and Cosmetic Analysis



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CH – 380 : Pharmaceutical and Cosmetic Analysis

1. Pharmaceutical Industry: a review [10 L ]

Definition and classification of drugs and medicines, introduction to pharmaceutical

formulations, classification of dosage forms, Sources of impurities in pharmaceutical chemicals

and raw materials.

2. Official Methods of Standardization and Quality Control [10 L]

Standardization of finished products and their characteristics, Official methods of Quality

control, Dissolution Tests and Kinetics.

3. Assay and Tests for Drugs [20 L ]

Analysis of compounds based on functional groups, instrumental methods for analysis of drugs,

assays involving chromatographic separations, proximate assays, assays of enzyme containing

substances, biological and microbiological assays and tests. Limit tests, solubility tests,

disintegration tests, stability studies, impurity profile of drugs, bioequivalence and bioavailability

studies.

4. Introduction to Cosmetics [08 L]

Cosmetics: Introduction, Evaluation of cosmetic material and raw material and additives,

Formulation, standards and methods of analysis.

5. Analysis of Cosmetics [12 L]

Deodorants and antiperspirants: Al, Zn, Zr, Boric acid, chlorides, sulphates, hexachlorophene,

methanamine, phenolsulphonates and urea

Face powder: Fats, fatty acids, boric acid, Ca, Mg, BaSO4, Ti, Fe, oxides of Tl, Fe and Al (total).

Hair tonic: 2,5-diaminotoluene, potassium bromates, sodium perborate, pyrogallol, resorcinol,

salicylic acid, dithioglycollic acid (in permanent wavers)

Creams and lotions: types of emulsions, chloroform soluble material, glycerol, pH emulsion, ash

analysis, non volatile matter by IR spectroscopy.

Lipsticks: General analysis, determination of nonvolatile matter, ash analysis determination of

lakes and fillers, trichloroethylene – acetone soluble contents.

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REFERENCES

1. Analytical Biochemistry, D, J. Homes and H. Peck, Longman (1983)

2. Bioanalytical Chemistry, S. R. Mikkelesen and E. Corton, John Wiley and sons 2004

3. Analysis of food and beverages, George Charalanbous, Accademic press 1978.

4. Harry’s Cosmetology, Longman scientific co.

5. Formulation and Function of cosmetics, Sa Jellineck.

6. Cosmetic Technology, Saggarin.

7. Modern cosmetics, E. Thomessen Wiley Interscience.

8. Encyclopaedia of industrial chemical analysis, Snell et al Inter science.

9. Govt of India publications of food drug cosmetic act and rules.

10. Hand book of drug law, Mehta Univ. Book agency Ahmedabad.

11. Chemical analysis of drugs, Higuchi, Interscience, 1995.

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weightage:

Semester – III CH – 381: Analysis of Organics and

Medicinal

Sr. No.


lectures

Marks

without

option

Marks with

option

1 Determination of Hydrocarbons,

Carbonyl Compounds, Nitrogen

Compounds, and Sulphur Compounds

14 20 30

2 Analysis of petroleum products 10 15 20

3 Analysis of Polymers and Plastics 10 10 15

4 Analysis of Agrochemicals 12 15 25

5 Analysis of Medicinal & Drugs 14 20 30

TOTAL 60 80 120

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CH – 381: Analysis of Organics and Medicinal



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CH – 381: Analysis of Organics and Medicinal

1. Determination of Hydrocarbons, Carbonyl Compounds, Nitrogen Compounds, and

Sulphur Compounds [14L]

a) Hydrocarbons – Carbon & Hydrogen by combustion method.

b) Carbonyl compounds – Estimation by oxidation, Bisulphate addition, Gravimetric DNP

method & saponification method.

c) Nitrogen compounds – Estimation by Kjeldahl & Duma’s methods, Aromatic amines by

diazotization & nitrosation, Reduction method for azo, hydrazo & nitro compounds by titanous

chloride method. Sulphur compounds – Oxygen flask method, Bregel method, Mercaptans by

silver sulphate method.

d) Numerical problem on above method

2. Analysis of petroleum products [10L]

Cloud point, pour point, flash point, vapour pressure, specific gravity and their determination,

Estimation of ‘S’ and ‘Pb’ in petrol, octane number, cetane number

3. Analysis of Polymers and Plastics [10L]

a) Introduction to rubber products isopropene polymers.

b) Plastics identification by IR & GC method for ethylene plastics, styrene plastics, vinyl

chloride plastics and acrylic plastics.

4. Analysis of Agrochemicals [12L]

Introduction, Classification, mechanism of action and synthesis.

Insecticides : Malathion, Parathion.

Herbicides : Banalin, Butacarb .

Fungicides : Boardeaux mixture, Copper oxychloride

Analysis of pesticide residue and toxicological effects.

5. Analysis of Medicinal & Drugs [14L]

a) Analysis of Medicinals such as Asprin, Sulpha drug, Assay of drugs based on functional

groups as amino, -SH and acidic group.

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b) Assay of Vitamin such as Vitamin A, Vitamin B, Vitamin B2, Vitamin B6, Vitamin B12,

Vitamin C, Vitamin D, Vitamin E.

c) Enzyme assay methods – Ratio amino assay and ELISA method.

REFERENCES

1. Standard Methods of Chemical Analysis (Vol. Two Part A) – F. J. Welcher.

2. Standard Methods of Chemical Analysis (Vol. Two Part B) – F. J. Welcher.

3. Standard Methods of Chemical Analysis (Vol. Three Part B) – F. J. Welcher.

4. Analytical Bio-Chemistry – Holme & Peck.

5. British Pharmacopoeia

6. Forensic Pharmacy of Ethics

7. Encyclopedia of Analytical Chemistry Vol. 3. Academic Press 1995

8. Principles of polymer systems by F. Rodrigue, Tata Mc Graw Hill, New Delhi.

9. Principles of polymer systems by P.J.Flory, Cornell University press, New York

10. Polymer chemistry-an introduction Seymour-Carraher-Marcel Dekker. Inc

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weightage:

Semester – III CH – 491: Spectroscopic Methods of Analysis

Sr. No.


lectures

Marks

without

option

Marks with

option

1 UV-Visible & Infra - Red

Spectroscopy

08 10 15

2 NMR 10 15 20

3 Atomic Mass Spectroscopy 12 15 25

4 Atomic Fluorescence, Resonant

Ionization and laser based-Enhanced

Ionization

12 15 25

5 Coupled Techniques 10 15 20

6 Principle, Instrumentation &

Analytical Applications of ESCA and

mossbauer spectroscopy

08 10 15

TOTAL 60 80 120

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CH – 491: Spectroscopic Methods of Analysis



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CH – 491: Spectroscopic Methods of Analysis

1. UV-Visible & Infra - Red Spectroscopy [08L]

Principle of UV visible and Infrared.Spectometry. Instumentation. Sasmple handling. Qualitative

and Quantitative analysis. Problems.

2. NMR [10L]

Theory of NMR-Quantum description, classical description of NMR, Relaxation Processes in

NMR. Environmental effects on NMR Spectra-Chemical shift, spin splitting. Rules governing

the interpretation of first order spectra. Effect of chemical exchange. NMR Spectrometers

Applications of proton NMR, c-13 NMR.

3. Atomic Mass Spectroscopy: [12 L]

Features of atomic mass spectroscopy, Atomic weight in mass spectroscopy, mass to charge

ratio, Types of atomic mass spectroscopy, mass spectrometers, transducer for mass spectroscopy,

qudrapole mass analyzer, time of flight mass analyzer, Inductively coupled mass spectroscopy

(ICPMS), Instrumentation for ICPMS, Atomic mass spectra and interferences, Applications of

ICPMS

4. Atomic Fluorescence, Resonant Ionization and laser based-Enhanced Ionization: [12L]

Atomic Fluorescence Spectroscopy (AFS): Atomic fluorescence, apparatus for AFS, EMR

source for AFS, LASERS, Cells for AFS, Plasmas, Wavelength selection for AFS, Detectors for

AFS, Theory of AFS, Analysis with AFS, Interference With AFS.

Resonanant Ionization Spectroscopy

Laser-enhanced ionization spectroscopy

5. Coupled Techniques [10L]

Combination of two analytical tools such as GC-MS, HPLC-MS, GC-FTIR for chemical analysis

in broader context and enhanced accuracy in structure predictions/ quantifications

6. Principle, Instrumentation & Analytical Applications of ESCA and mossbauer

spectroscopy [08L]

Principle, Instrumentation & Analytical Applications of ESCA & Mossbauer spectroscopy

25

REFERENCES


2. Instrumental Methods of Analysis – H. H. Willard & L. L. Merritt.

3. Instrumental Methods of Chemical Analysis – G. W. Ewing.

4. Analytical Chemistry- G. D. Christain

5. Fundamentals of Analytical Chemistry – D. A. Skoog, D. M. West and H. J. Holler

6. Practical Aspects of Gas Chromatography/Mass spectroscopy – G. M. Message, John Wiley &

Sons

7. Instrumental Methods of Chemical Analysis – G. Chatwal & S. Anand.

8. Organic Spectroscopy – P. S. Kalsi

9. Spectroscopic Methods in Organic Chemistry – D. H. Williams and I. Fleming; McGraw hill

10. Application of Absorption spectroscopy of Organic Compounds – J. R. Dyer, Prentice hall

11. Analytical Chemistry – Kelmner

12. Chemical Analysis- Farm cis, Roucssal and A mick Roucssal

15) Principles of Instrumental Analysis, Skoog, Holler, Nieman

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weightage:

Semester – III CH – 492: Special Analytical Methods & Analysis

of Complex Materials

Sr. No.


lectures

Marks

without

option

Marks with

option

1 Radiochemical Methods of Analysis 12 15 25

2 Gas Volumetric Methods of Analysis 12 15 25

3 Analysis of Pigments (tinting strength,

reducing power)

10 15 20

4 Analysis of Minerals & Ores Such As; 12 15 20

5 Analysis of Alloys 14 20 30

TOTAL 60 80 120

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CH – 492: Special Analytical Methods & Analysis of Complex Materials



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CH – 492: Special Analytical Methods & Analysis of Complex Materials

1. Radiochemical Methods of Analysis [12L]

Measurement of Radio activity – Scintillation counter, Sample preparation, Mounting, Neutron

Activation Analysis (NAA), Isotope Dilution Analysis (IDA), Problems.

2. Gas Volumetric Methods of Analysis [12L]

Apparatus employed in gas analysis – Orsat, Bunt and Hampel apparatus, Absorbents for various

gases, Determination by explosion, slow explosion and fractional combustion, Problems.

3. Analysis of Pigments (tinting strength, reducing power) [10L]

Inspection and separation of solvent thinnable and waterborne coatings into polymeric binders

and pigments. Analysis of solvents (flash point, evaporation rate, boiling range, auto ignition

temperature, solubility parameter.

4. Analysis of Minerals & Ores Such As; [12L]

Monazite, Bauxite, pyrolusite, hematite, Ilmenite

5. Analysis of Alloys [14L]

Brass, Bronze, Steel and Silver Alloys

REFERENCES

1. A Text Book of Quantitative Inorganic Analysis (3rd Edition) – A. I. Vogel.

2. Standard Methods of Chemical Analysis (Vol. Two Part B) – F. J. Welcher.

3. Treatise on Analytical Chemistry (Series of Volumes) – I. M. Kolthoff & P. J. Elwing.


5. Handbook of Industrial Chemistry – Davis Burner

4. Soil and Sediment Analysis

5. Outline of Paint Technology - Morgan

6. Organic Coating Science and Technology – Pappas and Jones

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weightage:

Semester – III CH –480: Environmental Analysis

Sr. No.


lectures

Marks

without

option

Marks with

option

1 Environment 10 10 15

2 Air Pollution Analysis 14 20 30

3 Water Pollution Analysis 14 20 30

4 Soil and Sediment Analysis 14 20 30

5 Hazardous Waste Analysis 08 10 15

TOTAL 60 80 120

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CH –480: Environmental Analysis



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CH –480: Environmental Analysis

1. Environment [10L]

Environment: Definition, characteristics and pollution, types of Environmental pollution,

Environmental pollutants: Metallic and non-metallic, Toxic chemicals: heavy metals, Pesticides

and carcinogenic compounds.

2. Air Pollution Analysis [14L]

Air pollution: Definition and major air pollutants Air pollution monitoring: sample collection and

Estimation of SOx, NOx, CO, hydrocarbons, and particulate matter, Air quality standards:

ambient and stack.

3. Water Pollution Analysis [14L]

Water pollution: Inorganic and organic, Water quality parameters, Analysis of physicochemical

parameters :pH, Conductivity , turbidity, DO, COD, BOD, TS, TDS, TSS, analysis of biological

parameters: MPN and SPC, trace element analysis, agricultural and industrial wastewater,

drinking water standards, standards for waste water disposal.

4. Soil and Sediment Analysis [14L]

Sediment and soil sampling, Soil and sediment pollutants, Analysis of trace PCBs, PAHs and

heavy metals, Analysis of fertilizer and pesticides residues, Analysis of plant macronutrients

such as N, P and K and micronutrients such as B, Mo, Mn, Mg, etc., Estimation of sodium and

CEC.

5. Hazardous Waste Analysis [08L]

Hazardous wastes, hazardous waste management: characterization, collection, transport and

disposal, hazardous waste management rules.

REFERENCES

1. Standard Methods of Chemical Analysis (Relevant Volumes)- F. J. Welcher

2. Standard Methods of Water and Waste Water Analysis –A. K.De.

3. Environmental Chemistry –S. M. Khopkar.

4. Environmental Chemical Analysis –M. S. Cress & Morr.

5. Analytical Chemistry- G. D. Christian

32





weightage:

Semester – III CH – 481: Bio – Analysis & Analysis of Food

Sr. No.


lectures

Marks

without

option

Marks with

option

1 Human Nutrition, Biological Value of

Food

12 15 20

2 Food Analysis 12 15 25

3 Determination of food preservatives 14 20 30

4 Collection of Specimens and Analysis

of Blood and urine

12 15 25

5 Forensic Analysis 10 15 20

TOTAL 60 80 120

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CH – 481: Bio – Analysis & Analysis of Food



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CH – 481: Bio – Analysis & Analysis of Food

1. Human Nutrition, Biological Value of Food [12L]

Human nutrition, Biological value of food, Estimation of carbohydrates, Fats, Proteins, Amino

acids.

2. Food Analysis [12L]

Analysis of food: Milk and milk products, Tea, Coffee, Flour, Starch, Honey, Jam, Oils Analysis

of food related materials: Preservatives, Coloring matter, trace metals, pesticide residues

3. Determination of food preservatives [14L]

Definition, SO2 legislation and determination by Tanners method, Nitrate and nitrites legislation

and determination, boric acid legislation and determination, Benzoic acid legislation and

determination, 4-hydroxybenzoate legislation and determination, ascorbic acid legislation and

determination. Sweeteners: Saccharine identification and determination, Colours: Identification

by general methods, Natural colours.

4. Collection of Specimens and Analysis of Blood and urine [12L]

Blood: Collection of Blood specimens, storage and preservation, Urine: Collection of Urine,

physical characteristics of urea, preservation and storage, Faeces: Collection and preservation.

Determination of blood and plasma glucose by glucose oxidase method, Determination of urine

for glucose, Determination of ketone bodies in blood, Oral Glucose tolerance test, Determination

of serum creatinin, estimation of serum bilirubin, Estimation of serum cholesterol, determination

of blood hemoglobin, Urate: determination of serum urate, determination of urea in urine by

urease method and by direct colorimetry, Estimation of Na, K, Ca by flame photometry,

inorganic phosphate by colorimetry.

5. Forensic Analysis [10L]

Introduction, Forensic examination of biological fluids, stains semen, hair, other

materials,Forensic toxicology, Techniques used in molecular probing – southern,

northern, western, blotting, DNA fingerprinting.

REFERENCES

1. Biochemistry – Lehninger.

2. Physiological Analytical Biochemistry (2nd Edition) – Holme & Peck.

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3. Pearson’s chemical analysis of food

4. A Manual of Laboratory Techniques –National Institute of Nutrition, I. C. M. R. Hyderabad.

5. Standard Methods of Chemical Analyis (Vol. Three Part A) – F. J. Welcher.

6. Essentials of Food and Nutrition, Vol. I and II – M. Swaminathan, Bangalore Printing &

publishing Co. Ltd

7. Hand Book of Food Analysis – S. N. Mahindru.

8. Medical Laboratory Techniques – Dr. Ranik Good.

9. Practical Clinical Biochemistry – Harold Warely.

10. Forensic Pharmacy & Ethcs – V. N. Joshi & M. R. Varad.

11. Text Book of Biochemistry – G. R. Chatwal.

12. Practical Clinical Biochemistry, Gowenlock, CBS published, 6th Ed.

13. Forensic Chemistry by Suzanne Bell, Pearson Prentice Hall Publishers, 2006

36

ANALYTICAL CHEMISTRY PRACTICAL COURSE

CH: A-1: Analytical Chemistry Practical Course – I

Perform any 15 experiments out of the following:

1. Determination of Na/K/Li in given sample by Flame photometry method.

2. Determination of Ca in egg shell by Flame photometry method.

3. Determination of p-nitrophenol by colorimetry.

4. Determination of water in organic sample by Karl – Fischer titration.

5. Estimation of O2 and CO2 in air by Orsat method.

6. Determination of strength of acetic acid in vinegar by conductometry titration.

7. Determination of Asprin in drug tablet by pH metry titration with NaOH.

8. Determination of Commercial washing soda by potentiometric titration method.

9. Determination of composition of complex by Job’s continuous variation method.

10. Quantitative analysis of Iron, Calcium and Phosphorus in Milk powder. (Fe-Colorimetrically,

Ca – Complexometrically, P – Colorimetrically)

11. Casein isolation from milk by isoelectric precipitation (Yield expected).

12. Determination of available phosphorus in soil Olesen’s colorimetric method.

13. Determination of viscosity of ointment/syrup/oils (minimum 3 samples) using Brookfield

viscometer.

14. Determination of Phosphate in Detergents by Spectrophotometry

15. Spectrophotometric determination of inorganic phosphorous in human serum or urine.

16. Determination of SO42-

and Cl- by turbidimetric method.

17. Determination of purity of sugar sample by optical rotation by polarimetry.

18. Method development of Drug by HPLC.

19. Determination of heavy metals from industrial waste water using AAS.

20. Separation and identification of plasma proteins by paper electrophoresis

method.

37

CH: A-2 : Analytical Chemistry Practical Course – II

Perform any 15 experiments out of the following:

1. Analysis of alloy or ore (any one)

a. Analysis of Nichrome alloy: i) Cr by colorimetry ii) Ni by gravimetry

b. Analysis of Zinc blend ore i) Zn by complexometry ii) Fe by volumetry

c. Analysis of Calcite ore i) Ca by complexometry ii) Fe by colorimetry

2. Determination of COD value of given sample of polluted water.

3. Separation and estimation of Fe and Al on cation exchanger resin.

4. Determination of total chlorine in pesticide formulation.

5. Determination of copper in fungicide.

6. Determination of available chlorine in bleaching powder by Bunsen method.

7. Estimation of ascorbic acid by cerric ammonium sulphate method..

8. Estimation of mixture of benzoic acid and salicylic acid in pharmaceutical preparation.

9. Isolation of tannin or caffeine in tea

10. Determination of blood Glucose by Folin-Wu method

11. Estimation of Glucose and fructose in honey by Lane and Eynone method.

12. Estimation of amino acid in protein by Sorensen formal titration method.

13. Estimation of lactose in milk by iodometry.

14. Seperation of Amino acid by Two dimensional chromatography

15. Molecular weight of polymer from viscocity measurement

16. Estimation of Ca in Ca-pentathonate/Cl-lactate tablet

17. Estimation of aldehyde in lemon /cinnamon oil

18. Separation and determination of Co & Ni using basic anion exchange resin Dowex.

19. Determination of organic carbon in soil by Walkley and black method.

20. Isolation of saccharin in beverages

38

REFERENCES FOR PRACTICAL

1. A Text Book of Qualitative Inorganic Analysis (3rd Edition) – A. I. Vogel

2. Standard Methods of Chemical Analysis (A Series of Volumes) – F. J. Welcher.

3. Treatise on Analytical Chemistry (A Series of Volumes) – I. M. Kolthoff & P. J. Elwing.

4. Association of Official Analytical Chemistry (AOAC) – 13th Edition 1980.

5. Instrumental Methods of Analysis (6th Edition) – H. H, Willard & L. L. Merritt

6. Pharmacopoeia of India, British & United States.

7. Hand Book of Food Analysis – S. N. Mahindru.

8. Analytical Biochemistry – Holme Peck

9. Post Graduate Chemistry Practical Part – I – Patel, Gadre & Turkhia. 38

10. Environmental Chemistry by A.K.De 3rd

edition Wiley Eastern Ltd.

11. Experiments in Chemistry by D.V.Jahagirdar (Himalaya publication)

CH: A – 3 A Short Research Project and Industrial Visit Report

a. Project Report (should include Introduction, objective, Methodology/Technique, Results and

Discussion, Conclusions & References)

b. Industrial Visit Report

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