2019 midwest association of veterinary pathologists pre ... · 2019 meeting of the midwest...

2019 Midwest Association of Veterinary Pathologists

Pre-Meeting Program

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2019 MEETING OF THE MIDWEST ASSOCIATION OF VETERINARY PATHOLOGISTS PARKE COUNTY, INDIANA

Number Case # Presenter File name 1 H3981 Shelley Newman 01_H3981_Newman_MAVP2019 2 1905440 Amy Usborne & Iris

Bolton 02_1905440_Usborne+Bolton_MAVP2019

3 121 Dodd Sledge 03_121_Sledge_MAVP2019 4 18-38351-1 Sarah Zalar 04_18-38351-1_Zalar_MAVP2019 5 9934 Sarah Corner 05_9934_Corner_MAVP2019 05_9934_Corner_MAVP2019-1 6 A19-14209 Mara Varvil 06_A19-14209_Varvil_MAVP2019 7 A19-12451 Stacey Piotrowski 07_A19-12451_Piotrowski_MAVP2019 8 NP-14-1040 Sarah Coe 08_NP-14-1040_Coe_MAVP2019 9 19-35867-2 Natalie Kirk 09_19-35867-2_Kirk_MAVP2019 10 A19-17017 Melissa Swan 10_A19-17017_Swan_MAVP2019 11 Burcham-01 Grant Burcham 11_Burcham-01_Burcham_MAVP2019 11_Burcham-01_Burcham_MAVP2019-1 12 17-H46849-

12 Andrea Pohly 12_17-H46849-12_Pohly_MAVP2019

13 NC-19-575 Leah Stein 13_NC-19-575_Stein_MAVP2019 13_NC-19-575_Stein_MAVP2019-1 14 4217 MSU

VDL Annie Zimmerman 14_4217 MSU VDL_Zimmerman_MAVP2019

14_4217 MSU VDL_Zimmerman_MAVP2019-1

15 n/a Eileen Henderson 15_na_Henderson_MAVP2019 16 190422019 Priscila Serpa 16_190422019_Serpa_MAVP2019 16_190422019_Serpa_MAVP2019-2 17 n/a George Sandusky 17_Before Treatment

Control_Sandusky_MAVP2019 17_OTL Patient 3_Sandusky_MAVP2019 17_Sutent and Inlyta Treatment Combo

5_Sandusky_MAVP2019 18 A19-1484 Caitlin Brown 18_A19-1484_Brown_MAVP2019 19 CBBBl-23 Tiffany Lyle 19_CBBBl-23_Lyle_MAVP2019 20 7980018 Sara Obeiter 20_7980018_Obeiter_MAVP2019 21 AC19-050 Wayne Buck 21_AC19-050_Buck_MAVP2019 22 7679 Kerry Goldin 22_7679_Goldin_MAVP2019 23 S19-1808 Adrianne Glaser 23_S19-1808_Glaser_MAVP2019 24 A19-11134 Jess Hanlon 24_A19-11134_Hanlon_MAVP2019 25 19RD1977 Gillian C. Shaw 25_19RD1977_Shaw_MAVP2019 26 24460 Catherine

Shoemake 26_24460_Shoemake_MAVP2019

27 19-H42694-3 Miranda Vieson 27_19-H42694-3_Vieson_MAVP2019

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28 SP-19-7690 Glenn Brado 28_SP-19-7690_Brado_MAVP2019 28_SP-19-7690_Brado_MAVP2019-1 29 7442-19-1 Rosalie Ierardi 29_7442-19-1_Ierardi_MAVP2019 30 SP-19-4881-

1 Ryan Yanez 30_SP-19-4881-1_Yanez_MAVP2019

31 19-501 Kimberley Sebastian 31_19-501_Sebastian_MAVP2019 32 SP-19-7167 Steven Hsu 32_SP-19-7167_Hsu_MAVP2019 33 3695 Allison Gerras 33_3695_Gerras_MAVP2019 34 1170 Yue (Polly) Chen 34_1170_Chen_MAVP2019 35 NC-19-711 Anna Gates 35_NC-19-711_Gates_MAVP2019 36 DERM-2 Ryan Jennings 20_DERM-2_Jennings_MAVP2019 37 MSU-TT Tuddow Thaiwong 37_MSU-TT_Thaiwong_MAVP2019 38 19-35760-1 Janice Pfeiff 38_19-35760-1_Pfeiff_MAVP2019 39 SP-19-7347 Erica Noland 39_SP-19-7347_Noland_MAVP2019 40 19-37390-8 Patrick Joseph

Roady 40_19-37390-8_Roady_MAVP2019

41 18-1196-4 Allison Mustonen 41_18-1196-4_Mustonen_MAVP2019 42 725452 Mackenzie Long 42_725452_Long_MAVP2019 43 Purdue

University 804-471

Natalia Strandberg 43_Purdue University 804-471_Strandberg_MAVP2019

44 AC18-125 Richard Allan Peterson II

44_AC19-125_Peterson_MAVP2019

45 19 Zachary Millman 45_19_Millman_MAVP2019 46 19N-0191 Erika Hoffeld 46_19N-0191_Hoffeld_MAVP2019 47 A19-10264 Levi Smith 47_A19-102+A1:D5664_Smith_MAVP2019

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Case History:

Case #: H3981

Presenter: Shelley Newman

Corresponding Institution: Long Island University College of Veterinary Medicine

Signalment: 2-year-old FS Domestic Short Hair Cat

History: The cat had a history of chronic intermittent diarrhea in first year of life and chronic intermittent vomiting in second year of life. There had been associated weight loss.

The cat had a severe regenerative anemia. The cat had been on a series of medications including cerenia and doxycycline.

Gross findings: There was severe gastric wall thickening with loss of layering on ultrasound. On endoscopy there was radial mucosal thickening up to 1.3 cm.

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Case History:

Case #: 1905440

Presenters: Iris Bolton; Amy Usborne

Corresponding Institution: Eli Lilly and Company

Signalment: (5) 18 week old female NSG™ mice from Jax

History: Mice were received from vendor 10 weeks previous to being put on report to Veterinary Resources for alopecia and rough hair coat. All mice examined and submitted were housed in the same MACS Gentle-Air Wall Mount Ventilated Caging system and offered Teklad global soy protein-free extruded irradiated chow (2920x) and DI water ad libitum. Animals were monitored over the next two weeks to assess hydration and track clinical progression. Euthanasia was elected due to the failure of the animals’ clinical improvement.

Gross findings: All 5 animals exhibited generalized rough coats and marginal hydration. Patchy alopecia was noted on the head and necks of animals and skin appeared dry and flaky in both haired and non-haired locations. Remaining tissues were unremarkable.

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Case History:

Case #: MSU VDL NC-19-121

Presenter: Dodd Sledge

Corresponding Institution: Michigan State University Veterinary Diagnostic Laboratory

Signalment: 6-year-old castrated male Labrador retriever

History:

This animal had a 2-week history of weakness, inappetance, and a swollen right eye that were treated with Metronidazole, Tobramycin, and Carprofen. On presentation at Michigan State University, the dog was markedly ataxic, but ambulatory. Mentation was dull, and no proprioceptive defects were noted.

Ophthalmic examination revealed buphthalmos OD, hyphema OD, absent menace OD, decreased retropulsion OD, and retinal detachment OS.

Abdominal radiographs identified bilaterally enlarged kidneys and lytic lesions in the left proximal femur and lumbar vertebrae. Chemistry profile revealed marked uremia (urea nitrogen 170mg/dL [12-27mg/dL] and creatinine 5.8mg/dL [0.6-1.5mg/dL]), hypergammaglobulinemia (14.8g/dL [2.3-3.7g/dL]), and hypoalbuminemia (1.4g/dL [2.8-3.6g/dL]). Mild to moderate increases in total bilirubin glucose, ALT, ALP, and AST were also identified.

Gross findings:

On gross examination, the right eye was mildly buphthalmic and had thinning of the limbal and posterior sclera. The anterior and posterior compartments were filled with densely proteinaceous material. The retina was diffusely detached and surrounded by a large amount of free hemorrhage. The subretinal space contained similar, densely proteinaceous fluid and hemorrhage.

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Case History:

Case # 18-38351-1

Presenter: Sarah Zalar

Institution: University of Illinois at Urbana-Champaign

Signalment: 6-week-old, intact female mixed breed dog

History:

This dog presented to the local humane society for lethargy and hypothermia and died suddenly.

Gross Findings:

The heart had multifocal areas of pallor that extended from the epicardium to the myocardium. The lungs were mottled pink to red, and were soft and rubbery. The abdominal cavity contained approximately 10 mL of light yellow to red, clear, thin fluid.

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Case History:

Case #: 9934

Presenter: Sarah Corner, Tuddow Thaiwong, Matti Kiupel

Corresponding Institution: Michigan State University Veterinary Diagnostic Laboratory (MSU VDL)

Signalment: 3-year-old, spayed female, Catahoula dog

History:

This dog had a vaginal mass with intermittent bleeding independent of urination. The mass was surgically removed and submitted to the Michigan State University Veterinary Diagnostic Laboratory for histopathology.

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Case History:

Case #: A19-14209

Presenter: Mara S. Varvil

Corresponding Institution: Purdue University

Signalment: 2-year-old female spayed feline

History: A two-year-old female spayed feline presented to the Purdue University Veterinary Teaching Hospital Emergency Service after being diagnosed with an anemia of unknown etiology by the referring veterinarian earlier in the day. The patient had a four-month history of defecating outside of the litterbox and abnormal behavior that involved licking rocks, metal and other objects. On presentation, it was shown the patient was positive for feline leukemia virus infection based on testing with IDEXX FIV/FeLV Combo.

Table 1 demonstrates the pertinent complete blood count changes at the time of presentation; the bone marrow aspiration and core collection were collected within 24 hours of this CBC.

Table 1. Complete blood count values from the time of presentation (3/28/19) and the time of the bone marrow collection (3/29/19).

Value 3/28/19 Reference Intervals Total Protein 6.8 6.0-8.0 g/dL RBC count 0.89 5.0-10.0 M/ µL

Hematocrit 6.6 30-45%

Hemoglobin 2.2 8.0-15.0 g/dL

MCV 73.8 40-55 fL

MCHC 33 30-36 g/dL

Reticulocyte count 24 <60 K/µL

Erythron comments Rouleaux; 2+ anisocytosis.

WBC 10.2 6-18 K/µL

Segmented Neutrophils

1.9 3-12 K/µL

Bands 0.41 0.00-0.30 K/µL

Lymphocytes 6.8 1.5-7.0 K/µL

Monocytes 0.2 0.05-0.85 K/µL

Eosinophils 0.2 0.1-1.5 K/µL

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Gross findings: None reported.

Basophils 0.1 0.0-0.1 K/µL

Other cells 0.2

WBC comments Other cells are consistent with a population of immature cells. These cells are most consistent with myeloid precursors; they are 20 µL in size with a small to moderate amount of basophilic cytoplasm that occasionally contain scattered azurophilic granules or clear vacuoles. Their nuclei are round with a fine to slightly clumped chromatin pattern with variable prominent nucleoli. Rarely blasts had deeply basophilic cytoplasm and condensed chromatin, more consistent with erythroid precursor cells, are seen.

Platelets Adequate, clumped 200-500 K/µL

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Case History:

Case #:

A19-12451

Presenter:

Stacey Piotrowski, DVM

Anatomic Pathology Resident, NIH Comparative Biomedical Scientist Training Program Fellow

Corresponding Institution:

Purdue University

Signalment:

8.5-year-old, female spayed, Boxer dog

History:

The dog presented to the Indiana Animal Disease Diagnostic Laboratory for necropsy after owners elected euthanasia due to recent clinical decline. The animal had a 6-8-month history of dull mentation and an approximately 1-month history of seizures and circling to the left. Previously diagnosed clinical conditions included Cushing’s disease (hyperadrenocorticism), hypothyroidism, systemic hypertension, tricuspid valve dysplasia, and ventricular premature complexes (VPCs).

Gross findings: When the cerebrum is incised on the sagittal plane, an approximately 1.5 cm x 0.8 cm x 0.5 cm discrete, mottled yellow to gray, irregular to smooth, extremely hard nodule is present in the left cerebral hemisphere, expanding outward from just above the rostral left lateral ventricle and corpus callosum. On cross section, the nodule is smooth, opaque, and white to pale yellow. Adjacent cerebral tissue is compressed, as is the left lateral ventricle.

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Case History:

Case #: NP-14-1040

Presenter: Sarah Coe, R. Mick Fulton, Dodd Sledge

Corresponding Institution: Michigan State University Veterinary Diagnostic Laboratory, Lansing MI

Signalment: Adult female turkey

History:

This turkey had a history of myriad swollen nodules over the top and both sides of the head, eye lids, proximal neck, and snood.

Gross Findings:

The top and both sides of the head, both eyelids, and snood had myriads of white firm raised nodules ranging in size from 0.5 to 1.0cm in diameter. Most nodules had brown to black crusts on the surface. The nodules also affected feather follicles. The crop, near the entrance of the esophagus had a raised round area that was approximately 4.0x3.0x1.0cm and had multiple red foci on the mucosa.

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Case History:

Case #: 19-35867-2

Presenter: Natalie Kirk, DVM

Corresponding Institution: University of Illinois at Urbana-Champaign

Signalment: 10-year-old, male castrated domestic longhair cat

History: This cat presented to the referring veterinarian for pruritis and patches of erythema with miliary dermatitis on the neck. He did not respond to antibiotics and anti-inflammatory doses of steroids. His lesions increased in size and severity and progressed to involve the skin surrounding the base of his nails and the perianal region.

Gross findings: None

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Case History:

Case #: A19-17017

Presenter: Melissa Swan

Corresponding Institution: Purdue University Department of Comparative Pathobiology

Signalment: Twenty month old spayed female domestic shorthair

History: The patient had a 10 day history of weakness, lethargy, and hyporexia which progressed to ataxia and vision loss.

Gross findings: On gross autopsy the lateral ventricles were dilated and bordered by abundant bulging white matter.

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Case History:

Case #: Burcham-01

Presenter: Grant N. Burcham

Corresponding Institution: Heeke Animal Disease Diagnostic Laboratory and Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University

Signalment: White-tailed deer, buck

History: This adult male White-tailed deer was legally harvested by a hunter on November 24, 2018 within the Hoosier National Forest, at a property known as Paw paw marsh. The deer was markedly emaciated and the entire carcass was submitted for autopsy.

Gross findings:

The buck was markedly emaciated and dehydrated. The left carpus was surrounded by fibrous connective tissue and the joint space was filled with purulent exudate.

The liver was diffusely white to tan, with multiple firm areas, as well as numerous, variably-sized masses bulging from the capsular surface. The masses corresponded with abscesses which were throughout the parenchyma. Numerous black foci, including serpentine tracts, were throughout the hepatic parenchyma. Four large (5 cm in length), red to tan flukes, as well as numerous, small (5-10 mm) flukes were throughout the hepatic parenchyma. Thickened areas of fibrosis surrounded the flukes and formed channels throughout the liver. Hepatic parenchyma, where recognizable, was diffusely friable and tan.

Numerous acorns and partially digested forage were in the rumen; formed feces were in the colon.

The nasaopharynx contained fly larvae (bots).

A few thin, filarial-type nematodes were free within the thoracic cavity. The right caudal lung lobe contained a small (5 to 10 mm), clear cyst with a single, short, elongated white structure (suspected tapeworm larva) inside. The cyst appeared to be on the pleural surface near or adjacent to the diaphragm.

Gunshot related lesions: The proximal portion of the left humerus was fractured into multiple fragments with little to no surrounding hemorrhage. The 4th rib on both the left and right side of the thorax was fractured at the junction with the sternum. A 3.5 cm hole was in the skin at the fracture site on the left side. The left cranial lung lobe was red to purple. The pericardial sac and right ventricle were torn. The heart was surrounded by clotted blood.

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Case History:

Case #: 17-H46849-12

Presenter: Andrea E. Pohly, DVM


Signalment: 13-year-old, spayed female, Old English Sheepdog

History: This animal presented to the ER for acute vomiting. Abdominal ultrasound showed multiple nodules within the spleen, a thickened stomach wall and a potential lymph node which was enlarged next to the stomach. Fine needle aspirate of the stomach wall was consistent with eosinophilic inflammation while the splenic nodule was consistent with lymphoid hyperplasia. Given this information, the primary differentials were an allergic gastritis or neoplasia; full-thickness gastrointestinal biopsies were recommended to further characterize the changes in the stomach. Biopsies were taken of the liver, splenic lymph node, and gastrointestinal tract. A splenectomy was also performed at this time.

Gross findings: On exploratory laparotomy, a small nodule on the left lateral liver lobe, an enlarged splenic lymph node, and mild thickening of the antrum of the stomach were identified.

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Case History: Case #: NC-19-575 Presenter: Leah Stein, Annie Zimmerman, Kurt Williams, Sarah Corner Corresponding Institution: Michigan State University Veterinary Diagnostic Laboratory Signalment: 13-year-old, castrated male Ocicat History:

This Ocicat was diagnosed with nasal lymphoma in January 2019 and was being treated with chemotherapy and radiation therapy. In mid-April he developed severe respiratory distress and presented to Michigan State University Veterinary Medical Center with severe dyspnea, open mouth breathing, and hypoxemia. CT scan was suggestive of pneumonia. A left cranial lung lobectomy was submitted to the Michigan State University Veterinary Diagnostic Laboratory (VDL) and histopathologic findings were consistent with chronic constrictive airway disease, but nonspecific as to the underlying cause. Respiratory cultures were negative. The cat remained severely hypoxemic despite hospitalization and treatment with antibiotics, steroids, and oxygen supplementation. Due to his poor quality of life, euthanasia was elected and he was submitted to the VDL for post mortem examination.

Gross findings: The overall body condition score was 2/9, as there were low amounts of subcutaneous and visceral fat, and there was marked muscle atrophy of the hind limbs and hips. Nasal turbinates were multifocally dark red. The thoracic cavity contained approximately 25 ml of red-tinged, clear, watery fluid, and there were few thin, fibrinous adhesions between the costal pleura and ventral lungs. The remaining lung lobes were diffusely wet and contained patchy areas of collapse that were red to plum, regions that were tan and somewhat firm, and regions that were pink and somewhat spongy.

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Case History:

Case #: MSU VDL 4217

Presenter: Annie Zimmerman, Kurt Williams, Dodd Sledge


Signalment: 6 mo female intact NOG mouse

History:

This experimental cohort was housed in sterile caging on corncob bedding, and provided RO water and standard rodent chow. These mice received five injections of DAUDI cells suspended in PBS buffer. 21 days post-injection, multiple mice were found dead. Remaining mice were hunched, ruffled, squinting, pale, thin, and dehydrated; affected mice continued to die despite supportive care.

Gross findings: There were low amounts of subcutaneous and visceral fat, and the animal had a body condition score of 2/9. The animal was hunched, the fur was unkempt, and the fur over the scapulae was stained yellow. There were dozens of 1mm – 2mm in diameter tan, smooth, slightly raised nodules randomly scattered along the diaphragmatic surface of all liver lobes; on cut section, the nodules were homogenous and extended into the underlying parenchyma. There was a similar 2mm in diameter nodule at the cranioventral aspect of the body of the spleen. The renal capsular surfaces were largely obscured by dozens of similar 1mm – 2mm in diameter, multifocal to coalescing nodules that extended into the underlying parenchyma. The left ovary was 6mm x 5mm x 2mm and the right ovary was 8mm x 5mm x 3mm. No other significant lesions were observed.

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Case History:

Case #:

Presenter: Eileen Henderson1,2, Ryan Colburn3, Rebecca Smedley1

Corresponding Institution: 1Michigan State University Veterinary Diagnostic Laboratory, 2Michigan State University – Aquatic Animal Health Laboratory, 3John Ball Zoo

Signalment: Bluegill, age and sex unknown

History: This bluegill is reported to have multiple cutaneous nodules that were subsequently surgically removed. One nodule was submitted for histopathology. Gross findings:

Multiple 1-3 mm diameter raised, white to tan, exophytic cutaneous masses were present on the fins of this fish. Other fish exhibited similar lesions.

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Case History:

Case #: 190422019

Presenter: Priscila Serpa


Signalment: Feline, 11-year-old, female, spayed, Maine Coon

History: A pink cyst on the lateral aspect of the left tarsus was found during an annual health check examination. The cat is otherwise healthy.

Specimen: Fluid from nodule on tarsus, cytocentrifuge preparation and sediment smear, Modified Wright stain (digital slide).

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Case History

Presenter: George Sandusky

Signalment: 75-year-old Male, 5’11”, and 70 kilograms

Clinical History and Medications:

75-year-old man presents with numbness in the feet. States the numbness started several weeks ago but recently became too uncomfortable. The patient did not complain of fever, pain, malaise, weakness, or urinary tract symptoms.

Physical exam was normal

Physician ordered urinalysis and labs – found to be normal

Patient returned three weeks with same issue

Had abdominal radiographs and MRI

Scan found mass on kidney, extension of the tumor into the renal vein, vena cava, and right atrium of the heart.

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Case History:

Case #: A19-1484

Presenter: Caitlin E. Brown, DVM and Yava L. Jones-Hall, DVM, Ph.D., DACVP


Signalment: 10-week-old, male neutered, domestic shorthair cat

History: A 10-week-old, male neutered, domestic shorthair cat presented to the referring veterinarian on July 26, 2018 after low-cost neuter, for paraparesis, left hind limb swelling, a small wound over the left stifle, and fever. An antibiotic injection (Convenia®) was administered. The kitten presented again on July 31, 2018 for severe weakness and dehydration. Swelling of the right stifle and bilateral carpal joints, along with progression of the left stifle wound and hypothermia (96.7 ºF) were noted. Due to the poor prognosis, the kitten was euthanized. Two other littermates had similar clinical signs and a third litter mate died acutely. All three kittens were being fostered at the time of presentation. Gross findings: Submitted for autopsy was a reportedly 10-week-old, 600g, yellow and white, male neutered domestic shorthair. The cat was in poor body condition with minimal subcutaneous and intraabdominal adipose tissue. There was a 1cm x 0.7cm defect in the skin overlying the medial aspect of the left hock. The wound was open down to the level of the bone, which was visible. This defect was associated with an open comminuted fracture of the left distal tibia. Bone fragments at the fracture site were rough and encased in thick, yellow, gelatinous, friable material. Yellow, cloudy, thick fluid extended from the fracture site proximally to the level of the stifle, dissecting between muscle and fascia. The left popliteal lymph node was markedly enlarged. An abscess was present over the right stifle measuring 1.5cm x 2.5cm x 1cm. Underlying this abscess there was a 2mm x 2mm dissecting defect of the proximal cranial tibia that extended 7mm into the proximal epiphysis. A thick cap of yellow, gelatinous, friable material was loosely adhered to the epiphyseal defect.

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Case History: An experimental model of lung cancer brain metastases was developed using 6-week-old Hsd:Athymic Nude-Foxn1nu mice . Within four to six weeks of cellular inoculation, 14/36 mice developed hind limb paralysis characterized by rigidly extended hind limbs, absence of pinched toe reflex, and tail dragging.

Case #: CBBBL-23

Presenter: L. Tiffany Lyle, DVM, PhD, DACVP

Corresponding Institution: Purdue University College of Veterinary Medicine

Signalment: 10-week old, Female, Hsd:Athymic Nude-Foxn1nu mouse; 18 g

Gross findings: The peritoneum of the cranial abdomen contained numerous, 0.5-1 mm diameter, pale tan, round, firm nodules arranged in a cobblestone-like pattern. A single ovary was enlarged, pale-tan, and measured 6 mm in diameter. On cut section the ovary was polycystic, pale, tan, and irregular. Gross lesions were not identified in other parenchymal organs, vertebrae, spinal cord, or brain.

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Case History: Case #: 7980018 Presenter: Sara Obeiter, DVM Corresponding Institution: University of Missouri Veterinary Medical Diagnostic Laboratory Signalment:

12 year old male castrated Pointer dog

History:

The dog was initially presented to the University of Missouri Veterinary Health Center (MU-VHC) oncology service on August 21, 2018 for evaluation of previously diagnosed chronic lymphocytic leukemia. In October 2017, the dog was incidentally found to have a peripheral lymphocytosis on routine in-house bloodwork before a dental at the rDVM (IDEXX ProCyte, 35.24 K/µL), and was diagnosed with chronic lymphocytic leukemia. In-house bloodwork performed two weeks after the initial bloodwork in October 2017, and again in December 2017, showed consistently elevated lymphocytes (31.04 K/µL and 32.38 K/µL, respectively); no CBC morphology comments were noted in the history and it is unknown if a blood film was evaluated. Treatment with prednisone was started in October 2017 and the dog did well clinically, however the prednisone was discontinued a few weeks before the dog was presented to MU-VHC because the owner thought he was having difficulty breathing while on the prednisone. A CBC performed at MU-VHC on 8/21/18 revealed mild anemia (35%) and lymphocytosis (28.98 K/µL). The majority of lymphocytes were granular lymphocytes with ovoid to slightly reniform eccentrically located nuclei measuring approximately 1.25-2x the diameter of a RBC (Figure 1). Nuclei contained loosely clumped chromatin. These lymphocytes contained moderate amounts of pale blue cytoplasm and few coarse to fine pink cytoplasmic granules. The findings were most consistent with chronic lymphocytic leukemia of granular lymphocyte origin.

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Figure 1. Granular lymphocytes present in the blood collected on 8/21/18 (100x objective).

The dog returned to MU-VHC oncology service on 9/25/18 for a recheck appointment. The dog had generally been doing well at home with a good appetite and energy level, however, several days before the appointment he had a few episodes of vomiting and seemed lethargic. Additionally, he had developed progressive lymphadenopathy and dorsal strabismus. When the vomiting and lethargy developed, he was brought to his rDVM, where a steroid injection was given (dose unknown). Bloodwork performed at the visit to MU-VHC on 9/25/18 was largely unchanged from the visit on 8/21/18, and revealed a low hematocrit of 32% and lymphocytosis of 29.58 K/µL. The dog was re-started on prednisone for palliative care.

On 10/9/18, the dog visited the rDVM where he was administered 6 mg (0.23 mg/kg) of dexamethasone and prescribed cerenia; improvement in the dog’s appetite was noted after this visit. The third and final follow up visit at MU-VHC occurred on 10/10/18. The dog’s lymph nodes were still markedly enlarged, and bloodwork was performed. A blood smear from this visit is included in your slide set.

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Case History

Case #:

AC19-050

Presenter:

Wayne Buck


AbbVie 1N Waukegan Rd North Chicago, IL 60064

Signalment:

1 yr, female beagle dog

History:

1 year old female laboratory beagle dog eliminated from study randomization due to small size and behavioral difficulties. May 31, 2019 elevations in liver enzymes was criterion for elimination from study randomization.

• ALT 234 • AlkP 127 • AST 123 • GGT 6.49

June 5, 2019 observed ataxia, hypersalivation, increased body temperature consistent with recovery from seizure. Treated with midazolam but tremors and convulsion occurred resulting in election for euthanasia.

Gross findings:

Microhepatica with scalloped margins. Marked dilatation and congestion of caudal vena cava and splanchnic veins. The gall bladder was enlarged. The submitted slide was from an area of the liver that was abnormal in shape and contour and close to the gall bladder. A vascular anomaly was not detected at necropsy.

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Case History: Case #: 7679

Presenter: Kerry Goldin, Mayra Tsoi, Victoria Lyons, Dodd Sledge


Signalment: 3.5-year-old castrate male Siberian Husky

History: This dog had a 1-month history of decreased vision, increased intraocular pressure OU, and uveitis OU that was well controlled OS, but uncontrolled OD. There was also iris bombe OD, buphthalmia OD, PIFM OU, eye color change from blue to yellow, diffuse corneal edema OD, fibrin on the endothelium OD. While there was no view of the fundus OD, OS had a mottled fundus and depigmented lesions. The intra ocular pressure was 20 mmHg OD and 26 mmHg OS.

Gross findings: The globe is enlarged, and the sclera appears diffusely thin. The iris is mottled yellow, and the entire uvea is unpigmented to poorly pigmented. There is peripheral neovascularization and mild edema in the cornea. On sagittal section, portions of the retina are segmentally detached, and there is liquefaction of the vitreous.

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Case History:

Case #: S19-1808

Presenter: Adrianne Glaser, Gregory Hoagland, DVM, Abigail Cox, DVM, PhD, Dipl ACVP, Grant N. Burcham, DVM, PhD, Dipl ACVP, Tsang-Long Lin, DVM, PhD


Signalment: 4.5-week-old, male and female; broiler chicks

History: 5 live and 6 dead, 4.5-week-old broiler chicks were submitted to the Indiana Animal Disease Diagnostic Laboratory with a history of head turning and increased mortality.

Gross findings: Submitted for necropsy were 5 live and 6 dead 4.5-week-old broiler chicks weighing 67-119 g. Empty crops were found on 5/5 birds. One bird showed white foci throughout the duodenum. Rubbery bones and beaks were seen on 5/5 birds. 5/5 birds had flared costochondral junctions of ribs and enlarged ribs at the junction with the spine.

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Case History:

Case #: A19-11134

Presenter: Jess Hanlon


Signalment: 8-year-old, neutered male, black and rust Doberman Pinscher

History:

In December of 2018, this dog had his left hind limb amputated due to the presence of osteosarcoma in this limb. He was part of a clinical trial in which he received EC-17 and a folate scan prior to the amputation of the limb. He also received one dose of carboplatin. At the time of amputation, there was no evidence of metastasis of the hind limb osteosarcoma.

In February 2019, this dog presented to the Purdue University Veterinary Teaching Hospital Emergency Department for a recent history of two episodes of profound lethargy and hypothermia. Hemoabdomen was confirmed. He was euthanized and submitted to the Indiana Animal Disease Diagnostic Laboratory for necropsy.

Gross findings:

The abdomen contains approximately 0.5 L of clotted and unclotted blood. The head of the spleen has a ruptured, approximately 10 X 11 X 4 cm, friable, dark red mass consisting of an outer capsule partially surrounding an accumulation of clotted blood. The omentum is partially adhered to the mass. An approximately 0.6 X 0.6 cm, firm, pale tan, raised mass is within the splenic parenchyma near the tail of the spleen.

An approximately 1.8 X 2.4 X 1.4 cm, firm, red, raised nodule is within the parenchyma of the left medial liver lobe. Multifocal, 0.1 X 0.1 cm to 0.4 X 0.4 cm, hard, pale tan nodules are within all lung lobes.

The left hind limb is absent due to a previous amputation (history reported osteosarcoma in the left hind limb and subsequent amputation of that limb).

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Case History:

Case #: 19RD1977

Presenter: Gillian C. Shaw, DVM, PhD, DACVP

Corresponding Institution: University of Wisconsin, Comparative Ocular Pathology Laboratory of Wisconsin

Signalment: 4-year-old spayed female cat of unspecified breed

History: Two month history of uveitis. Began in right eye, with signs developing in left eye several weeks later. Previously managed with topical prednisolone, flurbiprofen, ofloxacin, dorzolamide and tacrolimus, but uveitis and secondary glaucoma have failed to respond. Presented for second opinion. Both eyes were buphthalmic. The right eye had severe corneal vascularization and a yellow opacity in the anterior chamber. The left eye had severe aqueous flare and posterior synechiae. Patient had normal bloodwork and was negative for both FeLV and FIV. Seropositive for both feline coronavirus and histoplasma. She had a very mild pulmonary interstitial pattern on thoracic radiographs, lymphadenopathy of medial iliac and mesenteric lymph nodes (but still too small to sample). Both eyes were enucleated and submitted to COPLOW.

Gross findings: Both eyes were buphthalmic with expanded and tan irises and ciliary bodies. The right eye had copious tan flocculent exudate in the anterior chamber with smaller amounts of exudate on the surface of the ciliary body and retina with a little bit of intraocular hemorrhage. The lens was also opaque (cataract) in the right eye. Slides of the right eye were submitted for MAVP.

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Case History:

Case #: 24460

Presenter: Catherine Shoemake

Corresponding Institution: University of Missouri Veterinary Medical Diagnostic Laboratory

Signalment: 3.5-year-old female spayed standard poodle

History: Five months prior to presentation, the patient presented to her primary veterinarian for evaluation of recent development of regurgitation. The patient was initially thought to have myasthenia gravis based upon initial radiographic findings; however, acetylcholine antibody receptor titers were within normal limits. A left-sided neck mass was then removed six weeks prior to presentation. Following this procedure, the patient developed progressively worsening left forelimb lameness. Two to three weeks prior to presentation a CT scan performed at another hospital revealed a mass on the left aspect of the neck that extended from the caudal mandible into the cranial mediastinum. Partial surgical resection was performed. Following recovery, the patient had increasing difficulty eating and swallowing. Physical exam results upon presentation to the University of Missouri Veterinary Health Center revealed significant muscle wasting (BCS 2/9), non-weight bearing lameness of the left forelimb, left-sided Horner’s syndrome, conscious proprioceptive deficit of the left forelimb with neurologic lesion localization to C6-T2 and brachial plexus, a decreased swallowing reflex, and numerous moderately to markedly enlarged lymph nodes ranging from the mandibular region caudally along the left side of the neck and into the cranial mediastinal space. No overt abnormalities were detected on CBC, serum chemistry, and evaluation of total T4 and TSH. A CT scan revealed a large cranial mediastinal mass consistent with previous reports. Fine needle aspirates of the cranial mediastinal mass and left prescapular lymph node were collected for cytologic analysis. Histology slides submitted are prepared from samples collected at the time of partial surgical resection of the mass. Representative images of cytologic findings are provided below.

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Figure 1. Aspirate of a cranial mediastinal mass from a 3.5-year-old female spayed standard poodle. Wright-Giemsa stain. 600X magnification.

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Figure 2. Aspirate of a cranial mediastinal mass from a 3.5-year-old female spayed standard poodle. Wright-Giemsa stain. 1000X magnification.

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Figure 3. Aspirate of an enlarged left prescapular lymph node from a 3.5-year-old female spayed standard poodle. Wright-Giemsa stain. 600X magnification.

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Case History: Case #: 19-H42694-3 Presenter: Miranda D. Vieson, DVM, PhD, DACVP and Sarah Gray, DVM Corresponding Institution: University of Illinois Veterinary Diagnostic Laboratory and University of Illinois Equine Medicine and Surgery Service Signalment: 8-year-old, Paint Horse gelding History: Presentation to UIUC Equine Medicine and Surgery as an emergency for colic and recently decreased water consumption, feed consumption, and production of feces. Bloodwork showed mild electrolyte abnormalities and slight dehydration (PCV 42%, TP 6.8 g/dL, lactate 1.4 g/dL). Rectal palpation revealed impaction of the pelvic flexure. No nasogastric reflux obtained after tube passage. Exploratory surgery revealed a focal thickening of the pelvic flexure of the colon. Approximately 80 percent of the colon was resected. The abnormal thickening was submitted to UIUC Veterinary Diagnostic Laboratory for histopathology. Gross Findings (per surgery report): At the pelvic flexure, there is a focal, erythematous, mural thickening measuring approximately 6 x 6 cm. The wall was thickened to approximately 3 cm with intramural hemorrhage and fibrous plaques.

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Case History:

Case #: SP-19-7690

Presenter: Glenn Brado, Leah Stein, Sarah Corner


Signalment: 20-year-old, Thoroughbred Gelding

History:

This horse had intermittent unilateral (left-sided) epistaxis that was previously associated with exercise but has recently increased in frequency with no apparent inciting cause. Skull radiographs showed a soft tissue opacity in the left frontal sinus. A left frontal sinusotomy was performed and the mass was removed and submitted to the Michigan State University Veterinary Diagnostic Laboratory (MSU VDL).

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Case History:

Case #: 19-07742-1

Presenter: Rosalie Ierardi, DVM

Corresponding Institution: University of Missouri Veterinary Medical Diagnostic Laboratory

Signalment: 2 year old castrated male Labrador Retriever

History: This dog was presented to the referring veterinarian on April 1 for evaluation of “swollen eyes”. The dog was prescribed an ophthalmic ointment and discharged. During the next week, the dog re-presented to the referring veterinarian twice for inappetence, ataxia, and seizures. The dog was hospitalized twice and treated with a variety of medications, including several doses of dexamethasone. On the morning of April 9, the dog was sternally recumbent with clinical and radiographic signs of pneumonia. The dog had multiple seizures, and the owners elected euthanasia. The body was submitted to the MU VMDL for postmortem examination.

Gross findings: The dog was inadequate nutritional condition. Both tonsils were mildly enlarged. Multifocal to coalescing areas in all lung lobes were dark red, firm, and sank in formalin. Approximately 75% of the total lung tissue was affected. No other gross abnormalities were apparent.

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Case History:

Case #:

SP-19-4881-1

Presenter: Ryan Yanez, Annie Zimmerman, Gwen Sila, Dodd Sledge


Michigan State University Veterinary Diagnostic Laboratory

Signalment:

11 year-old, male castrated, domestic shorthair cat

History:

On initial examination, the referring veterinarian identified severe conjunctivitis OD, corneal edema OD, and green discharge OD that persisted despite 1 month of medical treatment for suspected feline herpesviral (FHV-1) infection. Examination by a veterinary ophthalmologist revealed periocular crusting, discharge, third eyelid elevation, severe corneal granulation tissue, and a central fibrin clot with pigment OD. The right eye was nonvisual with no dazzle, direct or indirect PLR. Normal dazzle and direct PLR were seen OS. OD was fluorescein positive and had an IOP of 13 mmHg. OS was fluorescein negative and had an IOP of 17 mmHg. Corneal cytology of the granulation tissue was performed and identified numerous epithelial cells, abundant neutrophils, and a single eosinophil.

Gross findings: On gross examination of the formalin-fixed globe, the cornea is diffusely opaque, tan, and thickened with an irregular surface. There is a sharply-demarcated, tan to dark brown, deep axial corneal depression. On cut section, the thickened cornea expands into and obliterates the anterior chamber. The iris and ciliary body stroma are moderately thickened by similar pale tan, irregular material.

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Case History

Case #: 19-0501

Presenter: Kimberley Sebastian

Corresponding Institution: Michigan State University

Signalment: 1.5-year-old female spayed German shepherd dog

History: The patient was presented to the Michigan State Veterinary Medical Center (MSU-VMC) for evaluation of progressive paraparesis and inappropriate urination of three weeks duration. On physical examination, the dog was bright and alert with non-ambulatory paraparesis and absent pelvic limb proprioception. Pain was elicited upon bilateral hip extension and rectal examination. There were normal myotatic and perineal reflexes, and an inconsistent bilateral cutaneous trunci reflex. Focal spinal hyperesthesia was exhibited at the level of the lumbar-sacral junction and upon lifting the tail.

A complete blood count showed mild leukocytosis (16.4 𝗑𝗑 103 /µL; RI 4.6-10.7) characterized by a mild neutrophilia (12.2 𝗑𝗑 103 /µL; RI 2.6-7.5) and moderate monocytosis (2.0 𝗑𝗑 103 /µL; RI 0.2-0.5). Serum chemistry revealed mild hyperproteinemia (7.6 g/dL; RI 5.4-6.7) with mild hypoalbuminemia (2.5 g/dL; RI 2.8-3.6) and mild to moderate hyperglobulinemia (5.1 g/dL; RI 2.3-3.7), mild hyperphosphatemia (6.5 mg/dL; RI 2.7-5.4), mild hyperglycemia (120 mg/dL; RI 81-118) and mild elevations in AST (59 U/L; RI 16-41) and CK (177 U/L; RI 51-169) enzyme activities. Urine was yellow and mildly turbid with a specific gravity of 1.025 (refractometric), pH 6.1 (pH meter), 2+ out of 4+ protein, and moderate heme on urine test strip. Sediment examination showed 2-4 RBC/ hpf.

A magnetic resonance image (MRI) revealed multifocal lymphadenopathy (axillary, cranial mediastinal, sternal, tracheobronchial, splenic, sublumbar and peri-aortic), splenomegaly and discospondylitis (T1-T2, T10-T11) characterized by compressive empyema, neuritis and myelitis.

Gross findings: The cranial mediastinal and sublumbar lymph nodes were tan, markedly enlarged at 2-4 cm in diameter, had an irregular surface dissected by mild hemorrhage, and on cut surface had a diffusely granular texture. The spleen was mildly diffusely enlarged, congested, and contained several well-demarcated, smooth, dark red nodules up to 5 cm in diameter. On cut section, these regions contained gelatinous hemorrhage and few peripheral scattered 1-2 mm in diameter tan foci.

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Case History:

Case #: SP-19-7167

Presenter:

Steven Hsu1,2, Rebecca Smedley1

Corresponding Institution: 1Michigan State University Veterinary Diagnostic Laboratory 2National Institutes of Health, Comparative Biomedical Scientist Training Program

Signalment:

9-year-old, male castrated, golden retriever

History:

The dog was transferred to the MSU Neurology Service for evaluation of progressive hind limb lameness. The lameness began suddenly and got progressively worse. Clinical signs did not improve with prednisolone and gabapentin. At the second visit (44 days later), the patient was showing signs of neurologic progression evidenced by mild conscious proprioceptive deficits. MRI revealed an aggressive L2 vertebral body mass that extended along the left lamina and pedicle and caused severe extradural compression of the spinal cord. At surgery, cytology of the mass was consistent with a plasma cell neoplasm, and splenic cytology was consistent with plasmacytosis. Pre- and post-chemistry panel and urinalysis did not reveal hyperglobulinemia or Bence-Jones proteinuria. Post-operation CT revealed an additional lytic lesion located at the dorsal lamina of L4.

Gross findings: Not applicable.

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Case History:

Case #: SP-18-3695

Presenter: Allison Gerras, Sarah Coe, Wallaya Manatchaiworakul, Annie Zimmerman, Dodd Sledge

Corresponding Institution: Michigan State University Veterinary Diagnostic Laboratory, Lansing, MI

Signalment: 15-year-old, spayed female, mixed breed dog

History:

This dog had a 1-month history of exophthalmos OS, normal intraocular pressure, and no loss of vision. A CT of the head revealed a contrast enhancing mass in the left retrobulbar space with no involvement of the orbital bone.

Gross findings: On gross examination of the formalin fixed globe, there was well-circumscribed mass expanding the dorsomedial periocular soft tissues. On cut section, the mass was mottled light to dark brown, irregular, and moderately compressed the adjacent superior-posterior globe.

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Case History

Case #: 1170

Presenter: Yue (Polly) Chen, Annie Zimmerman, Paulo Vilar Saavedra, Dodd Sledge


Signalment: 11-year-old, intact male Shilo Shepherd

History:

This dog presented with a one-month history of right-sided epistaxis, frequent face rubbing, and decreased airflow through the nasal cavity. Physical exam revealed a soft tissue swelling on the right lateral aspect of the muzzle, and airflow was decreased through the left naris and absent through the right naris. Computed tomography (CT) identified a destructive nasal lesion that occupied the entire right nasal cavity and most of the left nasal cavity. Histopathology was consistent with a ganglioneuroma. The mass was unresponsive to treatment with intensity modulated radiation therapy (IMRT) based on subsequent CT scans performed 1, 3, and 6 months post-treatment.

Gross findings: On gross examination, the nasal cavities contained a 15 cm x 5.5 cm x 5 cm, expansile, pink, and fleshy-to-gelatinous mass that expanded from the rostral nasal passage, caused lateral deviation of the distal nasal septum, and effaced the distal nasal septum and turbinates. The mass focally eroded and erupted through the right maxillary bone. On cut section, the mass contained scant hemorrhage that surrounded multifocal to coalescing regions of central necrosis. Brown, opaque, viscous fluid filled the frontal sinuses.

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Case History: Case #: NC-19-0000711 Presenter: Anna Gates, Leah Stein, Jennifer Thomas, Bonnie Harrington Corresponding Institution: Michigan State University Veterinary Diagnostic Laboratory Signalment: 3-year-old, spayed female, domestic short hair cat History: This cat presented to the Michigan State University Veterinary Medical Center for anorexia, lethargy, and weight loss. She had a chronic history of allergic dermatitis that was being treated with desensitizing injections and cyclosporine until 2 days prior to presentation. A painful abdominal mass was identified by palpation, and ultrasound showed the mass was consistent with enlarged mesenteric lymph nodes. Aspiration of the nodes revealed numerous macrophages, moderate neutrophils, and large numbers of phagocytized non-staining rod bacteria. A nodular interstitial lung pattern was observed on thoracic radiographs. Despite treatment with antibiotics, the cat developed a fever, marked right forelimb swelling, and was euthanized due to quality of life concerns. She was submitted to the VDL for postmortem examination. Gross findings: A 2.75kg, spayed female cat was necropsied. Autolysis was mild, and there were low to moderate amounts of subcutaneous and visceral fat with an overall body condition score of 4/9. The eyes were moderately sunken into the orbits and mucous membranes and subcutaneous fascia were tacky (dehydration). The subcutis of the right carpus and foot were markedly expanded by clear gelatinous to watery fluid (edema) in the region of the intravenous catheter. The jejunum, ileum, and orad cecum were moderately transmurally thickened by a tan, homogenous infiltrate. The mediastinal, tracheobronchial, and mesenteric lymph nodes were moderately to severely enlarged, ranging in diameter from 5 mm to 4 cm. They were soft, pale tan, and homogenous on cut section. The liver was diffusely mottled red to tan and had an enhanced reticular pattern. The lungs were diffusely rubbery, wet, and mottled dark red to pink (edema and congestion).

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Case History:

Case #: DERM 18-2

Presenter: Ryan Jennings, DVM, PhD, DACVP

Corresponding Institution: The Ohio State University

Signalment: 2.5 year old male-castrated Doberman Pinscher dog

History: 1-2 month history of multifocal, non-pruritic, firm 1-3 cm in diameter cutaneous nodules.

Gross findings: n/a

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Case History:

Case #: MSU-VDL-16-12131

Presenter: Tuddow Thaiwong1, Lauren W. Stranahan2, Derick Whitley3, Fabiano Oliveira4, Matti Kiupel1

Corresponding Institution: 1 Veterinary Diagnostic Laboratory, Michigan State University, College of Veterinary Medicine, Lansing, MI 2 College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 3Antech Diagnostics, Stillwater, OK 4Antech Diagnostics, College Station, TX

Signalment: 11-year-old, Castrated male, West Highland Terrier

History: This dog was reported to have a two-day history of lethargy and vomiting. A mass effect was seen on abdominal radiographs, and a large jejunal mass was observed on abdominal ultrasound.

Gross findings: N/A

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Case History:

Case #: 19-35760-1

Presenter: Janice Pfeiff, DVM


Signalment: 2-month-old, female intact, rat terrier mix breed dog

History: The patient was surrendered to the county humane society after a one week duration of an upper respiratory infection treated with doxycycline. At the time of surrender, the animal was bright, alert, and responsive with mild upper respiratory signs (upper respiratory congestion) and was treated with Clavamox drops BID. Within 48 hours, the animal was improving with no upper respiratory signs, however, over the next two days demonstrated severe deterioration with trembling, dehydration, stiff limbs, and an inability to stand.

Gross findings: The lungs were diffusely mottled pink to dark red and were slightly rubbery upon palpation. All lung specimens floated in formalin.

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Case History:

Case #: SP-19-7347

Presenter: Erica Noland

Corresponding Institution: Michigan State University

Signalment: 3-year-old, male, neutered Collie dog

History: This dog was adopted about 1 year ago. At that time, he had small red dots around his prepuce. The spots grew slowly into raised red lesions. He also started developing sores on other parts of his body. Recently, the sores had been getting much worse. Skin scrape negative. Skin cytology yielded too numerous to count red blood cells, polymorphonuclear cells, and cocci bacteria. Treated for 1 week using Cephalexin and Douxo Chlorhexidine microemulsion. The skin continued to worsen, so biopsy was elected. Five, 4mm skin punches were taken from the axillary (2) and inguinal (3) regions.

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Case History:

Case #: 19-37390-8

Presenter: Patrick Roady, DVM, MS, DACVP

Corresponding Institution: University of Illinois

Signalment: 4-year-old, intact female, crossbred bovine.

History: The initial clinical signs were a head tilt and circling that eventually led to lateral recumbency, opisthotonus and paddling. The right eye was cloudy.

Gross findings: A field necropsy revealed a flabby heart with possible white streaking throughout the myocardium and an extremely enlarged gallbladder. The lungs and brain were unremarkable.

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Case History:

Case #: 18-1196-4

Presenter: Allison Mustonen

Corresponding Institution: The Ohio State University

Signalment: 4 year old neutered male domestic shorthaired cat

History: The patient presented to The Ohio State University College of Veterinary Medicine for a two-month history of severe weight loss, lethargy, anemia, and bilaterally hyperechoic kidneys with loss of corticomedullary distinction as reported by the referring veterinarian. Relevant initial laboratory results included severe non-regenerative normocytic hypochromic anemia, increased BUN, minimally concentrated urine, proteinuria, and an increased urine protein:creatinine ratio. Cytologic evaluation of a bone marrow aspirate revealed a markedly hypocellular marrow with abundant mucinous material. The cat died despite repeated blood transfusions and supportive care.

Gross findings: At autopsy, the skin and mucous membranes were diffusely pale. There was a large amount of coagulated blood in the stomach and the entire length of the gastrointestinal tract. Both kidneys were pale tan and bulged on cross section.

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Case History:

Case #: 725452

Presenter: Mackenzie Long

Corresponding Institution: The Ohio State University, College of Veterinary Medicine, Department of Veterinary Biosciences

Signalment: 27 year old, Haflinger gelding

History: The horse presented to the OSU Large Animal Internal Medicine Service for evaluation of progressive lymphocytosis that was initially identified 7 months earlier. The horse was previously diagnosed with recurrent airway obstruction and pituitary pars intermedia dysfunction, which were being medically managed. Abnormalities on physical examination included increased respiratory rate with increased expiratory effort and lung sounds. Mild non-painful, normothermic pitting ventral edema was also observed.

Gross findings: N/a

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Case History:

Case #: Purdue University 804-471

Presenter: Natalia Strandberg


Signalment: 11 year-old, neutered male papillon

History: The dog was presented to the emergency and critical care department at the Purdue University Veterinary Teaching Hospital (PUVTH) for a one week history of vomiting, diarrhea, melena, hyporexia, and lethargy. The slide is of a blood film one day after initial presentation.

Relevant clinical and clinicopathologic findings: An abdominal ultrasound was performed and revealed bilateral dilation of the renal pelvises, multiple renal cortical cysts, mild perirenal effusion, mild to moderate peritoneal effusion. Additionally, mild prostatomegaly, hepatic nodules, and gallbladder sludge were noted. Evaluation of the peritoneal fluid revealed a transudative effusion (TP: 2.5 g/dL, TNCC: <30,000 cells/μL) Uroperitoneum was ruled out by comparing fluid to serum creatinine and potassium (see below).

Relevant clinicopathologic data on initial presentation* Test Result Flag Ref. Int. Units Total protein (plasma) 6.3 6.0-8.0 g/dL Hct 49.3 37.0-55.0 % WBC (corrected) 2.7 L 6.0-17.0 k/μL Segmented neutrophils 2.2 L 3.0-12.0 k/µL Band neutrophils 0.05 0.00-0.30 k/μL Lymphocytes 0.3 L 1.0-5.0 k/µL Monocytes 0.08 L 0.15-1.35 k/μL Eosinophils 0.05 L 0.10-1.25 k/µL nRBC 127 /100 WBC Platelets 178 L 200-500 k/µL Reticulocytes 293.1 H <100.0 k/μL BUN 25 Creatinine (serum) 1.90 H 0.50-1.50 mg/dL Potassium (serum) 4.7 3.5-5.0 mmol/L Creatinine (urine) 1.9 mg/dL Potassium (urine) 3.8 mmol/L

*Note: the data in the table is from blood collected one day prior to that used for the slide in your set. Therefore the data provided here may be inconsistent with the cytologic findings.

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Case History:

Case #: AC19-125

Presenter: Richard A. Peterson II, DVM, PhD, Diplomate ACVP

Corresponding Institution: AbbVie, Inc.

Signalment: F344 Rat, Female, ~2 years old

History: The rat was part of an aged F344 colony. The rat was euthanized for scheduled histopathology and clinical pathology evaluation. The rat had “cornified” tail lesions which were being treated with topical “woundkote”.

Gross findings: Multiple raised hyperkeratotic lesions on the tail and swelling and ulceration on the left hind foot were seen. Samples of normal skin and skin with lesions from both sites were collected for histopathologic evaluation. There was no gross evidence of LGL leukemia in this animal.

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Case History:

Case #: SP-19

Presenter: Zachary Millman1,2, Mayra Tsoi1, Dodd Sledge1


1. Michigan State University Veterinary Diagnostic Laboratory 2. National Institutes of Health Comparative Biomedical Scientist Training Program

Signalment: 6-year-old, male castrated, Labrador Retriever

History:

This dog’s owner originally noticed a possible intraocular mass OD in mid-February 2019. Examination by the primary veterinarian on 3/26/2019 revealed a mass suspected to be in the anterior chamber OD. The patient was started on one drop of Neo-Poly-Dex q12h OD. The owners did not appreciate any improvement and suspected that the mass was growing. This dog’s vision was not impaired, and the mass did not appear to be causing any discomfort. This dog was otherwise normal. An ophthalmic exam was performed by the referring veterinarian on 4/11/2019 and a mass arising medially from the ciliary body causing dyscoria was identified, in addition to OD 1+/4 conjunctival hyperemia, adhesions from the iris to the mass, a fine cellular deposition at the anterior lens capsule, trace flare with cells, and nuclear sclerosis. OS showed nuclear sclerosis but was otherwise normal. Enucleation was performed on 6/7/2019 and the globe, eyelid, and third eyelid were submitted for histopathologic examination. Gross findings:

On gross examination of the formalin-fixed globe, there was a tan and black mass arising from the region of the iris and ciliary body and expanding into the anterior chamber. The lens nucleus was white and opaque.

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Case History:

Case #: 19N-0191

Presenter: Erika Hoffeld, DVM

Corresponding Institution: University of Wisconsin School of Veterinary Medicine, Madison, WI

Signalment: 12-year-old female spayed mixed breed dog

History: Multiple hepatic masses and a solitary splenic mass were diagnosed via ultrasound. Cytology of an ultrasound-guided fine-needle aspirate was suspicious for sarcoma. The owner elected supportive care only. Four months later, the dog presented again for inappetance, lethargy, and marked weight loss of several weeks’ duration. Euthanasia was elected.

Gross findings: At necropsy, the dog was emaciated (body condition score 1/5). There were 200 mL of serosanguinous peritoneal fluid. The liver was markedly enlarged, comprising 16.9% of the body weight. Throughout all lobes, 85% of the liver parenchyma was replaced by firm, white-tan, multifocal to coalescing, raised, umbilicated nodules, which did not bulge on cut section. These umbilicated regions varied in color from tan to dark red. Throughout the omentum were dozens of discrete, white, firm nodules ranging in diameter from 0.1 cm to 0.4 cm. The head of the spleen was markedly, focally expanded by a light-tan, firm mass measuring 8 x 7 x 6 cm, with multifocal areas of dark red hemorrhage on the surface and on cut section. The adrenal glands were mildly enlarged. The cranial and caudal poles of the right adrenal gland each contained a light tan, firm nodule, measuring 0.4 cm and 0.3 cm in diameter, respectively, which did not bulge on cut section. The capsule of the left and right kidneys each contained over a dozen multifocal firm white masses which sank in formalin, ranging in size from 0.2 to 0.5 cm in diameter. Subtending each capsular mass was a tan indentation on the renal cortical surface, with the tan discoloration extending up to 0.2 cm into the cortex.

Gross appearance of the body cavities, with the liver in the center of the frame

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Case History:

Case #: A19-10264

Presenter: Levi Smith, Stacey Piotrowski, DVM, and Tsang-Long Lin, DVM, PhD


Signalment: 8-year-old male alpaca

History: The animal was found in lateral recumbence with a temperature of 104.7°F. The animal had a 3-month history of weight loss and poor health.

Gross findings: Approximately 2-3 L of amber colored fluid containing fibrin strands was present in the abdominal cavity. Attached to the right side of the larynx were multiple semi-firm pale tan to white masses ranging in size from 2 cm x 2 cm x 1 cm to 6 cm x 6 cm x 7 cm. On cut section, the laryngeal masses had central dark red foci. The liver weighed 3.7 kg with variably sized multifocal to coalescing pale tan to white raised soft nodules that extended into the hepatic parenchyma and effaced approximately 75% of the liver.

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