20x uv 20x visible relative intensity pixels relative intensity field illumination - 20x objective
Post on 21-Dec-2015
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TRANSCRIPT
20x UV
20x Visible
0
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0 100 200 300 400 500 600 700R
elat
ive
Inte
nsity
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0 100 200 300 400 500 600 700
Pixels
Rel
ativ
e In
tens
ity
Field Illumination - 20x Objective
UV Field Illumination
100X Objective (1.4 NA)
12.5% intervals
INTENSITY=230
123
47
20x Vis
10x Vis (2/4/99)
10x UV
20x UV
Good
Poor
Field Illumination
5x 10x 20x 40x 63x
5x 10x 20x 40x 63x
Visible
Ultraviolet
Field Illumination
Axial Resolution - Lens Comparison
40x 63x 100x
610nm390nm
280nm
Axial Distance (m)
FWHMFWHM
FWHM
Axial Resolution Comparison (100X Obj)
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0.0 0.5 1.0 1.5
Relative Axial Distance (m)
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ativ
e In
ten
sity
Full Max Intensity = 245
Half Max = 122.5190 nm
410 nm
Axial Spectral Registration - 63x Obj
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0 0.5 1 1.5 2 2.5 3 3.5 4
Axial Distance (m)
Rel
ativ
e In
ten
sity
647nm488nm568nm
UV - 568 nm Axial Registration - 63x Obj
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0 1 2 3 4 5 6 7 8 9Axial Distance (um)
Rel
ativ
e In
ten
sity
UV
568 nm
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0 5 10 15 20 25Lateral Distance (m)
Rel
ativ
e In
ten
sity
UV
568 nm
Focal Check Beads
Comparison of PMT Values
Inte
nsit
y
Axial Profile
Mirror - Visible Beads - UV/Visible Focal Check Beads
Spectral Registration Check Methods