247b bioe lt supmaterials 011013

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    Lecture Supplemental Materials -Scale of Things, BioMolecular Structures,

    Bonding and Interactions, Water andSolvation, DNA/RNA and Proteins

    Michael J. Heller, PhD

    Departments Nanoeng ineer ing/Bioengineer ing

    Universi ty o f Cal i forn ia San Diego

    La Jolla, CA

    247B Bioelectronics

    January 10, 2013

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    The Scale of Things

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    Supplemental Cells and Biomoleclues

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    Cells and Biomoleclues

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    The Starting BioMolecules and Interactions

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    The Starting BioMolecules and Interactions

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    The Starting BioMolecules and Interactions

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    The Starting BioMolecules and Interactions

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    The Starting BioMolecules and Interactions

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    The Starting BioMolecules and Interactions

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    The Starting BioMolecules and Interactions

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    The Starting BioMolecules and Interactions

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    The Starting BioMolecules and Interactions

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    The Starting BioMolecules and Interactions

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    The Starting BioMolecules and Interactions

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    The Starting BioMolecules and Interactions

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    The Starting BioMolecules and Interactions

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    The Starting BioMolecules and Interactions

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    The Starting BioMolecules and Interactions

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    Basics of DNA - A Key Biological Process DNA

    to RNA to Protein

    Color Atlas of Biochem, Koolman J. et al, Thieme, 1996, pp.195 Mol Bio Cell 4ed pp. 364

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    Basics of DNA - A Key Biological Process

    DNA to RNA to Protein

    Exon

    Intron

    Exon

    Intron

    Hemoglobin Protein

    Structure

    Sequence of Beta-Globin Gene

    -PointMutation!

    Lehninger pp. 196 & 189

    B i f DNA M t ti d G ti

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    Basics of DNAMutations and Genetic

    Diseases

    Certain single base

    mismatches cause seriousdisease Sickle Cell Anemia

    and Thalassemia

    (numerous other genetic

    diseases and cancers)

    Lehninger pp. 220

    H G P j t

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    Human Genome Project

    Science, V291, Feb 16, 2001, pp. 1304-1351

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    Basics of DNA - Where is DNA Located in

    the Cells ?

    Lehninger pp. 30 & 35

    Animal cells5-30 microns

    Plant cells

    10-100 microns

    Nucleus

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    Basics of DNA - Cell - Nucleus - ChromosomesBanding patterns of

    Human chromosomes

    Mol Bio Cell 4ed

    pp. 199 & 202

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    Basics of DNA - Packaging of DNA in

    Chromosomes

    Fluorescent micrograph of cell in mitosis, green Fspindle

    microtubules, red Fcentromeres, blue Fcondensed

    chromosomes. Mol Bio Cell 4ed pp. 556

    Lehn

    ingerpp.36

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    Nucleotides and Nucleic Acids

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    Nucleotides and Nucleic Acids

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    Nucleotides and Nucleic Acids

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    Nucleotides and Nucleic Acids

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    Nucleotides and Nucleic Acids

    Nucleotides and Nucleic Acids

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    Nucleotides and Nucleic Acids

    The corresponding

    figure is on page

    338 of your text

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    Nucleotides and Nucleic Acids

    Ribosomal RNA

    More three dimensional

    type structures

    N l tid d N l i A id

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    Nucleotides and Nucleic Acids

    DNA Hybridization Process

    Denaturation and Annealing

    FIGURE 8-29 DNA hybridization. Two DNA samples to be compared are completely denatured by heating. When the two

    solutions are mixed and slowly cooled, DNA strands of each sample associate with their normal complementary partner

    and anneal to form duplexes. If the two DNAs have significant sequence similarity, they also tend to form partial

    duplexes or hybrids with each other: the greater the sequence similarity between the two DNAs, the greater the number

    of hybrids formed. Hybrid formation can be measured in several ways. One of the DNAs is usually labeled with aradioactive isotope to simplify the measurements.

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    Basics of DNA - A Key Biological Process

    DNA to RNA to Protein

    Mol Bio Cell 4ed pp. 315 Lehninger pp. 908

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    [email protected]

    Office: Atkinson Hall, Rm 2312

    Phone: 858-822-5699

    mailto:[email protected]:[email protected]