247b bioe lt supmaterials 011013

Download 247B BioE Lt SupMaterials 011013

Post on 03-Jun-2018

220 views

Category:

Documents

0 download

Embed Size (px)

TRANSCRIPT

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    1/42

    Lecture Supplemental Materials -Scale of Things, BioMolecular Structures,

    Bonding and Interactions, Water andSolvation, DNA/RNA and Proteins

    Michael J. Heller, PhD

    Departments Nanoeng ineer ing/Bioengineer ing

    Universi ty o f Cal i forn ia San Diego

    La Jolla, CA

    247B Bioelectronics

    January 10, 2013

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    2/42

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    3/42

    The Scale of Things

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    4/42

    Supplemental Cells and Biomoleclues

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    5/42

    Cells and Biomoleclues

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    6/42

    The Starting BioMolecules and Interactions

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    7/42

    The Starting BioMolecules and Interactions

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    8/42

    The Starting BioMolecules and Interactions

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    9/42

    The Starting BioMolecules and Interactions

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    10/42

    The Starting BioMolecules and Interactions

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    11/42

    The Starting BioMolecules and Interactions

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    12/42

    The Starting BioMolecules and Interactions

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    13/42

    The Starting BioMolecules and Interactions

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    14/42

    The Starting BioMolecules and Interactions

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    15/42

    The Starting BioMolecules and Interactions

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    16/42

    The Starting BioMolecules and Interactions

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    17/42

    The Starting BioMolecules and Interactions

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    18/42

    The Starting BioMolecules and Interactions

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    19/42

    The Starting BioMolecules and Interactions

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    20/42

    The Starting BioMolecules and Interactions

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    21/42

    21

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    22/42

    22

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    23/42

    23

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    24/42

    24

    Basics of DNA - A Key Biological Process DNA

    to RNA to Protein

    Color Atlas of Biochem, Koolman J. et al, Thieme, 1996, pp.195 Mol Bio Cell 4ed pp. 364

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    25/42

    25

    Basics of DNA - A Key Biological Process

    DNA to RNA to Protein

    Exon

    Intron

    Exon

    Intron

    Hemoglobin Protein

    Structure

    Sequence of Beta-Globin Gene

    -PointMutation!

    Lehninger pp. 196 & 189

    B i f DNA M t ti d G ti

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    26/42

    26

    Basics of DNAMutations and Genetic

    Diseases

    Certain single base

    mismatches cause seriousdisease Sickle Cell Anemia

    and Thalassemia

    (numerous other genetic

    diseases and cancers)

    Lehninger pp. 220

    H G P j t

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    27/42

    Human Genome Project

    Science, V291, Feb 16, 2001, pp. 1304-1351

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    28/42

    Basics of DNA - Where is DNA Located in

    the Cells ?

    Lehninger pp. 30 & 35

    Animal cells5-30 microns

    Plant cells

    10-100 microns

    Nucleus

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    29/42

    Basics of DNA - Cell - Nucleus - ChromosomesBanding patterns of

    Human chromosomes

    Mol Bio Cell 4ed

    pp. 199 & 202

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    30/42

    Basics of DNA - Packaging of DNA in

    Chromosomes

    Fluorescent micrograph of cell in mitosis, green Fspindle

    microtubules, red Fcentromeres, blue Fcondensed

    chromosomes. Mol Bio Cell 4ed pp. 556

    Lehn

    ingerpp.36

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    31/42

    Nucleotides and Nucleic Acids

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    32/42

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    33/42

    Nucleotides and Nucleic Acids

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    34/42

    Nucleotides and Nucleic Acids

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    35/42

    Nucleotides and Nucleic Acids

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    36/42

    Nucleotides and Nucleic Acids

    Nucleotides and Nucleic Acids

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    37/42

    Nucleotides and Nucleic Acids

    The corresponding

    figure is on page

    338 of your text

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    38/42

    Nucleotides and Nucleic Acids

    Ribosomal RNA

    More three dimensional

    type structures

    N l tid d N l i A id

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    39/42

    39

    Nucleotides and Nucleic Acids

    DNA Hybridization Process

    Denaturation and Annealing

    FIGURE 8-29 DNA hybridization. Two DNA samples to be compared are completely denatured by heating. When the two

    solutions are mixed and slowly cooled, DNA strands of each sample associate with their normal complementary partner

    and anneal to form duplexes. If the two DNAs have significant sequence similarity, they also tend to form partial

    duplexes or hybrids with each other: the greater the sequence similarity between the two DNAs, the greater the number

    of hybrids formed. Hybrid formation can be measured in several ways. One of the DNAs is usually labeled with aradioactive isotope to simplify the measurements.

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    40/42

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    41/42

    Basics of DNA - A Key Biological Process

    DNA to RNA to Protein

    Mol Bio Cell 4ed pp. 315 Lehninger pp. 908

  • 8/12/2019 247B BioE Lt SupMaterials 011013

    42/42

    mheller@.ucsd.edu

    Office: Atkinson Hall, Rm 2312

    Phone: 858-822-5699

    mailto:mheller@.ucsd.edumailto:mheller@.ucsd.edu