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A special conference of the Society for Industrial Microbiology and Biotechnology

Symposium on Biotechnology for Fuels and ChemicalsApril 30 - May 3, 2012Sheraton New OrleansNew Orleans, LAwww.simhq.org/sbfc

34th SBFC

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1 Introductory Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Sponsors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Welcome from the Organizing Committee . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Organizers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Scott Awardee . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Raphael Katzen Awardee . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

General Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Floor Plan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Exhibits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Keynote Speaker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Program at a Glance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2 Technical Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Monday Posters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Tuesday Posters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

3 Abstracts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Paper Abstracts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

Poster Abstracts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

4 Author Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154

Table of Contents

Thank you to our 2012 Symposium sponsors

GenScript

welcome | 3

Welcome from the Organizing CommitteeThe conference organizers welcome you to New Orleans and the 34th Symposium on Biotechnology for Fuels and Chemicals, a special conference of the Society for Industrial Microbiology and Biotechnology (SIMB), hosted this year by the Oak Ridge National Laboratory. We invite you to join us and your colleagues from around the world in this exciting exchange of scientific and technical information and ideas. This year we have included three parallel tracks comprising eighteen oral presentation sessions as well as a special topic evening session and two large poster sessions. The program is designed to highlight the latest research and development accomplishments while providing ample opportunities for technical discussions and networking.

This year’s Symposium is made possible through hard work of the SIMB staff, the session chairs, and the generous financial support of our sponsors, and we urge you to join us in warmly thanking them. This year’s sponsors include:

We also appreciate and acknowledge the participation and support provided by the Tabletop Exhibitors and Program Advertisers. Make sure to visit their exhibits and learn about the latest in research, services, products, and equipment.

A highlight of the annual Symposium is presentation of the Charles D. Scott Award and the Raphael Katzen Award to recognize individuals, or organizations, who have made especially significant technical or commercial contributions to the field of biotechnology for fuels and chemicals.

The Charles D. Scott Award recognizes individuals who have made distinguished contributions to enable and further the use of biotechnology to produce fuels and chemicals. Initiated in 1995, this award is named in honor of Dr. Charles D. Scott, who founded the Symposium on Biotechnology for Fuels and Chemicals and chaired the conference for its first 10 years. This year the Charles D. Scott Award is being presented to Jens Nielsen, Chalmers University of Technology.

The Raphael Katzen Award recognizes individuals or organizations who have made distinguished contributions to enable and further the deployment and commercialization of biotechnology to produce fuels and chemicals from renewable resources. Initiated in 2008, this award is named in honor of Dr. Raphael Katzen, a pioneer in scaling up and commercializing technologies for converting renewable feedstocks into fuels and chemicals. This year the Raphael Katzen Award is being presented to John Ferrell, US Dept. of Energy.

In closing, welcome to New Orleans and to the 34th Symposium on Biotechnology for Fuels and Chemicals! We look forward to interacting with you in another stimulating conference.

AbengoaAdvance Bio LLCBird EngineeringCargillEnergy Biosciences InstituteGenencorGenscriptGevo

IogenKATZEN InternationalNoble FoundationNovozymesU.S. Department of Energy Biomass ProgramVogelbusch

4 | organizers

2012 Program ChairsK. Thomas Klasson, Conference ChairUSDA-Agricultural Research Service, New Orleans, LA

Jonathan Mielenz, Conference ChairOak Ridge National Laboratory, Oak Ridge, TN

Co-chairsSteve Decker, Conference Co-chairNational Renewable Energy Laboratory, Golden, CO

Jim McMillan, Conference Co-ChairNational Renewable Energy Laboratory, Golden, CO

Organizing CommitteeWilliam AdneyResearch Triangle Institute, RTP, NC

Cathy AslesonGevo, Englewood, CO

Bryna Berendzen USDOE Office of the Biomass Program, Golden Field Office, Golden, CO

Doug CameronAlberti Advisors , Minneapolis, MNBrian DavisonOak Ridge National Laboratory, Oak Ridge, TN

Steve Decker, Conference Co-ChairmanNational Renewable Energy Laboratory, Golden, CO

David HogsettMascoma Corporation, Lebanon, NH

Randy JohnsonUS Forest Service, Arlington, VA

K. Thomas Klasson, Conference ChairmanUSDA-Agricultural Research Service, New Orleans, LA

Lee LyndDartmouth College, Hanover, NH

Rubens Maciel FilhoState University of Campinas (UNICAMP), Brazil

Jim McMillan, Conference Co-ChairmanNational Renewable Energy Laboratory, Golden, CO

Jonathan Mielenz, Conference ChairmanOak Ridge National Laboratory, Oak Ridge, TN Lisbeth OlssonChalmers University, Gothenburg, Sweden

Tim RialsUniversity of Tennessee, Knoxville, TN Jack SaddlerUniversity of British Columbia, Vancouver, BC, Canada Jin-Ho SeoSeoul National University, Seoul, Korea Sharon ShoemakerUniversity of California, Davis, CA

Seema SinghJoint BioEnergy Institute/Sandia National Laboratories, Livermore, CA Charles WymanUniversity of California, Riverside, CA

organizers | 5

2012 SIMB OfficersPresidentNeal ConnorsPhoenix BioConsulting LLC, Fanville, NJ

Past PresidentBadal SahaUSDA-ARS-NCAUR, Peoria, IL

President-electTom JeffriesUSDA Forest Products Laboratory, Madison, WI

SecretaryElisabeth D. ElderLouisiana State University-Alexandria, LA

TreasurerJanet WestphelingUniversity of Georgia, Athens GA

SIM DirectorsScott BakerPacific Northwest National Laboratory, Richland WA

E. Timothy DaviesGreen Biologics, Abdington, UK

Erick VandammeGhent University, Gent, Belgium

Russell VreelandUS BARCO, Belle Haven, VA

6 | awards

About the Charles D. Scott AwardInitiated in 1995, the Charles D. Scott Award recognizes individuals who have made distinguished contributions to enable and further the use of biotechnology to produce fuels and chemicals. The award is named in honor of Dr. Charles D. Scott, who founded the Symposium on Biotechnology for Fuels and Chemicals and chaired the conference for its first ten years, A member of the National Academy of Engineering and a past director of the Åmerican Institute of Chemical Engineers Chuck performed research and development on many novel bioprocessing systems while at ORNL including high produc-tivity bioreactors, immoblized microbes, enzymes in organic media, and coal bioprocessing.

Past Awardees of the Charles D. Scott Award1995 – 17th Symposium – Donald J. Johnson1996 – 18th Symposium – Bruce Dale1997 – 19th Symposium – Raphael Katzen1998 – 20th Symposium – Jack Saddler1999 – 21st Symposium – Charles E. Wyman2000 – 22nd Symposium – Karel Grohman2001 – 23rd Symposium – Patrick Foody2002 – 24th Symposium – Sharon Shoemaker2003 – 25th Symposium – Thomas W. Jeffries2004 – 26th Symposium – Guido Zacchi2005 - 27th Symposium – Lee Lynd2006 – 28th Symposium – Brian Davison2006 – 28th Symposium – Mark Finkelstein2007 - 29th Symposium – Lonnie O. Ingram2008 – 30th Symposium - Barbel Hahn-Hagerdal2009 – 31st Symposium - Michael R. Ladisch2010 – 32nd Symposium – Michael E. Himmel2011 - 33rd Symposium – Stephen Picataggio

Jens NielsenJens Nielsen has an MSc degree in Chemical Engineering and a PhD degree (1989) in Biochemical Engineering from the Danish Technical University (DTU), where he established his independent research group and was appointed full Professor in 1998. He was Fulbright visiting professor at MIT 1995-1996. At DTU he founded and directed the Center for Microbial Biotechnology. In 2008 he was recruited as Professor and Director to Chalmers University of Technology, Sweden, where he is currently directing a research group of more than 50 people and the Life Science Area of Advance, which coordinates over 200 researchers from 5 departments. Jens Nielsen has published to date more than 330 research papers that have been cited more than 9,800 times (current H-factor 50), has co-authored more than 40 books, and is inventor of more than 50 patents. He has founded several compa-nies that have raised more than 25 million € in venture capital. He has received numerous Danish and international awards and is a member of the Academy of Technical Sciences in Denmark, the National Academy of Engineering in the United States, the Royal Danish Academy of Science and Letters, the American Instute for Medical and Biological Engineering, the American Academy of Microbi-ology, the Royal Swedish Academy of Engineering Sciences, and the Royal Society of Arts and Sciences in Gothenburg.

2012 Charles Scott Award Winner

awards | 7

About the Raphael Katzen AwardInitiated in 2008, the Raphael Katzen Award recognizes individuals who have made distinguished contributions to enable and further the deployment and commercialization of biotechnology to produce fuels and chemicals from renew-able resources. This award is named in honor of Dr. Raphael Katzen, who was a pioneer in scaling up and commercializing technologies for converting renewable feedstocks into fuels and chemicals. Dr. Katzen was involved in this topic since the 1940s when he first began working on converting wood waste to ethanol. He played a major role in development and improving corn dry milling technologies and in continuing to advance commercialization of lignocellulose conversion technologies.

Past Awardees of the Raphael Katzen Award2008 – 30th Symposium – Raphael Katzen2009 – 31st Symposium – Douglas Cameron2010 – 32nd Symposium – no recipient was selected2011 – 33rd Symposium - Patrick Foody, Sr.

John FerrellThroughout his career, John Ferrell has been actively involved with the advance-ments of feedstock supply systems and energy crop development, recognizing the importance of addressing sustainability issues associated with large-scale biomass production for a biobased industry. After graduating with a bachelor’s degree in biology from Kalamazoo College, he went on to earn his master’s in resource man-agement at Syracuse University and SUNY College of Environmental Science and Forestry. Ferrell has been a key member of the Department of Energy (DOE) Office of the Biomass Program leadership team under Energy Efficiency and Renewable Energy for over 20 years.

During his time at the DOE, he has held several positions including that of designated Federal officer for the Biomass Technical Advisory Committee which oversees programs at DOE, USDA, and other agencies with biomass program activi-ties, and co-chair of the Federal Interagency Working Group for Feedstock Logistics, Distribution and End Use. Ferrell is well known for his work on the development of feedstocks and feedstock logistic technologies, and was a champion for the recently released Billion Ton Study Update, which has already become the founda-tion for all future bioenergy industry assessments and projections and has further established the potential of biomass as a fundamental sector in US Energy Policy. John is the father of two adult sons, and currently resides in Maryland with his wife, Jan. He enjoys spending time outdoors in nature and working in his garden.

2012 Raphael Katzen Award Winner

8 | general information

General InformationAll meeting activities will take place in the Sheraton New Orleans Hotel.

RegistrationOn-site registration, Exhibitor registration and distribution of meeting packets to pre-registrants Napoleon Ballroom Foyer, 3rd fl, during the following hours:

Monday, April 30 8:30 am – 5:00 pm Tuesday, May 1 7:15 am – 5:00 pm Wednesday, May 2 7:15 am – 12:00 pm Thursday, May 3 7:15 am – 3:00 pm

Programs will be distributed at the meeting to all attendees. (Ex-tra copies of the meeting program at the meeting site are $50.)

Name badges must be worn by all attendees, including guests, for admittance to the scientific sessions, exhibits and special functions.

Smoking is not permitted in the hotel.

Special NeedsThe Organizing Committee and SIMB want to ensure your comfort and convenience at the Symposium. If you have any special needs, please let us know at the registration desk.Napoleon Ballroom Foyer, 3rd fl

Internet LoungeAn Internet lounge is available to all attendees during Sympo-sium registration hours.Borgne, 3rd fl

Speaker ready roomSpeakers may review their presentations inPoydras, 3rd fl, during the following hours: Monday, April 30 8:00 am – 9:00 pm Tuesday–Thursday, May 1-3 7:30 am – 9:00 pm

Job FairTuesday, May 1 8:00 am – 4:00 pmNapoleon Ballroom Foyer, 3rd fl

EdeniQ CorpSynthetic Genomics

Placement ServiceSIMB will feature a Placement Service during registration hours. Post your resume or view available positions. Napoleon Ballroom Foyer, 3rd fl

Membership TableThe SIMB Membership Committee would like to welcome new members, first-time attendees and students to the Sympo-sium. Please visit the membership table to learn more about SIMB and its membership benefits. Napoleon Ballroom Foyer, 3rd fl

Information and message centerMessages and announcements will be posted on the bulletin board in the Registration Area. In case of emergency, regis-trants may be contacted through the Sheraton New Orleans Hotel, 500 Canal St., New Orleans, LA 70130; tel. (504) 525-2500

Meals and Social Events

Poster Sessions/ReceptionMonday-Tuesday, April 30-May 1 - 6:00 pm – 9:00 pmNapoleon Ballroom C-D, 3rd fl

Continental Breakfast – all attendeesTuesday, May 1-Thursday, May 3 - 7:15AM – 8:00AMNapoleon Ballroom Foyer, 3rd fl

Invited Speaker Breakfast Breakfast for invited speakers/session chairs on day of presentationMonday, April 30 - 9:00 am – 10:00 amTuesday-Thursday, May 1-3 - 7:15 am – 8:00 amGallier, 4th fl

general information | 9

Lunch – on your own

Banquet ReceptionThursday, May 3Reception 6:00 pmLagniappe, 2nd fl

Banquet and Award PresentationsThursday, May 37:00 pm Armstrong, 8th fl

Hospitality Lagniappe, 2nd flTuesday – Thursday, May 1-3Opens at the close of the evening sessionsSponsored by KATZEN International

10 | floor plan

2nd Floor

floor plan | 11

3rd Floor

12 | exhibitors

TABLETOP EXHIBITSCompany representatives will be available during the Symposium. Please visit the exhibitors in the NAPOLEON BALLROOM FOYER, 3rd fl during the show hours listed:

Monday, April 302:00 pm – 3:00 pm5:30 – 9:00 pm

Tuesday, May 19:30 am – 10:00 am2:30 – 3:00 pm

Wednesday, May 19:30 am – 10:30 am

Andritz Ltd.Tabletop 3Lachine, QC, Canada

Andritz, a global market leader for customized plants, systems and services for various specialized industries, is applying proven pro-cess technology, concepts and machines from the pulp and paper and environmental industry with minor modifications on various types of lingo-cellulosic feedstocks for pre-treatment systems, liquid solid separation or reactor feed systems in the biofuels area.

Applied Instruments Technology (AIT)Tabletop 6Upland, CAAIT manufactures real time process analytical instrumentation including MGATM mass Spectrometer, AnalectTM FTIR, PIONIR near infrared, RPMTM Raman & FXiTM gas chromatographs. Key applica-tions include fermentation off gas analysis and chemical & physical property analysis of biofuels. AIT designs, manufactures and markets these instruments worldwide from its headquarters in Southern California.

Appropriate Technical Resources, Inc. (ATR)Tabletop 14 and 15Laurel, MDATR, the US distributer of Infors AG, will display the new Labfors Lux LED photo bioreactor for the cultivation of photosynthetic organisms and study of biofuel production. The Labfors Lux Flat Panel vessel provides a high quality light source, up to 3000 µmol/m2s (noon in the Caribbean), and simulates a daylight curve. The new touch panel integrated with all Infors bioreactors is OPC/OLE compatible and allows protocols to be expanded from 300 ml glass vessels to 200 L pilot scale. ATR is under license with Infors to manufacturers the Multitron stackable shakers for bacteria, yeast, insect cells, mammalian cells, and algae that can also be fitted with fluorescent or custom LED lighting to research phototrophs and

other organisms.

Applikon Biotechnology, Inc.Tabletop 22 and 23Dover, NJ

For over 30 years, Applikon Biotechnology has provided flexible, reliable fermentation technologies across all scales - from small-scale cultivations and high-throughput screening through process development and production-scale bioprocessing. Our knowl-edgeable team can provide turnkey and custom solutions for your process! Visit us at www.applikonbio.com or call (650) 578-1396 for more information.

Bioengineering, Inc.Tabletop 10Waltham, MA

BioTek Instruments, Inc.Tabletop 11Winooski, VT

BioTek® is a worldwide leader in the design, manufacture and sale of microplate instrumentation including readers, washers, automated pipetting systems, dispensers and software. BioTek instrumentation is used extensively in biofuel research including applications to screen algal strains for lipid production, optimize conditions for lipid production and enzymatic conversion of bio-mass to biofuels.

BlueSens gas sensor GmbHTabletop 17Northbrook, IL

exhibitors | 13

Chemglass Life ScienceTabletop 13Vineland, NJ

Chemglass Life Sciences: Where Chemistry meets Biology. CGLS offers many solutions to the Life Science market including Spinner Flasks, Magnetic Stirrers, Cell Culture Bags, Shakers, Rollers, Rock-ers, Vortexers, Incubators, CellTreat Plasticware, Reusable Glass-ware, Ovens, Refrigerators, Freezers, Polycarbonate Disposable Flasks, Shake Flasks, Fernbach Flasks, Water Baths, Micro Centrifuge Tubes and Vials.

DASGIP BioTools, LLCTabletop 21Shrewsbury, MADASGIP has been an industry-leading supplier of benchtop bio-reactor solutions for the biotech, pharma and chemical industries as well as academia and research institutions since 1991. Our Parallel Bioreactor Systems for the cultivation of microbial, plant, animal and human cells utilize industry-standard benchtop glass and single-use bioreactors. DASGIP’s best-in-class configurable control systems and state-of-the-art bioprocess analyzers deliver unparalleled functionality coupled with user-friendly design for optimal bioprocessing. Our proven solutions for interconnectivity to 3rd party bioprocess analyzers, supervisory control systems and DoE tools, combined with our best-in-class control systems, enable migration strategies for optimization of legacy benchtop bioreac-tor equipment. The outstanding DASGIP bioprocess solutions sup-port QbD-driven process development. In January 2012, DASGIP was acquired by Eppendorf AG, premium manufacturers of liquid, sample and cell handling products and expert partners in life science laboratories. DASGIP’s high quality products complement Eppendorf’s offerings in its customer orientation, performance and reliability.

Energy Biosciences Institute (EBI)Tabletop 16Berkeley, CAThe Energy Biosciences Institute is the world’s largest public/private consortium dedicated to the development of bioenergy. Its part-ners – BP, the global energy company which has committed $500 million to the 10-year program; the University of California, Berkeley; Lawrence Berkeley National Laboratory; and the University of Illinois at Urbana-Champaign – are exploring the application of biological processes, materials and mechanisms to the energy sector.

EppendorfTabletop 20Hauppauge, NY

GenScriptTabletop 24Piscataway NJGenScript is a world leading biology Contract Research Orga-nization backed by the renowned investor KPCB. With tracked performance, we have developed extensive partnerships with pharmaceutical, bio-energy, agricultural, industrial biotech com-panies, to provide time-efficient and cost-effective solutions on gene optimization and synthesis, mutagenesis library, proteins, structural biology, cell line, peptides, antibody, etc.

Metabolon, IncTabletop 5Durham, NC

Metabolon’s biochemical profiling provides early indication of phenotypic changes, guiding and improving strain design/selec-tion, process development and scale up. Pathway tendencies and rate limiting biochemicals can be discovered/monitored, providing comprehensive analysis for optimizing cell physiology, nutrient dynamics, synthetic efficiency and production that is effective in bacterial, yeast, algal and mammalian expression systems.

Molecular Devices, LLCTabletop 2Hampshire, United KingdonMolecular Devices, LLC, offers unrivalled solutions to support basic research, and pharmaceutical and biotherapeutic development, establishing industry standards in picking microbial colonies and screening and selection of mammalian cell lines, as well as provid-ing imaging platforms to monitor cell growth, evaluate cellular responses and quantify protein production.

National Renewable Energy Laboratory (NREL) Tabletop 9Golden, COThrough biomass research, NREL is developing technologies to convert biomass—plant matter such as trees, grasses, agricultural residue, algae, and other biological material—to biofuels. These biofuels will reduce our nation’s dependence on foreign oil, im-prove our air quality, and support rural economies.

14 | exhibitors

optek-Danulat, Inc.Tabletop 12Germantown, WI

optek UV-VIS-NIR and turbidity analyzers provide continuous monitoring of biomass, g/L, optical density, % solids, cell density, dry weight and other feed stock attributes for bio-energy conver-sion and process automation. From aqueous slurries to centrifuga-tion and filtration, optek provides real-time results to characterize and optimize processes for improved control.

Pacific Northwest National Laboratory (PNNL)Tabletop 18Richland, WA

Pacific Northwest National Laboratory (PNNL) delivers solutions to our nation’s biggest challenges in energy, environment, and national security. PNNL provides scientific and technical leadership to meet the growing need for clean, reliable, and secure energy through cutting edge biotechnology, chemical catalysis, carbon management, and techno-economic analysis for conversion of biomass to sustainable infrastructure-ready fuels.

Thermo Fisher ScientificTabletop 4Rockland, IL

Thermo Fisher Scientific provides liquid chromatography and sample extraction instrument solutions to separate, isolate, and identify components of chemical mixtures. The unique chemistry of our columns permits fast separation for compositional analysis of biomass feedstocks, including carbohydrates and lipids. The Thermo Scientific Dionex Chromeleon™ Chromatography Data System (CDS) software provides the tools to quickly turn data into results on virtually any liquid or gas chromatograph.

US Department of Energy Genomic Science ProgramTabletop 19Oak Ridge, TNThe US Department of Energy’s Genomic Science Program (for-merly Genomes to Life) uses microbial and plant genomic data, high-throughput analytical technologies, and modeling and simu-lation to develop a predictive understanding of biological systems behavior relevant to solving energy and environmental challenges including bioenergy production, environmental remediation, and climate stabilization.

keynote speaker | 15

David Glassner, GevoDavid Glassner, Ph.D. has served as Executive Vice President, Technology and Engineering, of Gevo since July 2007, where he leads the company’s isobutanol technology development, engineering and manufacturing. Prior to joining Gevo, he led the development and commercialization of novel yeast biocatalysts for the production of lactic acid and ethanol, and the development and commercializa-tion of lactic acid, lactide and polylactide technology at NatureWorks LLC (for-merly Cargill Dow) from 2000 to 2007. Prior to NatureWorks, he was the Biofuels Technology Manager at the National Renewable Energy Laboratory where he led the development of cellulosic processing technology and the construction of the biomass to ethanol process development unit. Previously, Dr. Glassner was Direc-tor of Bioprocess Development at MBI International, where he led the develop-ment of a lactic acid pilot plant and developed patented processes for producing lactic acid, succinic acid, acetone, ethanol and butanol. Dr. Glassner holds Ph.D., M.S. and B.S. degrees in chemical engineering from Michigan State University.

2012 Keynote Speaker

From Concept to Commercialization – Experiences from Lactic Acid and Isobutanol Many organizations are pursuing the production of industrial biochemicals and fuels using synthetic biology to create ‘magic bugs.’ However, there are very few examples of success in the industrial biochemical and fuels sector because success is driven by highly efficient, low cost and large scale processes which are not easily achieved. This contribution will provide insights on meeting the challenges in moving novel, recombinant fermentation technology from concept to commercialization using lactic acid and isobutanol as examples. The key characteristics of the projects are similar, starting with the definition of a target product and process which together form an economically attractive business opportunity. The business opportunity created by the new product, here assumed to require a new fermentative microorganism, allows the target performance characteristics for the new microorganism to be established from a process economic analysis. Both operating and capital cost for implementation are con-sidered in establishing the microorganism performance targets. However, the performance target is not established by looking at microorganism literature but rather based on a low cost, highly efficient manufacturing process chosen so that the investment made in developing the process will stand the test of time. After using techno economic models to establish the performance tar-gets, resources required to successfully develop the fermentative organism must be assembled. The resources are a combination of funding and technical capabilities. The technical capabilities include personnel, equipment and facilities required to success-fully execute the microorganism development project. Capabilities will make or break a project and certainly play a key role in the time required to reach commercialization or the possibility of ever reaching commercialization. The final component in a success-ful commercialization is leadership. Strong leadership is required to obtain required resources, communicate how, when and why success will be attained and to inspire the team developing the microorganism to success. The key characteristics outlined will be illustrated by a variety of experiences and lessons learned along the road to commercialization of lactic acid and isobutanol producing microorganisms.

16 | program at a glance

PROGRAM AT A GLANCEMonday, April 30

7:30 am – 9:00 pm Internet Lounge Borgne, 3rd fl

8:00 am – 9:00 pm Speaker Ready Poydras, 3rd fl

8:30 am - 5:00 pm Registration Napoleon Ballroom Foyer, 3rd fl

11:00 am - 5:00 pm Poster set up Napoleon Ballroom C-D, 3rd fl

8:30 am - 5:00 pm Placement Service Napoleon Ballroom Foyer, 3rd fl

9:00 am - 10:00 am Invited Speakers’ Breakfast Gallier, 4th fl

12:30 pm - 1:15 pm Opening remarks/Keynote Napoleon Ballroom A-B, 3rd fl

1:00 pm - 4:30 pm Session 1 Napoleon A-B, 3rd fl

1:00 pm - 4:30 pm Session 2 Rhythms, 2nd fl

1:00 pm - 4:30 pm Session 3 Waterbury, 2nd fl

5:30 pm - 9:00 pm Exhibits open/Reception Napoleon Ballroom Foyer, 3rd fl

6:00 pm - 9:00 pm Poster Session I Napoleon Ballroom C-D, 3rd fl

Tuesday, May 1


7:15 am - 8:00 am Continental Breakfast Napoleon Ballroom Foyer, 3rd fl

7:15 am - 8:00 am Invited Speakers’ Breakfast Gallier, 4th fl


7:30am - 9:00 pm Internet Lounge Borgne, 3rd fl

7:30 am - 9:00 pm Speaker Ready Poydras, 3rd fl

8:00 am - 4:00 pm Job Fair Napoleon Ballroom Foyer, 3rd fl

8:00 am – 11:30 am Session 4 Napoleon A-B, 3rd fl

8:00 am – 11:30 am Session 5 Rhythms, 2nd fl

8:00 am – 11:30 am Session 6 Waterbury, 2nd fl

11:30 am – 1:00 pm Lunch on your own

1:00 pm – 4:30 pm Session 7 Waterbury, 2nd fl

1:00 pm – 4:30 pm Session 8 Napoleon Ballroom B, 3rd fl

1:00 pm – 4:30 pm Session 9 Rhythms, 2d fl

5:30 pm – 9:00 pm Exhibits open/Reception Napoleon Ballroom Foyer, 3rd fl

6:00 pm - 9:00 pm Poster Session II Napoleon Ballroom C-D, 3rd fl

program at a glance | 17

Wednesday, May 3

7:15 am - 12:00 noon Registration Napoleon Ballroom Foyer, 3rd fl

7:15 am - 12:00 noon Placement Service Napoleon Ballroom Foyer, 3rd fl


7:15 am - 8:00 am Invited Speakers Breakfast Gallier, 4th fl

7:30 am – 9:00 pm Internet Lounge Borgne, 3rd fl



8:00 am – 11:30 am Session 11 Napoleon Ballroom B, 3rd fl


12:15 pm - 1:45 pm Organizing Committee Lunch Maurepas, 3rd fl

FREE AFTERNOON

7:00 pm - 9:30 pm Special Topic Napoleon A-B, 3rd fl

Thursday, May 3




7:15 am - 8:00 am Invited Speakers Breakfast Gallier, 4th fl

7:30 am – 9:00pm Internet Lounge Borgne, 3rd fl



8:00 am – 11:30 am Session 14 Napoleon Ballroom B, 3rd fl


Lunch on your own

1:00 pm – 4:30 pm Session 16 Waterbury, 2nd fl

1:00 pm – 4:30 pm Session 17 Napoleon Ballroom A-B, 3rd fl

1:00 pm – 4:30 pm Session 18 Rhythms, 2nd fl

6:00 pm – 7:00 pm Banquet Reception Lagniappe, 2nd fl

7:00 pm Banquet Armstrong, 8th fl

18 | technical programm

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Technical Program

Monday, April 30

RegistrationNapoleon Foyer, 3rd fl 8:30 AM-5:00 PM

Invited Speaker Breakfast Maurepas, 3rd fl 9:00 AM-10:00 AM

Exhibit setup Napoleon Foyer, 3rd fl 8:00 AM-2:00 PM

Poster setup Napoleon Ballroom C-D, 3rd fl 11:00 AM-5:00 PM

Opening remarksJonathan Mielenz, ORNL and Thomas Klasson, USDA, 2012 Symposium Chairs

Keynote addressDavid Glassner, Gevo “From concept to commercialization – experiences from lactic acid and isobutanol”

Napoleon Ballroom, 3rd fl 12:00 PM-12:45 PM

SESSION 1: Bacterial Science & Technology I Chair: A. Guss, Oak Ridge National Laboratory, Oak Ridge, TN

Napoleon A-B, 3rd fl

1:00 PM 1-01: Genetic engineering of C. thermocellum for biofuel production R. Biswas and A.M. Guss*, Oak Ridge National Laboratory, Oak Ridge, TN; D. Olson and L.R. Lynd, Dartmouth College, Hanover, NH

1:30 PM 1-02: Understanding the physiology of Thermoanaerobacter thermohydrosulfuricus WC1 through genomic, proteomic and meta-bolic analysis for its usefulness in cellulolytic biofuel producing co-cultures

T.J. Verbeke*, X.L. Zhang, T. Rydzak, V. Spicer, O.V. Krokhin, B. Fristensky, J.A. Wilkins, D.B. Levin and R. Sparling, University of Manitoba, Winnipeg, MB, Canada

2:00 PM 1-03: Understanding the potential for lignocel-lulose conversion by strains of the cellulolytic thermophile Clostridium clariflavum J.A. Izquierdo*, RTI International, Research Triangle Park, NC; A. Guseva and L.R. Lynd, Dartmouth College, Hanover, NH; S. Pattathil and M.G. Hahn, University of Georgia, Athens, GA

2:30 PM Break/Exhibits open Napoleon Foyer, 3rd fl

3:00 PM 1-04: A new molecular switch for temperature-regulated bioproduct formation in a hyperther-mophilic microorganism M. Basen*, J. Sun and M.W.W. Adams, University of Georgia, Athens, GA

3:30 PM 1-05: Metabolic engineering of a pyruvate metabolic pathway in the hyperthermophilic bacterium, Cadicellulosirutor bescii CBJW018 J. Westpheling*, M. Cha, E. Olivar and D.H. Chung, University of Georgia, Athens, GA, and BioEnergy Science Center, Biosciences Division of DOE, Oak Ridge National Laboratory, Oak Ridge, TN

4:00 PM 1-06: Bacterial community dynamics and gly-coside hydrolase activities during thermophilic biomass deconstruction S.W. Singer*, J.M. Gladden, S.A. Eichorst, P. D’haeseleer and B.A. Simmons, Joint BioEnergy Institute, Emeryville, CA

SESSION 2: Pretreatment & Fractionation I Chair: S.H. Lee, National Institute of Advanced Industrial Science and Technology, Higashi-Hiroshima, Hiroshima, Japan

Rhythms, 2nd fl

1:00 PM 2-01: Optimization and modeling of phosphor-ic-acid-pretreated sugarcane bagasse I.U. Nieves*, C.C. Geddes, R.W. Hoffman, M. Mullinnix and L.O. Ingram, University of Florida, Gainesville, FL

1:30 PM 2-02: Enhancement in enzymatic hydrolysis by mechanical refining of pretreated biomass B. Koo, S. Park*, H. Jameel, R. Phillips and H.M. Chang, North Carolina State University, Raleigh, NC

technical program | 19m

onday, april 30

2:00 PM 2-03: Scale-up and characterization of the cell wall hydrophilicity for alkaline hydrogen peroxide pretreatment at high solids concentrations D. Williams* and D. Hodge, Michigan State University, East Lansing, MI


3:00 PM 2-04: A new low cost design for the AFEX™ process: application in distributed biofuel production systems B.E. Dale*, DOE Great Lakes Bioenergy Research Center, Michigan State University, Lansing, MI; L.D.C. Sousa, S.P. Chundawat and V. Balan, Michigan State University, Lansing, MI; B.D. Bals, T.J. Campbell and F. Teymouri, MBI International, Lansing, MI

3:30 PM 2-05: Optimizing pretreatment for tailor made process equipment M. Pedersen*, H. Buch and M.J. Mikkelsen, BioGasol ApS, Ballerup, Denmark

4:00 PM 2-06: Solid cellulosic sugar production for fuel and chemicals J. Gao*, D. Anderson and B. Levie, Catchlight Energy LLC, Federal Way, WA

SESSION 3: Biomass Supply & Sustainability Chair: K. Tiller, Genera Energy, Knoxville, TN

Waterbury, 2nd fl

1:00 PM 3-01: The US billion-ton update: biomass sup-ply for a bioenergy and bioproducts industry L.M. Eaton*, R.D. Perlack and M. Downing, Oak Ridge National Laboratory, Oak Ridge, TN; B. Stokes, CNJV, LLC, Golden, CO; J. Ferrell, U.S. Department of Energy, Washington, DC

1:30 PM 3-02: The impact of biofuel policy on forest feedstock availability in OECD countries W.E. Mabee*, J. Stephen, K. Calvert, N. Manion, S. Earley, J. Blair and A. Benzie, Queen’s University, Kingston, ON, Canada

2:00 PM 3-03: Genera Energy’s sustainable biomass logistics and preprocessing system S.W. Jackson*, Genera Energy, LLC, Knoxville, TN and K. Tiller, Genera Energy, Knoxville, TN


3:00 PM 3-04: Simulation and optimization of agricul-tural biomass logistics system for cellulosic ethanol production M. Ebadian* and T. Sowlati, The University of British Columbia, Vancouver, BC, Canada; S. Sokhansanj, Oak Ridge National Laboratory, Oak Ridge, TN

3:30 PM 3-05: Indicators to support environmental and socioeconomic sustainability of bioenergy systems V.H. Dale*, L. Baskaran, M. Davis, M. Downing, L. Eaton, R. Efroymson, C. Garten, M. Hilliard, K. Kline, H. Jager, M. Langholtz, P. Leiby, A. McBride, P. Mulholland, G. Oladosu, E. Parish, P. Schweizer and J. Storey, Oak Ridge National Laboratory, Oak Ridge, TN

4:00 PM 3-06: The watershed-scale optimized & rear-ranged landscape design (WORLD) model and local biomass processing depots for biofuel production: a life cycle perspective P.L. Eranki*, B.D. Bals and S. Kim, Biomass Conversion Research Laboratory, Lansing, MI; B.E. Dale, DOE Great Lakes Bioenergy Research Center, Michigan State University, Lansing, MI

Exhibits open Napoleon Foyer, 3rd fl 5:30 PM-9:00 PM

POSTER SESSION 1 Chairs: L. Eaton, Oak Ridge National Laboratory, Oak Ridge, TN and M. Easson, USDA-ARS-SRRC, New Orleans, LA

Napoleon Ballroom C-D, 3rd fl

Sponsored by Energy Biosciences Institute

Sections Plant Science & Technology

Biomass Supply & Sustainability

Biomass Physiochemical Analysis

Biomass Recalcitrance

Pretreatment & Fractionation (I, II, III)

Enzyme Science & Technology

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Tuesday, May 1

Registration Napoleon Foyer, 3rd fl 7:15 AM-5:00 PM

Continental Breakfast-all registered attendees Napoleon Foyer, 3rd fl 7:15 AM-8:00 AM Sponsored by Energy Biosciences Institute


Job Fair Napoleon Foyer, 3rd fl 8:00 AM-4:00 PM

SESSION 4: Bacterial Science & Technology II Chair: J. Peterson, University of Georgia, Athens, GA


8:00 AM 4-01: Thermobifida fusca ß-glucosidase attach-ment to ethanologenic Escherichia coli outer membrane employing the type V secretion system I. Muñoz-Gutiérrez*, G. Gosset and A. Martinez, Instituto de Biotecnología - Universidad Nacional Autónoma de México, Cuernavaca, Mexico

8:30 AM 4-02: Using cellulose without complete hydro-lysis: a new biofuel production paradigm H.D. Shin, R. Sekar and R.R. Chen*, Georgia Institute of Technology, Atlanta, GA

9:00 AM 4-03: Recombinant cellulolytic Bacillus subtilis as a platform for one-step production of biofu-els and biochemical from pretreated biomass X.Z. Zhang*, Virginia Tech; Gate Fuels Inc., Blacksburg, VA; C. You and P. Zhang, Virginia Tech University, Blacksburg, VA; H. Ma, Gate Fuels Inc, Blacksburg, VA

9:30 AM Break/Exhibits open Napoleon Foyer, 3rd fl

10:00 AM 4-04: Lipid production in Rhodobacter sphaer-oides: from photosynthetic membranes to biofuels K.C. Lemmer* and T.J. Donohue, University of Wisconsin, Madison, WI

10:30 AM 4-05: Transcriptome of Zymomonas mobilis 8b to acetate in mixed sugars S. Yang, M.A. Franden, Y.C. Chou, P.T. Pienkos and M. Zhang*, National Renewable Energy Laboratory, Golden, CO; S.D. Brown, Oak Ridge National Laboratory, Oak Ridge, TN

11:00 AM 4-06: Genetic modification of E. coli KO11 for improved ethanol yield from gluconate A. Hildebrand*, J. Fan and T. Kasuga, University of California, Davis, Davis, CA

SESSION 5: Emerging Biofuels Chair: A. Falender, Shell, Houston, TX

Rhythms, 2nd fl

Sponsored by Gevo, Inc.

8:00 AM 5-01: Can ethanol be converted into a compo-nent for blending into diesel fuel? D.K. Johnson* and R. McCormick, National Renewable Energy Laboratory, Golden, CO

8:30 AM 5-02: Extractive fermentation to facilitate catalytic conversion of the ABE fermentation products to fungible fuels Z.C. Baer*, A. Pazhamalai, F.D. Toste, D.S. Clark and H.W. Blanch, UC Berkeley, Berkeley, CA

9:00 AM 5-03: Comparison of tetrahydrofuran and methyl isobutyl ketone solvent systems for enhanced furfural production from cellulosic biomass C.M. Cai*, T. Zhang and C.E. Wyman, Center for Environmental Research and Technology, Riverside, CA


10:00 AM 5-04: Towards cost-efficient biohydrogen pro-duction on switchgrass utilizing a thermophilic strain of Caldicellulosiruptor saccharolyticus S. Talluri, M.R. Subramanian and L.P. Christopher*, South Dakota School of Mines and Technology, Rapid City, SD

technical program | 21tuesday, m

ay 1

10:30 AM 5-05: Aqueous phase depolymerization and hydrodeoxygenation of lignin to jet fuel D.D. Laskar*, L. Yan and B. Yang, Washington State University, Richland, WA

11:00 AM 5-06: Production of biodiesel from residual coconut oil using chitosan as heterogeneous catalyst: an optimized process F.D.F. Souza, H.D.P. Nunes, Z.M. dos Santos and I.L. Lucena, Universidade Federal Rural do Semi-Arido, Mossoro, Brazil; A.D.T. Pinheiro and M.V.P. Rocha*, Universidade Federal do Ceará, Fortaleza, Brazil

SESSION 6: Biomass Physicochemical Analysis Chair: K. Chambliss, Baylor University, Waco, TX

Waterbury, 2nd fl

8:00 AM 6-01: NMR high-throughput screening of hy-drolysates for determination of sugar release E. Gjersing*, R. Sykes, R. Happs, C. Doeppke and M. Davis, National Renewable Energy Laboratory, Golden, CO

8:30 AM 6-02: Estimation of biomass composition and saccharification kinetics using Fourier trans-form infrared spectroscopy P. Varanasi* and S. Singh, Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Emeryville, CA; M. Auer, Joint BioEnergy Institute, Berkeley, CA; P. Adams, Joint BioEnergy Institute, Emeryville, CA; B.A. Simmons, Joint Bioenergy Institute, Emeryville, CA

9:00 AM 6-03: Evaluating direct infusion electrospray ionization - ion mobility - high resolution mass spectrometry (DIESI-IM-HRMS) as a technique for rapid characterization of potential biopro-cess streams C. Becker*, S. Munisamy and C.K. Chambliss, Baylor University, Waco, TX


10:00 AM 6-04: Structural characterization of the impacts of alkaline hydrogen peroxide pretreatment on bioenergy feedstocks exhibiting diverse cell wall phenotypes M. Li*, C.E. Foster and D. Hodge, Michigan State University, East Lansing, MI; S. Pattathil and M.G. Hahn, University of Georgia, Athens, GA

10:30 AM 6-05: Evaluation of sugarcane fibers using modern optical and spectroscopic methods P. Maziero*, Engineering School of Lorena - University of São Paulo, Lorena, Brazil; M. Oliveira Neto, University of Sao Paulo, Sao Carlos, Brazil; M. Ermeydan, M. Eder and P. Fratzl, Max-Planck Institute of Colloids and Interfaces, Potsdam, Germany; I. Polikarpov, Instituto de Fisica de São Carlos (IFSC), Universidade de São Paulo (USP), São Carlos, SP, Brazil; A.R. Gonçalves, University of São Paulo and Brazilian Bioethanol Science and Technology Laboratory, Lorena and Campinas, Brazil

11:00 AM 6-06: Real-time imaging of plant cell wall structure at nanometer scale, with respect to cellulase accessibility and degradation kinetics Y.S. Liu, Y. Zeng, J. Baker, Q. Xu, M. Tucker, M.E. Himmel and S.Y. Ding*, National Renewable Energy Laboratory, Golden, CO; S. Smith, South Dakota School of Mines and Technology, Rapid City, SD; X.S. Xie, Harvard University, Cambridge, MA; E.A. Bayer, Weizmann Institute of Science, Rehovot, Israel

Lunch on your own 11:30 am – 1:00 pm

SESSION 7: Enzyme Science & Technology I Chair: J.N. Saddler, University of British Columbia, Vancouver, BC, Canada

Waterbury Ballroom, 2nd fl

1:00 PM 7-01: A multiplexed, high-throughput screen-ing pipeline for lignocellulosic enzyme discov-ery and evolution R.A. Heins*, X. Cheng, K. Deng, S. Yilmaz, A. Singh, P. Adams, B. Simmons, K.L. Sale and T. Northen, Joint BioEnergy Institute, Berkeley, CA

1:30 PM 7-02: Discovery of novel cellulases and hemicel-lulases from unique metagenomic libraries P. Bromann, University of Helsinki, Helsinki, Finland; S. Ellilä, K. Marjamaa, M. Nyyssönen, M. Itävaara, A. Koivula and K. Kruus*, VTT, Espoo, Finland

2:00 PM 7-03: Linking Trichoderma reesei cultivation process parameters, enzyme production profile and hydrolysis performance L. Lehmann and T. Hobley, Technical University of Denmark, Kgs. Lyngby, Denmark; A. Peciulyte

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and L. Olsson*, Chalmers University of Technology, Gothenburg, Sweden; T. Larsson, Innventia, Stockholm, Sweden; S.M. Stocks and H.S. Jorgensen, Novozymes A/S, Bagsvaerd, Denmark


3:00 PM 7-04: GH61-catalyzed oxidative cleavage of cellulose W. Beeson IV, C. Phillips and M. Marletta*, University of California - Berkeley, Berkeley, CA

3:30 PM 7-05:Recent insights into the enzymatic conver-sion of cellulose and other recalcitrant polysac-charides Z. Forsberg, P.B. Pope, G. Vaaje-Kolstad, B. Westereng, A. MacKenzie, M. Sørlie, S.J. Horn and V.G.H. Eijsink*, Norwegian University of Life Sciences, Aas, Norway

4:00 PM 7-06:New commercial enzymes for lignocellulos-ic biomass degradation: are they good enough? P.V. Harris*, Novozymes, Inc., Davis, CA

SESSION 8: Pretreatment & Fractionation II Chairs: B.E. Dale, DOE Great Lakes Bioenergy Research Center, Michigan State University, Lansing, MI

Napoleon Ballroom A-B, 3rd fl

1:00 PM 8-01: Compositional changes and enzymatic cellulose convertibility of grass ensiled at dif-ferent conditions of inoculation and dry matter concentrations M. Jensen* and J.E. Schmidt, Technical University of Denmark (DTU), Roskilde, Denmark; T. Didion, DLF TRIFOLIUM A/S, Store Heddinge, Denmark; K.S. Johansen, Novozymes A/S, Bagsværd, Denmark

1:30 PM 8-02: Recent achievements in the production of fuel ethanol from sugarcane bagasse L.P. Ramos*, A.P. Pitarelo, R.S. Aguiar, A. Zandona Filho and M.H.L. Silveira, Federal University of Paraná, Curitiba, Brazil; L. Roseiro and F.M. Gírio, LNEG - Laboratório Nacional de Energia e Geologia, Lisboa, Portugal

2:00 PM 8-03: Physical and chemical characterization of sweet sorghum bagasse pretreated with dilute acid, alkaline and liquid hot water technologies J. Li*, Z. Yan, B. Han and S. Li, Institute of New Energy Technology, Beijing, China; Q. Sun, College of chemical engineering, Beijing, China


3:00 PM 8-04: Shock pretreatment M.T. Holtzapple*, Texas A&M University, College Station, TX

3:30 PM 8-05: Investigation of lignin deposition on cel-lulose during hydrothermal pretreatment, its effect on cellulose hydrolysis, and underlying mechanisms H. Li*, R. Kumar and C.E. Wyman, University of California, Riverside, Riverside, CA; Y. Pu and A.J. Ragauskas, Georgia Institute of Technology, Atlanta, GA

4:00 PM 8-06:Mechanistic modeling of lignocellulosic biomass pretreatment and enzymatic hydroly-sis to enable virtual engineering A.J. Griggs, A. Nag, M.A. Sprague, J.J. Lischeske, E.M. Kuhn, M.P. Tucker and J.J. Stickel*, National Renewable Energy Laboratory, Golden, CO

SESSION 9: Biobased Chemicals Chair: J.O. Rich, National Center for Agricultural Utilization Research, USDA - Agricultural Research Service, Peoria, IL

Rhythms, 2nd fl

Sponsored by Bird Engineering and Vogelbusch GMBH

1:00 PM 9-01: Integrating biological and chemicals catalysts for biobased chemical production B.H. Shanks*, Iowa State University, Ames, IA

1:30 PM 9-02: Optimization of simultaneous reaction and solvent extraction to enhance yields of furfural and other derivatives from cellulosic biomass T. Zhang*, C.M. Cai, R. Kumar and C.E. Wyman, Center for Environmental Research and Technology, Riverside, CA

2:00 PM 9-03: High-yield production of lactic acid and meso-2,3-butanediol from cellodextrin by engineered E. coli biocatalysts: toward consoli-dated production from cellulose H.D. Shin, C. Rutter, J. Wu and R. Chen*, Georgia Institute of Technology, Atlanta, GA; S.H. Yoon and S.W. Kim, Gyeongsang National University, Jinju, South Korea


technical program | 23tuesday, m

ay 1

3:00 PM 9-04: The design, engineering, and optimiza-tion of a microbial process for manufacturing of 1,4-butanediol H. Yim*, Genomatica, San Diego, CA

3:30 PM 9-05: Whole-cell biocatalytic production of 2,5-furan dicarboxylic acid (FDCA) T.D. Elink Schuurman and H.J. Ruijssenaars*, BIRD Engineering B.V., Schiedam, Netherlands

4:00 PM 9-06: Challenges and progress in the rapid commercial development of sustainable chemicals D. Greenfield* and K. Hu, LS9, South San Francisco, CA

Exhibits Open Napoleon Foyer, 3rd fl5:30 PM-9:00 PM

POSTER SESSION 2 Chairs: C. Dibble, NREL, Golden, CO and A. Engleberth, Purdue University, W. Lafayette, IN

Napoleon Ballroom C-D, 3rd fl 6:00 PM-9:00 PM

SectionsBacterial Science & Technology

Yeast and Fungal Science & Technology

Algae Science & Technology

Biobased Chemicals

Emerging Biofuels

Bioprocessing & Separations Technology

Biofuels and Biorefinery Economics & Commercialization

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Wednesday, May 2


Continental Breakfast-all registered attendees Napoleon Foyer, 3rd fl 7:15 AM-8:00 AM


SESSION 10: Enzyme Science & Technology II Chair: M.R. Nimlos, National Renewable Energy Laboratory, Golden, CO


8:00 AM 10-01: Computational enzyme design: a tool for rapidly designing new enzymatic activities A. Zanghellini*, Arzeda Corporation, Seattle, WA

8:30 AM 10-02: Initial- and processive-cut products from cellobiohydrolase-catalyzed hydrolysis of cellulose reveal rate-limiting steps and role of companion enzymes J. Fox*, S.E. Levine, D.S. Clark and H. Blanch, University of California, Berkeley, Berkeley, CA

9:00 AM 10-03: Studies of synergism between two Thermobifida fusca cellulases, Cel48A and Cel9A, via empirical modeling and site directed mutagenesis M. Kostylev*, M. Chen, J.W. Brady and D. Wilson, Cornell University, Ithaca, NY; M.E. Himmel, National Renewable Energy Laboratory, Golden, CO


10:00 AM 10-04: Hydrogen deuterium exchange mass spectrometry-based enzyme dynamics analysis revealed new structure determinants and mechanisms U. Uzuner*, W. Shi, S.Y. Dai and J.S. Yuan, Texas A&M University, College Station, TX; H. Guo, University of Tennessee, Knoxville, TN

10:30 AM 10-05: Probing cellulase binding on crystalline cellulose surfaces using large-scale molecular dynamics simulations G. Bellesia* and S. Gnanakaran, Los Alamos National Laboratory, Los Alamos, NM; S.P. Chundawat, Michigan State University, Lansing, MI; M. Crowley and G. Beckham, National Renewable Energy Laboratory, Golden, CO; B.E. Dale, DOE Great Lakes Bioenergy Research Center, Michigan State University, Lansing, MI

11:00 AM 10-06: Understanding fungal cellulases with molecular simulation G.T. Beckham*, L. Bu, C.M. Payne, D.W. Sammond, M. Nimlos, C.J. Dibble, M.E. Himmel and M.F. Crowley, National Renewable Energy Laboratory, Golden, CO; C.B. Taylor and C. McCabe, Vanderbilt University, Nashville, TN; Y. Lin and J.W. Chu, University of California Berkeley, Berkeley, CA

SESSION 11: Biomass Recalcitrance Chair: M. Pauly, UC Berkeley / Energy Biosciences Institute, Berkeley, CA


8:00 AM 11-01: Assessing the cellulase accessibility of lignocellulosic substrate before and after pre-treatment X. Meng, M. Foston and A. Ragauskas*, BioEnergy Science Center/Georgia Institute of Technology, Atlanta, GA; C. Wyman and J. DeMartini, University of California, Riverside, Riverside, CA

8:30 AM 11-02: Macromolecular modeling of cellulose microfibrils in deconstructed biomass from transmission electron tomography P.N. Ciesielski*, J.F. Matthews, M.P. Tucker, M.F. Crowley, M.E. Himmel and B.S. Donohoe, National Renewable Energy Laboratory, Golden, CO

9:00 AM 11-03: Comparative glycomics of plant biomass and insights into cell wall components that af-fect recalcitrance S. Pattathil*, J. Miller, V. Brown, S. Kandemkavil, A.K. Biswal, T. Saffold, M. O’Neill, W.S. York, D. Mohnen and M. Hahn, University of Georgia, Athens, GA; S.P.S. Chundawat and B.E. Dale, DOE Great Lakes Bioenergy Research Center, Michigan State University, Lansing, MI; J. DeMartini and H. Li, University of California, Riverside, Riverside, CA; C.E. Wyman, University of California Riverside, BioEnergy Science Center, Riverside, CA; R.A. Dixon and F. Chen, Samuel Roberts Noble Foundation, Ardmore, OK

technical program | 25w

ednesday, may 2


10:00 AM 11-04: Polysaccharide O-acetylation and wall recalcitrance M. Pauly*, S. Gille, A. de Souza and G. Xiong, UC Berkeley / Energy Biosciences Institute, Berkeley, CA

10:30 AM 11-05: Lignin and ABC transport modification to reduce recalcitrance A. Ziebell*, R. Sykes, B. Donohoe, S.R. Decker and M.F. Davis, National Renewable Energy Laboratory, Golden, CO; D. Badri and J. Vivanco, Colorado State University, Ft. Collins, CO; F. Chen and R.A. Dixon, Samuel Roberts Noble Foundation, Ardmore, OK

11:00 AM 11-06: Varied recalcitrance in sugar cane hy-brids with contrasting lignin contents A. Ferraz*, F. Masarin, T.H. Costa, F.M. Mendes, D.F. Laurito, W. Carvalho and A.M. Milagres, Escola de Engenharia de Lorena - USP, Lorena, Brazil

SESSION 12: Algae Science & Technology Chair: R. Sayre, Los Alamos National Laboratory, Los Alamos, NM

Rhythms, 2nd fl

8:00 AM 12-01: A model alga for oil production: Nannochloropsis gaditana draft genome, genetic transformation, and high lipid productivities R.E. Jinkerson*, R. Radakovits and M.C. Posewitz, Colorado School of Mines, Golden, CO

8:30 AM 12-02: Development of a genome-scale meta-bolic model for cyanobacterial metabolism T.T. Vu* and J.L. Reed, University of Wisconsin-Madison, Madison, WI; S.M. Stolyar, G.E. Pinchuk, A.E. Konopka and A.S. Beliaev, Pacific Northwest National Laboratory, Richland, WA

9:00 AM 12-03: Metabolic engineering of green algae for autotrophic production of terpenoid hydrocarbons U. Uzuner*, R. Syrenne, S. Xie, S.Y. Dai and J.S. Yuan, Texas A&M University, College Station, TX


10:00 AM 12-04: A hetero-photoautotrophic two-stage cultivation model to improve wastewater nutri-ent removal and enhance algal lipid accumula-tion for low cost biofuel feedstock production

W. Zhou*, M. Min, Y. Li, B. Hu, P. Chen and R. Ruan, University of Minnesota, Saint Paul, MN

10:30 AM 12-05: Resolving complex microbial interac-tions for sustainable production of algae C.E. Bagwell* and C.E. Milliken, Savannah River National Laboratory, Aiken, SC; M. Piskorska, University of South Carolina, Aiken, SC

11:00 AM 12-06: Bioconversion of Nannochloropsis salina lipid-extracted biomass into ethanol M. Mirsiaghi, J. Roth and K.F. Reardon*, Colorado State University, Fort Collins, CO

Free Afternoon

Organizing Committee Luncheon Meeting Maurepas, 3rd fl 12:15 PM-1:45 PM

SESSION ST: Special Topic: US & International Bioenergy Research Center Updates Chair: B.H. Davison, Oak Ridge National Laboratory, Oak Ridge, TN


7:00 PM ST-01: The UK BBSRC Sustainable Bioenergy Centre (BSBEC) A. Karp*, Rothamsted Research, Harpenden, United Kingdom, P. Dupree, University of Cambridge, Cambridge, United Kingdom, C. Halpin, University of Dundee, Dundee, United Kingdom, S. McQueen-Mason, University of York, York, United Kingdom, N. Minton, University of Nottingham, Nottingham, United Kingdom and K. Smart, University of Nottingham, United Kingdom

7:20 PM ST-02: Production technology of clean biofuels from lignocellulosic biomass resources K. Sakanishi*, National Institute of Advanced Industrial Science and Technology, Higashi-Hiroshima, Japan

7:40 PM ST-03: The BioEnergy Science Center: update on progress P. Gilna*, Oak Ridge National Laboratory, Oak Ridge, TN

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8:00 PM ST-04: Beyond ethanol: advanced biofuel re-search at the Joint BioEnergy Institute B.A. Simmons*, H. Scheller, P. Adams, H. Blanch and J. Keasling, Joint BioEnergy Institute, Emeryville, CA

8:20 PM ST-05: Great Lakes Bioenergy Research Center: producing sustainable cellulosic biofuels T. Donohue*, University of Wisconsin, Madison, WI

8:40 PM ST-06: The Energy Biosciences Institute: an update H.L. Youngs*, Energy Biosciences Institute, Berkeley, CA

9:00 PM Panel Discussion

technical program | 27thursday, m

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Thursday, May 3


Continental Breakfast-all registered attendees Napoleon Foyer, 3rd fl 7:15 AM-8:00 AM


SESSION 13: Bioprocessing & Separations Technology Chair: B. Kamm, Research Institute Biopos eV. and BTU Cottbus, Teltow, Germany

Rhythms, 2nd fl

8:00 AM 13-01: Alleviating monoterpene toxicity using a two-phase extractive fermentation for the bioproduction of jet fuel mixtures in Saccharomyces cerevisiae T. Brennan*, C. Turner, J. Kromer and L.K. Nielsen, University of Queensland, Brisbane, Australia

8:30 AM 13-02: Screening of enzymes and reaction parameters under process conditions using microsystems J. Fagaschewski, J. Simons, K. Götz and L. Hilterhaus*, Hamburg University of Technology, Hamburg, Germany

9:00 AM 13-03: Acid catalyzed hydrolysis of switchgrass in ionic liquid and separation of sugars using liquid-liquid extraction N. Sun*, K. Tran, A. George, B.A. Simmons and B.M. Holmes, Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Emeryville, CA

9:30 AM Break Napoleon Foyer, 3rd fl

10:00 AM 13-04: Continuous pilot scale process demon-strating waste fibre as a feasible feedstock to ethanol and biogas production K. Kemppainen*, A. Kallioinen, M. Siika-aho, K. Sipilä

and N. von Weymarn, VTT Technical Research Centre, Espoo, Finland; L. Ranta, UPM-Kymmene Oyj; E. Sipilä, Pöyry Management Consulting Oy; A. Östman, Skandinavisk Kemiinformation AB; J. Vehmaanperä, ROAL Oy, Rajamäki, Finland; T. Puranen, Roal Oy, Rajamäki, Finland; K. Langfelder, AB Enzymes GmbH; J. Hannula, Lassila & Tikanoja Oyj

10:30 AM 13-05: Performance evaluation and fouling analysis of reverse osmosis membranes and evaporator heating surfaces during concentra-tion of enzymatic hydrolysate sugars T.J. Menkhaus*, R. Guarram and A. Gautam, South Dakota School of Mines and Technology, Rapid City, SD

11:00 AM 13-06: A process for the direct fermentation of lignocellullosic material to ethanol in a multi-species membrane reactor S. Brethauer* and M.H.P. Studer, ETH Zurich, Zurich, Switzerland

SESSION 14: Pretreatment & Fractionation III Chair: L.P. Ramos, Federal University of Paraná, Curitiba, Brazil


8:00 AM 14-01: Producing high sugar yields from soft-wood using wet explosion pretreatment B.K. Ahring*, D. Rana, V. Rana and P. Teller, Washington State University, Richland, WA

8:30 AM 14-02: Biomass pretreatment by a continuous flow of superheated steam J.W. van Groenestijn* and H.C. van Deventer, TNO, Zeist, Netherlands

9:00 AM 14-03: Extractive-AFEX (E-AFEXTM) pretreat-ment: a unified approach for resolving bottlenecks to efficient cellulosic bioethanol production L. da Costa Sousa*, Michigan State University, E. Lansing, MI; M. Jin, N. Uppugundla, J.F. Humpula, C. Gunawan, S.P.S. Chundawat, B.E. Dale and V. Balan, DOE Great Lakes Bioenergy Research Center, Michigan State University, Lansing, MI; V. Bokade, National Chemical Laboratory, Pune, India; M.B. Foston, Georgia Institute of Technology, Atlanta, GA; A. Azarpira and J. Ralph, DOE Great Lakes Bioenergy Center, University of Wisconsin, Madison, WI; B.D. Bals and F. Teymouri, MBI International, Lansing, MI


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10:00 AM 14-04: Cellulose solvent-based lignocellulose pretreatment enables efficient pretreatment of mixed feedstocks: Miscanthus and poplar N. Sathitsuksanoh*, N. Sun, B.A. Simmons and A. George, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Emeryville, CA; Y.H.P. Zhang, Virginia Tech, Blacksburg, VA

10:30 AM 14-05: Use of a twin-screw extruder as a mixing tool to promote sugarcane bagasse pretreat-ment with ionic liquids at high solid contents A.S. da Silva*, R.S.S. Teixeira and E.P.S. Bon, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; T. Endo and S.H. Lee, National Institute of Advanced Industrial Science and Technology, Higashi-Hiroshima, Japan

11:00 AM 14-06: Novel ionic liquid and water mixtures for biomass dissolution A. George*, Z. Chen, N. Sun, S. Singh, K. Sale, B. Simmons and B.M. Holmes, Joint BioEnergy Institute, Emeryville, CA; V. Stavila, Sandia National Laboratories, Livermore; K. Tran, Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Emeryville, CA; N. Mac Dowell, Imperial College London; T. Welton and J.P. Hallett, Imperial College London, London, United Kingdom

SESSION 15: Plant Science & Technology Chair: R. Johnson, USDA-FS, Arlington, VA

Waterbury, 2nd fl

8:00 AM 15-01: Crown and rhizome transcriptomes from upland and lowland tetraploid switchgrasses G. Sarath*, N.A. Palmer, A.J. Saathoff and K.P. Vogel, USDA-ARS, Lincoln, NE; C. Tobias, USDA, Agricultural Research Service, Albany, CA; P. Twigg, University of Nebraska, Kearney, Kearney, NE; M. Soundararajan, University of Nebraska, Lincoln, NE

8:30 AM 15-02: A systems approach to reducing biomass recalcitrance by modifying glucuronoxylan biosynthesis B. Urbanowicz*, M.J. Pena, J. Backe, S. Ratnaparkhe, U. Avci, M.A. O’Neill and W.S. York, University of Georgia, Athens, GA

9:00 AM 15-03: Enhanced hydrolysis of lignocellulosic feedstocks through in planta expression of cell wall degrading enzymes P.A. Lessard*, Agrivida, Inc., Medford, MA


10:00 AM 15-04: Elucidating the genetic basis of sugar accumulation in sweet sorghum using high-throughput gene expression profiling of heterogeneous inbred families A. Saballos, J. Fear and W. Vermerris*, University of Florida, Gainesville, FL; T. Felderhoff, University of Florida Genetics Institute, Gainesville, FL; S. Murray and W.L. Rooney, Texas A&M University, College Station, TX; S. Kresovich, University of South Carolina, Columbia, SC

10:30 AM 15-05: Improved biomass and bioprocessing properties of lignocellulosic forest feedstocks E. Mellerowicz, B. Sundberg and H. Tuominen*, Umeå Plant Science Centre, Umeå, Sweden; L. Jönsson, Umeå University, Umeå, Sweden

11:00 AM 15-06: Identification and characterization of Cal-1, a high glucan maize mutant M. Pauly*, F. Kraemer and B. Kuhn, UC Berkeley / Energy Biosciences Institute, Berkeley, CA; S. Hake, USDA Albany, Albany, CA

SESSION 16: Enzyme Science & Technology III Chair: K. Kruus, VTT, Espoo, Finland


1:00 PM 16-01: Crystallinity – a key parameter for cel-lulosic biomass hydrolysis Y. Kang*, P. Bansal, B.J. Vowell, M.J. Realff and A.S. Bommarius, Georgia Institute of Technology, Atlanta, GA

1:30 PM 16-02: The need to increase the specific surface area of cellulose: how pretreatment and the nature of the “cellulase complex” are critical to obtaining high-yield sugar streams from ligno-cellulosic substrates when using low enzyme dosages V. Arantes*, R. Chandra and J. Saddler, University of British Columbia, Vancouver, BC, Canada

2:00 PM 16-03: Physical factors limiting bioconversion at high solids: the role of water C.W.C. Hsieh*, C. Felby and L.G. Thygesen, University of Copenhagen, Frederiksberg , Denmark

2:30 PM Break Napoleon Foyer, 3rd fl

technical program | 29thursday, m

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3:00 PM 16-04: Hydrolysis of steam-treated sugarcane bagasse at high total solids and low enzyme loadings L. da Silva, A.C. Ballem, M.H.L. Silveira and L.P. Ramos*, Federal University of Paraná, Curitiba, Brazil

3:30 PM 16-05: Recyclable enzymes for hydrolysis of lignocellulose A. Várnai* and L. Viikari, University of Helsinki, Helsinki, Finland; M. Siika-aho, VTT Technical Research Centre of Finland, Espoo, Finland

4:00 PM 16-06: Processing techniques for reducing the cost of enzymatic hydrolysis X. Li, B. Emme*, L. Putnam, C. Corbett, K. Brandon Sutton, J. Frickmann and M. Torry Smith, Novozymes North America, Inc., Franklinton, NC

SESSION 17: Yeast and Fungal Science & Technology Chair: H.J. Ruijssenaars, BIRD Engineering B.V., Schiedam, Netherlands


1:00 PM 17-01: A genome-wide approach to understand the roots of cellulase hyper-secretion in the filamentous fungus Trichoderma reesei T. Portnoy, S. Blanquet, F. Monot and A. Margeot*, IFP Energies nouvelles, Rueil-Malmaison, France; F. Bidard-Michelot, École Normale Supérieure, Paris, France; B. Seiboth and C.P. Kubicek, Vienna University of Technology, Vienna, Austria; S.E. Baker, Pacific Northwest National Laboratory, Richland, WA; S. Le Crom, Université Pierre et Marie Curie-Paris 6, Paris, France

1:30 PM 17-02: Engineering yeasts for next generation ethanol production R. den Haan*, D. La Grange, H. Kroukamp, M. Saayman, M. Viktor, M. Bloom and W.H. Van Zyl, Stellenbosch University, South Africa; J. McBride and L.R. Lynd, Mascoma Corporation, Lebanon, NH; M. Ilmen and M. Penttilä, VTT Technical Resesarch Centre of Finland; J.F. Gorgens, Stellenbosch University, Stellenbosch, South Africa

2:00 PM 17-03: Spathaspora passalidarum co-ferments glucose, xylose and cellobiose in minimal me-dium and hydrolysates T.W. Jeffries* and L.B. Willis, USDA Forest Service, Madison, WI; T.M. Long, Y.K. Su and A.J. Higbee, University of Wisconsin-Madison, Madison, WI; J.R. Headman, Verdezyne, Inc., Carlsbad, CA


3:00 PM 17-04: Engineering an alternative glyco-lytic pathway that increases ethanol yield in Saccharomyces cerevisiae J.R. Headman*, E. Scott, J. Laplaza, K. Aeling, A. Hutagalung and S. Picataggio, Verdezyne, Inc., Carlsbad, CA

3:30 PM 17-05: Phenotypic and multiomic approaches to understanding molecular bottlenecks in the fermentation of lignocellulosic biomass by Saccharomyces cerevisiae T.K. Sato*, D.J. Wohlbach, J.A. Lewis, Y. Zhang, Y. Bukhman, J. Reed, K. Richmond, R. Landick and A.P. Gasch, University of Wisconsin, Madison, WI; W. Schackwitz and C. Pennacchio, DOE Joint Genome Institute, Walnut Creek, CA; D. Hodge and V. Balan, Michigan State University, East Lansing, MI

4:00 PM 17-06: Production of itaconic acid from biomass using Ustilago maydis L.M. Blank*, N. Wierckx, N. Maassen, E. Geiser, M. Panakova, M. Zimmermann and B. Ebert, RTWH Aachen University, Aachen, Germany

SESSION 18: Biofuels and Biorefinery Economics & Commercialization Chair: N. Kolhapure, DuPont, Wilmington, DE

Rhthyms, 2nd fl

Sponsored by Abengoa Bioenergy New Technologies

1:00 PM 18-01: Restarting the Chinese biobutanol industry E.T. Davies*, R. Dominguez, R. Gemmell and C.A. Lalander, Green Biologics, Abingdon, United Kingdom

1:30 PM 18-02: Techno-economic analysis of combined bioethanol and biogas production from hemp Z. Barta*, B. Sipos and K. Réczey, Budapest University of Technology and Economics, Budapest, Hungary; E. Kreuger, L. Björnsson, O. Wallberg and G. Zacchi, Lund University, Lund, Sweden

2:00 PM 18-03: Potential configurations of a cellulosic biofuel system - global warming and economic perspectives S. Kim and D.E. Bruce*, Michigan State University, East Lansing, MI

30 | technical programth

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3:00 PM 18-04: Scaling up the Borregaard BALI™ concept M. Lersch*, Borregaard Ind. Ltd., Sarpsborg, Norway

3:30 PM 18-05: KL Energy: industrial scale conversion of biomass to sugars and ethanol K. Flanegan*, R. Zebroski, L. Tenlep, T. Kennedy, D. Burke, J. Schultze and P. Gross, KL Energy Corporation, Rapid City, SD

4:00 PM 18-06: Production of the sustainable renewable biofuels farnesane from US domestic sweet sorghum J. Kim*, K. Yu, J. Leng, M. Santolini, J. Weisshar, J. Castle, D. Gray, S. Sato, T.S. Bass, W. Ramirez, G. Dorin, R. Henry, T. Pray, R. Eppler, E. Bellisimi and P. Poon, Amyris, Emeryville, CA; S. Harris and J.P. Rebolledo, Ceres Inc, Thousand Oaks, CA; A. Chapeaux, National Renewable Energy Laboratory, Golden, CO

Banquet Reception 6:00 PM-7:00 PM Lagniappe, 2nd fl

Symposium Banquet and Award Presentations 7:00 PM Armstrong Ballroom, 8th fl

Sponsored by Novozymes

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Recent Advances in Fermentation Technology (RAFT X) Fall 2013

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