4 C -ImmunoAssay protocols and machine cycles

ImmunoAssay protocols and machine cycles

• The protocol of each analyte specifies all the steps executed by the machine (times, volumes, temperatures) and their order.

• All the steps performed by the instrument during the execution of any analyte (dispensing, washing, aspirating, reading), have a specific time length.

• The time length is quantified by the instrument in machine-cycles. Each machine cycle is 320 seconds for IA and 70 seconds for CC/SP.

• There are currently 10 different protocols for all immunoassay tests.

Immunoassays sorted by protocol (1)

Protocol type Analyte Code

1 HCG 1703000 39 min

2 A - TPO 1707100 45 min

2 AFP 1707600 45 min

2 CORTISOL 1703900 45 min

2 F-PSA 1706500 45 min

3 FSH 1701000 51 min

3 LH 1702000 51 min

3 PRL 1708000 51 min

4 FERRITIN 1709500 52 min

4 F-T3 1708600 52 min

4 F-T4 1708500 52 min

4 PROG. 1702700 52 min

4 T3 1708400 52 min

Protocol type Analyte Code

5 BCA (Ca 15-3) 1705400 76 min

5 C-PEPTIDE 1702800 76 min

5 GIC (Ca 19-9) 1705300 76 min

5 INSULIN 1706200 76 min

5 OVC (Ca 125) 1705500 76 min

5 PSA 1706400 76 min

5 SHBG 1705800 76 min

5 TSH 1707300 76 min

6 F-E3 1703500 76 min

7 ANDROSTENEDIONE* 1700100 64 min

7 CEA 1706600 64 min

7 DHEA-S 1700300 64 min

8 E2 1702600 98 min

9 A - hTG 1707000 80 min

9 TESTOSTERONE 1706000 80 min

10 T4 1708200 64 min

Immunoassays sorted by protocol (2)