132 PATENT ABSTRACTS

mutant F.1, 416 or Streptomyces peucetius vat. caesius. The new compounds are useful against both gram positive and gram nega- tive bacteria and as antitumor agents.

4370417

RECOMBINANT DEOXYRIBO- NUCLEIC ACID WHICH CODES

FOR PLASMINOGEN ACTIVATOR

Paul P. Hung, Shaw-Guang Lee, Ranajit Roychoudhury, Barry J. Ratzkin, W. Jur- gen Schrenk, Michael C. Chen, assigned to Abbott Laboratories

The present invention provides a deoxyribo- nucleic acid (DNA) segment related to a human plasminogen activator gene. The segment is inserted into a plasmid vector which in turn can be incorporated into a bacterium or other microorganism. The bac- terium can then be cultured to produce a plasminogen activator protein having prop- erties of human urokinase.

4370413

MICROMETHOD FOR THE DETERMINATION OF ENDOTOXINS

Ishak Neeman, Stephen Gaffin, Haifa, Israel, assigned to Technion Research and Devel- opment Foundation Lid

An improved micromethod for the quan- titative determination of endotoxins with Limulus Amebocytc Lysate (L.A.L.) reagent

is based on the discovery that if L . A i . rea- gent and a sample containing microquanti- ties of endotoxin are incubated in a capillary tube, a clot will be formed in the plug pre- venting any outflow of liquids when low hydrostatic pressure is applied. Increasing the pressure will eventually force out the clot plugging the capillary and the liquid will suddenly erupt giving a clear yes-no indication of the presence of endotoxin. The hydrostatic pressure indicated to cause this eruption is a relatively precise indication of the extent of clotting which, in turn, is indicative of the concentration of endotoxin in the liquid. A kit which can be used to perform this test is also disclosed.

4370317

PANCREATIC SPASMOLYTIC POLYPEPTIDE

Klavs H. Jorgensen, Karin D. Jorgensen, Lars Thim, Virum, Denmark, assigned to Novo Industri A 'S

The invention relates to a novel purified polypeptide which is recoverable from por- cine pancreas glands. The amino acid com- position of the said polypeptide has been determined to be as follows: Trp (2), Lys (4), His (I), Arg (5), Asx (10), Thr (3), Ser (9), Glx (12), Pro (12), Gly (6), Ala (6), Cys I/2 (14), Val (7), Met (2), lie (3), Leu (1), Try (2), Phe (7), wherein the determinations are subjected to the usual error of +/- t0 percent of the indicated figures. The partial amino acid sequence comprising a total of 45 amino acids from the N-terminal, is believed to be: -Ser-Asx-Glx-Cys-30Phe-Thr-Ser- Gly-Cys-35Cys- Phe-Asx-Ser-Glx-40Va L- wherein pyrGlu (residue 1) stands for pyro- glutamic acid. The purified polypeptide is of utility as a medicament, for example as a spasmolytic agent, as an agent for treatment of gastroduodenal ulcers and as a diagnostic aid.