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44 Tumulak, Jamaica Marie M.

2B-PH

ASSIGNMENT IN MICROBIO LAB

DIFFERENT TYPES OF MICROSCOPE

1. SIMPLE MICROSCOPE

I !"i# !$%e &' micrc&%e, !"e c&(e)le# &' #"&r! '&cal le*!" !& #ee ma*i+eima*e &' a #mall &ec!. I! al#& ci#!# &'a ic&(e) le# /"ic" i# m&(e u% a&/ $ !"e u#e &' a au#!me! #cre/!& ri* !"e &ec! i #"ar% '&cu#. T"e&ec! i# %lace & !"e %la!'&rm a li*"!

i# '&cu#e /i!" !"e "el% &' a c&ca(emirr&r. T"i# micrc&%e ca al#& e callea i##ec!i* micrc&%e.

2. LIGHT/COMPOUND MICROSCOPE

T"i# ki &' micrc&%e /"ic" i# /iel$u#e i "i*" #c"&&l a c&lle*ela&ra!&rie# ci#!# &' !/& #e! &' c&(e)le#e#. I! u#e# mul!i%le le#e# '&r !"eelar*eme! &' a #am%le ima*e &r#%ecime. I! al#& ac0uire "i*"er le(el# &'ma*i+ca!i& !"a a #!ere& &r l&/ %&/ermicrc&%e. T"e &ec!i(e le#e# are

ciere a# &e &' !"e m! #i*i+ca!%ar!# &' a c&m%&u micrc&%e, a# !"e$are !"e le#e# cle#! !& !"e #%ecime.

3. TRANSMISSION ELECTRON

MICROSCOPE

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T"i# !$%e &' elec!r& micrc&%e'uc!i uer !"e #ame a#ic%rici%le# a# a &%!ical micrc&%e. I!u!ili1e# (ari&u# eer*e!ic elec!r !&%r&(ie m&r%"&l&*ic, c&m%i!i&al

a cr$#!all&*ra%"ic i'&rma!i& am%le#. Tra#mi##i& elec!r&micrc&%e# ca e ciere a# !"em! %&/er'ul micrc&%e# a! ama)imum %&!e!ial ma*i+ca!i& &' a&me!er. 3&r !"e ee+!# &' !"eeuca!i&al, #cie!i+cal a iu#!riala%%lica!i, !"i# micrc&%e /a#c!ruc!e !& %r&uce "i*"-re#&lu!i&a !/&-ime#i&al ima*e#.

4. SCANNING ELECTRONMICROSCOPE

T"i# ki &' elec!r& micrc&%e i# u#e

!& #!u$ !"e e!aile #ur'ace# &' !"e

cell a &r*ai#m#. I !"i# micrc&%e,

!"e '&rma!i& &' !"e ima*e i# ue !&

!"e elec!r reec!e ack 'r&m !"e

&ec!. T"e ima*e '&rme $ !"i#

micrc&%e "a# a remarkale !"ree

ime#i&al a%%earace. T"e #aimicrc&%e re0uire# a #!ale %&/er

#u%%l$, (acuum a c&&li* #$#!em

a (ira!i&-'ree #%ace. I! i# a mu#!

!"a! #cai* elec!r& micrc&%e# are

"&u#e i a area /"ere i! i# i#&la!e

'r&m amie! ma*e!ic a elec!ric +el#.

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5. PHASE CONTRAST MICROSCOPE

T"i# !$%e &' micrc&%e i# /iel$ u#e !&

#!u$ !"e e"a(i&r &' li(i* cell#,

er(e !"e uclear a c$!&%la#mic

c"a*e# !aki* %lace uri* mi!i# a!"e (ari&u# e5ec!# &' i5ere! c"emical#

i#ie !"e li(i* cell#. I! al#& eale# !"e

(ie/i* &' li(e micr&&r*ai#m#. B$ u#i*

!"e %"a#e-c&!ra#! micrc&%e, a ima*e

&' #!r&* c&!ra#! &' !"e &ec! i#

&!aie. Oe &' !"e ma&r a(a!a*e#

&' %"a#e c&!ra#! micrc&%$ i# !"a! !"e

e)amia!i& &' li(i* cell# i !"eir a!ural

#!a!e ca e /i!"&u! %re(i&u#l$ ei*

kille, +)e, a #!aie.

6. INTERFERENCE MICROSCOPE

I!er'erece micrc&%e u!ili1e# li*"!

i!er'erece %"e&mea '&r !"e crea!i&

&' !/& #u%erim%e ima*e# &' a &ec!

!"a! /ill %a(e !"e /a$ '&r !"e %#ile

er(a!i& &' !ra#%are! &ec!#

/i!"&u! u#i* a #!aii* !ec"i0ue. T"i#

!$%e &' micrc&%e i# al#& u#e '&r

0ua!i!a!i(e #!uie# &' macr&m&lecule#

&' !"e cell c&m%&e!# like li%i#, ucleic

aci# a %r&!ei#.

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PARTS OF A COMPOUND

MICROSCOPE