PATENT ABSTRACTS

Compounds useful as complementary DNA (cDNA) include deoxyribonucleotides and at least one ribonucleotide. They may be depicted by the general formula: See Patent for Chemical Structure wherein (dN)a and (dN)c represent series of deoxyribonucleotides and (rN)b represents a series of ribonucleotides; wherein a, b, and c are the number of nucleotides in the series, with the proviso that b is > or = 1, a is > or =35, and c is > or =10; wherein the series of deoxyribonucleotides (dN)a includes a series of deoxyribonucleotides which is substantially complementary to the series of deoxy- ribonucleotides (dN)c and the dashed line represents noncovalent bonding between the complementary deoxyribonucleotide series; and wherein the solid line represents a covalent phosphodiester bond. These compounds may be prepared from messenger RNA (mRNA) con- taining the genetic information necessary for cel- lular production of desired products such as polypeptides. After appropriate modification, they may be combined with DNA from a suitable cloning vehicle such as a plasmid and the resulting combined DNA used to transform bac- terial cells. The transformed bacterial cells may then be grown and harvested; and the desired product or products recovered.

4504581

M E T H O D F O R P R O D U C I N G L - H I S T I D I N E B Y F E R M E N T A T I O N

Osamu Kurahashi, Takayasu Tsuchida, Hiroki Kawashima, Hitoshi Enei, Shigeru Nakamori, Kawasaki, Japan assigned to Ajinomoto Com- pany Incorporated

L-Histidine producing microorganisms which have been constructed by introducing a recom- binant plasmid DNA inserted on a chromosomal DNA fragment into a recipient strain of the genus Bacillus. These micro- organisms are employed to produce L-histidine in higher than normal yields. The recombinant plasmid DNA inserted is obtained from a donor mutant strain of Bacillus subtilis which is resistant to certain L-histidine antagonists. The resistant plasmid confers the properties of the mutant strain upon the recipient Bacillus subtilis to make its high yield of product even higher,

91

4506013

4503155

M U L T I F U N C T I O N A L , C L O N I N G V E C T O R S F O R U S E I N

S T R E P T O M Y C E S , B A C I L L U S , A N D E. C O L I .... /

James Miller, Jeffrey T Fayerman, Steven Kovacevic, Nancy E Beerman assigned to Eli Lilly and Company

R m ~ k ~ ( m ~ and Fur~llonaJ Map d wa=a~d p U ~

H~lJl ~ m ~ R t

k m M t

~ m

The present invention discloses multifunctional recombinant DNA cloning vectors for use in Streptomyces, Bacillus, and E. coli. The inven- tion further discloses transformants of the af- orementioned vectors.

S T A B I L I Z I N G A N D S E L E C T I N G R E C O M B I N A N T D N A H O S T

C E L L S

Charles L Hershberger, Anna K Radue, Paul Rosteck assigned to Eli Lilly and Company

Restr loUon Si te and I:unctJona| Map of I~asmld p l A 2

itl.O kbl A ~ | Indl~le D~mtlon of T ~ l t t l 4 m

e;e

A method for stabilizing and selecting host cells containing recombinant DNA which expresses a functional polypeptide and the novel organisms and cloning vectors for the practice thereof. The invention further provides a simple, convenient, and inexpensive method to lyse host cells for purification of intracellular products.