PATENT ABSTRACTS

I n s t r u m e n t a t i o n , A s s a y a n d E q u i p m e n t Design

4530786

A N T I B O D Y F O R T H E D E T E C T I O N A N D

Q U A N T I F I C A T I O N O F A T R A Z I N E

Bohn D Dunbar, Gordon D Niswender, James Hudson assigned to Colorado State University Research Foundation

This invention relates to an antibody capable of both specifically detecting and quantifying minute quantities of the herbicide, atrazine, left in biological samples, such antibody having been made by the process of first substituting a soluble straight chain amino acid at the 4 or 6 position of 2-chloro-4(isopropylamino)-6-(ethylamino)-S- atrazine so as to leave the chlorine exposed at the 2 position along with one of the amino groups, then conjugating the resulting substitution pro- duct at the site of the amino acid group to a lysine-rich protein, using the mixed anhydride method in a strongly-basic aqueous solution, then inoculating a susceptible host with the anti- gen to evoke an immune response, and thereafter harvesting the antibody.

4530900

S O L U B L E I N S O L U B L E P O L Y M E R S IN

E N Z Y M E I M M U N O A S S A Y

David L Marshall assigned to Seragen Dia- gnostics Inc

The invention pertains to an improved hetero- geneous enzymeimmunoassay involving the use of a reversibly soluble polymeric substance ac- ting as the support for the antibody, in the direct method the antigen to be detected and an enzyme labeled antigen are bound by antibody which is chemically linked to the soluble polymeric sub- stance. The polymer is rendered insoluble and removed from the test solution. After resolubilization into a solution containing subs- trate for the enzyme label, the assay for antigen is completed by determination of enzymatic ac- tivity. In the indirect method, the antigen to be detected and an enzyme labeled antigen are in- cubated with a primary antibody unattached to the polymeric substance. After addition of a second antibody which is chemically linked to the polymeric substance, the polymer is rendered insoluble and the assay is performed as in the direct method described above.

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4530907

P E R F U S I O N - C U L T I V A T I O N O F A N I M A L C E L L S A N D

E Q U I P M E N T T H E R E F O R

Anders Peterson, Erik Walum, 150 11 Bj Sweded

The invention aims at replacing animal experi- ments for determining acute toxicity (LD50 values) by utilizing stable perfusion-cultivated cell cultures and also at producing a cultivation chamber which is movable during operation, for determining various parameters.

4532038

F L O W C O N T R O L A P P A R A T U S F O R A E R O B I C S E W A G E

T R E A T M E N T

John H Reid

In aerobic wastewater treatment systems, such as sewage treatment, liquid in a channel spanned by a liquid-tight barrier is moved past the barrier by a liquid-gas contact pump, thus dissolving a gas in the liquid and creating a differential head that provides flow energy for moving the liquid in plug-type flow through the channel, which is preferably a continuous-flow pipeline or a circuit-flow oxidation ditch, without energy- wasteful vertical circulation. The barrier further provides a structure for mounting submerged static aerators or a draft tube, in combination with a surface aerator, such as a submerged tur- bine or surface-disposed impeller aerator, as the liquid-gas contact pump. The barrier is disposed at sufficient depth, when combined with static aerators or when telescopically attached to the draft tube of a floating surface aerator, to enable the liquid level to be selectively varied so that flow equalization to an oxidation ditch and wastewater storage therewithin are provided. Because of the plug-type flow and capability of maintaining it at 0.75-1.25 feet per second, a grit settling capability can be combined with aerobic digestive treatment within the oxidation ditch by placing a grit settling system slightly past the raw sewage inlet in a channel of the oxidation ditch. By employing a plurality of oxidation ditches, of which one is sequential used for clarification, and by incorporating a grit settling system within each of the oxidation ditches, the entire wastewater output from a large municipality, slaughterhouse, or poultry processing plant, for example, can be processed without preliminary or supplementary treatment in any other ap- paratus.