PATENT ABSTRACTS

4670379

P O L Y N U C L E O T I D E H Y D R I D I Z A T I O N A S S A Y S E M P L O Y I N G C A T A L Y Z E D

L U M I N E S C E N C E

Jeffrey A Miller assigned to E 1 Du Pont de Nemours and Company

Polynucleotide hydridization assays employing catalyzed luminescence.

307

cytoplasmic antigens find application in the immunodiagnosis of Candida infections. + R E + P A A procedure is provided for pre- paring partially purified cytoplasmic antigen of pathogenic Candida species for administration to splenocyte-donating mice. Also provided is a method for the biochemical purification of cyto- plasmic antigen of a pathogenic Candida species used for the preparation of monoclonal and monospecific, polyclonal antisera thereto.

4670380

A S S A Y S U T I L I Z I N G L A B E L E D N U C L E I C A C I D P R O B E S

4670388

M E T H O D O F I N C O R P O R A T I N G D N A I N T O G E N O M E O F

D R O S O P H I L A

Nanibhushan Dattagupta assigned to Molecular Diagnostics Inc

In a method for determining a particular poly- nucleotide sequence in a test medium containing single stranded nucleic acids wherein the sample is subjected to a hybridization reaction with a labeled detection probe having a substantially complementary polynucleotide sequence, and wherein after hybridization the label in said probe is assayed, the improvement wherein the label in said labeled probe comprises a fluores- cent nucleotide which is linked by a phosphate ester linkage to said probe. Probes and kits there- for are also provided.

4670382

M O N O C L O N A L A N T I B O D Y T O C A N D I D A A L B I C A N S

C Y T O P L A S M I C A N T I G E N S A N D M E T H O D S O F P R E P A R I N G S A M E

Gerald Rubin, Allan C Spradling assigned to Carnegie Institution of Washington

A method for incorporating a desired DNA sequence into the genome of a recipient multi- cellular organism comprising: (1) producing a transposable element of DNA, the element com- prising a defined sequence of nucleotide base pairs wherein the defined sequence of nucleotide base pairs comprises at least two sets of target DNA sequences recognizable by transposase and a fragment of DNA, encoding for a struc- tural gene, regulatory gene or other functional DNA, inserted between the sets of target DNA sequences; (2) incorporating the transposable element produced in (1) above into a cell of a recipient multi-cellular organism; and (3) causing the transposable element to be affirma- tively inserted into the genome of the recipient multi-cellular organism. The transposable ele- ment can additionally become a part of the heritable genome of the recipient multi-cellular organism.

Helen Buckley, Michael T Largen, Nancy A Strockbine assigned to Temple University£13 of the Commonwealth System of Higher Education

Two novel hybridoma cell lines, ATCC £190 HB-8397 and ATCC + 190 HB-8398 produce monoclonal antibody monospecific to a single determinant shared by a set of three closely related cytoplasmic antigens of + 1 Candida al- bicans. + L The antigens have molecular weights of 120+14 135Kd, 48+1452 Kd. and 35+ 14 38 Kd. The hybridomas are formed by fusing splenocytes from immunized BALB/c mice with SP2/O-Ag 14 myeloma cells. Monoclonal and monospecific, polyclonal antibodies to these

4670389

P R O C E S S F O R P R O D U C I N G N - A C E T Y L N E U R A M I N A T E L Y A S E

Takayuk Uwajima, Kazuo Aisaka, Machida, Japan assigned to Kyowa Hakko Kogyo Co Ltd

The present invention provides a process for producing N-acetylneuraminate lyase using a microorganism constructed by recombinant DNA techniques.