250 PATENT ABSTRACTS

4708929 4710386

M E T H O D S F O R P R O T E I N B I N D I N G E N Z Y M E

C O M P L E M E N T A T I O N A S S A Y S

Daniel R Henderson assigned to Microgenics Corporation

This invention relates to improved methods and novel compositions for enzyme complementa- tion assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of beta-galactosidase is described. Both homogeneous and hetero- geneous assays utilizing these polypeptides are described.

4708934

A L L N A T U R A L , R E A D Y - T O - E A T E N Z Y M E - S A C C H A R I F I E D C E R E A L D E R I V E D F R O M W H O L E C E R E A L G R A I N

Charles V Fulger, Ernest Gum assigned to Gene- ral Foods Corporation

A process for producing an all natural, enzyme- saccharified, cereal derived from whole grain is disclosed. The process involves milling and separating the whole grain to produce a bran fraction, an endosperm fraction, and a germ fraction. The germ fraction is processed by toasting and grinding and preferably by removing bran before the toasting. The bran fraction and any bran material separated out from the ground germ is modified to increase its functionality by high temperature, high shear ex- trusion in a counter-rotating twin screw ex- truder. The endosperm fraction is coarsely milled, made into a slurry, cooked and then en- zymatically hydrolyzed. The final step involves recombining the processed fractions, to form a cereal dough which is further processed to pro- duce a ready-to-eat breakfast cereal. Optionally from 1-15% of the whole grain may be malted and added into the cereal dough.

A L P H A - A M I D A T I O N E N Z Y M E

James P Gilligan, Barry N Jones assigned to Unigene Laboratories Inc

Peptidyl-glycine alpha-amidating mono- oxygenase is an enzyme extractable from medul- lary thyroid carcinoma cell lines and tissue samples, having a molecular mass of about 60,000 to 65,000 daltons. It has been purified so as to exhibit a single, homogeneous, well-defined band using electrophoretic procedures perfor- med on SDS-polyacrylamide gels, and has a specific enzymatic activity of at least 50 mU per mg protein. The free or immobilized enzyme, in the presence of Cu+ 2 ions, ascorbate, and oxy- gen, can be used to prepare an alpha-amidated protein from a polypeptide substrate possessing a carboxyl-terminal glycine residue. The purified enzyme can be used as an antigen in order to pro- duce enzyme-specific monoclonal antibodies, and can provide the information necessary to design and construct prokaryotes or other ap- propriate unicellular organisms or host cells isolated from multicellular organisms which possess peptidyl-glycine alpha-amidating cap- ability.

4711739

E N Z Y M E P R E S P O T T E R C O M P O S I T I O N S T A B I L I Z E D

W I T H W A T E R I N S O L U B L E P O L Y E S T E R O R P O L Y E T H E R

P O L Y O L

Thomas V Kandathil assigned to S C Johnson & Son Inc

The present invention relates to water-in-oil em- ulsion prespotter laundry compositions. More particularly, the invention relates to water-in-oil emulsion prespotter laundry compositions con- taining enzymes and specific polyester or poly- ether polyols, which immobilize the enzymes to prevent their degradation during prolonged storage.