PATENT ABSTRACTS 381

variable, joining, constant, transmembran¢, and probe to diagnose the aggressiveness of a car- cytoplasmic regions. The structure, amino acid, cinoma or the effectiveness of an agent for and nucleotide sequence of the lymphocyte treating cancer cells. receptor suhunit were determined using cDNA clones derived from a functional murine cyto- toxic T lymphocyte clone. The genes correspon- 4862360 ding to these cDNA are expressed and rearranged specifically in T cells and have S I G N A L P R O C E S S I N G M E T H O D significant sequence homologies to immuno- I N A U T O R A D I O G R A P H Y globulin V and C genes. T cell receptor subunits may be produced from the cDNA clones. The Tsutomu Kimura, Kazuhir Hishinuma, protein molecules may be further used for the Kanagawa, Japan assigned to Fuji Photo Film production of T-cell clone specific antibodies. Co Ltd

A signal processing method in autoradiography 4874853 for determines of the base sequence of DNA or a

DNA fragment, by employing groups of S Y N T H E T I C radioactively labeled base specific cleavage pro-

O L I G O N U C L E O T I D E S U S E F U L IN ducts or mixtures thereof obtained by D I A G N O S I S O F C H R O N I C specifically cleaving the DNA or DNA fragment

M Y E L O G E N O U S L E U K E M I A labeled with a radioactive element and resolved one-dimensionally in parallel relation to each other to form resolved rows on a support

John J Rossi assigned to City of Hope medium. An autograph is obtained having Ioca- tional information on groups of radioactively

Synthetic oligonucleotide for detection of the labeled cleavage products contained in the rows bcr-abl RNA from blood or bone marrow of pat- on the support medium. An electrical digital ients with chronic myelogenous leukemia, signal corresponding to the autoradiograph is

then generated. Sampling points in each resolved row of the digital signal are next detected.

4861708 Reference sampling points are then determined in a plurality of reference rows which are either

R E S T R I C T I O N F R A G M E N T directly provided on the support medium and/or A N A L Y S I S O F I N D I V I D U A L S synthesized from certain resolved rows. Cor-

U S I N G C A R D I O V A S C U L A R responding reference sampling points in the plural reference rows are joined to generate

S Y S T E M P R O B E S plural continuous lines comprising straight lines, polygonal lines or curved lines. Sampling points

Philippe M Frossard assigned to Biotechnology in the remaining non-reference rows are iden- Research Partners Ltd tiffed by comparison with the continuous lines to

thus determine the base sequence of the DNA or Polymorphisms in the renin, kallikrein, and DNA fragment. ANF gene regions are predictive for hyperten- sion in individual human subjects.

4863847

4861710 P R O C E S S F O R D I R E C T L Y D E T E R M I N I N G A P U R I N I C A N D

R E C O M B I N A N T D N A C L O N E A P Y R I M I D I N I C S I T E S IN D N A E N C O D I N G L A M I N I N R E C E P T O R

Myria Talpaert-Borle, Miche Liuzzi, Ispra, Italy Mark E Sobel, Lance A Liotta, Ulla Wewer, assigned to Europaische Atomgemeinschaft Michael C Jaye, William N Drohan assigned to The United States of America as represented by A simple and fast process is described which ser- the Department of Health and Human Services yes to directly determine apurinic and

apyrimidinic sites (AP sites) in DNA using The invention provides a recombinant cDNA (14C)methoxyamine which, after loss ofa purine clone encoding cell surface receptor for laminin, or pyrimidine base, respectively, reacts with the as well as a probe and methods of using that resultant aldehyde groups of the deoxyribose

382 PATENT ABSTRACTS

groups. The incorporation of the (14C)methox- 4863857 yamine in DNA is proportional to the number of AP sites. Since methoxyamine does not cause the P O L Y P E P T I D E DNA to degrade, the unreacted AP sites can be C O M P L E M E N T A R Y T O measured in order to determine the radioactivity P E P T I D E S O R P R O T E I N S of the acid-insoluble fraction. The process lends H A V I N G A N A M I N O A C I D itself to the analysis of DNA-damages such as S E Q U E N C E O R N U C L E O T I D E those which are physically or chemically pro- duced. Moreover it is suitable to demonstrate C O D I N G S E Q U E N C E A T L E A S T and determine the activity ofDNA glycosylases, P A R T I A L L Y K N O W N in particular of uracil-DNA glycosylases.

J Edwin Blalock, Eric M Smith, Kenneth L Bost assigned to Board of Regents The University of Texas System

A method for determining the amino acid 4863848 sequence of a polypeptide complementary to at

least a portion of an original peptide or protein. In one aspect the method involves: (a) deter-

D O U B L E - S T R A N D E D V E C T O R mining a first nucleotide sequence of a first A N D P R O C E S S U S I N G I T nucleic acid coding for the biosynthesis of at

least a portion of the original peptide or protein; Helmut Blocker, Ronald Frank, Guido Vol- (b) ascertaining a second nucleotide sequence of ckaert, Hamburg, Federal Republic Of Ger- a second nucleic acid which base-pairs with the many assigned to Gesellschaft fur first nucleotide sequence of the first nucleic acid, Biotechnologische Forschung mbH (GBF) the first and second nucleic acids pairing in anti-

parallel directions; and (c) determining the The invention concerns a double-stranded aminoacidsequenceofthecomplementarypoly- recombinant DNA cloning vector comprising peptide by the second nucleotide sequence when one single insertion site for foreign DNA and read in the same reading frame as the first one or two labelling sites adjacent or approx- nucleotide sequence. The complementary poly- imate to said insertion site. In the instance of two peptide whose amino acid sequence is thus deter- labelling sites, said sites flank the insertion site. mined may be obtained by diverse means such The labelling site(s) can be individually labelled as, for example, chemical synthesis, derivation in a fashion that results in the labelling of the 3' from a protein or larger polypeptide containing end of either strand. The use of the invention in a said amino acid sequence, or, when the second process affords the ability to individually label nucleic acid is DNA, inserting the second only one strand at the 3' end in a simplified nucleotide sequence into a plasmid to form a method for base sequencing analysis, recombinant DNA plasmid vector and trans-

forming a unicellular organism therewith to pro- duce a transformant unicellular organism biosynthesizing said complementary poly- peptide. The ascertainment of particular nucleotide sequences may be circumvented, in

4863849 one aspect, by utilizing the relationships of amino acids having complementary hydro- pathies for substitutions as generally dictated by

A U T O M A T A B L E P R O C E S S F O R base-pairing nucleotide complementarity. S E Q U E N C I N G N U C L E O T I D E

Robert J Melamede assigned to New York 4865967

Medical College A U T O R A D I O G R A P H I C G E N E -

This invention relates to a method for deter- S C R E E N I N G M E T H O D mining the nucleotide sequence of DNA an RNA molecules. The method is automatable Hisashi Shiraishi, Junji Miyahara, Hisatoyo and avoids the use of radioactive labels and gel Kato, Minami Ashigara, Japan assigned to Fuji electrophoresis. The method is also adaptable Photo Film Co Ltd for introducing site-specific mutations in DNA and RNA molecules. An autoradiographic gene-screening method