4966765 novel coleopteran-active bacillus thuringiensis isolate
TRANSCRIPT
82 PATENT ABSTRACTS
cellular protease production may also be used. C. glutamicum is used as a model organism for the secretion system. This Gram positive, non- pathogenic bacteria can efficiently utilize hetero- logous expression and secretion signals originating from a variety of both Gram nega- tive and Gram positive bacteria to provide the basis for the overproduction and secretion of cloned gene products in a given Corynebac- terium host, as demonstrated by the expression and secretion by C. glutamicum of a lipase encoded by a gene from S. hyicus and a thermo- nuclease encoded by a gene from S. aureus.
4965199
P R E P A R A T I O N O F F U N C T I O N A L H U M A N F A C T O R VII I IN
M A M M A L I A N C E L L S U S I N G M E T H O T R E X A T E B A S E D
S E L E C T I O N
Daniel J Capon, Richard M Lawn, Arthur D Levinson, Gordon A Vehar, William I Wood as- signed to Genentech lnc
A method for producing factor VIII in recom- binant mammalian host cells utilizing an expres- sion vector containing a selectable marker DNA and an amplifiable marker DNA. The initial selection is based upon the selectable marker and subsequent amplification of factor VIII DNA and amplifiable marker DNA is conducted in cells not deficient in the amplifiable marker.
4965207
C O L O R M A R K E R F O R C L O N I N G S I N S T R E P T O M Y C E S
L I V I D A N S
Gunter Muth, Wolfgang Wohlleben, Alfred Puhler, Gerhard Wohner, Rudiger Marquardt, Bielefeld, Federal Republic Of Germany as- signed to Hoechst Aktiengesellschaft
Intensely crimson colonies are obtained from Streptomyces coelicolor DSM 3030 by total digestion of the total DNA, cloning into a suitable vector, and transformation of Strep- tomyces lividans. Re-isolation of the plasmid DNA and cutting with BamHI results in frag- ments about 3.4 to 9 kb in length, on which is located the gene which determines production of the coloring agent. This gene is suitable as mar- ker, in particular as inactivation marker, in Streptomycetes.
4966765
N O V E L C O L E O P T E R A N - A C T I V E B A C I L L U S T H U R I N G I E N S I S
I S O L A T E
Jewel Payne, Michelle Burrascano, George G Soares assigned to Mycogen Corporation
The subject invention concerns a novel and use- ful insecticide with activity against insect pests of the order Coleoptera. Pests in the order Coleop- tera do heavy damage to crops, e.g., corn. The insecticide of the subject invention is a novel B. thuringiensis microbe referred to as B.t. PS86B 1, or mutants thereof. The spores or crystals of this microbe are useful to control coleopteran pests in various environments.
4966792
M E T H O D O F P R O D U C I N G G R A D I E N T G E L M E D I U M
M E M B R A N E F O R E L E C T R O P H O R E S I S
Fumitak Terai, Kimio Yukawa, Mineo Suefuji, Kanagawa, Japan assigned to Fuji Photo Film Co Ltd
A method for producing gradient gel medium membrane for electrophoresis for determining the base sequence of D N A or DNA partially decomposed material providing an improved productivity. High and low concentration monomer solutions are mixed with a pre- determined quantity ofpolymerizing reaction in- itiator solution by a static mixer to prepare a gel forming solution for coating on a continuously moving web. The flow-rate ratio of the high and low concentration monomer solutions is gradually changed so as to vary the concentra- tion of the monomer in the gel forming solution alternatively from low to high and from high to low along the web.
4966837
M E T H O D S A N D C O M P O S I T I O N S F O R T H E D E T E C T I O N O F
F A M I L I A L H Y P E R C H O L E S T E R O L E M I A
Michael S Brown, Joseph Goldstein, David W Russell assigned to Board of Regents The University of Texas System