268

4983522

PATENT ABSTRACTS

M E T H O D F O R P R O D U C I N G T H E H I N P I R E S T R I C T I O N

E N D O N U C L E A S E A N D M E T H Y L A S E

Janet Barsomian, Geoffrey Wilson assigned to New England Biolabs Inc

The present invention is directed to a method for cloning and producing the HinPI restriction endonuclease by (1) introducing the restriction endonuclease gene from Haemophilus influenza P1 into a host whereby the restriction gene is ex- pressed; (2) fermenting the host which contains the vector encoding and expressing the HinPI restriction endonuclease, and (3) purifying the HinPI restriction endonuclease from the fermen- ted host which contains the vector encoding and expressing the HinPl restriction endonuclease activity.

4983525

P L A S M I D S D E R I V E D F R O M A C T I N O M A D U R A S P E C I E S

Jerald S Feitelson, William Maiese assigned to American Cyanamid Company

Plasmid DNA was isolated from two Ac- tinomadura species and characterized by map- ping the sites of restriction endonuclease cleavages and by calculation of total molecular size.

4983542

M E T H O D F O R P R O D U C I N G T H E X B A I R E S T R I C T I O N

E N D O N U C L E A S E A N D M E T H Y L A S E

Elizabeth M Van Cott, Geoffrey G Wilson as- signed to New England Biolabs Inc

The present invention is directed to a method for cloning and producing the XbaI restriction endonuclease by (1) introducing the restriction endonuclease gene from Xanthomonas badrii into a host whereby the restriction gene is ex-

pressed; (2) fermenting the host which contains the vector encoding and expressing the Xbal restriction endonuclease, and (3) purifying the XbaI restriction endonuclease from the fermen- ted host which contains the vector encoding and expressing the XbaI restriction endonuclease ac- tivity.

4983727

P A L L A D I U M M E D I A T E D R E A C T I O N O F O R G A N I C

D I S U L F I D E S W I T H M E R C U R A T E D N U C L E I C A C I D

C O M P O N E N T S

Donald E Bergstrom, Jeffrey Jenson assigned to University of North Dakota School of Engineering & Mines Foundation

A method of preparing C-5 substituted nucleotides by reacting a C-5 position mer- curated nucleoside with an alkyl or aryl disulfide in the presence of palladium(II) ion. The resul- ting new method for attaching linker arms to nucleic acids (DNA,RNA) provides a wide ranging reaction far more useful than those heretofore developed, and allows preparation of modified components with linking reactive func- tionality that allows usefulness as probes, therapeutics, and for DNA sequencing.

4983728

N U C L E I C A C I D P R O B E S O F H U M A N P A P I L L O M A V I R U S

Alber Herzog, Alfred Cravador, Sophie Houard, Alex Bollen, Eppegem, Belgium as- signed to Ire-Celltarg S A

The present invention relates to probes of nucleic acids useful for detecting indifferently the various types of human papilloma virus, par- ticularly HPVIa. HPV5, HPV6b, HPV8, HPV11, HPV16, HPVI8 and HPV33, especially a probe comprising a labelled sequence of nucleic acids, characterized in that it comprises the oligomer of twelve nucleotides X-A-A-A-A-C- G-A-A-A-G-X, with X = T or U, or its comple- ment by interchanging A and X on the one hand,