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PROCESS BALANCE 3 Process Reaction

Theoretical Product Reaction (PDO Case)

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Conservation Principle

Theoretical PDO Reaction

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Example (without cell growth)

Calculate the stoichiometric coefficients a, b and c.

a C6H12O6 b CO2 + c H2O + 1 C4H10O2

Reaction Process with Cell Growth

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Cell Composition for Escherichia coli

Elemental Formula of Some Microbes

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Stoichiometry (in different way)

Example

Production of single-cell protein from hexadecane is

described by the following reaction equation:

where CH1.66O0.27N0.20 represents the biomass. If RQ=

0.43, determine the stoichiometric coefficients.

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Uptake and Secretion Rate

Process Reaction Concept:

Basis for Process Design

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What Determine q-rates?

qi= function of ( Cs, CO2, CCO2, CNH4, Cvitamin, Ctrace metal )

Still Complicated???

Single Nutrient Limiting Nutrient Medium

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Hyperbolic qs function

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Intracellular pseudo steady state

BLACK

BOX

Example

Suatu mikroorganisme untuk dapat tumbuh memerlukan

vitamin, dimana Kvit=10 nmol/L. Setelah diukur, setiap gram

mikroorganisme memuat vitamin sebanyak 20 nmol.

Seorang peneliti ingin mengkultivasi mikroorganisme

tersebut dari 2 gr/L menjadi 10gr/L dalam suatu reaktor

batch. Berapakah jumlah vitamin yang harus ditambahkan

diawal proses?

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Fermenter Technology

Next class.