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Introducing grafted cucurbits to modern agriculture: The Israeli experience Ron Cohen Section of Cucurbit Research Section of Cucurbit Research Newe Newe Ya’ar Ya’ar Research Center, Research Center, Israel Israel

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Page 1: 5 Ron Cohen Thailand Compatibility Mode

Introducing grafted cucurbitsto modern agriculture:The Israeli experience

Ron Cohen

Section of Cucurbit ResearchSection of Cucurbit Research

Newe Newe Ya’arYa’ar Research Center, Research Center,

IsraelIsrael

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What is grafting ?

Uniting of two living plant parts so that they grow as a single plant

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Methyl bromideMethyl bromide

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Grafted vegetables in Israel

Tomato Cucurbits

Melon WatermelonCucumber

Soil-borne diseases – Fusarium, Mpnosporascus,MacrophominaMacrophomina

Response to insects and nematodesSalinity and marginal water

Rootstock breeding

Agro-technology and yield – Fruit qualityRootstock- scion compatibility

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Soil-borne diseases

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Grafted Non grafted

GraftedNon-grafted

Watermelon Vine DeclineWestern Galilee

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Macrophomina phaseolina

Monosporascus cannonballus

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Newe Ya’ar Research Center

Macrophomina land

Northern Valleys

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Is it profitable growing grafted melons in mid-summerfor the local market ?

Transplants Prices:

Non-grafted : 0.35 NIS

Grafted : 2.5 NIS

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Above GraftDistant from Graft

Mixture of Fusarium sp. and

Macrophomona phaseolina

Root Scion

שדה יעקב–מלון מורכב על כנת דלעת 2006ספטמבר

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Sdee Yaacov 2006

Yie

ld (

kg/D

)

a

aa

a

a

aa

b

6405 Eyal

Stand (cm)

Yie

ld (

kg/D

)

b

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Monosporascus land

Jordan“Zohar” experiment station

‘Ein tamar

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Melon physiological collapse at ‘En TamarSpring, 2000

35404550

Co

lla

pse

in

cid

ence

TRELLISED

PROSTRATE

05

1015202530

Co

lla

pse

in

cid

ence

(%)

non grafted melon-melon melon-Cucurbita

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-Melon grafted onto rootstocks from Newe Ya’ar breeding program (#18 and 34) did not collapse when grown trellised.

-Melon yield was 60% higher as compared to non-grafted melons.

-The yield was 30% significantly higher compared to melon plants grafted onto the currently used commercial rootstock.

60

Accumulative yield - saline water (EC 4.2)

0

10

20

30

40

50

60

90 110 130 150 170 190

Yie

ld (

Kg

/plo

t)

Days from planting

18

34

TZ-148

control

A

AB

BC

C

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The two step selection

Spring

Adapting grafted melons to trellised cultivation

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Non-grafted

Second Fruit Wave First Fruit Wave

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(

Grafted

Second Fruit WaveFirst Fruit Wave

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Stress conditions

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21.2.2002

Boron 10 mg/l

13.3.2002

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Boron absorption

Non-grafted

y = 2.6252e

R2 = 0.9338

60.00

70.00

80.00

90.00

Grafted

y = 2.8602e

R2 = 0.8038

60.00

70.00

80.00

90.00

B in

leav

es (

mg

/l)

0.00

10.00

20.00

30.00

40.00

50.00

60.00

0.25 0.8 2.5 5 10

B in irrigation water (mg/l)

B in

leav

es

0.00

10.00

20.00

30.00

40.00

50.00

60.00

0.25 0.8 2.5 5 10

B in irrigation water (mg/l)

B in

leav

es (

mg

/l)

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Fruit quality

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Mini- watermelon

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Incompatibility,

water uptakewater uptake

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Arava non-grafted

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Arava / RS 60

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Physiological

incompatibility

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Non grafted

Arava Valley summer 2003

Grafted

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Anatomical Examination of Grafting Site

62/ערבה 11 days post grafting 14 days post grafting

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Effect of TIBA (200 ppm) on the development of

Arava melon grafted on the Cucurbita rootstock RS 62

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IAA

IAA

Compatible Incompatible

Root growth

Ethylene induction

ROS production

Root death

Ethylene induction

ROS production

Antioxidation

system

genes ?

genes ?

genes ?

*

*

*

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