448 PATENT ABSTRACTS

A method and reagents are provided for deter- mining whether a human cell is a hemopoietic cell and whether a human tissue cell is in a neoplastic state. Human cells which express only leukocyte-plastin (I-plastin) are hemopoietic cells and human cells which express both I- plastin and tissue-plastin (t-plastin) are neoplastic. The method can be performed using isoform-specific plastin nucleotide probes or isoform-specific antiplastin antibodies.

5OO2873

D N A S E Q U E N C E E N C O D I N G A L Y M P H O C Y T E A D H E S I O N

R E C E P T O R F O R H I G H E N D O T H E L I U M

John Thomas St, W Michael Gallatin, Rejean L Idzerda assigned to Fred Hutchinson Cancer Research Center

A lymphoid cell line eDNA that encodes an ad- hesion receptor for high endothelial venules (HEV).

5002874

G E N E T I C A L L Y E N G I N E E R E D E U C A R Y O T I C H O S T C E L L S C A P A B L E O F E X P R E S S I N G

M O D I F I E D F O R M S O F EIF-2 A L P H A

Randal J Kaufman assigned to Genetics In- stitute Inc

Eucaryotic host cells are disclosed which contain a DNA molecule encoding an elF-2 alpha mutant and preferably a DNA sequence encoding a desired heterologous protein. The DNA sequences are linked to expression control sequences permitting expression of the mutant elF-2 alpha gene and the heterologous gene. Cul- turing such cells provides a method for the pro- duction of the desired heterologous protein. The mutations eliminate one or both serine residues at positions 48 and 51 of the elF-2 sequence. In another aspect of the invention, the elF-2 5"- untranslated sequence was observed to have ef- fects on translation of heterologous mRNAs.

5002875

P L A S I M I D S C O N T A I N I N G T H E G E N E F O R D N A P O L Y M E R A S E I

F R O M S T R E P T O C O C C U S P N E U M O N I A E

Sanford A Lacks, Susana Martinez, Paloma Lopez, Manuel Espinosa assigned to The United States of America as represented by the United States Department of Energy

A method is disclosed for cloning the gene which encodes a DNA polymerase-exonuclease of Streptococcus pneumoniae. Plasmid pSM22, the vector containing the pneumocccal polA gene, facilitates the expression of 50-fold greater amounts of the Poll enzyme.

5002876

Y E A S T P R O D U C T I O N O F H U M A N T U M O R N E C R O S I S

F A C T O R

Kotikanyadan Sreekrishna, Motohiro Euke, Rica H Potenz assigned to Phillips Petroleum Company

Novel DNA constructs comprising yeast regulatory regions plus the structural coding re- gion for human tumor necrosis factor, are dis- closed. These novel constructs are incorporated into a variety of linear and circular plasmids. Such plasmids are used for yeast transformation and ultimately for the production of human tumor necrosis factor by yeast.

5002882

M E T H O D F O R P R O D U C I N G T H E X M A ! R E S T R I C T I O N

E N D O N U C L E A S E A N D M E T H Y L A S E

Keith D Lunnen, Geoffrey G Wilson assigned to New England Biolabs Inc

The present invention is directed to a method for cloning and producing the Xmal restriction endonuclease by (1) introducing the restriction endonuclease gene from X. malvacaerum into a host whereby the restriction gene is expressed: (2) fermenting the host which contains the plas- mid encoding and expressing the Xmal restric- tion endonuclease activity, and (3) purifying the Xmal restriction endonuclease from the fermen- ted host which contains the plasmid encoding and expressing the Xmal restriction endo- nuclease activity.

5O02888

M U T A N T M I C R O O R G A N I S M S U S E F U L F O R CLEAVAGE O F

O R G A N I C C-S B O N D S

John J Kilbane assigned to Institute of Gas Technology