PATENT ABSTRACTS

5013556

L I P O S O M E S W I T H E N H A N C E D C I R C U L A T I O N T I M E

Martin Woodle, Francis Martin, Annie Yau- Young, Carl Redemann assigned to Liposome Technology Inc

A liposome composition which contains bet- ween 1-20 mole percent of an amphipathic lipid derivatized with a polyalkylether, as exemplified by phosphatidylethanolamine derivatized with polyethyleneglycol. The derivatized lipid enchances the circulation time of the liposomes severalfold, and this enhancement is achieved with either fluid or membrane-rigidifying lipo- some components. Also disclosed are methods for delivering a drug for slow release from the bloodstream, and for targeting a selected tissue or cells with liposomes, via the bloodstream.

5013644

I D E N T I F I C A T I O N O F A F R I C A N I Z E D H O N E Y B E E S

David Severson, Judd Aiken assigned to Wisconsin Alumni Research Foundation

Africanized honey bees are identified by using a DNA probe capable of identifying a specific repeating gene sequence in the honey bee DNA which is a diagnostic characteristic of African honey bees when located on a defined restriction endonuclease fragment from bee genomic DNA. When the DNA probe is applied to an elec- trophoresis gel on which the restriction frag- ments are resolved, the probe hybridizes to the diagnostic fragment, if present. The hybridiza- tion to the repeating sequence can then be detec- ted by detection of the marker.

5 0 1 3 ~ 5

I M M U N O L O G I C A L M E T H O D S A N D M A T E R I A L S F O R

D E T E C T I O N O F T U M O R A S S O C I A T E D A N T I G E N S

Yung D Kim assigned to Abbott Llboratories

Disclosed are immunological methods and materials for detection of antigens associated with breast or prostate cancer disease states. Pre- sently preferred antibody preparations (e.g.,

515

PR92 monoclonal antibodies produced by hybridoma cell line ATCC HB 9390) are em- ployed in immunoassays performed on patient body fluids and for purification of tumor- associated antigen compositions.

5 0 1 ~ 5 5

P R O C E S S F O R T H E P R E P A R A T I O N O F C O L O R A N T S

O R M O N A C O L I N K IN A S T E R I L E F L U I D I Z E D B E D O F L O W W A T E R

C O N T E N T

Thomas Bayer. Rainer Buchholz, Hans-Matthia Deger, Joachim Wink, Hanover, Federal Republic Of Germany assigned to Hoechst Ak- tiengesellschaft

The invention relates to a process for the pre- paration of colorants and/or active compounds by culturing a microorganism of the genus Monaseus in a sterilizable fluidized-bed fermen- ter of low water content, such as was proposed in German Patent Application P 3,625,698.6. The fluidizable particle used here is the micro- organism itself-in pellet form or grown on carriers-which can be supplied with nutrients by spraying in substrate solutions. The colorants and/or active compounds are isolated by extrac- tion with organic solvents.

5013661

L I P O P O L Y S A C C H A R I D E - S P E C I F I C A C Y L O X Y A C Y L

H Y D R O L A S E

Robert S Munford. Catherine L Hall assigned to The Board of Regents The University of Texas System

An acyloxyacyl hydrolase from the human pro- myelocyte cell line HL-60 has been found to specifically hydrolyze fatty acids from their ester linkages to hydroxy groups of 3-hydroxyfatty acids, the latter being being bound in turn to lipopolysaccharide glycosaminyl residues. The hydrolyzed fatty acids may include dodecanoic acid, tetradecanoic acid and hexadecanoic acid. This enzyme showed a molecular weight by gel exclusion chromatography between about 50,000 Daltons and about 70,000 Daltons, and a molecular weight by polyacrylamide gel elec- trophoresis with sodium dodecylsulphate, using reduced molecular weight standards, of approx-