PATENT ABSTRACTS 465

A polynucleotide molecule expressible in a given host comprising the sequence of the arab pro- moter operably linked to a gene which is hetero- Iogous to said host. The heterologous gene codes for a peptide that is biologically active. The in- vention also relates to a genetic construct which comprises a first genetic sequence coding for cecropin operably linked to a second genetic sequence coding for a polypeptide which is capable of suppressing the bactericidal effect of the resulting fusion protein towards an otherwise cecropin sensitive bacterium.

5028531

5028534

D N A C L O N E S O F H U M A N P L A C E N T A L P L A S M I N O G E N

A C T I V A T O R I N H I B I T O R

J Evan Sadler, Tze-Chein Wun assigned to Washington Univ; Monsanto Compa

cDNA clones having a base sequence for human placental plasminogen activator inhibitor (PAl- 2) have been developed and characterized and the amino acid sequence of the PAI-2 has been determined. The PAl-2 protein has then been ex- pressed in prokaryotic and eukaryotic cells.

I G F - I F U S I O N P R O T E I N S ; P R O T E C T I O N O F I G F - I F R O M

D E G R A D A T I O N B Y H O S T C E L L ; A N D P R O C E S S E S F O R T H E

P R O D U C T I O N T H E R E O F

Ikuo Ueda, Mineo Niwa, Yoshimasa Saitoh, Susumu Satoh, Chihiro Kusunoki, Tadashi Kitaguchi, Hiroki Ono, Toyonaka, Japan as- signed to Fujisawa Pharmaceutical Company Ltd

IGF-I fused with a protective peptide, in which the protective peptide is a protein peptide and is used for the protection of IGF-I from degrada- tion by protease in cells of E. coil is disclosed. Also disclosed are genes coding for the fused IGF-I's, plasmids containing the genes, and E. coli microorganisms transformedwith the plas- mids.

5028539

E T H A N O L P R O D U C T I O N U S I N G E N G I N E E R E D M U T A N T E. C O L I

Lonnie O Ingrain, David Clark assigned to The University of Florida

The subject invention concerns novel means and materials for producing ethanol as a fermenta- tion product. Mutant E. coil are transformed with a gene coding for pyruvate decarboxylase activity. The resulting system is capable of pro- ducing relatively large amounts of ethanol from a variety of biomass sources.

5028540

A V I A N I M M U N O G L O B U L I N P R O D U C I N G C E L L L I N E S

5028533

P R E C U R S O R P O L Y P E P T I D E , D N A S E Q U E N C E C O D I N G

T H E R E F O R , V E C T O R S H O S T O R G A N I S M S , A N D P R O C E S S E S

I N V O L V I N G S A M E

Roy S Tubb, Espoo, Finland assigned to Cell- Teeh Limited

A yeast expression and secretion vector is described. The vector carries a nucleotide sequence coding for a precursor polypeptide which includes the signal sequence of amylo- alpha-1-,-4-glucosidase from Saccharomyces diastaticus.

Eric H Humphries assigned to The Board of Re- gents The University of Texas System

The present invention involves a method for pre- paring antibody-producing chicken cell clones. This method comprises a series of steps including initially immunizing a first chicken with a desired antigen. A population of antibody-producing lymphocytes from bursa or spleen of the first chicken is separated. The antibody-producing lymphocyte population is then infected with helper-free reticuloendotheliosis virus REV-T or helper-free reticuloendotheliosis virus REV-T and CSV (preferably REV-T(CSV) and trans- planted into a second chicken, the second chicken having been pretreated to remove nor- mal B cells. The transplanted lymphocytes to proliferate in the second chicken, preferably for a period of at least about two weeks. The