494 PATENT ABSTRACTS

The flowcel[ also includes a chuck for receipt of a thermal probe. The probe is made of elec- tromagnetically non-interactive material. The flowcell is enclosed inside the flowcell holder. The flowcell holder includes a pair of intake ports into a cavity having an open end at the flowcell for turbulently flowing a temperature controlled gas against the flowcell. The cavity has rough walls to promote the turbulent flow. The flowcell holder includes an exhaust port for flowing the gas out of the flowcell holder which also serves as a port for another thermal probe. A one-way valve prevents flow of the liquid nutr- ient out of the chamber through the intake port. Temperature controlled air is supplied to the flowcell holder by dividing air from an air supply into two conduits. One conduit travels through a coil inside a refrigerated liquid bath and the other through a coil inside a heated liquid bath. The heated and cooled air are recombined to make an even temperature air supply which is then divided once more to provide the two air supplies for creating a turbulent supply of tem- perature controlled air through the two flowcell holder intake ports. The flowcell may include on one side a semi-permeable membrane for in- troducing a gas/liquid interface to the cellbed.

5028675

P O L Y A M I D E R E S I N A N D M E T H O D F O R P R E P A R A T I O N OF

R E A G E N T S F O R I M M U N O D I A G N O S T I C U S E

Patrick Kanda, Ronald C Kennedy, James q Sparrow assigned to Southwest Foundation for Biomedical Research; Baylor College of Medici

A polyamide resin for use in peptide and protein synthesis, and a method of preparing and using same. The polyamide resin is prepared by cross- linking a dimethylacrylamide monomer by co- polymerization with a functional monomer in an aqueous solution, emulsifying the aqueous solu- tion in an organic solvent and isolating the poly- amide resin beads formed by adding an initiator and a promoter. The beads are used as a solid phase for peptide and protein synthesis ac- cording to methods known in the art. The con- jugate of the polyamide resin and the synthesized peptide or protein is used directly for immuno- assays or immunization without the need for separation of the peptide or protein from the resin and subsequent purification.

5028545

B I O S P E C I F I C M U L T I A N A L Y T E A S S A Y M E T H O D

Erkki J Soini. Turku, Finland assigned to Wallac OY

In a biospecific multianalyte assay the use of microspheres and fluorescent labels with sub- stantially different fluorescence decay times, is combined. The assay is performed in a suspen- sion of microspheres in the form of a pool of dif- ferent microsphere categories, where the categories represent different analytes. The microspheres belonging to the respective cate- gories are first coated with a specific reactant, i.e. the microspheres function as a solid support for the reactant and for a biospecific reaction. Fluorescent labels having a short decay time are used to identify the category of each individual microsphere, while fluorescent labels having a long decay time are used to determine the con- centration of a particular analyte on the micro- sphere by means of the biospecific reaction.

5028793

I M A G I N G S C R E E N F O R E L E C T R O P H O R E S I S

A P P L I C A T I O N S

Josep Lindmayer, Charles Y Wrigley, George Storti assigned to Quantex Corporation

An imaging screen for detecting and storing in- formation corresponding to the pattern of emis- sion from an electrophoresis gel containing radioactively labelled, dye-tagged or chem- iluminescent labelled DNA, RNA. or protein fragments. The imaging screen is coated with an electron trapping material which releasably stores energy from the impingement of the emis- sion from the electrophoresis gel in the form of energy corresponding the pattern and flux of the emission. When subjected to optical energy of a tirst wavelength, the electron trapping material releases the stored energy in the form of optical energy of a second wavelength corresponding the flux and pattern of the emission from the electrophoresis gel. The released optical energy of a .second wavelength is then detected and coverted to electrical signals representative of the flux and pattern of emission from the elec- trophoresis gel.