118 PATENT ABSTRACTS

5194601

L Y T I C O R I G I N O F R E P L I C A T I O N F O R E P S T E I N - B A R R V I R U S (E B V)

William M Sugden, Wolfgan Hammerschmidt, Kanagawa, assigned to Wisconsin Alumni Research Foundation

The invention provides lymphotrophic herpes virus (preferably EBV) recombinants. The por- tions of the virus that are responsible for the packaging and lyric phase replication have been isolated and cloned. When used with vectors and hosts containing a segment that controls plasmid replication, they provide a means of carrying foreign genes into B-lymphocytes.

5196303

C L O N I N G O F D N A E N C O D I N G T R A N S C R I P T I O N F A C T O R S- I I

A N D U T I L I Z A T I O N O F T H E D N A

Shunji Natori, Tone, Japan assigned to Sanwa Kagaku Kenkyusho Co Ltd

A cloned single-strand DNA encoding amino acid sequence for a eukaryotic transcription fac- tor S-II is disclosed together with, a cloned double-strand DNA consisting of the single- strand DNA and its complementary single- strand DNA, a DNA fragment of the single- or double-strand DNA, a process for the prepara- tion thereof, a plasmid, in which the double- strand DNA or its fragment is inserted, and a diagnosis of viral diseases and cancers.

5196304

S E R I N E P R O T E I N A S E I N H I B I T O R G E N E F R O M T H E I N S E C T

M A N D U C A S E X T A

Michael Kanost, Michael Wells, Sarvamangala V Prasad, Tone, assigned to Arizona Tech- nology Development Corporation

Disclosed is a eDNA clone isolated from a fat body cDNA library from the tobacco hornworm Manduca sexta, and the deduced amino acid sequence of the serine proteinase inhibitor encoded by the eDNA.

5196305

D I A G N O S T I C A N D A M P L I F I C A T I O N M E T H O D S

U S I N G P R I M E R S H A V I N G T H Y M I N E A T 3 ' E N D T O

O V E R C O M E P R I M E R - T A R G E T M I S M A T C H A T T H E 3 ' E N D

John B Findlay, Lynn Bergmeyer, Tone, as- signed to Eastman Kodak Company

Methods for amplifying and detecting a pre- determined target nucleic acid in a biological specimen are accomplished even where there is a mismatch in a single position between a primer and the target nucleic acid. The mismatch is located at or near the 3' end of the primer. Such a mismatch is overcome using a primer having a nucleotide with a thymine base at the position of the mismatch. The use of such primers is most likely to prime the target and form primer exten- sion products. This method is particularly useful for detection of a nucleic acid sequence which is not fully known, or where there is considerable heterogeneity in DNA target from patient sam- ples.

5196307

C L O N E D H U M A N C E N T R O M E R E A U T O A N T I G E N

William Earnshaw, Don Cleveland, Kevin F Sullivan assigned to The Johns Hopkins Univer- sity

The invention relates to DNA molecules coding for human CENP-B polypeptides. The invention provides DNA molecules comprising an epito- pically functional part of the eDNA sequence of human CENP-B polypeptide. The invention also provides cloning vehicles capable of replication and expression comprising eDNA molecules coding for human CENP-B polypeptide. The in- vention further provides for hosts transformed with a vehicle having a eDNA molecule coding for human CENP-B polypeptide. In another em- bodiment, the invention provides for the detection of autoantibodies to human CENP-B using the human CENP-B polypeptide encoded for by the cDNA molecules of the invention. Also, the invention provides monoclonal anti- bodies, and hybridomas which produce them,

PATENT ABSTRACTS

which bind an epitope on human CENP-B which corresponds to an epitope to which auto- antibodies to human CENP-B bind.

5196308

M E T H O D S F O R I D E N T I F Y I N G T H E D Q W 3 . 2 A L L E L E

A S S O C I A T E D W I T H I N C R E A S E D R I S K O F I N S U L I N - D E P E N D E N T

D I A B E T E S M E L L I T U S

Gerald Nepom, Barbara S Nepom, Tone, as- signed to Genetic Systems Corporation

Methods for identifying individuals at increased risk of diabetes are disclosed. The methods dis- closed utilize the discovery of the DQw3.2 variant, which identifies a specific allelic poly- morphism at a sinle gene locus. One preferred method utilizes a labeled probe to detect the DQw3.2 allele. This method involves estimating the size of the hybridizable DNA fragment gene- rated by a specific restriction endonuclease and therefrom determining the presence of the allele. A second preferred method involves the serologic detection of the DQw3.2 allele. Within this method, immunocomplexes formed between two different MAb's and separate portions of a cell collection are detected and the presence or absence of the allele determined.

119

5196318

P R E C I S E L Y R E G U L A T E D E X P R E S S I O N O F D E L E T E R I O U S

G E N E S

Thomas O Baldwin, Jerry Devine, Gerald S Shadel, Takarazuka, assigned to The Texas A&M University System

The invention relates to an expression vector system based on the regulation of bacterial luminescence (the lux gene system). The inven- tion further relates to the construction of a pre- cisely regulatable expression vector system which comprises a complete luxR gene in com- bination with an inactivated luxI gene. If the sys- tem is turned off, no significant transcription occurs of any cloned gene product when used in combination with the regulatory scheme of the invention as is demonstrated by using the bac- teriophage lambda lysis genes. The induction of transcription relies on the addition of exogenous autoinducer which is both inexpensive and easy- to-use and which is required in only minute amounts.

5196316

E N Z Y M E A N D D N A C O D I N G T H E R E F O R

Yasuno Iwasaki, Yoshik Nishikawa, Takarazuka, Japan assigned to Japat Ltd

The invention concerns a peptidylhydroxygly- cine N-C lyase (PHL) catalyzing the reaction See Patent for Tabular Presentation PS wherein R represents a peptide residue, and GIyOH represents an alpha-hydroxygiycine residue linked to the C-terminus of said peptide R by an amide bond, a recombinant DNA molecule coding for a PILL, a method for the preparation of such a recombinant DNA molecule, processes for the preparation of PHL from a natural source or by means of the said recombinant DNA molecule, and the use of PIlL for the pre- paration of an amidated peptide R-NH2.

5196319

P R I M A R Y B I L I A R Y C I R R H O S I S A U T O A N T I G E N

Ross Coppel, Merrill E Gershwin, Victoria, Austrafia assigned to Amrad Corporation Limited

ADNA molecule is provided which comprises a nucleotide sequence substantially corresponding to all or a portion of the base sequence coding for the mitochondrial autoantigen of primary bi- liary cirrhosis. This DNA molecule may be linked to an expression control sequence. A syn- thetic peptide or polypeptide is provided which includes at least an amino acid sequence substan- tially as shown in FIG. 6 or FIG. 8. This syn- thetic peptide or polypeptide may be prepared by expression of a host cell which has been trans- formed with a recombinant DNA molecule as described above, or by chemical synthesis.