PATENT ABSTRACIS

The sequence of the protein coding regions of the bcl-2 gene are provided as well as bacterial clones which produce the proteins. Assays are provided for detecting a class of B-cell neoplasms associated with a chromosome trans- location between chromosomes 14 and 18. The translocation is involved in the majority of cases of human follicular lymphomas. One assay em- ploys an antibody which is immunoreactive with a human protein which is over-expressed due to the chromosome translocation. Another assay involves measurement of the amount of mRNA which hybridizes to the gene proximal to the translocation break-point.

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T R A N S M I S S I B L E G A S T R O E N T E R I T I S V I R U S

G E N E S

David A Brian, Paul A Kapke assigned to University of Tennessee

The present invention provides recombinant DNA molecules comprising a sequence encoding a transmissible gastroenteritis virus polypeptide selected from the group consisting of M, N and P, host cells transformed by said recombinant DNA molecule sequences, the M, N and P poly- peptides. The present invention also provides subunit vaccines for TGEV.

5204015

133

5204096

P R E - S G E N E C O D E D P E P T I D E H E P A T I T I S B I M M U N O G E N S ,

V A C C I N E S , D I A G N O S T I C S , A N D S Y N T H E T I C L I P I D V E S I C L E

C A R R I E R S

Alexander R Neurath, Stephen B Kent assigned to New York Blood Center Inc; California In- stitute of Technolo

A hepatitis B vaccine containing a peptide with an amino acid chain of at least six consecutive amino acids within the pre-S gene coded region of the envelope of hepatitis B virus. The vaccine being free of an amino acid sequence correspon- ding to the naturally occurring envelope proteins of hepatitis B virus and a physiologically ac- ceptable diluent. The peptide being free or linked to a carrier. The carrier being a conventional car- rier or a novel carrier including a lipid vesicle stabilized by cross-linking and having covalently bonded active sites on the outer surface thereon. Such novel carrier being useful not only to link the novel peptide containing an amino acid chain with amino acids within the pre-S gene coded re- gion of the surface antigen or hepatitis B virus, but can also be used to bind synthetic peptide analogues of other viral proteins, as well as bac- terial, allergen and parasitic proteins of man and animals. The peptides of the invention can be utilized in diagnostics for the detection of anti- gens and antibodies.

S U B T I L I S I N M U T A N T S

Robert Caidwell, David A EsteU, Thomas Gray- car, late of Tokyo, assigned to Genencor Inter- national Inc

Novel carbonyl hydrolase mutants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of one or more amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such mutant carbonyl hydrolases have pro- perties which are different from those of the pre- cursor hydrolase and are especially useful in detergent formulations. The substituted amino acid residues correspond to position +123 and/or + 274 in Bacillus amyloliquefaciens sub- tilisin.

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U N I V E R S A L A N D S P E C I F I C P R O B E S F O R B A C I L L U S

T H U R I N G I E N S I S E N D O T O X I N G E N E S

Frank H Gaertner, August J Sick, George E Schwab, late of Tokyo, assigned to Mycogen Corporation

This invention concerns novel gene probes which can be used to identify DNA from Bacillus thuringiensis microbes which encode insecticidally-active protein endotoxins. The in- vention probes greatly facilitate the search for useful microbes hosting genes which encode insecticidally-active toxins.