5.4 penicillinase activity
TRANSCRIPT
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Chemical Warfare
An Illustrated Guide to select Enzyme / Antibiotic Interactions
to Accompany Lecture
By Noel Ways
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Transpeptidase
Transpeptidase
Transpeptidase
Transpeptidase
Bacteria makes transpepti-dase for interpeptide bridge formation of peptidoglycan.The peptidoglycan cell wall enables the bacteria to width-stand osmotic pressures.
Penicillin interferes with transpeptidase. Therefore, interpep-tide bridges of pepti-doglycan cannot be made and the cell wall is compromised
Bacteria can no longer withstand osmotic pressures and lysis, as it divides and grows.
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Transpeptidase
Transpeptidase
Transpeptidase
Transpeptidase
Bacteria acquire plasmid born β-lactamase ( ) (penicillinase) capability. β-lactamase breaks β-lactam ring rendering penicillin ine�ective against transpep-tidase. Peptidoglycan syn-thesis proceeds normally as cell divides and grows.
β-lactamase inhibitors ( ) added to penicillin. By competitive inhibition, β-lactamase inhibitors bind to β-lactamase preventing it from e�ecting the peni-cillin. Penicillin now ren-ders transpeptidase inef-fective.
β-lactamase
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Plasmid with gene that codesfor β-lactamase
Penicillin(with it’s β-lactam ring)
Plasmid with gene that codesfor β-lactamase
β-lactamase inhibitor bindsto β-lactamaseβ-lactamase
Penicillin withβ-lactamase inhibitors ( )
Penicillin uneffectedand continues to function to disrupt Transpeptidase
Bacterial cell wall disrupted.Bacteria dies
β-lactamase inhibitor
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