2

CONTENTS

1. Vision, Mission and Program Educational Objectives (PEO) 3 2. Program Outcomes (PO) with Graduate Attributes 4 3. Mapping of POs with PEOs 5

SCHEME

4. Scheme of Third Semester B.E 6 5. Scheme of Fourth Semester B.E 7

SYLLABUS

6. Syllabus of Third Semester BE: a) Engineering Mathematics-III 9 b) Unit Operations for Bioengineering 12 c) Cell Biology & Genetics 16 d) Biochemistry & Structural Biology 20 e) Biochemical Calculations 24

7. Syllabus of Fourth Semester BE: a) Biostatistics & Biomodeling 28 b) Microbiology 31 c) Molecular Biology 35 d) Biochemical Thermodynamics 39 e) Biophysics & Bioanalytical Techniques 43

8. Syllabus for Common Subjects a) Economics for Engineers 46 b) Life Skills for Engineers 48

Appendix A Outcome Based Education 50 Appendix B Graduate Parameters as defined by National Board of Accreditation 51 Appendix C Bloom’s Taxonomy 52

3

VISION

To produce proficient Biotechnology Engineers with inventive and disseminative abilities for the societal and environmental benefit by enriching their technical and managerial skills.

MISSION

• To empower students and faculty with strong fundamental and applied knowledge inBiotechnology engineering by fostering their innovative and inventive skills.

• To enrich students with the essential skills by providing strong industry relevantcurriculum developed through a functional industry academia interface.

• To imbibe students with professional, moral and ethical values to serve Biotechnologyprofession by encouraging their active participation in co and extracurricular activities.

Program Education objectives (PEOs)

PEO1 Solid foundation in the principles and practices of Biotechnology along with holistic knowledge in Science, Mathematics and basic Engineering.

PEO2 Develop independent thinking and problem solving capacities thereby producing good researchers with high applicability in Industry.

PEO3 Imbibe concepts of ethics, integrity, leadership and social responsibility in the graduates.

PEO4 Transform the graduates into competent performers with effective communication skills and capacities.

PEO5 Prepare graduates for their careers in Biotechnology industry or higher studies. PEO to Mission Statement Mapping

Mission Statement PEO 1 PEO 2 PEO 3 PEO 4 PEO 5 Provide a holistic environment to educate the students with fundamental and advanced concepts of Biotechnology

3 2 1 2 2

Inculcate critical and lateral thinking capacities by improving their knowledge in Biotechnology allied fields through basic and applied outcome based research

2 3 2 1 2

Empower them to acquire strong ethical and intellectual capabilities along with societal and environmental awareness

2 1 3 3 2

Enable the students to develop appropriate skill sets useful both for working with multi-disciplinary – heterogeneous teams and also continuing education

2 3 3 3 3

4

Correlation: 3- High, 2-Medium, 1-Low

Program Outcomes (PO) with Graduate Attributes

Graduate Attributes Program Outcomes (POs)

1 Engineering knowledge

PO1: Developing profound knowledge of the fundamentals and advancements in Biotechnology domain and related engineering courses.

2 Problem analysis PO2: Analyze conditions of usefulness involved in theory and lab courses problem solving.

3 Design and Development of Solutions

PO3: Coming out with an optimized solution to the real time problems and processes.

4 Investigation of Problem

PO4: Investigating the efficacy of the chosen solution and trying the same with the alternative and cost effective tools with enhanced performance.

5 Modern tool usage PO5: Enhancing the Proficiency and knowhow of the advanced techniques and testing tools in the field.

6 Engineer and society PO6: Educating and making a socially responsible Engineer with appreciable applicability towards mankind and society at large.

7 Environment and sustainability

PO7: Inculcating the consciousness about the need of protecting environment and ensuring safety to the mankind.

8 Ethics PO8: Educating the importance of being bound to ethics for the benefit of society.

9 Individual & team work PO9: Transforming into a performer capable both as an individual and also as a team player.

10 Communication PO10: Developing good skills of communication for becoming a competent performer.

11 Lifelong learning PO11: Keeping updated with the recent advancements in the domain.

12 Project management and finance

PO12: Inculcating managerial skills bearing financial aspects on a project (academic / entrepreneurial).

5

Mapping of POs TO PEOs

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12 PEO1 3 2 2 2 2 1 2 2 1 1 3 2 PEO2 1 3 3 3 3 1 2 1 3 2 1 2 PEO3 1 1 1 2 1 3 3 3 3 2 2 3 PEO4 2 2 2 2 2 2 1 1 2 3 2 3 PEO5 2 3 2 3 2 2 1 2 2 2 2 3

Correlation: 3- High, 2-Medium, 1-Low

6

New Horizon College of Engineering Department of Biotechnology Engineering

Scheme of Third Semester B.E Program

Sl. No

Course Code

Course Credit Distribution Overall Credits

Contact Hours

weekly-Theory

Contact Hours

weekly-(Lab)

Marks

CIE SEE Total

L P T S

1 MAT31 Engineering Mathematics-III

4 0 1 0 5 6 0 50 50 100

2 HSS321 Economics for Engineers

2 0 0 1 3 2 0 50 50 100 HSS322 Life Skills for

Engineers

3 BTE33 Unit Operations for Bioengineering

2 2 1 0 5 4 4 75 75 150

4 BTE34 Cell Biology & Genetics

3 2 0 0 5 3 4 75 75 150

5 BTE35 Biochemistry & Structural Biology

3 2 0 0 5 3 4 75 75 150

6 BTE36 Biochemical Calculations

3 0 0 1 4 2 0 50 50 100

Total 27 20 12 375 375 750

BS, 5

HSS, 3

ES, 5

PS-Core, 1

4

Credit Distribution Across Various Subject Areas

Lecture

59%

Practical

35%

T6%

Contact Hours per week (Percentage)

26%

17%27%

14%

10%6%

Average Assessment of various Bloom's levels (%)

Remember

Understand

Apply

Analyze

Evaluate

Create

7

New Horizon College of Engineering Department of Biotechnology Engineering

Fourth Semester B.E Program-Scheme

Sl.No

Course Code

Course Credit Distribution Overall Credits

Contact Hours

weekly Theory

Contact Hours

weekly (Lab)

Marks

CIE SEE Tot

L P T S

1 BTE41 Biostatistics & Biomodeling

4 0 1 0 5 6 0 50 50 100

2 HSS421 Economics for Engineers

2 0 0 1 3 2 0 50 50 100 HSS422 Life Skills for

Engineers

3 BTE43 Microbiology 3 2 0 0 5 3 4 75 75 150

4 BTE44 Molecular Biology

3 2 0 0 5 3 4 75 75 150

5 BTE45 Biochemical Thermodynamics

3 2 0 0 5 3 4 75 75 150

6 BTE46 Biophysics & Bioanalytical Techniques

3 0 1 1 5 5 0 50 50 100

Total 28 22 12 375 375 750

BS18%

HSS11%

PS-Core71%

Credit Distribution Across Various Subject Areas

Lecture58%

Practical

32%

T10%

Contact Hours per week (Percentage)

24%

16%

25%

12%

10%

13%

Average Assessment of various Bloom's levels (%)

Remember

Understand

Apply

Analyze

Evaluate

Create

8

THIRD SEMESTER

(SYLLABUS)

9

ENGINEERING MATHEMATICS – III (Common to All Branches)

Course Code : MAT31 Credits : 05

L: P: T: S : 4:0:1:0 CIE Marks : 50

Exam Hours : 3 SEE Marks : 50

Course Outcomes: At the end of the Course, the student will be able:

CO1 Solve the Fourier series expansion of functions analytically and numerically.

CO2 Solve the Continuous model a problem using Fourier transforms.

CO3 Solve the discrete model problems using Z-transforms and Fast Fourier transform.

CO4 Fit a suitable curve by the method of least squares and determine the lines of regression for a

set of statistical data.

CO5 Use appropriate numerical methods to solve algebraic and transcendental equations and also

to calculate a definite integral.

CO6 Use appropriate numerical methods to solve Boundary Value Problems in Partial differential

equations.

Mapping of Course Outcomes to Program Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 POI0 POII PO12

CO1 3 3 1 1 1 - - - 2 1 - 2

CO2 3 3 1 1 1 - - - 1 1 - 2

CO3 3 3 2 3 3 - - - 2 1 - 2

CO4 2 3 2 2 2 - - - 1 3 - 1

CO5 2 2 3 3 2 - - - 1 2 - 1

CO6 3 3 3 2 3 - - - 2 1 - 1

10

Course Syllabus Module No.

Contents of the Module Hours CO’S

1 Fourier series: Periodic function, Dirichlet’s conditions, Fourier series of periodic functions of period 2π and arbitrary period 2l , half range series. Fourier series and half Range Fourier series of periodic square wave, half wave rectifier, full wave rectifier, Saw-tooth wave with graphical representation, practical harmonic analysis.

9 CO1

2 Fourier Transforms: Infinite Fourier transforms, Fourier Sine and Cosine transforms, Inverse Fourier transform. Z - Transform: Definition, Z-transforms of some standard functions, properties, damping rule, shifting rule(without proof), initial and final value theorems, inverse Z- transforms. Applications: Solving difference equations using Z-transform.

9 CO1, CO2

3 Statistical Methods: Fitting of the curves of the form ,y a b x= + 2 ,y a b x c x= + + ,bxy a e= , andby a x= xy ab= by the method of

least square, Correlation and Regression, Regression coefficients, line of regression – problems. Discrete Fourier Transform and Fast Fourier Transform: Definition of N-Point DFT, problems for 4-Points and inverse DFT for four points only. FFT algorithm to compute the Fourier transforms 4-Point only.

9 CO3, CO4

4 Numerical Methods-1: Numerical solution of algebraic and transcendental equations; Rugula- falsi method and Newton Raphson’s method. Solution of a system of equations using Gauss-seidel and Relaxation method. Interpolation and extrapolation – Newton’s forward and backward formulae for equal intervals, Newton divided difference and Lagrange’s formulae for unequal intervals.

9 CO5

5 Numerical Methods-2: Numerical integration - Simpson’s 1/3rd rule, Simpson’s 3/8th rule,Weddle‘s rule (without proof)- Problems. Numerical solution of Boundary value problems-Solution of one dimensional wave equation and heat equation, Numerical solution of two dimensional Laplace’s equation and Poisson’s equation.

9

CO5, CO6

Text Books:

1. Erwin Kreyszig, Advanced Engineering Mathematics, Wiley-India Publishers, 10th Edition, 2014,ISBN: 978-81-265-5423-2. 2. B. S. Grewal, Higher Engineering Mathematics, Khanna Publishers, 43rd Edition, 2014, ISBN: 978-81-7409-195-5.

11

Reference Books:

1. Glyn James, Modern Engineering Mathematics, Prentice Hall, 4th Edition, 2015, ISBN: 978-0-273-73409-3 2. B. V. Ramana, Higher Engineering Mathematics, McGraw Hill Education (India) Private

Limited, 4th Edition, 2016, ISBN: 978-0-07-063419-0. 3. H. K. Dass, Advanced Engineering Mathematics, S. Chand & Company Ltd., 28th Edition, 2012, ISBN: 81-219-0345-9. 4. N.P.Bali and Manish Goyal, A Text Book of Engineering Mathematics, Laxmi Publications (P) Ltd., 9th Edition, 2014, ISBN: 978-81-318-0832-0. Assessment Pattern:

1. CIE- Continuous Internal Evaluation (50 Marks). Bloom’s Category

Tests (25 Marks)

Assignments (10 Marks )

Quizzes (5 Marks )

Curricular/Co-Curricular Activities (10 Marks )

Remember 5 5 -- -- Understand 5 5 -- -- Apply 5 -- 5 10 Analyze 5 -- -- -- Evaluate 5 -- -- -- Create -- -- -- --

2. SEE- Semester End Examination (50 Marks).

Bloom’s Category Questions (50 Marks) Remember 10 Understand 10 Apply 20 Analyze 5 Evaluate 5 Create --

12

UNIT OPERATIONS FOR BIOENGINEERS Course Code : BTE33 Credits : 05 L: P: T: S : 2:2:1:0 CIE Marks : 50+25 Exam Hours : 3+3 SEE Marks : 50+25 Course Outcomes: At the end of the Course, the Student will be able to: CO1 Develop Basic knowledge of the Unit Operations and apply the principles of Unit

operations in bioindustries CO2 Enhance Knowledge and practical skills in understanding fluid properties and laws

governing the flow and apply it to biological sciences CO3 Learn principle and working of Pumps and metering devices and develop skills to

operate and evaluate metering devices CO4 Apply dimensional analysis techniques to obtain relations between the variables and

also understand the dynamics of scale up studies from lab to industrial CO5 Develop problem solving capability and become a competent Bioengineer CO6 Design the various Unit operation equipments for Biological Systems in food industry,

pharma industry and other related industries Mapping of Course Outcomes to Program Outcomes: PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12 CO1 3 3 1 - 1 - - - - - 2 - CO2 3 2 1 1 - - 2 - - - 2 - CO3 3 2 2 2 - - - - - - 1 - CO4 2 2 2 2 1 - 2 - 1 1 1 2 CO5 2 3 2 2 1 1 2 2 2 3 2 2 CO6 3 3 3 2 2 1 2 1 2 - 2 2

Module No.

Module Contents Hours COs

1

Dimensional analysis: Units, Dimensions, Basic and Derived units, Dimensional homogeneity, Dimensionless numbers, Rayleigh’s method, Buckingham’s pi theorem. Particle size analysis: Sieves, differential and cumulative screen analysis. Screens- Ideal and actual screens. Industrial screening equipment. Size reduction- Laws of Size reduction, Work Index, Equipment for size reduction- Ball mill

9

CO1,CO4

List of Experiments 1. Determination of effectiveness of a screen 2. Size reduction by using ball mil

8

13

2

Introduction To Fluid Mechanics: Fluid definition and classification (Types of fluids – Newtonian and Non Newtonian); Pressure measurement – Manometers Flow through conduits: Types of flow - laminar and Turbulent; Reynolds number; Basic equations of fluid flow - Continuity equation and Bernoulli equation; Flow through circular and non circular conduits – Hagen Poiseuille equation; Conceptual Numericals

9

CO2,CO3,CO4 List of Experiments

1. Reynold’s Experiment to study laminar, Turbulent and Transition flow

2. Friction in circular and non circular pipes

8

3

Settling: Types of settling: Free and hindered settling. Terminal velocity, equation for one dimensional motion of particle through a fluid in gravitational field. Motion of particles in Stoke’s, Newton’s and intermediate, centrifugal settling process. Conceptual problems. Sedimentation: Batch settling test, Kynch’s Theory, Thickener design (derivation not required, only final equation) Determination of thickener area. Filtration: Introduction, Classification of filtration, types, Characteristics of filter media & filter aids, application, principles of cake filtration, Industrial filters- rotary drum filter, leaf filter and plate and frame filter press.

9

CO2, CO6

List of Experiments 1. Verification of Stoke’s law in Free Settling. 2. Determination of minimum thickener area using Batch

sedimentation tests 3. Constant pressure filtration using leaf filter and with cloths o

different pore sizes.

8

4

Transportation and metering of fluids: Construction and working o Centrifugal pump, reciprocating pump, pumping energy requiremen calculations. Characteristics of centrifugal pumps, cavitation, NPSH. Fluid flow measurement- Venturimeter, Orifice meter, Pitot tube, Rotameter, Magnetic meters

9

CO3 List of Experiments

1. Flow rate measurement using orificemeter 2. Flow rate measurement using Venturimeter 3. Study of pump Characteristics. 4. Measurement of pressure drop vs flow rate

8

5 Basics of Heat and Mass Transfer: Heat Transfer: Modes of heat transfer; Conduction – steady state heat conduction through uni-layer and multilayer walls, cylinders;

9 CO5,CO6

14

Insulation, forced and Natural convection; Condensation – film wise and drop wise (no derivation); Heat transfer equipments – double pipe heat exchanger, shell and tube heat exchanger. Conceptual numerical. Mass Transfer Diffusion – Fick’s law of diffusion. Measurement of diffusivity Distillation – Methods of distillation, distillation of binary mixtures using McCabe Thiele method; principles of leaching, extraction, Conceptual numerical. List of Experiments 1. Diffusion of organic solvent in air. 2. Simple Distillation 3. Liquid – liquid Extraction

8

Text Books:

1. Unit Operations in Chemical Engineering, W. L. McCabe, J. C. Smith and P. Harriott, McGraw-Hill Publishers, 7th Edition, , 2005, ISBN: 0072848235, 9780072848236.

2. Chemical Engineering VoI 1. Fluid flow, Heat Transfer and Mass Transfer J.M.Coulson and J.F.Richardso, , Indian Reprint, 6th Edition, 2000, ISBN: 0750644443, 9780750644440.

Reference Books: 1. Principles Of Unit Operations, 2Nd Ed, Alan S Foust, L.A. Wenzel, C.W. Clump, L. Maus, and

L.B. Anderson, John Wiley & Sons. Reprint 2008, ISBN: 8126518294, 9788126518296. 2. Transport Processes and Separation Process Principles: Includes Unit Operations, C. J.

Geankoplis, Prentice Hall India, illustrated, reprint, 4th edition, 2003, ISBN: 013101367X, 9780131013674.

3. Unit Operations in Food Engineering, Albert Ibarz, Gustavo V. Barbosa-Canovas, CRC Press, 2002, ISBN: 1420012622, 9781420012620.

4. Unit Operations Handbook: Volume 2 (In Two Volumes), John J. McKetta Jr, CRC Press, 1992, ISBN: 082478670X, 9780824786700.

15

Assessment Pattern CIE- Continuous Internal Evaluation: Theory (50 Marks)

Bloom’s Taxonomy

Tests (25 Marks)

Assignments (10 Marks )

Quizzes (5 Marks )

SSA (10 Marks)

Remember 5 - 2 - Understand 5 3 2 2

Apply 5 3 1 2 Analyze 5 2 - 2 Evaluate 5 1 - 2 Create - 1 - 2

CIE- Continuous Internal Evaluation: Lab (25 Marks)

Bloom’s Category Lab Marks (out of 25)

Remember 5 Understand 5

Apply 10 Analyze 3 Evaluate 1 Create 1

SEE- Semester End Examination: Theory (50 Marks) Bloom’s Category Tests Marks (out of 50) Remember 20 Understand 10 Apply 10 Analyze 5 Evaluate 5 Create - SEE- Semester End Examination: Lab (25 Marks)

Bloom’s Category Lab Marks (out of 25)

Remember 5 Understand 5

Apply 10 Analyze 3 Evaluate 1 Create 1

16

CELL BIOLOGY & GENETICS

Course Code : BTE34 Credits: 05 L:P:T:S : 3:2:0:0 CIE Marks: 50+25 Exam Hours : 03 SEE Marks: 50+25

Course Outcomes: At the end of the Course, the Student will be able to: CO1 Develop a strong background in interface of cell biology, Cytogenetics and Genetic

aspects of cell and be able to use these knowledge in the research and applied fields of biotechnology

CO2 Understand the cell at structural level and analyze at functional level

CO3 Encompass broad knowledge on the cell cycle and the dynamics of the living cell CO4 Apply the Laws of Mendelian inheritance to understand the mechanism of Sex

determination and Sex linked disease. CO5 Demonstrate a clear understanding of the signal transduction mechanism, secondary

signal transduction and the process of cell growth. CO6 Understand the process and importance of evolution by knowing the basic principles of

population and evolutionary genetics. Mapping of Course Outcomes to Program Outcomes: PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12 CO1 3 3 3 2 2 1 1 2 2 1 3 3 CO2 3 3 1 - - 1 - - 2 1 3 - CO3 3 3 1 1 1 1 - - 2 1 1 - CO4 3 3 3 1 2 2 - - 3 1 3 - CO5 3 2 3 - 1 1 - - 2 2 2 - CO6 3 3 3 2 1 3 - 1 3 2 2 2

Module No

Module Contents Hours Cos

1

Cell organization & Function of the organelles: Basic concepts and cell theory, Structure and function of prokaryotic & eukaryotic cells. Cell membrane (Plasma membrane): Structural composition, organization and functions. Organelles of eukaryotic cell - structure and functions: Cytoplasm, Nucleus, Mitochondria, Chloroplast, Endoplasmic reticulum, Golgi bodies, Ribosomes, Lysosomes, Peroxisomes and Vacuoles. Cell markers and determinants.

8 CO1

List of Experiments 8

17

1. Basic principles of microscopy and microscopic measurements.

2. Study of cellular fractionation by homogenization of seedlings and differential centrifugation.

2

The cell architecture and locomotion: Cytoskeleton and its components; structural and functional organization of Microtubules, Microfilaments and Intermediate filaments. Cytoplasmic microtrabecular system (lattice). Intracellular communication & Cell-Cell junctions, Cell adhesion. Membrane Trafficking, Cell locomotion: Ameoboid, Flagella, Cillar. Protoplast and spheroplast

9

CO1, CO2 List of Experiments 1. Plasma membrane: Osmosis in plant Leaf Cells (or)

Human Blood Cells 2. Isolation of plant protoplasts by mechanical method

and Chemical fusion of plant protoplasts (PEG, Calcium)

3. Motility of bacteria by hanging drop method.

8

3

Cell cycle & Signal transduction: Eukaryotic cell cycle; phases and regulation. Cell division: Mitosis and Meiosis. Signal transduction: Cell signaling models (autocrine, paracrine, and endocrine; signal molecules and their types. Cell surface Receptors: Ion channel linked, G protein linked and enzyme linked Receptors; Intracellular signal transduction: Secondary signal molecules, Cytoplasmic receptors, Nuclear receptors, Cell Growth: Hemopioesis and Regulation.

9

CO3, CO5 List of Experiments

1. Study of various divisional stages in Mitosis using cytological preparation of onion root tips.

2. Study of various divisional stages in Meiosis using cytological preparation of onion flower buds.

3. Study of Polygene and Lamp brush chromosomes using permanent slides.

8

4

Cytogenetics & Heredity: Genetic material: classical experiments- Hershey & Chase, Avery, McLeod, Structure and organization of eukaryotic chromosome, Euchromatin and heterochromatin. Mendelian genetics: Mendel laws of inheritance, Monohybrid and Dihybrid inheritance. Neo-Mendelian genetics: Incomplete Dominance, Co-dominance, Supplementary genes, Complementary genes, Inhibitory, coloured genes and simple problems. Heredity: Linkage and crossing over, Sex determination in plants & animals; Sex linked disease- hemophilia; Autosomal linked disease-color

10 CO4

18

blindness. List of Experiments

1. Solving genetic problems related to monohybrid and dihybrid ratio (minimum of six problems in each topic)

2. Solving genetic problems related to gene interaction (minimum of six problems in each topic)

8

5

Population and Evolutionary Genetics: Population genetics: Introduction, Size and genetic structure of Population, Gene frequency changes and equilibrium estimation, Hardy-Weinberg principle for closed and open populations. Evolutionary genetics: Types of mutations; Spontaneous and Induced; significance of mutation. Chromosomal aberrations. Point mutations & frame shift mutations, Concepts and case studies of Evolutionary genetics. Prospects for the control of human evolution.

9

CO6

List of Experiments 1. Construction of linkage maps, two point test cross. 2. Reading of dendrogram and analysis with case

studies.

8

Text Books:

1. Principles of Genetics, Gardner, M. J. Simmons, D. P. Snustad, , John Wiley & Sons, 8th edition, 2006, ISBN: 8126510439, 9788126510436.

2. Cell Biology, S C Rastogi, New Age International, 3rd edition, 2005, ISBN: 8122416888, 9788122416886.

Reference Books:

1. Volume 1 - Cell Biology and Genetics, Cecie Starr, Ralph Taggart,Christine Evers, Lisa Starr, Cengage Learning, 14th Edition, 2015, ISBN: 130572481X, 9781305724815.

2. Cell and Molecular Biology: A Lab Manual, Chaitanya K. V., PHI Learning Pvt Ltd, 1st edition, 2013, ISBN: 8120348001, 9788120348004.

3. Genetics, Susan Elord & William Stansfield, , Tata-McGraw-Hill Publishing Co. Ltd., IV Edition, 2010, ISBN: 0070139199, 9780070139190.

4. Cell Biology and Genetics, Joanne Evans, Ania L. Manson, Mosby, 3rd edition, 2008, ISBN: 0723434212, 9780723434214.

19

Assessment Pattern CIE- Continuous Internal Evaluation: Theory (50 Marks)

Bloom’s Taxonomy

Tests (25 Marks)

Assignments (10 Marks )

Quizzes (5 Marks )

Curricular / Cocurricular (10 Marks)

Remember 10 - - - Understand 10 - - 5

Apply 5 5 2 5 Analyze - - 2 - Evaluate - - 1 - Create - 5 - -

CIE- Continuous Internal Evaluation: Lab (25 Marks)

Bloom’s Category Lab Marks (out of 25)

Remember - Understand 3

Apply 10 Analyze 7 Evaluate 5 Create -

SEE- Semester End Examination: Theory (50 Marks) Bloom’s Category Tests Marks (out of 50) Remember 10 Understand 7 Apply 20 Analyze 8 Evaluate 5 Create -

20

SEE- Semester End Examination: Lab (25 Marks) Bloom’s Category Lab Marks (out of 25) Remember - Understand 3 Apply 10 Analyze 7 Evaluate 5 Create -

BIOCHEMISTRY & STRUCTURAL BIOLOGY

Course Code: BTE35 Credits : 05 L: P: T: S : 3:2:0:0 CIE Marks : 50+25 Exam Hours : 03 SEE Marks : 50+25 Course Outcomes: At the end of the Course, the Student will be able to

CO1 Develop an understanding about the existence of biomolecules in a living cell and the analytical methods to analyze them.

CO2 Understand the basic chemical nature of biomolecules - the concepts of chemical structures and conformations in biomolecules.

CO3 Learn the role of different biomolecules in various cell processes. CO4 Understand the complex metabolic processes involved in the living cell. CO5 Know about the dynamic relationship between structure and function CO6 Develop a profound knowledge on the structural complexities involved in biomolecules

Mapping of Course Outcomes to Program Outcomes: PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12 CO1 3 3 3 2 2 1 1 2 1 1 3 2 CO2 3 3 1 - - 1 1 1 1 1 3 1 CO3 3 3 1 1 1 1 - - 2 1 1 1 CO4 3 3 3 1 2 2 - - 1 1 3 - CO5 3 2 3 - 1 1 1 - 2 2 2 - CO6 3 3 3 2 1 3 - 1 1 2 2 1 Module No Module Contents Hours COs

1 Chemistry of Carbohydrates: Classification, occurrence, structure and function, stereo and optical isomerism of sugars, Glucose, Fructose, Galactose, Maltose, Lactose, sucrose, starch,

9 CO1 CO2

21

glycogen and cellulose. amino sugars, Sialic acid, mucopolysaccharides, Glycoproteins, proteoglycans Blood group sugar compounds, sugar nucleotides, Lectins , pectin & xylans.

List of Experiments: 1. Preparation of solution with different concentrations

(w/v, v/v), Normality and Molarity. 2. Measurement of pH and preparation of different buffer

solutions. 3. Qualitative tests for Carbohydrates 4. Estimation of reducing Sugar by O-Toludine / DNS

method.

8

2

Chemistry of Lipids: Classification, occurrence, structure and function, fatty acids (Essential Fatty Acids) prostaglandins, tri-acyl glycerol. phospholipids (esp. lecithin cephalin, phospotidyl inositol and phospotidyl serine) spingo myelin, plasmologens. Glycolipids & cholesterol.

9 CO1 CO2

List of Experiments: 1. Determination of Saponification value of Lipids. 2. Determination of Iodine value of Lipid. 3. Determination of Peroxide value.

8

3

Metabolism of Biomolecules: Glycolysis, TCA cycle, Calvin Cycle, Pentose Phosphate Pathway. Electron transport chain, Gluconeogenesis, Biosynthesis & Biodegradation of fatty acids, amino acids & nucleic acids, Disorders of metabolism.

9 CO1 CO3 CO4

List of Experiments: 1. Estimation of inorganic Phosphate by Fiske-Subbarao

method. 2. Estimation of phenol by FC method.

8

4

Structure of Proteins: Classification and structure of amino acids based on IUPAC/IUB, Composition and Relationship between primary, Secondary, tertiary and quaternary structures of proteins, Conformational analysis and forces that determine protein structures, geometries, potential energy calculations, phi, psi, omega angles, Ramachandran plot, general features and thermodynamic aspects of protein folding, folding kinetics.

9 CO1 CO2 CO5 CO6

List of Experiments:

1. Qualitative tests for Amino acids and Proteins. 2. Estimation of Protein by Lowry’s and Bradford’s methods.

8

5

Structure of Nucleic acids: General characteristics of nucleic acid structures (A, T, G, C, U), Nucleosides (glycosidic bond ) and Nucleotides (phosphodiester bond), rotational isomers, geometries, Ribose puckering force, base pairing , base stacking, Stabilizing ordered forms of DNA (A, B and Z), tertiary structure of DNA (Supercoiled DNA) & tRNA.

9

CO1 CO2 CO5 CO6

22

List of Experiments:

1. Estimation of DNA & RNA by Spectrophotometric methods.

8

Text Books:

1. Lehninger Principles of Biochemistry, David L. Nelson, Michel M. Cox, W.H. Freeman, 6thEdition, 2013, ISBN: 1464109621, 9781464109621.

2. Fundamentals of Biochemistry: Life at the Molecular Level, Donald J. Voet, Judith G. Voet, Charlotte W. Pratt, John Wiley & Sons, 4th edition, 2013, ISBN: 0470547847, 9780470547847.

Reference Books

1. Harper’s Illustrated Biochemistry, Robert K Murray, Daryl K Granner, Peter A Mayes, McGraw Hill Book Company, 29th Edition., 2012, ISBN: 0071809694, 9780071809696.

2. Biochemistry, Jereny M. Berg, John L. Tymoczko, Lubert Stryer, , W H Freeman Publisher, 6th Edition 2010, ISBN: 1429235020, 9781429235020.

3. Textbook of Structural Biology, Anders Liljas, World Scientific, Reprint edition, 2009, ISBN: 9812772073, 9789812772077.

4. Experimental Biochemistry: A Student Companion, Beedu Sashidhar Rao, Vijay Deshpande, Anshan, Illustrated edition, 2006, ISBN: 1904798519, 9781904798514.

Assessment Pattern CIE- Continuous Internal Evaluation: Theory (50 Marks)

Bloom’s Taxonomy

Tests (25 Marks)

Assignments (10 Marks )

Quizzes (5 Marks )

Curricular / Cocurricular (10 Marks)

Remember 10 - - - Understand 10 4 2 5

Apply 5 4 2 5 Analyze - 2 1 - Evaluate - - - - Create - - - -

CIE- Continuous Internal Evaluation: Lab (25 Marks)

Bloom’s Category Lab Marks (out of 25)

Remember 5

23

Understand 5 Apply 10

Analyze 5 Evaluate - Create -

SEE- Semester End Examination: Theory (50 Marks) Bloom’s Category Tests Marks(Out of 50) Remember 15 Understand 15 Apply 20 Analyze - Evaluate - Create -

SEE- Semester End Examination Lab (25 Marks) Bloom’s Category Lab Marks(Out of 25) Remember 5 Understand 5 Apply 10 Analyze 5 Evaluate - Create -

24

BIOCHEMICAL CALCULATIONS Course Code : BTE36 Credits : 04 L:P:T:S : 3:0:0:1 CIE Marks : 50 Exam Hours : 03 SEE Marks : 50 Course Outcomes: At the end of the Course, the Student will be able to: CO1 Learn the basics concepts of calculations, units and measurements. CO2 Know the mathematical methods used in the Biochemical laboratory procedures. CO3 Know the calculation steps for preparing stock solutions, buffers & reagents. CO4 Predict stoichiometric requirements of reactants and products in biochemical reactions. CO5 Conduct material and elemental balances around steady- state multi-unit processes

without chemical reactions. CO6 Understand and serve the requirements of a bioprocess industry with modern tools Mapping of Course Outcomes to Program Outcomes: PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12 CO1 3 3 - - - - - - - - 1 - CO2 2 3 1 - - - - - - - 1 - CO3 2 3 1 - - - - - - - 1 - CO4 3 2 1 2 - 1 - - - - - - CO5 3 3 2 1 - 2 - - 1 - - - CO6 3 1 2 2 3 3 1 1 1 1 2 2 Module No

Module Contents Hours COs

1

Concept of atom and mole. Expressing compositions - Solids, liquids and gases. Solutions and mixtures. Weight and Volume, %, w/v, w/w, Specific Gravity, Normality, Molarity, Molality, , Osmolarity, ppm and ppb. Conceptual numerical.

9

CO1 CO2

2

Acids and Bases, Ionization of strong and weak acids / bases. Determination of pH, Measurement of pH. Equilibrium Constants for Weak acids and Bases, Relationship of Ka, Kb, [H3O+], and pH; Dissociation constant, Henderson-Hasselbalch Equation – derivation and Limitations. Titration curves of Strong/Weak acids and bases. Definition and Types of buffers. Buffer Preparation. Conceptual numerical.

9

CO2 CO3

25

3

Stoichiometry of Bioprocess. Introduction, definitions and determination of specific growth rate and yield. Stiochiometry of microbial growth and product formation. Conceptual numerical.

9

CO4 CO6

4

Material balance without chemical reactions. Generalized material balance equations and calculation for distillation, extraction, crystallization, mixing, drying and evaporation operations.

9

CO5 CO6

5

Energy balance. General energy balance equation for steady state. Heat capacity, estimation of heat capacity for solids, liquids, gases and their mixtures. Enthalpy, Standard Heat of formation, standard heat of reaction, Standard heat of combustion and calorific value, Calculation of (HR) at elevated temperature.

9

CO5 CO6

Text Books:

1. Calculations for molecular biology and biotechnology: a guide to mathematics in the laboratory, Stephenson, Frank H., Academic Press, 2nd edition. 2016, ISBN: 0128025980, 9780128025987.

2. Biochemical Calculations, Irwin Segel, John Wiley & Sons, 2nd Edition, 2008 (digital), ISBN: 0471774219, 9780471774211.

Reference Books:

1. e-Study Guide for: Biochemical Calculations: How to Solve Mathematical Problems in General Biochemistry, Irwin H. Segel, Cram101 Textbook Reviews, 22nd Edition, 2014, ISBN: 1467285188, 9781467285186.

2. Protein Purification: Principles and Practice, Scopes, Robert K., Springer Science & Business Media, 2nd Edition, 2013, ISBN: 1475719574, 9781475719574.

3. Fundamentals of Biochemical Calculations, Krish Moorthy, CRC Press, 2nd Edition, 2007, ISBN: 1420053582, 9781420053586.

4. Proteins: Biochemistry and Biotechnology, Walsh, Gary, John Wiley & Sons, Illustrated reprint Edition, 2002, ISBN: 0471899070, 9780471899075.

26

Assessment Pattern

CIE- Continuous Internal Evaluation (50 Marks)

Bloom’s Taxonomy

Tests (25 Marks)

Assignments (10 Marks )

Quizzes (5 Marks )

Curricular / Cocurricular (10 Marks)

Remember 10 - 2 - Understand 10 4 2 5

Apply 5 4 1 5 Analyze - 2 - - Evaluate - - - - Create - - - -

SEE- Semester End Examination (50 Marks) Bloom’s Category Tests Marks(Out of 50) Remember 30 Understand 10 Apply 10 Analyze - Evaluate - Create -

27

FOURTH SEMESTER

(SYLLABUS)

28

BIOSTATISTICS AND BIOMODELING

Course Code : BTE41 Credits: 05 L: P: T: S : 4:0:1:0 CIE Marks: 50 Exam Hours : 03 SEE Marks: 50 Course Outcomes: At the end of the Course, the Student will be able to do the following: CO1 Know and apply the different statistical tools for data analysis CO2 Analyze and solve real time problems involved in bioprocesses and population studies

using probability methods CO3 Analyze, interpret, and evaluate scientific hypotheses and theories using statistical

methods CO4 Understand the concept of modeling using statistical tools CO5 Evaluate and optimize bioprocesses using statistical models. Mapping of Course Outcomes to Program Outcomes: PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12 CO1 3 3 2 - 3 - - - - - 2 - CO2 3 3 3 2 - 1 1 - 1 - - - CO3 3 3 2 1 - - - - 1 1 - - CO4 3 - - - 2 - - - - - 2 - CO5 3 3 1 2 - - - - 1 - - 1 Module No

Module Contents Hours COs

1

Introduction to statistical biology: basic concepts, classification, data

types, frequency distribution, variable, attribute, population, sample, us

of random number tables for drawing a random sample, need for

statistical techniques for biological applications, replicable data,

tabulation of data, construction of graph and graphical representations o

data. Different models of data presentations.

9 CO1

2

Data analysis: Organization of data, central tendency, Calculation of

mean, median and mode, SD, SE, Mean deviation, dispersion,

Skewness and Kurtosis and their various measures (simple way),

9

CO2

29

percentiles, simple linear correlation and regression analysis. Analysis

of variance.

3

Probability and Distributions: Sample space, types of event, definition,

axioms and classical definition of probability, addition and multiplication

rules of probability and Bayes rule, conditional probability, random

variable for continuous and discrete probability, probability distributions

Binomial , Poisson and Exponential , Normal distributions and Genetic

Applications of Probability, Hardy - Weinberg law

9 CO3

4

Statistical inference: Estimation: Standard error, confidence interval

for means and proportion. Testing of hypothesis: basic concepts, types

of errors. Test of significance for small and large samples; student t-

distribution, F- distribution, Chi -Square distribution, goodness of fit

test. Analysis of variance (one-way and two-way classifications).

9 CO4

5

Statistical modeling in Biology: Microbial Growth in a Chemostat and

turbidostat, Growth Equations of Microbial populations, Models of

Commensalisms, Mutualism, Predation and Mutation. Volterra’s Model

for n -interacting Species. Basic Models for Inheritance, Selection and

Mutation Models, Genetic Inbreeding Models.

9 CO5

Text Books:

1. Fundamentals Of Biostatistics, Rastogi V. B., Ane Books Pvt Ltd, 2nd Edition, 2009, ISBN: 8180522555, 9788180522550.

2. An Introduction to Biostatistics, N Gurumani, MJP Publisher, 1st Edition, 2008, ISBN: 8180942562, 9788180942563.

Reference Books:

1. Principles of Biostatistics, Marcello Pagano and Kimberlee Gauvreau, Thompson Learning, 2nd edition, 2004, ISBN: 0534229026, 9780534229023.

2. Fundamentals of Biostatistics, Bernard Rosner, , Cengage Learning illustrated, revised, 7th edition, 2010, ISBN: 0538733497, 9780538733496.

30

3. An Introduction to Biostatistics, Thomas Glover, Kevin Mitchell, Waveland Press, 3rd Edition, 2015, ISBN: 1478631112, 9781478631118.

4. Introduction to Biostatistics & Computer Science, Mr. Y. I. Shah Dr. A. R. Paradkar, Mr. M. G. Dhayagude, Pragati Books Pvt. Ltd., 1st Edition, 2008, ISBN: 8185790337, 9788185790336.

Assessment Pattern CIE- Continuous Internal Evaluation (50 Marks)

Bloom’s Taxonomy

Tests (25 Marks)

Assignments (10 Marks )

Quizzes (5 Marks )

Curricular / Cocurricular (10 Marks)

Remember 5 3 2 - Understand 5 2 2 5

Apply 5 2 1 5 Analyze 5 2 - - Evaluate 5 1 - - Create - - - -

SEE- Semester End Examination (50 Marks)

Bloom’s Category Test (50 Marks)

Marks(Out of 50) Remember 10 Understand 10

Apply 20 Analyze 5 Evaluate 5 Create -

31

MICROBIOLOGY

Course Code : BTE43 Credits : 05 L:P:T:S : 3:2:0:0 CIE Marks : 50+25 Exam Hours : 3+3 SEE Marks : 50+25 Course Outcomes: At the end of the Course, the Student will be able to: CO1 To understand the different types of microorganisms, their classification, modes of

reproduction and significance & hands-on with basic research procedure for identification of unknown microorganism specimen.

CO2 To learn the role of microorganisms in biofertilisers, food and industrial application etc. CO3 To evaluate and apply the proper methods of microbial control. CO4 To develop hands on laboratory skills with an understanding of important facts, concepts,

and the investigative procedures of microbiological techniques. CO5 To understand the different types of media, growth parameters and cultural conditions

for microorganisms. CO6 To learn the role of microorganisms in pathogenicity & diseases & train students in

aseptic techniques, and the methods relating to the safe handling & maintenance of microorganism.

Mapping of Course Outcomes to Program Outcomes: PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12 CO1 2 3 2 - 3 2 2 - 3 2 2 - CO2 2 2 3 2 2 - 2 2 2 - 3 1 CO3 3 3 3 - 2 1 2 - 2 2 3 1 CO4 3 2 3 3 3 1 - - 2 1 2 1 CO5 3 3 2 3 3 - - - 2 - 3 1 CO6 2 1 3 2 3 1 3 2 2 1 2 3 Module No

Module Contents Hours COs

1

Introduction: The Scope of Microbiology and role of Microbiologist, History of Microbiology, Microbial diversity and Taxonomy, Types of Microorganisms - Bacteria, Viruses, Fungi, Protozoa, Algae - their Structure, Classification, Modes of Reproduction & Significance.

9

CO1 List of Experiments

1. Microscopic observation of Prokaryotic cells (bacteria). 2. Microscopic observation of Eukaryotic cells (fungi,

yeasts and moulds).

8

2 Methods in Microbiology: Basic principles of Microscopy, CO4

32

Bright-Field Microscopy, Dark-Field Microscopy, Phase-Contrast Microscopy, Fluorescence Microscopy, Electron Microscopy – SEM, TEM. Pure culture Techniques (streak-plate, spread plate, pour plate), Cultural characteristics, staining of Microorganisms (Simple staining, differential staining), types of stains, theories of staining, Enumeration of Microorganisms (Direct Microscopic Count, by Pour plate technique, Streak plate, membrane filtration, Electronic Methods, etc)

9

List of Experiments 1. Isolation of bacteria by serial dilution, pour plate and

streak plate techniques. 2. Enumeration of total count (haemocytometer) and viable

count of bacteria. 3. Identification of bacteria by size, shape, Gram and

Endospore staining.

8

3

Microorganisms- Bacteria: The morphology and fine structure of Bacteria, Cultivation of Bacteria, Media and types of media Nutritional requirements, Reproduction and Growth, Factors affecting growth, Measurement of growth. Microbial Metabolism: Aerobic and anaerobic growth. Biochemical tests.

9

CO3, CO5 List of Experiments 1. Growth curve of bacteria. 2. Biochemical tests - IMViC tests, Starch hydrolysis, Catalase

activity.

8

4

Control of Microorganisms: Control of Microorganisms by physical methods (heat, filtration, radiation), Control by chemical methods (phenol & phenolic compounds, Alcohols, Halogens, Dyes, Detergents, Aldehydes, Heavy metals, etc), Antibiotics and other chemotherapeutic agents.

9

CO3

List of Experiments 1. Antibiotic susceptibility testing of a bacterium. 2. Media preparation, plugging and sterilization.

8

5

Applications of Microbiology: Microbiology of soil, Biofertilizers, Microbes in Bioremediation, Traditional microbial processes using yeasts and bacteria, Microbes as source of protein, Microbial Insecticides, Enzymes from microbes, primary and secondary metabolites. Clinical Microbiology. Pathogenesis of microorganisms, Human diseases caused by microbes viruses (HIV), bacteria (TB, Cholera and E.coli), Protozoans (Malaria and amebiasis).

9

CO2, CO6

List of Experiments 1. Isolation, culturing and identification of microorganisms

from air, water & soil. 2. Alcoholic and mixed acid fermentation (Wine, Lactic acid).

8

33

Text Books: 1. Microbiology, Lansing M. Prescott, John P. Harley, Donald A. Klein, McGraw- Hill

Publications, 6th Edition, 2005, ISBN: 0072951753, 9780072951752. 2. Textbook of Microbiology, Ananthanarayan And Paniker, Reprint. Orient Blackswan, 7th

Edition, 2006, ISBN: 8125028080, 9788125028086.

Reference Books:

1. Microbiology: Principles and Explorations, Jacquelyn G. Black, Laura J. Black, Wiley, 9th Edition, 2014, ISBN: 111893475X, 9781118934753.

2. Microbial Biotechnology: Fundamentals of Applied Microbiology, Alexander N. Glazer, Hiroshi Nikaido, Cambridge University Press, 2nd Edition, 2007, ISBN: 1139465635, 9781139465632.

3. Microbiology: An Introduction, Tortora GJ, Funke BR, and Case CL, Benjamin-Cummings Pub Co, 4th Edition, 2005, ISBN: 8125028080, 9788125028086.

4. Experiments in Microbiology, Plant Pathology and Biotechnology, K. R. Aneja, New Age International, 4th Edition, 2003, ISBN: 812241494X, 9788122414943.

Assessment Pattern CIE- Continuous Internal Evaluation: Theory (50 Marks)

Bloom’s Taxonomy

Tests (25 Marks)

Assignments (10 Marks )

Quizzes (5 Marks )

Curricular / Cocurricular (10 Marks)

Remember 10 5 2 - Understand 10 5 2 5

Apply 5 - 1 5 Analyze - - - - Evaluate - - - - Create - - - -

34

CIE- Continuous Internal Evaluation: Lab (25 Marks)

Bloom’s Category Lab Marks (out of 25)

Remember - Understand -

Apply 10 Analyze 10 Evaluate 5 Create -

SEE- Semester End Examination: Theory (50 Marks) Bloom’s Category

Tests Marks(Out of 50) Remember 30 Understand 10 Apply 10 Analyze - Evaluate - Create - SEE- Semester End Examination: Lab (25 Marks)

Bloom’s Category Lab Marks(Out of 25) Remember - Understand - Apply 10 Analyze 10 Evaluate 5 Create -

35

MOLECULAR BIOLOGY

Course Code : BTE44 Credits: 05 L:P:T:S : 3:2:0:0 CIE Marks: 50+25 Exam Hours : 3+3 SEE Marks: 50+25 Course Outcomes: At the end of the Course, the Student will be able to: CO1 Portray the knowledge of molecular events in central dogma of life by virtue of strong

fundamentals in nucleic acid biology CO2 Identify the principles of DNA replication, transcription and translation and explain how

they relate to each other CO3 Discuss clearly about gene organization, expression and regulation in both prokaryotic

and eukaryotic organisms CO4 Understand the mechanism of recombination and apply the knowledge for benefit of

mankind CO5 Articulate various molecular solutions with broad applications in the area of

Biotechnology CO6 Able to demonstrate knowledge and understandings of the principles of various

molecular biology techniques Mapping of Course Outcomes to Program Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12 CO1 3 2 2 2 1 2 1 2 3 2 1 - CO2 3 3 2 - - - - - - 1 1 - CO3 3 - - - - - - - - 1 2 - CO4 3 2 2 2 2 3 - 2 3 3 3 - CO5 3 3 3 3 3 3 2 2 3 2 3 1 CO6 3 3 3 3 3 3 3 3 3 2 3 2

Module No

Module Contents Hours COs

1

Fundamentals of molecular biology and DNA replication: Introduction and scope of molecular biology. Information flow in biological systems: Central dogma, updated central dogma. Forms of nucleic acids–DNA and RNA. DNA super coiling. DNA Replication-Meselson & Stahl experiment, Replication in prokaryotes: prokaryotic DNA polymerases, mechanism of DNA replication and different models. Replication in Eukaryotes-

9 CO1

36

Eukaryotic DNA polymerases, mechanism of DNA replication, Fidelity of DNA replication: Inhibitors of DNA replication. PCR. List of Experiments

1. Preparation of genomic DNA (Bacterial or Plant sources – any one).

2. Checking the purity and quantification of DNA by UV spectrophotometric method.

3. Specific amplification of DNA by means of PCR technology.

4. DNA viability test.

8

2

Transcription: Structural organization of prokaryotic and eukaryotic chromosomal genes and their sequences. Plasmid. Transcription apparatus & Mechanism: Structure and functions of both prokaryotic (bacterial) and eukaryotic RNA polymerases, Transcription factors, mechanism of transcription in prokaryotes and eukaryotes. Fidelity of RNA synthesis, Posttranscriptional modification: 5’ end capping, 3’ end poly A tailing, mechanism of splicing (type-I, type II and - Spliceosome mediated), alternative splicing and its biological importance and RNA editing. Inhibitors of transcription.

9

CO1, CO2

List of Experiments 1. Isolation of Plasmid DNA from Bacterial source by

alkaline lyses method. 2. Agarose Gel Electrophoresis of DNA (Genomic DNA and

Plasmid DNA) 3. Restriction enzyme digestion of plasmid DNA.

8

3

Translation: Introduction to Genetic code: Elucidation of genetic code, Codon degeneracy, Wobble hypothesis and its importance, Prokaryotic and eukaryotic ribosomes. Components of translation. Activation of tRNA. Mechanism of translation: Initiation, Elongation and Termination of protein synthesis, Differences between prokaryotic and eukaryotic protein synthesis. Post- translational modifications and its importance. protein splicing. protein targeting: signal hypothesis and cotranslational processing, transportation. Inhibitors of protein synthesis.

9

CO3, CO5

List of Experiments 1. Extraction and estimation of total seed protein 2. Separation of proteins by electrophoresis (SDS- PAGE)

8

4 REGULATION OF GENE EXPRESSION: Hierarchical levels of gene regulation, Regulation of gene expression in prokaryotes: Operon model: Basic concept and significance, Structure and

9 CO4

37

regulation of gal, lac and trp operons; positive versus negative regulation. Regulation of gene expression in eukaryotes: transcriptional control, homeobox in the control of developments in insects and vertebrates. Gene silencing: anti-sense RNA, RNAi. Posttranslational regulation, protein degradation and turnover. List of Experiments

1. Isolation of total RNA (Bacterial or Plant sources). 2. Estimation of RNA by Orcinol method.

6

5

Genetic recombination and DNA repair: Genetic recombination in bacteria and viruses, site-specific recombination, Transposable genetic elements (TE): History and Introduction, classification, Insertion sequences in bacteria, mobile elements of maize (McClintock’s work), Mechanism of transposition, role in disease induction and silencing of TEs, retroposons. DNA damage and repair: Source and types of DNA damage, Various types of DNA repair mechanisms, DAN repair and cancer.

9

CO6

List of Experiments 1. Transformation of E. coli cells – Calcium chloride

method. 2. Selection of recombinants (Blue-white screening). 3. Bacterial Conjugation process

10

Text books:

1. Karp's Cell and Molecular Biology: Concepts and Experiments, Gerald Karp, Janet Iwasa, Wallace Marshall, Wiley, 8th Edition, 2016, ISBN: 1118883810, 9781118883815.

2. The Condensed Protocols from Molecular Cloning: A Laboratory Manual, Joseph Sambrook, David William Russell, CSHL Press, Illustrated Edition, 2006, ISBN: 0879697717, 9780879697716.

References:

1. Molecular Biology of the Cell, Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter, Garland Science, Sixth Edition, , 2014, ISBN: 1317563751, 9781317563754.

2. Lewin's GENES XI, Jocelyn E. Krebs, Benjamin Lewin, Elliott S. Goldstein, Stephen T. Kilpatrick, Jones & Bartlett Publishers, 2014, ISBN: 1449659055, 9781449659059.

3. Molecular Biotechnology: Principles and Applications of Recombinant DNA, Bernard R. Glick, Jack J. Pasternak, ASM Press, Digital Edition. 2nd edition, 2010, ISBN: 1555811361, 9781555811365.

38

4. Recombinant DNA, James D. Watson, W. H. Freeman, Illustrated Edition, 1992, ISBN: 0716722828, 9780716722823.

Assessment Pattern CIE- Continuous Internal Evaluation: Theory (50 Marks)

Bloom’s Taxonomy

Tests (25 Marks)

Assignments (10 Marks )

Quizzes (5 Marks )

Curricular / Cocurricular (10 Marks)

Remember 10 - 2 - Understand 10 5 2 5

Apply 5 5 1 5 Analyze - - - - Evaluate - - - - Create - - - -

CIE- Continuous Internal Evaluation: Lab (25 Marks)

Bloom’s Category Lab Marks (out of 25)

Remember - Understand 3

Apply 10 Analyze 7 Evaluate 5 Create -

SEE- Semester End Examination: Theory (50 Marks) Bloom’s Category Tests Marks(Out of 50) Remember 10 Understand 7 Apply 20 Analyze 8 Evaluate 5 Create -

39

SEE- Semester End Examination: Lab (25 Marks) Bloom’s Category Lab Marks(Out of 25) Remember - Understand 3 Apply 10 Analyze 7 Evaluate 5 Create -

BIOCHEMICAL THERMODYNAMICS

Course Code : BTE45 Credits : 05 L:P:T:S : 3:2:0:0 CIE Marks : 50+25 Exam Hours : 3+3 SEE Marks : 50+25

Course Outcomes: At the end of the Course, the Student will be able to:

CO1 Understand the principles and basic concepts of thermodynamics

CO2 Learn the laws governing thermodynamics and apply to understand the energy in transformation

CO3 Apply laws of Thermodynamics to learn heat engines and heat pumps.

CO4 Learn fluid property relations and their deviations from ideality

CO5 Develop practical skills for solving Thermodynamics related problems

CO6 Incorporate Skills developed to apply Thermodynamics for biological systems

Mapping of Course Outcomes to Program Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 2 1 1 1 - - - - - 1 -

CO2 3 2 1 1 - - - - - - 1 -

CO3 2 2 - 1 - - - - - - - -

40

CO4 1 1 - - - - - - - - 1 -

CO5 1 3 3 3 1 - - - 1 - 1 1

CO6 1 2 3 2 1 2 2 1 1 - 1 1

Module No Module Contents Hours COs

1

Introduction: Thermodynamics and Scope of Thermodynamics, Dimensions and units, Measures of amount of substance, force, temperature, pressure, work, energy and heat. System, surrounding & processes, closed and open systems, intensive & extensive properties, state and path functions, equilibrium state, Internal energy, reversible and irreversible processes. The First Law and other basic concepts: Joules experiments, First Law of Thermodynamics: General statement of first law of thermodynamics, first law for cyclic process, Non-flow process, flow process.

9

CO1,CO5

List of Experiments 1. Determination of Specific heat of water sample using bomb calorimeter. 2. Determination of work done by gas during Isothermal compression.(application of first law) 3. Determination of work done by gas during Isothermal Expansion(Application of first law)

8

2

The Second Law of Thermodynamics: Statement, heat engines, heat pumps, and Thermodynamic temperature scales, Entropy, entropy changes for ideal gas, mathematical statement for second law: Clausius and Kelvins inequality, Entropy balances for open systems, Calculation of ideal work, lost work, The third law of thermodynamics. Thermodynamic properties of fluids: Property relations, residual properties, residual properties by equations of state, two phase systems, thermodynamic diagrams.

9 CO2,CO3,CO5

List of Experiments 1. Determination of efficiency of a heat Engine (application of second law) 2. Determination of coefficient of performance of heat pump (Application of second law).

8

3

Vapor Liquid Equilibrium: P-V-T behaviour of pure fluids, equations of state and ideal gas law, processes involving ideal gas law: constant volume, constant pressure, constant temperature, adiabatic and polytropic processes. Equations of real gases, principles of corresponding states, compressibility charts.

9 CO4, CO5

41

Text Books:

1. Fundamentals of Chemical Engineering Thermodynamics, Themis Matsoukas, Prentice Hall International Series in the Physical and Chemical Engineering Sciences, Prentice Hall, 2012, ISBN: 0132693186, 9780132693189.

2. A Textbook of Chemical Engineering Thermodynamics, Narayanan KV, Prentice Hall Publication, 2nd Edition, 2013, ISBN: 8120347471, 9788120347472.

Solution Thermodynamics: Fundamental property relation, The chemical potential and phase equilibrium, partial properties, fugacity and fugacity coefficient: Pure species, species in solution, generalized correlation for the fugacity coefficient, Ideal solution model, excess properties.

List of Experiments 1. Verification of Ideal gas law (Boyles law and Charles law) 2. Study of heat transfer by conduction.

8

4

Properties of Solutions & Phase Equilibria: Partial molar properties, Chemical potential, Gibbs-Duhem equation & its applications, Henry’s law &Raoult’s law. Criteria of phase Equilibria, criterion of stability, Duhem's theorem, Vapour- Liquid Equilibria: VLE in ideal solutions, Consistency test for VLE data, calculation of activity coefficients using Gibbs - Duhem equation, Liquid-Liquid Equilibrium diagrams Heat Effects: Sensible Heat effects, latent heat of pure substances, standard heat of reaction, formation, combustion, temperature dependence of Enthalpy

9

CO1,CO3

List of Experiments 1. Determination of latent heat of fusion and latent heat of vaporization 2. Study of heat transfer by convection 3. Study of heat transfer by radiation

8

5

Biochemical Energetics: Coupled reactions, Reaction Stoichiometry, criteria of biochemical reaction equilibrium, equilibrium constant and standard free energy change, effect of temperature, pressure on equilibrium constants and other- factors affecting equilibrium conversion. Biochemical Thermodynamics: Acidity of solutions, Ionisation of biochemicals, solubility’s of weak acids and weak bases. Protein concentration in an ultra centrifuge, Gibbs Donnan equilibrium and membrane potentials, ATP-ADP energy storage mechanism, Thermodynamic analysis of fermenters.

9

CO6

List of Experiments 1. Determination of Thermal conductivity of a organic samples. 2. Determination of calorific value of biomass/food. 3. Determination of moisture content in a given sample.

8

42

Reference books:

1. Bioprocess Engineering: Basic Concepts, Shuler ML and Kargi F, Pearson, 2nd Edition, 2014 , ISBN: 1292025999, 9781292025995.

2. Biochemical Calculations, Irwin Segel, 2nd Edition, John Wiley & Sons , 2008 (digital), ISBN: 0471774219, 9780471774211.

3. Thermodynamics of Biochemical Reactions, Robert A. Alberty, John Wiley & Sons, 2005, ISBN: 0471623555, 9780471623557.

4. Introduction to chemical engineering thermodynamics, Joseph Mauk Smith, Hendrick C. Van Ness, Michael M. Abbott, , illustrated, McGraw-Hill, Edn. 7, 2005, ISBN: 0073104450, 9780073104454.

Assessment Pattern CIE- Continuous Internal Evaluation: Theory (50 Marks)

Bloom’s Taxonomy

Tests (25 Marks)

Assignments (10 Marks )

Quizzes (5 Marks )

Curricular / Cocurricular (10 Marks)

Remember 5 2 2 - Understand 5 2 2 5

Apply 10 2 1 5 Analyze 5 2 - - Evaluate - 2 - - Create - - - -

CIE- Continuous Internal Evaluation: Lab (25 Marks)

Bloom’s Category Lab Marks(Out of 25) Remember 5 Understand 5 Apply 10 Analyze 3 Evaluate 2 Create -

SEE- Semester End Examination: Theory (50 Marks)

Bloom’s Category Tests Marks(Out of 50) Remember 20 Understand 10

43

Apply 15 Analyze 3 Evaluate 2 Create -

SEE- Semester End Examination: Lab (25 Marks)

Bloom’s Category Lab Marks (out of 25)

Remember 5 Understand 5

Apply 10 Analyze 3 Evaluate 2 Create -

BIOPHYSICS & BIOANALYTICAL TECHNIQUES

Course Code : BTE46 Credits : 05 L:P:T:S : 3:0:1:1 CIE Marks : 50 Exam Hours : 03 SEE Marks : 50 Course Outcomes: At the end of the Course, the Student will be able to: CO1 Understand the underlying principles, instrumentation and applications of various

bioanalytical techniques CO2 Understand the analytical techniques for isolation and purification of biomolecules CO3 Apply and analyze the techniques based on the spectral characteristics of the

biomolecules CO4 Know the techniques for diagnosis and detection of diseases using modern analytical

tools. CO5 Apply the developed knowledge for trouble shooting the problems real time. CO6 Give ecofriendly solutions for scale up operations in bioindustries. Mapping of Course Outcomes to Program Outcomes: PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12 CO1 3 2 - - - - - - - - 1 - CO2 3 1 - - - - - - - - 1 - CO3 1 - - - - - - - - - 1 -

44

CO4 1 2 1 2 3 2 - 2 - 1 3 - CO5 1 3 1 2 2 1 - - 2 1 - 1 CO6 3 1 3 3 2 1 3 - 2 1 - 2 Module No

Module Contents Hours COs

1

Introduction to electromagnetic spectrum. Optical Activity, CD, UV, IR and Laser Raman. Principles, instrumentation and applications of Spectrophotometric techniques, Mass spectrometry, LCMS, MALDI-TOF, X-ray diffraction: structure determination via single crystal diffraction, fibre diffraction, XAFS, NMR spectroscopy, ESR/EPR.

9

CO1 CO2

2

Centrifugation: Basic principles of sedimentation, Forces involved – RCF, Sedimentation coefficient, factors affecting sedimentation coefficient. Types of Centrifuges. Working principle and applications of preparative [Differential, Density Gradient] and analytical [sedimentation velocity, sedimentation equilibrium] ultracentrifugation.

9

CO1 CO3

3

Chromatography: Principles of chromatography - Partition coefficient. Basic principle, instrumentation and applications of Gel filtration, Affinity, Column, Ion-exchange, Gas – Liquid and High Pressure liquid chromatography – HPTLC.

9 CO1 CO4

4

Electrophoresis: Principle of electrophoresis, Factors affecting electrophoretic mobility. Support media. Basic principle, instrumentation and applications of electrophoretic techniques for proteins (PAGE, SDS PAGE, IEF, 2D gel electrophoresis) and nucleic acids (Agarose gel electrophoresis, PFGE). Blotting techniques.

9

CO1 CO5

5

Radioisotope techniques: Radioactivity, Ionizing and Nonionizing Radiation Isotopes in Biochemistry. Detection and measurement of radioactivity. Application of Isotopes. Safety precautions for using radioactive isotopes. Flow Cytometry.

9 CO1 CO6

Text Books:

1. Principles and techniques of biochemistry and molecular biology, Wilson K, Walker J, editors, Cambridge university press; 7th Edition, 2010, ISBN: 0521516358, 9780521516358.

45

2. Biophysical Techniques, Iain Campbell, OUP Oxford, Illustrated Edition, 2012, ISBN: 0199642141, 9780199642144.

Reference Books:

1. Harper’s Illustrated Biochemistry, Robert K Murray, Daryl K Granner, Peter A Mayes, McGraw Hill Book Company, 29th Edn. 2012, ISBN: 0071809694, 9780071809696.

2. Fundamentals of Biochemistry: Life at the Molecular Level, Donald J. Voet, Judith G. Voet, Charlotte W. Pratt, John Wiley & Sons, 4th Edition, 2013, ISBN: 0470547847, 9780470547847.

3. Textbook of Structural Biology, Anders Liljas, World Scientific, 2009, ISBN: 9812772073, 9789812772077.

4. Bioanalytical Techniques, The Energy and Resources Institute (TERI), Abhilasha Shourie, 2005, ISBN: 8179935299, 9788179935293.

Assessment Pattern CIE- Continuous Internal Evaluation (50 Marks)

Bloom’s Taxonomy

Tests (25 Marks)

Assignments (10 Marks )

Quizzes (5 Marks )

SSA (10 Marks)

Remember 10 - 2 2 Understand 10 4 2 2

Apply 5 4 1 2 Analyze - 2 - 2 Evaluate - - - 2 Create - - - -

SEE- Semester End Examination (50 Marks) Bloom’s Category Tests Marks(Out of 50) Remember 20 Understand 10 Apply 10 Analyze 10 Evaluate - Create -

46

ECONOMICS FOR ENGINEERS

Course Code : 17HSS321/421 Credits: 03

L:P:T:S : 2:0:0:1 CIE : 50

Exam Hours : 03 SEE : 50

Course Outcomes: On completion of the course, the student will be able to:

CO1 Gain knowledge about importance of economics in decision making processes in day to day life.

CO2 Analyze business environment at micro and macroeconomic level and its impact on industries in country’s economy.

CO3 Acquire knowledge about costing and estimation of projects for profit making. CO4 Apply principles of budgeting and finance for entrepreneurial success.

CO PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12 CO1 2 2 1 - 1 - 1 2 2 - 2 2 CO2 2 2 1 - 1 - 1 2 2 - 2 2 CO3 2 2 1 - 1 - 1 2 2 - 2 2 CO4 2 2 1 - 1 - 1 2 2 - 2 2

Module Contents of Module Hours COs I Introduction to Economics: Role of Engineer as an

Economist, Types and problem of economies, Basics of economics (GDP, National income, inflation, business cycle, fiscal and monetary policies, balance of payment).

4 1,3

II Basic concepts of Microeconomics: concept of Demand & Elasticity of Demand. Concept of Supply & Elasticity of Supply, Meaning of Production and factors of production, Production Possibility Curve, Law of variable proportions and returns to scale. Relevance of Depreciation towards industry, Depreciation computing methods.

5 2,3

III Concepts of cost of production: different types of cost; accounting cost, sunk cost, marginal cost and opportunity cost. Break even analysis, Make or Buy decision. Cost estimation, Elements of cost as Direct Material Costs, Direct Labor Costs, Fixed Over-Heads, Factory cost, Administrative Over-Heads.

4 3,4

IV Capital budgeting: Traditional and modern methods, 4 1,3,4

47

Payback period method, IRR, ARR, NPV, PI. . Interest and Interest factors: Interest rate, Simple interest, Compound interest, Cash - flow diagrams, Personal loansand EMI Payment. Present worth, Future worth.

V Book Keeping and Accounts: Journal, Ledger, Trial balance, asset Types, profit & loss account, balance sheet.

5 1,2,3,4

TEXT BOOKS: 1. Riggs J.L, Engineering Economy, TMH, 2012 edition 2. Jain T.R., Economics for Engineers, VK Publications 3. IM PANDEY,Finacial Management, Vikas Pub. House 4. D N Dwivedi, Mangerial Economics , Vikas Pub. House REFERENCE BOOKS: 1.Thuesen H.G, Engineering Economy. PHI 2. Prasanna Chandra, Financial Mangement,TMH 3. Singh Seema, Economics for Engineers, IK International 4. Chopra P. N, Principle of Economics, Kalyani Publishers 5. Dewett K K, Modern Economic Theory, S. Chand 6. H. L. Ahuja, Modern Economic Theory, S. Chand 7. Mishra S. K, Modern Micro Economics, Pragathi Publications 8. Gupta Shasi K, Management Accounting, Kalyani Publications

Assessment pattern

CIE –Continuous Internal Evaluation (50 Marks, Theory)

Bloom’s category Test Assignments SSR Marks (out of 50) 20 15 15 Remember 5 Understand 5 Apply 5 Analyze 5 5 5 Evaluate 5 5 Create 5 5

SEE –Semester Ending Examination (50 Marks)

Bloom’s category SEE Theory(50) Remember 20 Understand 10

48

Apply 10 Analyze 10 Evaluate Create

LIFE SKILLS FOR ENGINEERS

Course Code : HSS322/ HSS422 Credits : 03

L:P:T:S : 2:0:0:1 CIE Marks : 50

Exam Hours : 03 SEE Marks : 50

Course Outcomes: At the end of the Course, the Student will be able to: CO1 To transform as stronger individuals to handle life challenges of professional life. CO2 To apply the concept of Personality development & Grooming in real life. CO3 Understand the concept of self and Creativity so that they can align with their life

better. CO4 To understand the role of motivation and leadership on behavior CO5 To enhance holistic development of personality to equip the student with employability

skills. CO6 Determine the significance of goal setting & decision making in their professional life Mapping of Course Outcomes to Program Outcomes: PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 2 3 3 3 3 2 3 3 1 2 2 3 CO2 1 3 3 2 3 3 2 3 3 2 3 2 CO3 2 3 2 1 2 3 3 3 1 3 2 3 CO4 2 3 3 2 1 3 3 3 2 3 3 3 CO5 1 3 3 2 3 2 3 2 2 3 3 3 CO6 2 2 3 1 2 2 3 2 2 3 3 3

Module No Module Contents Hours COs

1 Personality development & Grooming: Expectations from the industry & Career Planning / Reality Check; Building personal presence; Corporate grooming; Corporate etiquettes; Developing personal work code.

6 CO1

2 Self Analysis & Creativity: SWOC analysis, Who am I attributes, CO2,

49

Importance of Self Confidence, Self Awareness, Self Management, Social Awareness, Emotional Intelligence, out of box thinking, lateral Thinking & Johari windows.

4 CO3

3 Motivation & Leadership: Basic concepts & theories, factor, types of Motivation, Good Leadership skill, Traits of a leader & Assessment of Leadership Skill.

4 CO4, CO5

4

Interpersonal Skill: Assessment of interpersonal skills, situation detail of interpersonal skill, Team Working, leading a team, and Strategies for influencing people. Understanding the relationship among motivation, leadership and team work

4 CO5

5 Goal Setting and Decision Making: identifying goals like (short term, long term, lifetime goals), Time management, importance of work scheduling, importance and necessity of decision making.

4 CO6

Text Books:

1) Soft Skill, 2015, Career development Centre, Green Pearl Publication. Reference Books:

1) The 7 – Habits of Highly Effective People, Stephen R Covey, Neha Publishers. 2) Seven Habits of Highly Effective Teens, Convey Sean, New York, Fireside Publishers,

1998. 3) Emotional Intelligence, Daniel Coleman, Bantam Book, 2006.

Assessment Pattern CIE- Continuous Internal Evaluation (50 Marks)

SEE- Semester End Examination (50 Marks) Bloom’s Category Tests Marks (Out of 50) Remember 5 Understand 10 Apply 15 Analyze 10 Evaluate 5 Create 5

Bloom’s Category Tests Assignments Quizzes Marks (out of 50) 30 10 10 Remember - - 5 Understand 5 - 5 Apply 10 5 - Analyze 5 - - Evaluate 5 - - Create 5 5 -

50

APPENDIX A

Outcome Based Education

Outcome-based education (OBE) is an educational theory that bases each part of an educational system around goals (outcomes). By the end of the educational experience each student should have achieved the goal. There is no specified style of teaching or assessment in OBE; instead classes, opportunities, and assessments should all help students achieve the specified outcomes.

There are three educational Outcomes as defined by the National Board of Accredition:

Program Educational Objectives: The Educational objectives of an engineering degree program are the statements that describe the expected achievements of graduate in their career and also in particular what the graduates are expected to perform and achieve during the first few years after graduation. [nbaindia.org]

Program Outcomes: What the student would demonstrate upon graduation. Graduate attributes are separately listed in Appendix C

Course Outcome: The specific outcome/s of each course/subject that is a part of the program curriculum. Each subject/course is expected to have a set of Course Outcomes

Mapping of Outcomes

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APPENDIX B The Graduate Attributes of NBA

Engineering knowledge: Apply the knowledge of mathematics, science, engineering fundamentals, and an engineering specialisation to the solution of complex engineering problems. Problem analysis: Identify, formulate, research literature, and analyse complex engineering problems reaching substantiated conclusions using first principles of mathematics, natural sciences, and engineering sciences. Design/development of solutions: Design solutions for complex engineering problems and design system components or processes that meet the specified needs with appropriate consideration for the public health and safety, and the cultural, societal, and environmental considerations. Conduct investigations of complex problems: The problems that cannot be solved by straightforward application of knowledge, theories and techniques applicable to the engineering discipline that may not have a unique solution. For example, a design problem can be solved in many ways and lead to multiple possible solutions that require consideration of appropriate constraints/requirements not explicitly given in the problem statement (like: cost, power requirement, durability, product life, etc.) which need to be defined (modeled) within appropriate mathematical framework that often require use of modern computational concepts and tools. Modern tool usage: Create, select, and apply appropriate techniques, resources, and modern engineering and IT tools including prediction and modelling to complex engineering activities with an understanding of the limitations. The engineer and society: Apply reasoning informed by the contextual knowledge to assess societal, health, safety, legal, and cultural issues and the consequent responsibilities relevant to the professional engineering practice. Environment and sustainability: Understand the impact of the professional engineering solutions in societal and environmental contexts, and demonstrate the knowledge of, and need for sustainable development. Ethics: Apply ethical principles and commit to professional ethics and responsibilities and norms of the engineering practice. Individual and team work: Function effectively as an individual, and as a member or leader in diverse teams, and in multidisciplinary settings. Communication: Communicate effectively on complex engineering activities with the engineering community and with society at large, such as, being able to comprehend and write effective reports and design documentation, make effective presentations, and give and receive clear instructions. Project management and finance: Demonstrate knowledge and understanding of the engineering and management principles and apply these to one’s own work, as a member and leader in a team, to manage projects and in multidisciplinary environments. Life-long learning: Recognise the need for, and have the preparation and ability to engage in independent and life-long learning in the broadest context of technological change.

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APPENDIX C

BLOOM’S TAXONOMY

Bloom’s taxonomy is a classification system used to define and distinguish different levels of human cognition—i.e., thinking, learning, and understanding. Educators have typically used Bloom’s taxonomy to inform or guide the development of assessments (tests and other evaluations of student learning), curriculum (units, lessons, projects, and other learning activities), and instructional methods such as questioning strategies. [eduglosarry.org]