University of Puerto Rico - CayeyRISE Program

Rubric for Assessing Laboratory Summaries

Name 6. Amanda M. Icazatti Burtell Points

Date February 6th, 2014 30 Points Total 23.5/30Summary Number 2

1 2 3 4 5A. PURPOSE OF THE LAB TECHNIQUE

Refers to purpose and objectives of the lab techniques XB. BIOLOGICAL COMPETENCE

Demonstrates knowledge of laboratory procedures x

Reports findings adequately xC. ENGLISH COMPETENCE

Uses correct grammar, syntax, spelling, and punctuation 4.5

Demonstrates clarity and coherence xD. CRITICAL THINKING

In concluding identifies applications and or implications of the techniques

x

Template for 2nd Workshop Summary Biol. 4997-Biomedical Techniques Due date February 7, 2014

2nd Summary: Microscopy and Photomicrography January 26, 2014 Dr. Robert RossThe majority of the things we study are perceived by the naked eye, but there is much more than meets the eye.

Microscopes give us the opportunity to investigate and analyze about microorganisms and microscopic parts, as

cells, in much details. Through this Microscopy Workshop, we were taught on the use and characteristics of

different types of microscopies, and learned how to identify the variety of micro-techniques use in microscopy. In

this summary you are expected to demonstrate what you learned about the different types of microscopy and their

use. As part of the learning process each of the students was taught to useon a specific microscopy. Then we were

paired to serve as mentors, as a way we canto reinforced what we hadve learned. I was instructed on how to

manage a Bright-Field microscopy and educated my partner on its usethat. My partner taught me on how to use

Phase Contrast, and I was able to observed Elodea nucleus and other cell structure with it. Without any doubt,

knowing the correct way to manipulate such equipment is a very useful tool for scientists in any understanding any

process This statement is too general. It does not point to any specific and clear implication or

application..Remember word count is not that restricted for the summaries.

Word Count 163

The majority of the things we study are perceived by the naked eye, but there is much more than meets the eye. Microscopes give us the opportunity to investigate and analyze about microorganisms and microscopic parts, as cells, in much details.

There are different kinds of microscopies. Every microscopy is used for different purposes and requires a specific preparation of the specimen. Phase contrast microscope is used for living specimen without the need of applying a dye to the sample. Something that characterized this type of microscopy is the halo which the specimen would have around it. The Normaski microscope enhances the contrast of transparent samples, giving a perspective similar to the phase contrast microscopy, except that there is no halo. A Dissecting microscope is used for the examination of three dimensional specimens, rather than examining specimens in slides providing an advantage in scientific projects which requires the dissection and study of an organism and its parts. In Black field and Bright field microscope, the field around the specimen would be black and bright, respectively, which permits to contrast the unstained samples. Depending on the approach scientists want to obtain and the type of specimen being studied, different microscopy techniques can be applied. These microscopy techniques refer to the way the specimen is being cut and manipulated for the study. There are transverse, longitudinal, tangential, and cross sections that can be made to the sample.

As part of the learning process each of the students was taught to use a specific microscopy. Then we were paired to serve as mentors, as a way to reinforce what we had learned. I was instructed on how to manage a Bright-Field microscopy and educated my partner on its use. My partner taught me how to use Phase Contrast, and I was able to observed Elodea nucleus and other cell structure with it.

Knowing how to manipulate microscopes and the different techniques that are applied to samples has enriched my process as a science student because is a knowledge that can be applied to all my courses and future goals. The use of a microscopy can give scientists different perspectives on the behavior and characteristics of an organism and it represents a very useful tool that can lead to a better understanding of biological processes.