8. appendicesshodhganga.inflibnet.ac.in/bitstream/10603/71531/15/15_appendix.pdfmolecular...

Molecular Characterization of Erythrocyte Glucose- 6 -Phosphate Dehydrogenase Deficiencyin Jeddah, Saudi Arabia

Ph.D. Thesis Biotechnology 127 Kadi Sarva Vishwavidyalaya, Gandhinagar

8. APPENDICES



8. APPENDICESAppendix I: Reagents and Buffers

1. Decontamination of Concentrated Solution of Ethidium Bromide

INGREDIENTS QUANTITYEthidum Bromide 100 ml0.5M KMnO4 100ml2.5 N HCl 100mlStand at Room Temp Several hours2.5 N NaOH 100mlDiscard the solution.

2. Gel Loading Buffer

INGREDIENTS QUANTITYMgCl2 15 mMKCl 500 mMTris-HCl 100mMpH 8.3

3. 1X Phosphate Buffered Saline (PBS Buffer)

INGREDIENTS QUANTITYNaCl 8gKCl 0.2gNa2HPO4 1.44gKH2PO4 0.24gpH 7.4 with HClDistilled H2O. Adjust to 1L

4. Proteinase K buffer

INGREDIENTS QUANTITYTris-HCl 20 mMNa2EDTA 4 mMNaCl 100 mMpH 7.4

5. Red Blood Cell Lysis BufferINGREDIENTS QUANTITYSucrose 0.32 MTris HCl 10 mMMgCl2 5 mMTriton-X-100 0.75%Adjust pH to 7.6

Molecular Characterization of Erythrocyte Glucose- 6 -Phosphate Dehydrogenase Deficiency in Jeddah, Saudi Arabia

6. TE buffer (Tris-EDTA buffer)INGREDIENTS QUANTITY

Tris(hydroxymethyl) aminomethane 1 M

Diaminoethane tetraacetic acid 0.5M

Adjust pH to 8

7. Tris –Acetate Buffer (50X)INGREDIENTS QUANTITY

Tris Base 242 gGlacial Acetic acid 57.1 ml0.5M EDTA 100 mlpH 8.0Water Adjust to 1 L.




Appendix II: DEMOGRAPHIC RECORD FORM FOR G6PD DEFICIENCYPATIENTS AT KAUH, JEDDAH

A-1 Nationality: Saudi Non-Saudi

Specify: ________

Mother: Specify: ________

Father: Specify: ________

A-2 Consanguinity amongst parents: Yes NoA-3 Province: N S E W MA-4 Sex: Male FemaleA-5 Age: ________ Years ________Months Date of Birth / /

NEONATE ADULT

Yes No

B-1 Oxidative stress Causative FactorB-1 .1 InfectionB-1.2 Drug/Medicine during past weekNames of Medicines

B-1.3 Intake of FavabeansB-2 Clinical symptomsB-2.1 AHAB-2.2 Dark yellow urineB-2.3 PallorB-2.4 Abdominal painB-2.5 Neonatal JaundiceB-2.6 Infections, if anyB-3 Intervention impartedB-3.1 PhototherapyB-3.2 Blood TransfusionB-4 Associated Hemoglobinopathies

B-4.1 Thalassemia B-4.2 Sickle cell anaemia B-4.3 Sickle cell trait

C- Hematological (Complete Blood count)C-1.1 Haemoglobin C-1.2 RBC C-1.3 Reticulocytes

D- Biochemical (By Quantitative Sigma Kit)D-1 G6PD Activity U/gHbD-2 Classification (According to WHO standards)

E-1 G6PD Variant E-2 G6PD Polymorphism

D- Biochemical Estimations

Hospital Details: Ward No.: Bed No.:

Name & Contact:

A-Personal Data

C-Haematological Estimations

B-Clinical Presentations



Appendix III: Advisory Protocol for G6PD Deficient Patients.

1. Typically, an episode of drug-induced hemolysis in G-6-PD-deficient individuals

begins 1 to 3 days after drug administration is initiated. Heinz bodies appear in the red

cells, and the hemoglobin concentration begins to decline rapidly. In severe cases

abdominal or back pain may occur. The urine may turn dark – even black.

2. We advise our G6PD deficient patients to avoid following drugs

Acetanilide

Doxorubicin

Furazolidone (Furoxone)

Methylene Blue

Nalidixic acid (NeGram)

Naphthalene

Niridazole (Ambilhar)

Nitrofurantoin (Furadantin)

Phenazopyridine (Pyridium)

Phenylhydrazine

Primaquine

Sulfacetamide

Sulfamethoxazole (Gantanol)

Sulfanilamide

Sulfapyridine

Thiazolesulfone

Toluidine blue

Trinitrotoluene (TNT)

3. Fava beans and soya products be excluded from food.

4. Cocoa items be incorporated in diet.

5. Incorporation of Folic acid, Iron and Vitamin C in food / supplements

6. Immediate reporting to Hospital in case of jaundice, anemia, hemolysis, abdominal or

back pain, dark / black coloured urine or pallor.

7. Regular CBC analysis and Cytochemical observations of blood for Heinz bodies and

Haemoglobinopathies be got done periodically.

8. Medical advice be taken for the selection of antibiotics, in case of microbial or viral

Infections.

9. Immediate reporting of cases of jaundice, anemia, hemolysis, abdominal or back pain,

dark / black coloured urine or pallor to hospitals for investigations.

10. Careful vigilance on the stresses triggering / precipitating haemolysis / other G6PD

related episodes and identification of drugs and diets including broad beans, lentils and

peas as triggers.

11. Blood transfusions may be needed.

12. Dialysis may be required in cases of acute kidney injury.

13. Blood exchange transfusion may be required in cases of Infants.

14. Avoid transfusion of G6PD-deficient blood

15. Supplementation of Sn-mesoporphyrin (SnMP) a potent inhibitor of bilirubin may help in

G6PD deficiency



16. Supplementation of Pycnogenol dry extract of the French Maritime pine (Pinus pinaster)

bark that has high ability to scavenge free radicals and provide protective action against

chemical induced haemolysis in G6PD deficient human erythrocytes may be tried.

17. In case of recurrence, particularly in case of females, get the heterozygosity test

conducted and if the test is positive, such cases need vigilance and prompt care.

18. To avoid appearance of this enzymopathy in the family progenies, G6PD deficient

individuals should avoid marriage with a G6PD deficient partner.

19. Get all the Neonates, children and females of the family screened for G6PD enzyme

status at a reputed Test Centre.

20. The drugs, listed below, do not produce clinically significant hemolytic anemia and can

be given safelyin normal therapeutic doses to G-6-PD-deficient subjects. Most G-6-PD-

deficient patients have taken aspirin without untoward effect:

Appendix III: Medicines permitted for G6PD Deficient Patients.

Acetaminophen (Paracetamol, Tylenol,

Tralgon, Hydroxyacetanilide)

Acetophenetidin (phenacetin)

Acetylsalicylic acid (aspirin)

Aminopyrine (Pyramidon, amidopyrine)

Antazoline (Antistine)

Antipyrine

Ascorbic acid (vitamin C)

Benzhexol (Artane)

Chloramphenicol

Chlorguanidine (Proguanil, Paludrine)

Chloroquine

Colchicine

Diphenhydramine (Benadryl)

Isoniazid

L-Dopa

Menadione sodium bisulfite (Hykinone)

Menapthone

p -Aminobenzoic acid

Phenylbutazone

Phenytoin

Probenecid (Benemid)

Procain amide hydrochlonde (Pronestyl)

Pyrimethamine (Daraprim)

Quinidine

Quinine

Streptomycin

Sulfacytine

Sulfadiazine

Sulfaguanidine

Sulfamerazine

Sulfamethoxypyridazine (Kynex)

Sulfisoxazole (Gantrisin)

Trimethoprim

Tripelennamine (pyribenzamine)

Vitamin K



Appendix IV: Datasheet for G6PD Activity in General Samples at JeddahSample

No.Patient

IDName Sex G6PDH G6PDH G6PD

VariantU/1012RBC146-376

U/g Hb4.6-13.5

Cd-1 446567 Ameera Sheer M 55.8 1.6 MediterraneanCd-2 381990 Asma Rashid F 451.0 12.0 -Cd-3 450709 Jihan Abdul Mohaib M 348.7 11.6 -Cd-4 450548 Mariam Mohd Hababi F 19.3 0.6 AuresCd-5 443085 Hind Sabiah F 319.8 9.7 -Cd-6 447888 Fatma Mohd F 534.4 15.6 -Cd-7 403565 Abeer Fateh M 527.9 14.7 -Cd-8 342452 Nabeela Mohd F 439.9 15.3 -Cd-9 442165 Naveed Shareeff F 371.3 11.1 -

Cd-10 439976 Nawal Mohd M 361.3 10.7 -Cd-11 450784 Kalimah Mohd M 374.8 12.6 ---Cd-12 450751 Hanan Aydah M 462.8 12.2 --Cd-13 439286 Bushra Al Maki M 426.6 11.8 -Cd-14 323955 Sameera Osman M 422.6 12.3 -Cd-15 448931 Hanan Sahly F 478.4 13.8 -Cd-16 402911 Sulaiman Agrar M 477.7 23.4 -Cd-17 436270 Fatmah Saber F 388.0 12.0 -Cd-18 450824 Jumaah Saeed F 382.5 11.8 -Cd-19 378879 Samah Mohd Hameed M 510.1 17.1 -Cd-20 438033 Laila Akikah M 411.5 11.7 -Cd-21 447424 Amal Jalal F 413.2 12.6 -Cd-22 3435874 Sakeena Alazi M 522.9 18.8 -Cd-23 425967 Fatma Al Shemri F 415.2 14.8 -Cd-24 448245 Najat Ahmed F 432.6 14.3 -Cd-25 434979 Mueada Alqarni F 418.8 13.3 -Cd-26 356267 Jemma Magi F 428.1 12.2 --Cd-27 376955 Dalal Alzahrani F 402.9 14.2 -Cd-28 450844 Omal Saad Ali F 206.0 6.5 -Cd-29 383565 Khadera Jamal M 454.1 13.4 -Cd-30 413172 Marzouka Mohd F 401.8 12.0 -Cd-31 451367 Moneera Nabi Khan F 464.7 14.6 -Cd-32 310794 Safa A/Aziz F 411.9 12.5 -Cd-33 429611 Sauda Saud M 483.9 15.4 -Cd-34 445853 Kareemah Mahmood F 449.9 12.9 -Cd-35 451379 Awadi Ali Mohd M 479.1 13.1 -Cd-36 221905 Maha Fathi F 335.6 9.8 -Cd-37 330581 Raghdah Saaduddin F 459.8 12.9 -Cd-38 450176 Zamzam Abu Emam F 59.4 1.8 MediterraneanCd-39 397717 Zalikha Haso Bakhsh M 557.2 16.3 -Cd-40 450107 1.260-1.084 M 402.7 9.8 -Cd-41 448829 Shukriya Ali F 421.6 11.6 -

Contd…..



Appendix IV: Datasheet for G6PD Activity in General Samples at JeddahSampleNo.

PatientID

Name Sex G6PDH G6PDH G6PDVariantU/1012 RBC

146-376U/g Hb4.6-13.5

Cd-42 383893 Maha Ahmed F 437.2 12.6 -cd-43 359392 Bakria Mousa A M 445.7 13.5 -Cd-44 446190 Salha Saeed F 138.5 3.9 ChattamCd-45 451378 Lila Ateeq F 590.7 17.1 -Cd-46 451652 Afaf Hassan F 280.0 8.3 -Cd-47 447453 Manal Jameel M 610.0 17.3 -Cd-48 435444 Haifa A/Aziz M 420.1 11.9 -Cd-49 447909 Khadija Ahmed F 511.2 16.1 -Cd-50 449564 Samar M M 402.2 12.7 -Cd-51 451858 Fatima Mohd M 459.0 14.9 -Cd-52 450955 Ibtisam Ahmed F 364.7 10.1 -Cd-53 429878 Hafir Ahmed M 547.4 17.3 -Cd-54 446728 Latifa Mohd F 581.3 15.4 -Cd-55 281145 Maha Mahmoud F 559.7 15.7 -Cd-56 401475 Shaimah Sulaiman F 553.3 16.5 -Cd-57 450679 Mariam Baksh F 529.2 15.4 -Cd-58 436156 Fatma Atiah F 558.7 18.4 -Cd-59 276681 Azizah Ali M 681.8 20.7 -Cd-60 451134 Mona Barak F 451.4 12.7 -Cd-61 451843 Noora A F 505.2 13.4 -Cd-62 269097 Mastora Abdullah F 430.1 13.2 -Cd-63 438522 Nadia Arfat M 399.8 11.4 -Cd-64 436691 Marim Alzahrani F 432.7 13.1 -Cd-65 447883 Nazeaha Attieh M 407.4 9.2 -Cd-66 449230 Fatmah Mohd Adam M 628.7 13.4 -Cd-67 382647 Noora Mohd F 513.7 15.7 -Cd-68 453717 Fatma Al Othjaibi M 54.2 1.5 -Cd-69 396191 Huda Al -Naghi F 340.7 9.8 -Cd-70 396565 Afaf Ali M 489.5 13.6 -Cd-71 417824 Fawsia Ahmed M 348.9 10.9 -Cd-72 405592 Maimunah Awad M 530.0 14.3 -Cd-73 403788 Mariyam Wa Khider F 467.5 8.3 -Cd-74 447277 Milah Hayia M 492.9 14.5 -Cd-75 414150 Rayaah Hassan F 420.8 13.6 -Cd-76 385768 Sataah Basheer F 407.0 12.7 -Cd-77 412319 Mariam Omer F 416.2 11.4 -Cd-78 449741 Sanna Elis M 473.5 12.5 -Cd-79 450958 Huda Mohd Alamodi F 444.7 13.5 -Cd-80 411869 Ehtesah Kalid F 546.4 19.0 -Cd-81 405555 Samiah Mohd F 386.6 10.7 -Cd-82 369521 Salwa Saeed F 455.0 12.7 -

Contd…..



Appendix IV: Datasheet for G6PD Activity in General Samples at JeddahSample

No.Patient

IDName Sex G6PDH G6PDH G6PD

VariantU/1012 RBC146-376

U/g Hb4.6-13.5

Cd-83 441527 Abbasia Ahmed M 573.0 17.4 -Cd-84 384680 Fatma Ateeg M 507.0 13.3 -Cd-85 367682 Anamia Vargese M 481.6 13.9 -Cd-86 364358 Musedah Saad F 478.2 14.0 -Cd-87 391439 Najat Saad F 316.2 9.3 -Cd-88 387260 Afaf Mohd F 311.3 9.0 -Cd-89 261343 Samira Namy M 402.1 11.0 -Cd-90 444010 Suzan Mahmoud M 533.6 14.5 -Cd-91 440342 Najah Ahmed F 464.8 12.8 -Cd-92 453937 Sharifa Mohd F 399.6 11.4 -Cd-93 345055 Jumaahassan F 446.9 13.2 -Cd-94 435143 Lijal Mohd F 446.2 16.6 -Cd-95 369740 Majedah Hamed F 479.7 12.3 -Cd-96 453775 Najeah Al Hassan F 458.6 11.9 -Cd-97 440427 Entisar Alhai F 404.2 11.8 -Cd-98 440761 Zahra Abdullah M 514.0 18.6 -Cd-99 415836 Mastora Zahrani F 419.0 13.0 -

Cd-111 454181 Lubna A/Rahman M 378.5 10.7 -Cd-101 263265 Bakheeta Jubain F 573.4 16.8 -Cd-102 416847 Nahid Asaad M 436.6 13.0 -Cd-103 441955 Hanan Saleh Hassan F 415.1 11.9 -Cd-104 388878 Fatma Houd B F 665.4 19.0 -Cd-105 281998 Nabeela Abdulkarim F 453.6 12.4 -Cd-106 328621 Safia Osman M 540.8 15.6 -Cd-107 218565 Abadah Obeed F 485.8 12.5 -Cd-108 435789 Amaal Ali Al Amri F 263.4 9.7 -Cd-109 453744 Badeeah M. F 575.7 15.9 -Cd-110 451267 Nadiah Sayed M 458.0 13.4 -Cd-111 444095 Wedad Farhan F 468.7 13.3 -Cd-112 348445 Eman Omer M 555.9 16.4 -Cd-113 441187 Yosra Samih M 490.3 15.1 -Cd-114 360388 Faizah Hamraah M 447.0 12.2 -Cd-115 440877 Hadiah Awad M 515.8 13.0 -Cd-116 421161 Mardiah Mohd F 571.5 16.1 -Cd-117 439862 Maha(Baby) F 543.7 14.6 -Cd-118 278954 Latifa Saeed (Baby) M 484.9 13.1 -Cd-119 313983 Khetani Raji(Baby) M 456.6 15.9 -Cd-120 376648 Salma Nooralhuda(Baby) M 433.8 11.8 -Cd-121 340892 Noora Hassan(Baby) F 468.1 14.0 -Cd-122 454449 Taflah Bin F 446.7 12.3 -Cd-123 215207 Noora Mufiq M 562.2 17.1 -

Contd…..



Appendix IV: Datasheet for G6PD Activity in General Samples at Jeddah

SampleNo.

PatientID

Name Sex G6PDH G6PDH G6PDVariantU/1012 RBC

146-376U/g Hb4.6-13.5

Cd-124 279407 Saadah Fazey M 458.4 12.9 -Cd-125 387980 Alia Al-Ghamdi M 517.5 15.3 -Cd-126 444268 Nowir Eqab M 505.7 14.0 -Cd-127 454772 Salma Habib F 518.5 14.3 -Cd-128 411062 Thuraya A M 27.9 0.7 -Cd-129 440813 Fawziah Salem F 438.8 13.2 -Cd-130 440173 Aala Mostafa Sarhar M 545.7 14.6 -Cd-131 363254 Saleh Musleh M 487.5 13.9 -Cd-132 450168 Khouloud Al Najar M 534.6 12.7 -Cd-133 441261 Muneera Ayed M 350.0 10.0 -Cd-134 348833 Huda Salih M 472.2 13.0 -Cd-135 441104 Huda Mohd M 550.1 14.5 -Cd-136 370268 Suuni Mabia F 552.7 14.8 -Cd-137 431537 Abeer Omer F 339.0 9.2 -Cd-138 455064 Noora Saeed F 518.5 16.7 -Cd-139 242815 Mariam Hamad F 349.1 9.8 -Cd-140 414017 Fatimah Salih M 401.3 11.4 -Cd-141 399983 Radwa Bishi F 457.9 13.4 -Cd-142 447718 Ghadha Mohd M 386.5 10.8 -Cd_143 428081 Abeer Omer F 418.1 10.6 -Cd-144 283024 Saidah Jabel F 334.4 9.3 -Cd-145 278631 Fatma Ahmed F 368.2 10.5 -Cd-146 455047 Fatma Harbi M 443.7 12.3 -Cd-147 427116 Tahani Mohd M 532.9 16.6 -Cd-148 283048 Aminah Abdullah F 583.9 14.3 -Cd-149 428759 Hoda Saeed F 563.6 15.9 -Cd-150 425385 Maryam Mafrah M 474.0 14.0 -



Appendix V: Datasheet for G6PD & DNA in G6PD Deficient Samples at JeddahSample

No.Nationality G6PD Conc. DNA Conc.

ul/ugVariants

u/gHb g/1012RBCD-1 Saudi 0.14 3.64 5.41 AuresD-2 Saudi 0.40 11.90 1.25 MediterraneanD-3 Saudi 0.78 27.30 1.46 MediterraneanD-4 Saudi 0.80 20.90 1.39 MediterraneanD-5 Saudi 0.85 28.10 2.43 MediterraneanD-6 Saudi 1.10 34.90 6.19 MediterraneanD-7 Saudi 1.15 34.30 1.49 AuresD-8 Saudi 1.20 36.30 1.59 MediterraneanD-9 Saudi 1.55 45.00 0.28 Mediterranean

D-10 Saudi 3.40 44.17 0.38 MediterraneanD-11 Saudi 3.95 122.00 0.36 MediterraneanD-12 Saudi 4.15 129.40 1.39 MediterraneanD-13 Saudi 4.16 101.40 0.46 MediterraneanD-14 Saudi 0.71 27.94 2.71 MediterraneanD-15 Saudi 2.30 68.80 1.79 AuresD-16 Saudi 4.20 113.90 2.63 MediterraneanD-17 Saudi 4.40 146.00 0.82 MediterraneanD-18 Saudi 4.00 102.90 137 MediterraneanD-19 Saudi 1.49 38.00 0.55 AuresD-20 Saudi 4.60 38.00 1.06 MediterraneanD-21 Saudi 0.24 29.00 2.44 MediterraneanD-22 Saudi 0.63 20.00 20.9 MediterraneanD-23 Saudi 0.34 7.60 1.27 MediterraneanD-24 Saudi 0.63 20.90 4.55 AuresD-25 Saudi 0.65 13.52 5.13 AuresD-26 Saudi 3.15 102.80 1.25 MediterraneanD-27 Saudi 3.36 102.80 1.46 MediterraneanD-28 Saudi 3.85 129.50 1.39 MediterraneanD-29 Saudi 3.98 130.00 2.43 MediterraneanD-30 Saudi 0.15 4.45 6.19 MediterraneanD-31 Saudi 0.58 19.95 1.49 MediterraneanD-32 Saudi 0.68 17.88 1.59 MediterraneanD-33 Saudi 2.28 73.70 0.28 MediterraneanD-34 Saudi 3.99 116.10 0.38 MediterraneanD-35 Saudi 4.16 149.20 0.36 MediterraneanD-36 Saudi 4.25 123.60 1.39 MediterraneanD-37 Saudi 4.39 139.00 0.46 MediterraneanD-38 Saudi 4.50 139.40 2.71 MediterraneanD-39 Saudi 4.59 96.10 1.79 MediterraneanD-40 Saudi 0.80 24.00 2.47 MediterraneanD-41 Saudi 1.11 30.39 0.82 MediterraneanD-42 Saudi 0.48 8.15 1.48 MediterraneanD-43 Saudi 2.40 70.00 0.55 Mediterranean

Contd………





ul/ugVariants

u/gHb g/1012RBCD-44 Saudi 1.20 45.00 1.68 MediterraneanD-45 Saudi 1.55 55.00 2.44 MediterraneanD-46 Saudi 3.40 50.00 20.9 MediterraneanD-47 Saudi 3.95 50.00 1.27 MediterraneanD-48 Saudi 4.15 155.00 4.55 MediterraneanD-49 Saudi 4.16 169.00 5.13 MediterraneanD-50 Saudi 0.71 46.00 1.49 AuresD-51 Saudi 2.30 40.00 1.59 MediterraneanD-52 Saudi 4.20 90.00 0.28 MediterraneanD-53 Saudi 4.40 58.00 0.38 MediterraneanD-54 Saudi 4.15 155.00 0.36 MediterraneanD-55 Saudi 4.16 169.00 1.39 MediterraneanD-56 Saudi 1.49 27.30 0.46 MediterraneanD-57 Saudi 0.78 20.90 2.71 MediterraneanD-58 Saudi 0.80 28.10 1.79 MediterraneanD-59 Saudi 0.85 34.90 2.26 MediterraneanD-60 Saudi 1.10 34.30 0.82 MediterraneanD-61 Saudi 1.15 36.30 1.03 MediterraneanD-62 Saudi 1.20 45.00 0.55 MediterraneanD-63 Saudi 1.55 44.17 1.67 AuresD-64 Saudi 3.40 122.00 2.44 MediterraneanD-65 Saudi 3.95 129.40 20.9 MediterraneanD-66 Saudi 4.15 155.00 1.27 MediterraneanD-67 Saudi 4.16 165.00 4.55 MediterraneanD-68 Saudi 0.71 19.50 5.13 MediterraneanD-69 Saudi 2.30 70.00 2.43 MediterraneanD-70 Saudi 4.20 155.00 6.19 MediterraneanD-71 Saudi 4.40 165.00 1.49 MediterraneanD-72 Saudi 0.15 4.45 1.59 MediterraneanD-73 Saudi 0.58 19.95 0.28 MediterraneanD-74 Saudi 0.68 17.88 0.38 MediterraneanD-75 Saudi 2.28 73.70 0.36 MediterraneanD-76 Saudi 3.99 116.10 1.39 MediterraneanD-77 Saudi 0.68 17.88 0.46 AuresD-78 Saudi 4.25 123.60 2.71 MediterraneanD-79 Saudi 4.39 139.00 1.79 MediterraneanD-80 Saudi 4.50 139.40 2.06 MediterraneanD-81 Saudi 4.72 96.10 0.82 MediterraneanD-82 Saudi 0.80 24.00 1.42 MediterraneanD-83 Saudi 1.11 30.39 0.55 MediterraneanD-84 Saudi 0.85 24.20 1.63 MediterraneanD-85 Saudi 1.60 55.80 2.44 MediterraneanD-86 Saudi 3.00 138.50 20.9 ChattamD-87 Saudi 1.80 59.40 1.27 Mediterranean

Contd. ….



Appendix V: Datasheet for G6PD & DNA Concentration in G6PDDeficient Samples at Jeddah

SampleNo.

Nationality G6PD Conc. DNA Conc.ul/ug

Variantsu/gHb g/1012RBC

D-88 Saudi 0.60 19.30 4.55 AuresD-89 Saudi 1.50 54.20 5.13 MediterraneanD-90 Saudi 3.95 130.00 1.25 MediterraneanD-91 Saudi 4.15 129.00 1.46 MediterraneanD-92 Saudi 4.16 136.00 1.39 MediterraneanD-93 Saudi 0.71 58.00 2.43 MediterraneanD-94 Saudi 2.30 120.00 6.19 MediterraneanD-95 Saudi 4.20 126.00 1.49 MediterraneanD-96 Saudi 4.40 146.00 1.59 MediterraneanD-97 Saudi 3.08 102.90 0.28 MediterraneanD-98 Saudi 0.40 11.90 0.38 MediterraneanD-99 Saudi 0.78 27.30 0.36 Mediterranean

D-100 Saudi 0.80 20.90 1.39 MediterraneanD-101 Saudi 0.85 28.10 0.46 MediterraneanD-102 Saudi 1.10 34.90 2.71 MediterraneanD-103 Saudi 1.15 34.30 1.79 MediterraneanD-104 Saudi 1.20 36.30 2.33 MediterraneanD-105 Saudi 1.55 45.00 0.82 AuresD-106 Saudi 3.40 44.17 1.67 MediterraneanD-107 Saudi 3.95 122.00 0.55 MediterraneanD-108 Saudi 4.15 129.40 1.63 MediterraneanD-109 Saudi 4.16 101.40 2.44 MediterraneanD-110 Saudi 0.71 27.94 20.9 MediterraneanD-111 Saudi 2.30 68.80 1.27 MediterraneanD-112 Saudi 4.20 113.90 4.55 MediterraneanD-113 Saudi 4.40 146.00 5.13 MediterraneanD-114 Saudi 4.00 102.90 1.49 MediterraneanD-115 Saudi 1.49 136.50 1.59 MediterraneanD-116 Saudi 4.97 38.00 0.28 MediterraneanD-117 Saudi 0.24 29.00 0.38 MediterraneanD-118 Saudi 0.63 20.00 0.36 MediterraneanD-119 Saudi 0.80 24.00 1.39 MediterraneanD-120 Saudi 0.85 36.00 0.46 MediterraneanD-121 Saudi 1.10 44.00 2.71 MediterraneanD-122 Saudi 1.15 44.00 1.79 AuresD-123 Saudi 1.20 45.00 2.24 MediterraneanD-124 Saudi 1.55 55.00 0.82 MediterraneanD-125 Saudi 3.40 50.00 1.43 MediterraneanD-126 Saudi 3.95 50.00 0.58 MediterraneanD-127 Saudi 4.15 155.00 1.36 MediterraneanD-128 Saudi 4.16 169.00 2.44 Mediterranean

Contd…





ul/ugVariants

u/gHb g/1012RBCD-129 Saudi 0.71 46.00 20.9 AuresD-130 Saudi 0.40 11.90 1.27 MediterraneanD-131 Saudi 0.78 27.30 4.55 MediterraneanD-132 Saudi 0.80 20.90 5.13 MediterraneanD-133 Saudi 0.85 28.10 2.44 MediterraneanD-134 Saudi 1.10 34.90 20.91 AuresD-135 Saudi 1.15 34.30 1.27 AuresD-136 Saudi 1.20 36.30 4.55 MediterraneanD-137 Saudi 1.55 45.00 5.13 MediterraneanD-138 Saudi 3.40 44.17 1.49 MediterraneanD-139 Saudi 0.57 19.95 1.59 MediterraneanD-140 Saudi 4.56 96.10 0.28 MediterraneanD-141 Saudi 0.65 13.52 0.38 MediterraneanD-142 Saudi 0.14 4.45 0.36 MediterraneanD-143 Saudi 1.44 38.00 1.39 AuresD-144 Saudi 1.11 30.39 0.46 MediterraneanD-145 Saudi 8.15 0.48 2.71 MediterraneanD-146 Saudi 0.48 45.30 1.79 MediterraneanD-147 Saudi 2.19 39.50 2.14 MediterraneanD-148 Saudi 1.95 42.70 1.27 AuresD-149 Saudi 0.24 29.00 4.55 MediterraneanD-150 Saudi 0.63 20.00 5.13 MediterraneanD-151 Saudi 0.65 13.52 0.83 MediterraneanD-152 Saudi 0.14 4.45 0.36 MediterraneanD-153 Saudi 1.44 38.00 1.19 AuresD-154 Saudi 2.07 30.89 0.76 MediterraneanD-155 Saudi 8.15 0.48 2.71 MediterraneanD-156 Saudi 0.48 35.60 1.79 MediterraneanD-147 Saudi 2.19 49.30 2.17 MediterraneanD-158 Saudi 1.97 47.20 1.06 AuresD-149 Saudi 0.24 39.00 3.62 MediterraneanD-160 Saudi 0.63 23.50 4.13 MediterraneanD-161 Saudi 4.50 139.40 2.71 MediterraneanD-162 Saudi 2.59 96.10 1.79 MediterraneanD-173 Saudi 0.80 24.00 2.02 MediterraneanD-124 Saudi 0.85 36.00 0.46 MediterraneanD-165 Saudi 4.25 123.60 1.39 MediterraneanD-166 Saudi 4.39 139.00 0.46 MediterraneanD-167 Saudi 3.72 129.40 2.71 MediterraneanD-168 Saudi 4.53 96.10 1.79 MediterraneanD-169 Saudi 0.80 22.40 1.27 MediterraneanD-170 Saudi 4.16 169.00 2.44 Mediterranean



Appendix VI: Datasheet for Various Polymorphisms in G6PD DeficientSamples at Jeddah

25 Male + 4 Female= 33 chromosomes

Both (Dual) = C1311 C or T & IVS-XI=93 T or C, - = None

SampleNo.

C1311C or T

IVS-XI=93T or C

C1311 C or T &IVS-XI=93 T or C (Dual)

Variant

1 T C Both -2 T C Both Mediterranean3 T C Both Mediterranean4 C T Nil -5 T C Both Mediterranean6 T C Both Aures7 C C IVS-XI=93 T or C -8 C C IVS-XI=93 T or C -9 C T Nil -

10 C C IVS-XI=93 T or C -11 T C Both Mediterranean12 C T Both Aures13 C C Nil -14 T C Both Aures15 T C Both Mediterranean16 T C Both Aures18 C T Nil -19 C T Nil -20 C C IVS-XI=93 T or C -21 T C Both Mediterranean22 C T Nil -23 C T Nil -24 C C IVS-XI=93 T or C -25 C T IVS-XI=93 T or C -26 T C Both Mediterranean27 C T Nil -28 C C IVS-XI=93 T or C -29 C T Nil -30 C T Nil -31 C T Nil -32 T C Both Mediterranean33 T C Both Mediterranean

Molecular Characterization of Erythrocyte Glucose- 6 -Phosphate Dehydrogenase Deficiency in Jeddah, Saudi Arabia

Appendix VII: Review Article

Glucose-6-phosphate dehydrogenase (G6PD) Deficiency

D. D. Farhud, L. Yazdanpanah

Iranian J Publ Health, Vol. 37, No. 4, 2008, pp. 1-18

Table 4: Drugs and substances should be avoided by G6PD deficient individuals

Drugs and substances (group) Examples

Antimalarial drugs Primaquin

Sulphonamides Sulphacetamid, Sulphametoxazole Sulphanilamid,

Sulphapyridin

Sulphones Thiazolesulfone, Dapsone

Other sulphur containing drugs Glibenclamide

Nitrofurans Nitrofurantoin (Furadantin)

Other drugs Toluidin blue, Trinitrotoluene (TNT), Urate oxidase,

Phenylhydrazine, Furazolidone (Furoxone), Methylene

Blue, Nalidixic acid, Niridazole, Phenazopyridine,

Isobutyl Nitrite, Acetanilide, Aspirin

Cosmetic substance Henna

Other substances Naphthalene, Mothballs, High-dose of vitamin K or

ascorbic acid



Table 5: Drugs and substances (with their molecular formulation) should be avoided by G6PDdeficient individuals according to the G6PD Deficiency Association

Name Risk Levels Populationat Risk

AcetanilideAcetylphenylhydrazineAldesulfonesodium(sulfoxone)Aminophenazone aminopyrine)(Antazoline (antistine)ArsineAscorbicAcidBeta-Naphthol (2-Naphthol)ChloramphenicolChloroquineCiprofloxacinColchicineDapsone (diaphenylsulfone)DimercaprolDiphenhydramine (difenilhydramine)Dopamine (L-dopa)DoxorubicinFurazolidoneGlibenclamideGlucosulfone (glucosulfonesodium)Isobutyl NitriteIsoniazidMenadiolSodiumSulfate(Vitamin k4Menadione (menaphtone)MenadionesodiumBisulfite (VitaminK3 )Mepacrine (Quinacrine)Mesalazine-5-AminosalicylicAcidMethyltioninium Chloride (methyleneblue)Nalidixic AcidNaphtalene, Pure (naphtalin)NiridazoleNitrofural (nitrofurazone)NitrofurantoinNorfloxacinO-Acetylsalicylic Acid (acetylsalicylicacid)O-Acetylsalicylic Acid (acetylsalicylicacid)Oxidase,Urate (urateoxidase)

HighHighHighLowLowHighLowHighHighHighHighLowHighHighLowLowHighHighHighHighHighLowHighHighHighHighHighHighHighHighHighHighHighLowHighHigh

HighHigh

Medit.,AsianAllAllAllAllAllAllAllMedit.,AsianMedit., AsianMedit., AsianAllAllAllAllAllMedit.,AsianAllMedit.,AsianAllMedit.,AsianAllAllAllAllMedit.,AsianMedit.,AsianAllMedit.,AsianAllAllAllAllAllMedit.,Asian

Medit.,AsianMedit.,Asian

10



Table5: continued

Name Risk Levels PopulationatRisk

PamaquinePara-AminobenzoicAcidParacetamol (acetaminophen)PentaquinePhenacetin (acetophenetidin)Phenazone (antipyrine)PhenazopyridinePhenylbutazonePhenytoinPhynylhydrazinePhytomenadione (VitaminK1)PrimaquineProbenecidProcainamideProguanil (chlorguanidine)PyrimethamineQuinidineQuinineStibophenStreptomycinSulfacetamideSulfacytine SulfadiazineSulfadimidineSulfafurazole (sulfafurazone,sulfisoxazole)SulfaguanidineSulfamerazineSulfamethoxazoleSulfanilamideSulfapyridineSulfasalazine,SalazosulfapyridineThiazosulfone (thiazolesulfone)TiaprofenicAcidToluidine blueTrihexyphynidyl (benzhexol)TrimethoprimTrinitrotoluene (2,4,6-Trinitrotoluene)Tripelennamine

HighLowLowHighHighLowHighLowLowHighLowHighHighLowLowLowLowLowHighLowHighLowLowHighHighLowLowHighHighHighHighHighLowHighLowLowHighLow

AllAllAllAll

Medit.,AsianAll

Medit.,AsianAllAllAllAllAllAllAllAllAllAllAllAllAllAllAllAllAll

Medit.,AsianAllAllAllAllAllAll

Medit.,AsianAllAllAllAll

Medit.,AsianMedit.,Asian

Food substances and herbs that should be avoided by G6PD deficient peopleFavaBeans. Some prefer also to avoid redwine, all legumes, blueberries (also with yogurt) ,s oya products, tonic water. Chinese Herbs to Avoid: Cattle Gallstone Bezoar(Bos Taurus Domesticus), Honeysuckle (LoniceraJaponica), Chimonanthus Flower(Chimonanthus Praecox), Huang Lian, 100% Pearl Powder 1 1

8. appendicesshodhganga.inflibnet.ac.in/bitstream/10603/71531/15/15_appendix.pdfmolecular...

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