PATENT ABSTRACTS

8600339

A PROCESS AND AN APPARATUS FOR THE RECOVERY OF A

POLYPEPTIDE FROM A FERMENTATION BROTH

John PEDERSEN, Kim Ry HEJNA@ES, Tjo@/mevej 7, DK-2970 Ho@/rsholm, Den- mark assigned to NORDISK GENTOFTE A/S

In a process for recovering a polypeptide, such as an insulin-like material or hGH, from a fer- mentation broth, the fermentation broth is pas- sed through a bed of a chromatographic material containing hydrophobic groups, such as phenyl sepharose, and then the adsorbed insulin material is eluted with an aqueous medium, which, if desired, may contain a water miscible organic solvent. When the chromatographic separation is carried out under such conditions, the polypeptide is isolated in a high yield in a single step from the fermentation broth and the other components contained in the broth. Fur- ther, a high concentration factor is obtained. An apparatus for carrying out the process consists of a fermentation container (1) and at least one chromatographic column (8, 9) connected with the container, as well as means (2, 7, 10) for cir- culating the fermentation broth from the fer- mentation container (I) through the chromatographic column or columns (8, 9).

86OO34O

PROCESSES AND MATERIALS FOR CARRYING OUT

MICROCHEMICAL AND MICROBIOLOGICAL TESTS

Thomas Stewart BEGGS, Thomas John SANDS, 70 Somerford Road, Wellingborough, Northants NN8 3EZ, United Kingdom assigned to UNILEVER NV

A microbiological culture test process for detec- ting the presence of tryptophan-deaminase- positive microorganisms, which comprises (a) culturing a microorganism to be tested in the presence of L-tryptophan in an effective amount and for a period sufficient to allow production of indolepyruvic acid by the organism if it is of tryptophan-deaminase-positive character, (b) exposing the culture to a chromogenic amount of metal ions which are chromogenic with in- dolepyruvate, and to a fluorophor, and (c) asses- sing the fluorescence of the culture after steps (a)

323

and (b), thereby to show a reduction in fluores- cence in the presence of a tryptophan- deaminase-positive microorganism.

8600341

PROCESSES AND MATERIALS FOR CARRYING OUT

MICROCHEMICAL AND MICROBIOLOGICAL TESTS

Thomas John SANDS, 52B Somerford Road. Wellingborough, Northants NN8 3EZ, United Kingdom assigned to UNILEVER NV

A microbiological cultuce test process for detec- ting the presence of nitrate-reducing micro- organisms in an inoculum sample, which comprises (a) culturing a microorganism to be tested in the presence of adequately nitrite-free nitrate, for a period suflicient to allow reduction of nitrate by any microorganism (if present) that has the capacity to reduce nitrate to nitrite, (e.g. 1-6 hours); (b) exposing the culture medium after culture to (diazotising) acid conditions in the presence of an amino-containing fluorophor thereby to cause diazotisation of the fluorophor to the extent of any nitrite present in the medium to form a diazonium derivative (or further reac- tion product thereof) which is substantially less fluorescent (or fluoresces at a substantially dif- ferent wavelength) than said amino-containing fluorophor, and (c) assessing the fluorescence of the culture medium as treated by step (b), thereby to show a reduction in fluorescence in the presence of a nitrate-reducing micro- organism.

8600342

PROBE AND METHOD FOR THE DIAGNOSTIC OF THE SEX OF A

HUMAN EMBRYO

Gilles VERGNAUD, Liliana KAPLAN, Jean WEISSENBACH, Pierre TIOLLAIS, Georges GUELLAEN, 114, avenue Victor Hugo, F- 92170 Vanves, France assigned to INSTITUT PASTEUR; CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE; INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE M

Probe and method for the detection in vitro of the sex of a human embryo by hybridation of the probe with nucleic acids of cells from said em- bryo. The method is implemented with a probe

324 PATENT ABSTRACTS

containing a fragment of DNA hybridable with the fragment EcoRI of 2.8 kb obtained from the euchromatic region of the large arm of the chromosome Y and deposited on the 29/06/1984 at the Collection Nationale des Cultures de Micro-organismes under the No 1/312, said hybridation being performed or maintained in the presence of a solution 0.1 x SSC, at 65- 68(o)C, more particularly 68(o)C.

8600343

M E T H O D O F D E T E R M I N I N G C Y S T I C F I B R O S I S C I L I O S T A T I C

F A C T O R

Thomas J KELLEHER, Paul T NIX assigned to COOPERBIOMEDICAL INC,

A method for detecting the presence of cystic fibrosis ciliostatic factor in mammalian body fluid comprises contacting an enzyme whose ac- tivity is inhibited by the factor with a substrate for the enzyme in the presence of the body fluid, the substrate being substantially resistant to reactions catalyzed by enzymes in the body fluid,

whereby the substrate is converted by the en- zyme at a measurable rate, and comparing the rate of substrate conversion with the rate of sub- strate conversion by the enzyme in the absence of cystic fibrosis ciliostatic factor.

8600413

D I A G N O S T I C A S S A Y F O R I N H I B I T O R O R T I S S U E - T Y P E

A N D U R O K I N A S E - T Y P E P L A S M I N O G E N A C T I V A T O R S

David J LOSKUTOFF, Raymond R SCHLEEF assigned to SCRIPPS CLINIC AND RESEARCH FOUNDATION

A biochemical reagent system and methods for preparing and using same, as well as diagnostics utilizing the reagent system. The biochemical reagent system comprises a receptor raised in an animal host to plasminogen activator inhibitor, and indicating means. The receptor binds to a specific plasminogen activator inhibitor that it- self binds to tissue-type or urokinase-type plas- minogen activators.