04/19/23 Slide 1

Oral Presentation for Chem 201:

Title of Lab Goes Here

U.R. Name

Lab Partner: T.H. Guy

Presentation date: 04/19/23

04/19/23 Slide 2

Introduction

The INTRODUCTION introduces the talk Include in your Introduction

The problem you addressed Your experimental approach to the problem The significance of your work

Other ground rules: Bullet points are best

Bullet points should be just that Say full sentences; don't write them

Don't use too many colors Don't put too much material on one "slide"

Use more slides than less slides Make sure that everything you present is legible. Use 14-point type or bigger

Leave white space as a border around every slide!

04/19/23 Slide 3

Details of the Experiment

Because you can only put one topic/thought per slide, you will need several slides to fill in critical details.

The slides that come after the Introduction will be the equivalent of the Experimental section Provide any theory required that is critical to the calculations and

results you will obtain Provide an overview of the instrumentation used and any details

about instrumentation that are critical to the data you obtained Describe the experiments you have performed Present the critical equations for your calculations of data Present any other critical details

04/19/23 Slide 4

Details – Cont.

State logically (in stepwise fashion) the experimental details and what you did

Give big strokes—not tedious details. Examples: Prepared solutions of dimuglioglugtane (DMGG) at concentrations of

0.01, 0.1, and 1 M in CDCl3. Acquired 1H-NMR spectra

If the number of spectra or other acquisition parameters are important, include as sub-points.

Tell how you transformed the raw data into meaningful results Make sure you keep everyone focused on the purpose of the

experiment Discuss the importance of your findings after you present your

results.

04/19/23 Slide 5

Other Points

Cite sources of information in your presentation. Including: Articles used in your "overview" etc. All figures that you present that are not your own Sources of information

Citations are best placed at the bottom of the slide

Pictures are always better than words Present the pictures; say the words Data tables:

Avoid where possible. Use bar graphs, etc. instead of data tables Keep the tables simple and small

Here is a good place to put any citations you need on any page.

04/19/23 Slide 6

Example of Introduction Using Both Graphical Info and Bullet Points:

Sulfur Amino Acids (SAA)

Methionine An essential amino acid

Cysteine Nonessential amino acid Synthesis dependent methionine

Homocysteine Produced from methionine metabolism Contributes S for cysteine synthesis

Elevated homocysteine levels correlate with cardiovascular disease risk

Methionine (Met)

NH2

O

HOS

CH3

Cysteine (Cys)

NH2

O

HO SH

Homocysteine(Hcy)

NH2

O

HOSH

04/19/23 Slide 7

Example of an Experimental Procedure in Graphical Terms:

Method to Reduce and ModifyProtein-bound Homocysteine for Measurement by GCMS

From MacCoss et al., Anal. Chem. 1999

NH2

OH

O

SS

N

NSH

O

O

H3C

H3C

SH

NH2

OH

O

SH N

N S

O

O

H3C

H3C

S

N

NH2

OH

O

S

NNH

OtBDMS

O

S

tBDMS

Homocysteine Bound to Protein via a Disulfide Bridge

+

MTBSTFA

S-4-ethyl pyridine bis-tBDMS homocysteine

1

2

3

Homocysteine

DMH

N

4-vinylpyridine

04/19/23 Slide 8

Graphical Example of Results:

EI & NCI Mass Spectra of Leucine as the HFBPr Derivative

60 80 100 120 140 160 180 200 220 240 260 280 300 3200

1000

2000

3000

4000

5000

6000

7000

Abundance

55

69

114 131169

214

240

253

266

282

313

m/z

[M-COOPr]+

[M-C4H8]+?

CF3+

HFB-NH2+ ?

EI

160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 4600

5000

10000

15000

20000

25000

Abundance

160

178

198 243281

311

349

368 452

m/z

[M-H]-

[M-HF]-

?NCI

HFB = heptafluorobutyrylPr = n-propyl ester

CHNO

O

C3H7

C3F7O

[M-COOPr]+

MW = 369

04/19/23 Slide 9

Presenting Results & Discussion

Don't give ALL the data and ALL the results you have obtained Determine what you believe are the key results, and present

them Organize your talk around the Discussion

Figure out what results are required to support the points you want to discuss. Those are the results that you need to present.

Some data may need to be presented just to confirm that you correctly collected the results that you think you collected Examples: Data that demonstrate the instrumentation being used is working Data that demonstrate the experimental conditions are being met

04/19/23 Slide 10

Graphical Example of an Almost Too Busy Table: Amino Acid Composition of Select Protein Sources

Amino AcidHen Egg Muscle Liver

mole %

Alanine Ala 9.2 9.5 9.9

Arginine Arg 4.8 5.0 4.3

Aspartate + Asparagine Asx 6.6 7.9 7.9

Cysteine Cys 2.4 1.5 1.8

Glutamate + Glutamine Glx 10.2 13.0 10.5

Glycine Gly 5.9 8.7 8.0

Histidine His 2.0 2.4 2.2

Isoleucine Ile 6.4 4.7 5.0

Leucine Leu 8.4 8.0 9.1

Lysine Lys 5.5 7.6 6.7

Amino AcidHen Egg Muscle Liver

mole %

Methionine Met 2.6 2.2 2.3

Phenylalanine Phe 3.3 3.6 4.1

Proline Pro 4.8 5.7 5.7

Serine Ser 10.6 6.3 6.7

Threonine Thr 5.3 0.5 5.2

Tryptophan Trp 1.0 0.7 1.0

Tyrosine Tyr 3.0 2.2 2.6

Valine Val 8.0 6.1 6.8

04/19/23 Slide 11

Graphical Example of the Table Data as an Almost Too Busy Bargraph: Amino Acid Composition of Select Protein Sources

0%

2%

4%

6%

8%

10%

12%

14%

Am

ino

Ac

id C

on

ten

t(m

ole

fra

cti

on

)

Hen EggMuscleLiver

highin

egg

eachare

double

lowin

egg

not very abundant

04/19/23 Slide 12

Example of an Annotated, Scanned Figure: ESI-MS Spectrum of Horse Apomyoglobin

ApomyoglobinMW=16,951.2

+18

+20

+16

+14

+12

+10+22

m/z

04/19/23 Slide 13

Conclusions

You must have one or more Conclusions slides

The Conclusions Reiterate the key points of what you have accomplished State (for the last time) what is important about what you have done

04/19/23 Slide 14

Additional Constraints

At the time of your oral presentation, you need to:

1. Distribute a 1-page abstract of your talk to the class, TA's and instructor

2. Distribute copies of the slides that you will use to your TA's & instructor Printed no more than 2 per page

3. Give an annotated appendix of all figures, tables, data, etc. to your TA This appendix is the same as you would prepare for a regular

written laboratory.

04/19/23 Slide 15

Final Points

Plan on speaking for 30 (25-35) min with 10 min at the end for questions

Practice your talk before you actually give it! Be on time and be ready to go

Technical problems will cost you valuable speaking time

You can use for your presentation Slides displayed from a computer projection device

Angell B203 has a PC w/ USB port (not a Mac) Overheads Blackboard (as supplemental)

You get up to 20 points (your presentation is worth 50 points) for participating in asking questions and discussing the presentations

If you not ask questions or contribute to the discussion of the talks, you don’t get any points

Generally one question per talk for 4-5 talks will get you the 20 points