a b c 3500x8900x 5600x14 000x 1500x5000x supplementary figure 1 tio 2 carbon black swcnt
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Supplementary Figure 1
TiO2
Carbon black
SWCNT
Supplementary Figure 2
DMEM
P-Iκ
BαDA
PIM
erge
SWCNT
WT p53 KO WT p53 KO
P-2κBα immunostaining of WT and p53 KO macrophages
Immunostaining quantification
Pe
rce
nta
ge
of
po
sitiv
ely
st
ain
ed
ce
lls (
%)
2µg/cm2
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B
Supplementary figure legends
Supp Fig 1. Transmission electron microscopy images of RAW 264.7 cells incubated with 10 µg/ml (2 µg/cm2) of manufactured nanomaterials (MNMs) for 6 hr. (A) RAW 264.7 cell with TiO2 A10 MNMs within a vesicle (B) CB P60 MNMs within the cytosolic compartment of a RAW cell. (C) RAW macrophages with SWCNT at the membrane surface.
Supp Fig 2. Consequence of p53 activation: inflammatory response in primary wild-type (WT) and p53 knock-out (KO) macrophages. (A, B) The cells were exposed to 2 µg/cm2 of TiO2 (A10), CB (FW2) and SWCNT MNMs for 30 min. The data show the percentages of phosphorylated IκBα (P- IκBα) positively stained macrophages (mean ± SEM) (n=3). * significant difference from WT control (p < 0.05). # significant difference between WT and p53 KO macrophages (p < 0.05).