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  • Dr. Arthur Chiou Institute of Biophotonics

    School of Biomedical Science & Engineering Biophotonics Interdisciplinary Research Center

    National Yang-Ming University Taipei, Taiwan

    aechiou@ym.edu.tw

    A Brief Introduction to Biophotonics with Selected Illustrative Examples

    A Brief Introduction to A Brief Introduction to BiophotonicsBiophotonics with Selected Illustrative Exampleswith Selected Illustrative Examples

  • geology.com

  • 1994~Present National Yang-Ming University

    1975~19941975~1994

    National YangNational Yang--Ming Medical CollegeMing Medical College

    NYMUNYMU-- HistoryHistory

    A research university that focuses on life science and medicine.

  • NYMUNYMUNYMU

    Taipei City Hospital System Veterans General Hospital-Taipei Veterans General Hospital-Taichung Veterans General Hospital-Kaohsiung

    Leading medical centers in Taiwan More than 10,000 beds

    NYMU Teaching Hospital SystemNYMU Teaching Hospital System

    4 teaching hospitals

    39 affiliated hospitals

  • National YangNational Yang--Ming UniversityMing University Taipei, TaiwanTaipei, Taiwan

    1975 National Yang-Ming Medical College 1994 National Yang-Ming University

    School of Medicine

    School of Bio-Medical Science

    & Engineering

    School of Life Science

    School of Nursing

    School of Dentistry

    General Education Center

  • Faculty (2007): 297

    NYMUNYMU-- Faculty Faculty BodyBody

    Lecturer

    Professor

    Associate ProfessorAssistant

    Professor

    102 (34%)

    114 (39%)39 (13%) 42 (14%)

  • Students (2007): 3,815

    Undergraduate ~850 med students

    Graduate PhD

    Master

    NYMUNYMU-- Student CompositionStudent Composition

    632 (17%)

    1,210 (32%) 1,973 (52%)

  • School of BioSchool of Bio--Medical ScienceMedical Science & Engineering& Engineering

    Baccalaureate Programs • Faculty of Medical Technology • Faculty of Physical Therapy • Faculty of Radiological Technology

    Master & PhD Degree Programs • Institute of Biomedical Engineering • Institute of Biotechnology in Medicine • Institute of Radiological Science • Institute of Biophotonics • Institute of Physical Therapy & Rehabilitation Science

  • Institute of Institute of BiophotonicsBiophotonics School of Biomedical Science & EngineeringSchool of Biomedical Science & Engineering

    7 Full-time Faculty Members 16 Adjunct Faculty Members

    2 (to 3) Year Master Degree Program (since 2001) 40 MS Students (currently)

    25 with Phys. and Eng. Background 15 with Bio. Background

    30 New MS Students (08/2008)

    3 (to 5) Year PhD Program (since 2006) 11 PhD Students (currently)

    7 with Phys. and Eng. Background 4 with Bio. Background

    10 New PhD Students (08/2008)

  • NYMU-VGH-TCH AS-NHRI-NDL-NSRL

    Biophotonics & Nanobiophotonics Team

    Biophotonics Inter-disciplinary Research Center (BIRC)

  • NYMUNYMU-- National Core FacilityNational Core Facility

    • National Genomic Medicine Projects

    • National Nano Science Technology Program

    • National Genome Sequencing Center • National Microarray and Gene

    Expression Center • National Micro-Pet and Gene Probe

    Center • National NanoBio Technology Core

  • Complex Sciences New Paradigms

    New Technologies

    TopicsTopics--orientated Researchorientated Research

    Genome Research

    Brain Research

    Immunology Research

    Biophotonics Research

    Received an awarded from Ministry of Education of Taiwan- “Aim for Excellence, 5 years 50 billion NT$” Program, 2006-2010

  • Enable platform

    technology

    Microscopy

    &

    Spectroscopy

    In vivo imaging

    Magnetic resonance Imaging (MRI)

    In vivo optical imaging (GFP/RFP, bioluminscence)

    micro-SPECT/CT

    micro-PET

    Animal sonogram

    Fiber-optical dual- beam trap

  • BioPhotonics

    Biosensing

    Biomanipulation

    Microscopy/spectroscopy

    Raman/SERS Nanowire

    SPR SMD

    Optical capturing and stretching Tissue engineering

    Nanofabrication

    Molecule Cell Tissue Animal

    FLIM/FRET Photoactivation

  • Photonics Life Science Medical Science

    Biophotonics

    Photonics Science & Technology for Biomedical Applications Biological Materials for Photonics Applications

    The Interaction of Photons with Biological Materials

  • Biophotonics

    Medical Applications Fundamental Physics & Chemistry of Life at the Molecular Level

    Diagnostics Therapeutics

    Imaging Sensing

    Photodynamic Therapy (PDT) Light-Guided Gene Therapy Light-Activated Therapy Laser Tissue Welding Laser Surgery Nano-medicine

    Imaging & Sensing at the Molecular & Cellular Levels

    DNA Mechanics Cellular Mechanics Plasma Membrane Dynamics

  • Magnetic resonance Imaging (MRI)

    In vivo optical imaging (GFP/RFP, bioluminscence)

    nano-SPECT/CT

    micro-PET

    Animal sonogram

    Genes Dev 17: 545-580, 2003

    Multimodality In Vivo Imaging

    黃正仲黃正仲、、王信二王信二 、、劉仁賢劉仁賢 、、

    陳志成陳志成、、李俊信李俊信 、、林慶波林慶波 、、

    陳潤秋陳潤秋

  • COMPARING DIFFERENT SMALL ANIMAL COMPARING DIFFERENT SMALL ANIMAL MOLECULAR IMAGING TECHNIQUESMOLECULAR IMAGING TECHNIQUES

    YesYesYesYesYesYesYesYesYesYesTranslationalTranslational

    ExcellentExcellentGoodGoodNoNoPoorPoorNoNo Molecular Molecular targeting targeting

    sensitivitysensitivity

    ExcellentExcellentExcellentExcellentNoNoGoodGoodNoNoMolecular Molecular TargetTarget

    ~1 mm~1 mm~1 mm~1 mmSubSub-- millimetermillimeter

    SubSub-- millimetermillimeter

    SubSub-- millimetermillimeterResolutionResolution

    YesYesYesYesNoNoYesYesYesYesFunctionalFunctional

    NoNoNoNoYesYesYesYesYesYesAnatomicalAnatomical

    PETPETSPECTSPECTXX--ray CTray CTMRIMRIUSUS

    YesYes

    PoorPoor

    ExcellentExcellent

    SubSub-- micronmicron

    YesYes

    NoNo

    OPTICALOPTICAL

  • Monitoring the Monitoring the Tumor Tumor Growth/ProgressionGrowth/Progression with BLIwith BLI

    Week 3 Week 4 Week 5 Week 6 Week 7 Week 8 Week 9 Week 10

    Week 11 Week 12 Week 13

    Mean tumor volume (mm3)

    0 250 500 750 1000 1250 1500 1750 2000 2250

    M ea

    n bi

    ol um

    in es

    ce nc

    e (p

    h/ s)

    1e+7

    1e+8

    1e+9

    A

    B

  • Week 4 week 6 week 8 week 9 week 10 week 11 week 12 week 13

    Week 14 week 15 week 16 week 17

    weeks after tumor inoculation 2 4 6 8 10 12 14 16 18

    Ph ot

    on fl

    ux (p

    h/ s)

    1e+4

    1e+5

    1e+6

    1e+7

    1e+8

    1e+9

    thorax

    BLI for tumor progression and BLI for tumor progression and metastasismetastasis

  • • Interference fringes are observed only when optical path lengths are matched within coherence length of the source

    • Axial resolution: coherence length of the source: 1-15 µm

    Three reflecting surfaces

    SLD

    Reference mirror

    Photodetector

    Beam splitter

    Optical Coherence TomographyOptical Coherence Tomography

  • • Cancer diagnosis (skin, GI, respiratory, and urogenital tracts, etc.) • Burn depth determination and tissue viability monitoring • Vasoactive drug screen • Surgical guidance (brain, etc.) • Developmental biology • Ocular diseases

    GI tract

    Brain

    Oral cancers

    Eye

    larynx

    Cochlear

    Tracheal

    skin

    Applications

    Drug screen

    Burn depth

    Drug screen

  • Biophotonics

    Medical Applications Fundamental Physics & Chemistry of Life at the Molecular Level

    Diagnostics Therapeutics

    Imaging Sensing

    Photodynamic Therapy (PDT) Light-Guided Gene Therapy Light-Activated Therapy Laser Tissue Welding Laser Surgery Nano-medicine

    Imaging & Sensing at the Molecular & Cellular Levels

    DNA Mechanics Cellular Mechanics Plasma Membrane Dynamics

  • Ti:Sapphire Oscillator

    PMT

    Pinhole

    Fiber Coupler

    Filter Dichroic Mirror

    Fiber

    λ= 830 nm 80 fsPulse Duration

    Sp ec

    tr om

    et er Photon Counter

    Pulse Stretcher

    TwoTwo--photon optical fiber fluorescence probe photon optical fiber fluorescence probe for for in vivoin vivo biosensingbiosensing

    M.T. Myaing, J.Y. Ye, T.B. Norris, T. Thomas, J. Baker, Jr., W.J. Wadsworth, G. Bouwmans, J.C. Knight, and P.St.J. Russell, Enhanced Two-photon Biosensing With Double-clad Photonic Crystal Fibers," Optics Letters (in press)

  • Implantable Co-Axial Microlight Guide

  • * *

    On-going and potential projects:

    1. Monitor chemo/radiation therapy response of colorectal cancer

    2. Monitor low dose PDT res

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