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A new vine disease in Trentino: interdisciplinary approach to the study of issue. Territorial monitoring. CENTRO TRASFERIMENTO TECNOLOGICO Franca Ghidoni, Roberto Lucin, Maurizio Bottura, Valeria Gualandri FEM – San Michele all’Adige – Trento (Italy)

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A new vine disease in Trentino: interdisciplinary approach to the study of

issue. Territorial monitoring.

CENTRO TRASFERIMENTO TECNOLOGICO

Franca Ghidoni, Roberto Lucin, Maurizio Bottura, Va leria GualandriFEM – San Michele all’Adige – Trento (Italy)

A new symptom appear in Trentino vineyards since 2004

CENTRO TRASFERIMENTO TECNOLOGICO

Afterwards…

Reported also in:� Friuli Venezia Giulia�Veneto�Emilia Romagna

CENTRO TRASFERIMENTO TECNOLOGICO

…and in Trentino???...

CENTRO TRASFERIMENTO TECNOLOGICO

Only on few varieties:PINOT GRIS (and other PINOTS)

TRAMINER(FRIULANO OR TOKAY in Friuli;

GLERA in Veneto)

Between 2004 and 2009 symptoms noted only in Mezzocorona: “Zablani”

Since 2009 also in neighbour towns

…and in the rest of the world?

Detected in Korea, in Slovakia and Czech Republic

(Miroslav Glasa, personal communication)

CENTRO TRASFERIMENTO TECNOLOGICO

Pinot Grigio; 25%

Traminer; 3,60%

Pinot Bianco; 0,70%

Pinot Nero ; 2,70%

Enantio; 0,40%

Other varieties; 39,20%

Chardonnay; 28,40%

Economic importance

CENTRO TRASFERIMENTO TECNOLOGICO

Year 2012

30,000 t

How symptoms occur

Diseased grapevines show stunted growth, less Vigour, millerandage and low quality of berries

CENTRO TRASFERIMENTO TECNOLOGICO

CENTRO TRASFERIMENTO TECNOLOGICO

Bushy aspect

Shoot stunting

CENTRO TRASFERIMENTO TECNOLOGICO

CENTRO TRASFERIMENTO TECNOLOGICO

Chlorotic mottling and leaf deformations

Shoots malformation and abnormal branching

CENTRO TRASFERIMENTO TECNOLOGICO

CENTRO TRASFERIMENTO TECNOLOGICO

Bunch millerandage: weight loss

What causes???

CENTRO TRASFERIMENTO TECNOLOGICO

Different ideas:1. Insects bites2. Mites3. Direct damage from nematode4. Boron or microelements deficiency5. Phytoplasmosis6. Virosis

VIROSIS

But…Why virus?

Only a few induce pathological conditions relevant from an economic point in all wine-growing areas of the world

CENTRO TRASFERIMENTO TECNOLOGICO

62 Viruses recorded in grapevine!!!!

Outcome…

A metagenomic approach discovered a new grapevine trichovirus

which is named Grapevine Pinot Gris virus (GPGV) (Gianpietruzzi et al., 2012)

CENTRO TRASFERIMENTO TECNOLOGICO

Aim of project

CENTRO TRASFERIMENTO TECNOLOGICO

To define etiology and the biological feature of this disease we have started a project about:

1. FIELD MONITORING2. EPIDEMIOLOGY3. DIAGNOSIS4. VECTOR5. CONTROL

FEM , CNR and Bari University, Bologna University

Field monitoring: Materials and methods

• Screening on different varieties• Period of symptoms relief• In 2012 and 2013: monitored 521 vineyards

in 56 municipalities• Since 2010: 3 vineyard maps• Symptoms scale

CENTRO TRASFERIMENTO TECNOLOGICO

Field monitoring: Symptoms scale

CENTRO TRASFERIMENTO TECNOLOGICO

0 Healthy plant

1 Mild symptoms on some leaves

2 Clear symptoms of half canopy

3 Relevant symptoms of the whole plant

M Dead plants

B Rooted vines

Field monitoring: Results, map example

CENTRO TRASFERIMENTO TECNOLOGICO

Zablani, Mezzocorona, 2 ha (5 acres)

14

3

10

13 + 1 rv

0,26%0,30%0,34%0,09%M

10,09%8,11%4,94%1,82%3

9,47%14,30%10,46%5,55%2

3,96%7,40%5,69%4,08%1

74,25%68,68%78,07%88,46%0

1,96%1,21%0,82%0,00%B

2013201220112010

B

1

2

3

M

Field monitoring: Results, cultivar incidence

CENTRO TRASFERIMENTO TECNOLOGICO

2012: 137547 plants checked in 521vineyards

Pinot gris 114847 plants in 420 vineyards

941 PlantsWhit symptoms

total impact 0.8 %Max incidence

19 %

Traminer20689 plants

in 89 vineyards136 Plants

Whit symptoms

total impact 0.7 %Max incidence

10.3 %

Pinot noir 2179 plants in 10 vineyards

4 PlantsWhit symptoms

total impact 0.2 %Max incidence

2 %

2012 average: 0.8%

Field monitoring: Incidence: spatial distribution, 2012

CENTRO TRASFERIMENTO TECNOLOGICO

0.11%

0.91%

0.04%

Monitoring 2013

CENTRO TRASFERIMENTO TECNOLOGICO

data reworking

1.Year planted

2.Clone

3.Roostock

Interactionts????

And finally…

CENTRO TRASFERIMENTO TECNOLOGICO

And Thanks to:

Grapegrowers, for their reports and the willingness to

cooperate

Mauro Varner, who help us during monitoring

and all those who have allowed me to be here today!

THANKS FOR YOUR ATTENTION