abird presentation 2009
TRANSCRIPT
![Page 1: ABIRD Presentation 2009](https://reader033.vdocuments.net/reader033/viewer/2022051122/58a252701a28abe8738b5fc3/html5/thumbnails/1.jpg)
Active Background Ion Reduction Device (ABIRD) for nanospray ESI
ESI Source Solutionswww.esisourcesolutions.com
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Nanospray ESI (n-ESI) is a common technique for interfacing capillary chromatography systems
with a mass spectrometer.
n-ESI is typically done without supplied gas, leaving the background ion signal subject to variable
ambient laboratory air conditions.
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ABIRD works by treating the ambient lab air, which is then directed to the n-ESI spray formation zone in an open source.
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ABIRD also works by treating the ambient lab air which then displaces untreated air in a semi closed source.
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Typical Orbitrap full MS no ABIRD
7.59e5
Typical Orbitrap full MS with ABIRD.
5.00e4
ABIRD on an Orbitrap shows greater than a 10x reduction in background ions when operating.
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ABIRD installed on an LTQ shows 10x reduction in background ion signal.
2.36e3
Typical LTQ full MS with ABIRD. Typical LTQ full MS no ABIRD
2.38e4
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ABRID has been demonstrated on the Q-TOF Premier. Note expanded mass range 50-2000Da. ~20x reduction in background ion signal
Full MS on Q-TOF Premier no ABIRD Full MS on Q-TOF Premier with ABIRD
6.42e3 3.41e2
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ABIRD significantly improves signal to noise and signal stability across a chromatographic run.
ABIRD OFF
ABIRD ON
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ABIRD isolates the mass spectrometer from transient sources of background ions in the lab.
ABIRD ON
ABIRD OFF: background ion spike and continued rise.
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Front view of ABIRD device. Approved by mascot, Purdy.
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Rear and side views:
Features include Teflon FEP interface to source, lightweight plastic housing, power switch, various mounting configurations. ABIRD uses inexpensive
disposable cartridge filters instead of supplied gas.
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ABIRD on LTQ-Orbitrap with a New Objective Picoview source:
ABIRD has also been fitted to the Thermo Fisher’s OEM nanospray source, the Proxeon’s nanospray source, and Advion’s Nanomate.
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ABIRD device in place on Q-TOF Premier with Waters nanospray source.
Note the simple, clean installation which leaves room for column compartment and does not interfere with access to nanospray assembly.
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ABIRD mounted on a Proxeon LTQ source
ABIRD easily mounts to the Proxeon LTQ source, on either the left or right side of the side, with a ball and clamp mount on an extra camera arm.
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Summary:• ABIRD imparts a controlled low level background ion signal to
nanoESI mass spectrometers across a wide mass range.
• ABIRD protects the instrument from ambient spikes in laboratory air quality.
• ABIRD works with either an open or semi-sealed ESI source.
• ABIRD uses inexpensive disposable cartridge filters.
• ABIRD is adaptable to many common ESI sources.