absorbance microplate reader

Absorbance Microplate Reader

Operator’s Manual

800™ TSAbsorbance Reader

Operator's Manual

BioTek® Instruments, Inc.© August 2017PN 1561000, Rev B

NoticesBioTek® Instruments, Inc.

Highland Park, P.O. Box 998Winooski, Vermont 05404-0998 USA

All Rights Reserved

© 2017, BioTek® Instruments, Incorporated. No part of this publication may be reproduced, transcribed, ortransmitted in any form, or by any means electronic or mechanical, including photocopying and recording, for anypurpose other than the purchaser’s use without written permission of BioTek Instruments, Inc.

Trademarks

BioTek® is a registered trademark, and 800™ TS and Gen5™ are trademarks of BioTek Instruments, Inc. BioCell™ isa trademark of BioTek Instruments and is patented under U.S. patent number 5,963,318.

Microsoft®, Windows®, and Excel® are either registered trademarks or trademarks of Microsoft Corporation inthe United States and/or other countries.

All other trademarks are the property of their respective holders.

Restrictions and Liabilities

Information in this document is subject to change and does not represent a commitment by BioTek Instruments,Inc. Changes made to the information in this document will be incorporated in new editions of the publication. Noresponsibility is assumed by BioTek for the use or reliability of software or equipment that is not supplied byBioTek or its affiliated dealers.

BioTek Instruments, Inc.

ii | Notices

Contents| iii

Contents

Notices ii

Contents iii

Contact Information vi

Revision History vii

Document Conventions viii

Intended Use Statement ix

Quality Control ix

Warranty and Product Registration ix

Warnings ix

Hazards x

Precautions xi

CEMark xiii

Electromagnetic Interference and Susceptibility xiv

User Safety xv

Safety Symbols xvi

Introduction 1

Product Description 2

Package Contents 3

Optional Accessories 3

Product Support and Service 5

Installation 7

Product Registration 8

Important Information 8

1. Unpack and Inspect the Reader 8

2. Remove the Shipping Hardware 9

3. Install the Optional Door in Top Cover 10

4. Verify the Filter Wheel Contents 11

5. Select an Appropriate Location 14

800 TS Operator's Manual

6. Install the Power Supply 15

7. (Optional) Install the Printer 15

8. (Optional) Install Gen5 on the Host Computer 16

9. (Optional) Install the USB Driver 17

10. (Optional) Connect the Host Computer 17

11. Turn on the Reader 17

12. Set Date and Time on Touchscreen 18

13. Verify the Reader's Filter Table 18

14. (Optional) Establish Communication 19

15: Run a System Test 20

Operational/Performance Qualification 23

Repackaging and Shipping Instructions 24

Getting Started 27

External Components 28

Operate the Reader Using the Touchscreen 31

Operate the Reader Using Gen5 Software 38

Recommendations for Optimum Performance 41

Maintenance 43

Maintenance Overview 44

Warnings and Precautions 45

Clean Exposed Surfaces 45

Clean the Touchscreen 46

Decontamination 47

Filter Storage and Handling 49

Replacing and Aligning the Bulb 49

Instrument Qualification Process 51

Instrument System Test 52

Absorbance Testing 52

Instrument Qualification Procedures 61

Overview 62


iv | Contents

Contents| v

IQ/OQ/PQ Description 62

Recommended Qualification Schedule 64

System Test 64

Absorbance Plate Test 66

Absorbance Liquid Tests 68

Specifications 75

General Specifications 76

Absorbance Specifications 77

Error Codes 81

Error Codes Overview 82

Error Codes 82

Sample Reports 89

Sample System Report 90

Sample Absorbance Test Plate Report 91

Sample Assay Results 92


Contact InformationBioTek® Instruments, Inc.Highland Park, P.O. Box 998Winooski, Vermont 05404-0998 USA

Global Service and SupportBioTek instrument service and repair is available worldwide at one of BioTek'sInternational Service Centers and in the field at your location. To arrange for service orrepair of your instrument, contact the office nearest you; visit www.biotek.com for up-to-date contact information. For customer service, sales, and technical assistance, refer to theinformation below.

Customer Service and Sales

Internet: www.biotek.com

Phone: 888-451-5171 (toll-free in the U.S.)

802-655-4740 (outside the U.S.)

Fax: 802-655-7941

Email: [email protected]

Service/Technical Assistance Center (TAC)

Phone: 800-242-4685 (toll-free in the U.S.)

802-655-4740 (outside the U.S.)

Fax: 802-654-0638


European Coordination Center/Authorized European Representative

BioTek® Instruments GmbHKocherwaldstrasse 34D-74177 Bad FriedrichshallGermany

Internet: www.biotek.de

Phone: +49 (0) 7136 9680

Fax: +49 (0) 7136 968 111



vi | Contact Information

http://www.biotek.com/


http://www.biotek.de/

Revision History| vii

Revision History

Rev Date Changes

A 5/2017 Release to production

B 8/2017 Preface and Installation chapter: Deleted the word “ambient” when describing specifiedtemperature ranges; corrected Fahrenheit value of the low end of the specifiedtemperature range.Getting Started: Added note about the included blank USB stick.Specifications:Added the Fahrenheit temperature values.


Document Conventions

This icon calls attention to important safety notes.

Warning!AWarning indicates the potential for bodily harm and tells you how to avoid theproblem.

CautionA Caution indicates potential damage to the instrument and tells you how to avoid theproblem.

Note Bold text is primarily used for emphasis.

italicTopics that apply only to specific 800 TS models are preceded by a notice in italic, forexample, Applies only to 800 TS models with incubation.

This icon calls attention to important information.


viii | Document Conventions

Intended Use Statement| ix

Intended Use StatementThe 800 TS is a single-channel, filter-based absorbance reader designed to performmeasurements of samples in a microplate format. The performance characteristics of thedata reduction software have not been established with any laboratory diagnostic assay.Users must evaluate this instrument and PC-based software in conjunction with theirspecific assay(s). This evaluation must include the confirmation that performancecharactertistics for the specific assay(s) are met.

Quality ControlIt is considered good laboratory practice to run laboratory samples according toinstructions and specific recommendations included in the assay package insert for the testto be conducted. Failure to conduct Quality Control checks could result in erroneous testdata.

Warranty and Product RegistrationPlease take a moment to review the warranty information that shipped with your product.Please also register your product with BioTek to ensure that you receive importantinformation updates about the product(s) you have purchased.You can register online through the Customer Resource Center (CRC) at www.biotek.comor by calling 888-451-5171 or 802-655-4740.

Warnings

Operate the instrument on a level, stable surface away from excessive humidity.

Bright sunlight or strong incandescent light can reduce the linear performancerange of the instrument.

Measurement values may be affected by extraneous particles (such as dust) in themicroplate wells. A clean work area is necessary to ensure accurate readings.

When operated in a safe environment according to the instructions in thisdocument, there are no known hazards associated with the instrument. However,the operator should be aware of certain situations that could result in seriousinjury; these may vary depending on the instrument model. See Hazards andPrecautions.


HazardsThe following hazards are provided to help avoid injury:

Warning! Power Rating. The instrument’s power supply or power cord mustbe connected to a power receptacle that provides voltage and current withinthe specified rating for the system. Use of an incompatible power receptaclemay produce electrical shock and fire hazards.

Warning! Electrical Grounding. Never use a plug adapter to connect primarypower to the external power supply. Use of an adapter disconnects the utilityground, creating a severe shock hazard. Always connect the power corddirectly to an appropriate receptacle with a functional ground.

Warning! Service. Only qualified technical personnel should perform serviceprocedures on internal components.

Warning! Accessories. Only accessories that meet the manufacturer'sspecifications shall be used with the instrument.

Warning! Lubricants. Do not apply lubricants to the microplate carrier orcarrier track. Lubricant on the carrier mechanism or components in thecarrier compartment will attract dust and other particles, which may obstructthe carrier path and cause the instrument to produce an error.

Warning! Liquids. Avoid spilling liquids on the instrument; fluid seepage intointernal components creates a potential for shock hazard. If a spill occurswhile a program is running, abort the program and turn off the instrument.Wipe up all spills immediately. Do not operate the instrument if internalcomponents have been exposed to fluid. Contact BioTek TAC for assistance.

Warning! Unspecified Use. Failure to operate the equipment according to theguidelines and safeguards specified in this manual could result in a hazardouscondition.

Warning! Software Quality Control. The operator must follow themanufacturer’s assay package insert when modifying software parametersand establishing reading methods. Failure to conduct quality control checkscould result in erroneous test data.

Warning! Reader Data Reduction Protocol. No limits are applied to the rawmeasurement data. All information exported via computer control must bethoroughly analyzed by the operator.


x | Hazards

Precautions| xi

Warning! Hot Surface.The tungsten lamp assembly is hot when theinstrument is turned on. Turn off the reader and allow the lamp to cool for atleast 15 minutes before attempting to replace it.

Warning! Internal Voltage. Always turn off the power switch and unplug thepower supply before cleaning the outer surface of the instrument.

Warning! Potential Biohazards. Some assays or specimens may pose abiohazard. This hazard is noted by the symbol shown here. Adequate safetyprecautions should be taken as outlined in the assay’s package insert. Alwayswear safety glasses and appropriate protective equipment, such as chemical-resistant rubber gloves and apron.

PrecautionsThe following precautions are provided to help avoid damage to the instrument:

Caution: Service. The instrument should be serviced by BioTek-authorizedservice personnel. Only qualified technical personnel should perform serviceprocedures on internal components.

Caution: Spare Parts. Only approved spare parts should be used formaintenance. The use of unapproved spare parts and accessories may result ina loss of warranty and potentially impair instrument performance or causedamage to the instrument.

Caution Touchscreen. Do not use sharp implements to operate thetouchscreen. Using a sharp stylus or other implement may damage the display.

Caution: Environmental Conditions. Do not expose the system to temperatureextremes. For proper operation, the temperature near the instrument shouldremain within the range listed in Appendix A, Specifications. Performance maybe adversely affected if temperatures fluctuate above or below this range.

Caution: Sodium Hypochlorite. Do not expose any part of the instrument to therecommended diluted sodium hypochlorite solution (bleach) for more than 20minutes. Prolonged contact may damage the instrument surfaces. Be certain torinse and thoroughly wipe all surfaces.

Caution: Power Supply. Use only the power supply shipped with the instrument.Operate this power supply within the range of line voltages listed on it.


Caution: Shipping Hardware.The shipping hardware must be removed beforeoperating the instrument and reinstalled before repackaging the instrument forshipment.

Caution: Disposal. Dispose of the instrument according to Directive2012/19/EU, “on waste electrical and electronic equipment (WEEE)” or localordinances.

Caution: Warranty. Failure to follow maintenance protocols may void thewarranty. See Chapter 4, Maintenance.

Caution: Electromagnetic Environment. Per IEC 61326-2-6 it is the user’sresponsibility to ensure that a compatible electromagnetic environment for thisinstrument is provided and maintained in order that the device will perform asintended.

Caution: Electromagnetic Compatibility. Do not use this device in closeproximity to sources of strong electromagnetic radiation (e.g., unshieldedintentional RF sources), because these may interfere with the proper operation.


xii | Precautions

CE Mark| xiii

CE Mark

Based on the testing described below and informationcontained herein, this instrument bears the CE mark

Refer to the Declaration of Conformity for more specific information.

Directive 2014/30/EU: Electromagnetic Compatibility

Emissions—Class A

The system has been type-tested by an independent, accredited testing laboratory andfound to meet the requirements of EN 61326-1: Class A for Radiated Emissions and LineConducted Emissions.Verification of compliance was conducted to the limits and methods of EN 55011 – (CISPR11) Class A. In a domestic environment it may cause radio interference, in which case youmay need to mitigate the interference.

Immunity

The system has been type-tested by an independent, accredited testing laboratory andfound to meet the requirements of EN 61326-1 and EN 61326-2-6 for Immunity.Verification of compliance was conducted to the limits and methods of the following:EN 61000-4-2, Electrostatic DischargeEN 61000-4-3, Radiated EM FieldsEN 61000-4-4, Electrical Fast Transient/BurstEN 61000-4-5, Surge ImmunityEN 61000-4-6, Conducted Disturbances from RFIEN 61000-4-11, Voltage Dips, Short Interruptions and Variations

Directive 2014/35/EU Low Voltage (Safety)The system has been type-tested by an independent testing laboratory and was found tomeet the requirements of this Directive. Verification of compliance was conducted to thelimits and methods of the following:EN 61010-1. "Safety requirement for electrical equipment for measurement, control andlaboratory use. Part 1, General requirements."EN 61010-2-081. “Particular requirements for automatic and semi-automatic laboratoryequipment for analysis and other purposes.”EN 61010-2-010. “Particular requirements for laboratory equipment for the heating ofmaterials.“


Directive 2012/19/EU: Waste Electrical and Electronic EquipmentDisposal Notice: Dispose of the instrument according to Directive 2012/19/EU, “on wasteelectrical and electronic equipment (WEEE)” or local ordinances.

Directive 98/79/EC: In Vitro Diagnostics (if labeled for this use)

l Product registration with competent authorities

l EN 61010-2-101. “Particular requirements for in vitro diagnostic (IVD) medicalequipment.”

l Traceability to the U.S. National Institute of Standards and Technology (NIST).

Electromagnetic Interference and Susceptibility

USA FCC CLASS ARADIO AND TELEVISION INTERFERENCENOTE: This equipment has been tested and found to comply with the limits for a ClassA digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to providereasonable protection against harmful interference when the equipment is operated in acommercial environment. This equipment generates, uses, and can radiate radio frequencyenergy and, if not installed and used in accordance with the instruction manual, may causeharmful interference to radio communications. Operation of this equipment in aresidential area is likely to cause harmful interference, in which case the user will berequired to correct the interference at their own expense.In order to maintain compliance with FCC regulations, shielded cables must be used withthis equipment. Operation with non-approved equipment or unshielded cables is likely toresult in interference to radio and television reception.

Canadian Department of Communications Class AThis digital apparatus does not exceed Class A limits for radio emissions from digitalapparatus set out in the Radio Interference Regulations of the Canadians Department ofCommunications.Le present appareil numerique n'emet pas du bruits radioelectriques depassant les limitesapplicables aux appareils numerique de la Class A prescrites dans le Reglement sur lebrouillage radioelectrique edicte par le ministere des Communications du Canada.


xiv | Electromagnetic Interference and Susceptibility

User Safety| xv

User SafetyThis device has been type-tested by an independent laboratory and found to meet therequirements of the following:

l Underwriters Laboratories UL 61010-1, “Safety requirements for electrical equipmentfor measurement, control and laboratory use; Part 1: General requirements.”

l Canadian Standards Association CAN/CSA C22.2 No. 61010-1, “Safety requirementsfor electrical equipment for measurement, control and laboratory use; Part 1:General requirements.”

l EN 61010 Standards, see CE Mark starting on page xiii.


Safety SymbolsSome of the following symbols may appear on the instrument or accessories:

Alternating currentCourant alternatifWechselstromCorriente alternaCorrente alternata

Warning, risk of crushing or pinchingAttention, risque d'écrasement et pincementWarnen, Gefahr des Zerquetschens undKlemmenPrecaución, riesgo del machacamiento ysejeciónAttenzione, rischio di schiacciare edintrappolarsi

Direct currentCourant continuGleichstromCorriente continuaCorrente continua

Warning, hot surfaceAttention, surface chaudeVorsicht, heiße OberflächePrecaución, superficie calienteAttenzione, superfice calda

Both direct and alternatingcurrentCourant continu et courantalternatifGleich - und WechselstromCorriente continua ycorriente alternaCorrente continua ecorrente alternata

Laser radiation: Do not stare into beamRayonnement laser: Ne pas regarder dans lefaisceauLaserstrahlung: nicht in den strahl blickenRadiación de laser: No mire fijamente al rayoRadiazione di laser: Non stare nel fascio

Earth ground terminalBorne de terreErde (Betriebserde)Borne de tierraTerra (di funzionamento)

Warning, potential biohazardsAttention, risques biologiques potentielsWarnung! Moegliche biologische GiftsoffeAtención, riesgos biológicosAttenziones, rischio biologico

Protective conductorterminalBorne de terre deprotectionSchultzleiteranschlussBorne de tierra deprotecciónTerra di protezione

Caution (refer to accompanying documents)Attention (voir documentsd'accompanement)Achtung siehe BegleitpapiereAtención (vease los documentos incluidos)Attenzione, consultare la doc annessa


xvi | Safety Symbols

Safety Symbols| xvii

On (Supply)Marche (alimentation)Ein (Verbindung mit demNetz)ConectadoChiuso

Consult instructions for useConsulter la notice d'emploiGebrauchsanweisung beachtenConsultar las instrucciones de usoConsultare le istruzioni per uso

Off (Supply)Arrêt (alimentation)Aus (Trennung vom Netz)DesconectadoAperto (sconnessione dallarete di alimentazione)

In vitro diagnostic medical deviceDispositif médical de diagnostic in vitroMedizinisches In-Vitro DiagnostikumDispositivo médico de diagnóstico in vitroDispositivo medico diagnostico in vitro

Warning, risk of electricshockAttention, risque de chocélectriqueGefährliche elektrischeschlagPrecaución, riesgo desacudida eléctricaAttenzione, rischio di scossaelettrica

Separate collection for electrical andelectronic equipmentLes équipements électriques et électroniquesfont l'objet d'une collecte sélectiveGetrennte Sammlung von Elektro- undElektronikgerätenRecogida selectiva de aparatos eléctricos yelectrónicosRaccolta separata delle apparecchiatureelettriche ed elettroniche



xviii | Safety Symbols

Chapter 1

Introduction

This chapter introduces the 800 TS and provides contact information fortechnical assistance.

Product Description 2

Package Contents 3

Optional Accessories 3

Product Support and Service 5

2 | Chapter 1: Introduction

Product DescriptionThe 800 TS is a compact, filter-based, single-channel absorbance microplate reader. Allmodels are equipped with a touchscreen interface and support endpoint dual-wavelengthreads from 400-750 nm on standard 6- to 96-well plates. The narrow beam (NB) modelsupports 384-well plates. UV models support a measurement range from 340-750 nm.Some models offer incubation to 50˚C and/or linear plate shaking.Basic data analysis, reporting, and exporting are provided via the touchscreen. Withoptional Gen5 software, kinetic and well area scanning read modes are supported, alongwith BioCell and 60-, 72-, and 96-well Terasaki plates. Gen5 also offers extensive dataanalysis and reporting and exporting capabilities. The reader is available in five models.

Model Measurement Range Default Filters (in nm) Plate Types Shaking Incubation

800TS 400-750 nm 405, 450, 490, 630 6- to 96-well yes no

800TSI 400-750 nm 405, 450, 490, 630 6- to 96-well yes yes

800TSNB 400-750 nm 405, 450, 490, 630 6- to 384-well no no

800TSUV 340-750 nm 340, 405, 450, 490, 630 6- to 96-well yes no

800TSUVI 340-750 nm 340, 405, 450, 490, 630 6- to 96-well yes yes

The filter wheel contains up to five filters and is user accessible. For models with fewerthan five filters, removable plugs are installed in the empty wheel locations.The 800TS and 800TSI models use a tungsten bulb as their light source. The other models,800TSUV, 800TSUVI, and 800TSNB, use a halogen bulb.

Use of labware other than those described here can result in positioning errors during programexecution.

Models with incubation capability are equipped with a door. This door is also availablefor purchase as an accessory for non-incubation-capable models. If you order a doorseparately, you must install it yourself.

See Appendix A, Specifications, for performance and technical specifications.


Package Contents | 3

Package ContentsPackage contents and part numbers are subject to change. Please contact BioTek CustomerCare with any questions.

Item Part #

800 TS Operator's Manual on USB flash drive 1561000

Power supplyNon-incubation models: 01281Incubation models: 02395

Power cord varies according to country of use

USB cable 75108

Blank 4 GB USB flash drive 01087

Dust cover 7332040

Gen5 RC software GEN5RC

Optional Accessories

Accessory availability and part numbers are subject to change. Please contact BioTekCustomer Care if you have any questions or visit www.biotek.com and use theAccessories search tool.

Item Part #

7-filter Absorbance Test Plate for absorbancemeasurement testing

7260522

Absorbance Test Plate for absorbancemeasurement testing at 340 nm

7260551

800 TS Product Qualification (IQ-OQ-PQ)package

1560516

Accessory door for top cover 1560013

RP-D10 Seiko thermal printer 02434

Power cord for printer varies according to country of use

BioCell quartz vessel 7272051




Item Part #

BioCell adapter plate (holds up to 8 BioCells) 7270512

Terasaki adapter plate 7330531

Cuvette holder 7302030

Tungsten replacement lamp kit for 800TS and800TSI

1500532

Halogen replacement lamp kit for 800TSUV,800TSUVI, and 800TSNB

1560533

Filters7334 + wavelength (e.g., 7334405,7334540)

Gen5 software/upgradeVisit www.biotek.com or contact yourlocal dealer for details

Materials for Liquid Tests Part #

BioTek Wetting Agent Solution (PN 7773002) 7773002

BioTek QC Check Solution No. 1 (25 mL) orBioTek QC Check Solution No. 1 (125 mL)

71207797120782

β-NADH Powder (β-Nicotinamide Adenine Dinucleotide,Reduced Form)

Sigma #N6785-10VL (or BioTekPN 98233)

Phosphate-Buffered Saline (PBS) Tablets (pH 7.2–7.6) Sigma #P4417



Product Support and Service | 5

Product Support and Service

See page vi for contact information for BioTek Global Service and Support.

Technical Assistance Center (TAC)If your instrument or software fail to function properly, if you have questions about how touse or maintain our products, or if you need to send an instrument to BioTek for service orrepair, please contact our Technical Assistance Center (“TAC”).TAC is open from 8:30 AM to 5:30 PM (EST), Monday through Friday, excluding standardU.S. holidays.

l Phone: (800) 242-4685 or (802) 655-4740

l Fax: (802) 654-0638

l E-Mail: [email protected]

l Web: www.biotek.com

Please be prepared to provide the following information:

l Your name and company information, along with a daytime phone or fax number,and/or an e-mail address

l The product name, model, and serial number (the serial number is located on theright side of the reader)

l The onboard software part number and basecode version

l Via the touchscreen by tapping Instrument on the Main Menu.

l Via Gen5 for the 800 TS by selecting System > Instrument Configuration,select 800 TS, then click View/Modify > Setup, select the Basecode tab, andclick Get Basecode Information

l For troubleshooting assistance or instruments needing repair, the specific steps thatproduce your problem and any error codes displayed on the touchscreen or in Gen5(see also Appendix B, Error Codes)

l Gen5 users: A text file of the diagnostic history of the instrument (select System> Diagnostics > History, then select the appropriate file and click Export)



Running a system test when a problem occurs provides valuable information for TAC.When the test is complete, save it from the touchscreen to a USB flash drive or, inGen5, click Save As to save a text file of the system test report, which can be emailedto TAC.

If you need to return an instrument to BioTek for service or repair, please contact the TACfor a Service Call Notice (SCN) number and the shipping address. Repackage theinstrument according to the instructions at the end of Chapter 2, Installation.


Chapter 2

Installation

This chapter includes instructions for unpacking and setting up the 800 TS.Instructions are also included for preparing the reader for shipment.

Product Registration 8

Important Information 8

1. Unpack and Inspect the Reader 8

2. Remove the Shipping Hardware 9

3. Install the Optional Door in Top Cover 10

4. Verify the Filter Wheel Contents 11

5. Select an Appropriate Location 14

6. Install the Power Supply 15

7. (Optional) Install the Printer 15

8. (Optional) Install Gen5 on the Host Computer 16

9. (Optional) Install the USB Driver 17

10. (Optional) Connect the Host Computer 17

11. Turn on the Reader 17

12. Set Date and Time on Touchscreen 18

13. Verify the Reader's Filter Table 18

14. (Optional) Establish Communication 19

15: Run a System Test 20

Operational/Performance Qualification 23

Repackaging and Shipping Instructions 24

8 | Chapter 2: Installation

Product RegistrationPlease register your product(s) with BioTek to ensure that you receive importantinformation and updates about the product(s) you have purchased.Register online through BioTek’s Customer Resource Center (CRC) at www.biotek.com orby contacting BioTek Customer Care at (888) 451-5171 or (802) 655-4740.

Important Information

This chapter contains installation tasks for the 800 TS and accessories. Performthe tasks in the order presented, skipping those that do not apply to your reader’sconfiguration.

Remove the shipping hardware before turning on the instrument.

Reinstall the shipping hardware before repackaging the instrument for shipment.

1. Unpack and Inspect the Reader

Save all packaging materials. If you need to ship the reader to BioTek for repair orreplacement, you must use the BioTek-supplied materials. Using other forms ofcommercially available packaging, or failing to follow the repackaging instructions,may void your warranty.

During the unpacking process, inspect the packaging, reader, and accessories forshipping damage. If the reader is damaged, notify the carrier and your BioTekrepresentative. Keep the shipping boxes and the packaging materials for the carrier'sinspection. BioTek will arrange for repair or replacement immediately.

1. Open the shipping box, remove the accessories tray, and then remove the instrumentfrom the box and place it on a level, stable surface.

2. Place the packaging materials back into the shipping box for reuse if the instrumentneeds to be shipped again.


2. Remove the Shipping Hardware | 9

2. Remove the Shipping Hardware

Remove and store all shipping hardware before you turn on the reader.

1. Carefully turn the reader upside down on a level surface.

2. Remove the six black screws holding the cover to the base, then lift the base off thecover and set it right side up on a level surface.

3. Remove the two screws on each of the shipping brackets, remove the brackets, andstore the screws in the holes on the brackets.



4. Store the shipping hardware in a safe place in case it is needed in the future.

5. If applicable (described in the next section), install the door now.

3. Install the Optional Door in Top Cover

Applies to models for which the door was ordered as a separate accessory.

1. If you have not already done so, remove the top cover by carefully turning over thereader and removing the six screws that attach the top cover to the base.

2. Remove the two hole plugs from the top cover. You do not need to retain these plugs.

3. Using the nuts and washers that ship with the door, attach the door to the top cover asshown next.


4. Verify the Filter Wheel Contents | 11

If you have installed the door on an existing reader (i.e., outside of the instrumentinstallation process), you can reattach the top cover now. Otherwise, leave the topcover off and continue to 4. Verify the Filter Wheel Contents.

4. Tape the door closed, then carefully turn the reader upside down over its cover, andreattach the cover to the reader by screwing in the six screws.

4. Verify the Filter Wheel ContentsThe 800 TS ships with up to five preordered filters in the filter wheel. During installation, itis good practice to verify the filters and their placement, and to confirm that the softwarefilter table matches the filter wheel’s configuration (discussed later). All five locations inthe wheel must contain either a filter or a plug.Before continuing, obtain a clean, lint-free cloth.



For models with incubation capability: You must first remove the incubation housing toaccess the filter wheel.

1. With the top case removed from the reader (see Remove the Shipping Hardwareon page 9 for directions), remove the four thumbscrews from the optic arm, and liftthe optic arm cover off (if equipped).

2. Disconnect the two connectors on the back of the incubation housing, then gently liftthe housing off of the reader.


4. Verify the Filter Wheel Contents | 13

To access the filter wheel:

1. With the top case removed from the reader(see Remove the Shipping Hardware on page 9for directions), remove the four thumbscrewsfrom the filter wheel cover. This cover is directlyunder the optics arm.

Note: The filters are not held in place and fall out of the wheel easily, so make sure to have a clean, lint-free cloth in place before turning over the filter wheel.

2. Lift the filter wheel off its pin, then removethe filters by turning the wheel upside downover a clean, lint-free cloth. Write down whichfilters are installed in the filter wheel and thelocation of each filter. You will use thisinformation later to verify/edit the softwarefilter table.

Compare the filters with your model’s defaultfilters (see the table on page 2) or with thepurchase order if different filters were ordered.Contact BioTek Customer Care if you did notreceive the expected filters/plugs.

Each filter is labeled with a wavelength and anarrow showing the light direction. The arrowsmust point downward when the filter wheel isinstalled on the reader.



3. When you are finished examining the filters,replace the filters in the filter wheel, beingcareful to insert them in the correct direction(indicated by the arrow printed on the side ofeach filter).

4. Replace the filter wheel on its pin, and thenreplace the filter wheel cover using the fourthumbscrews removed during step 1.

5. Tape the door closed, if equipped.

6. Carefully turn the reader upside down over itscover, and reattach the reader to the top coverby replacing the six screws.

5. Select an Appropriate LocationInstall the reader on a level, stable surface. Select an area where temperatures between18°C (64°F) and 40°C (104°F) can be maintained.The reader is sensitive to extreme environmental conditions. Avoid the following:

l Excessive humidity. Condensation directly on the sensitive electronic circuits cancause the instrument to fail internal self-checks. The humidity must be in the range of10–85%, non-condensing.

l Excessive ambient light. Bright light may affect the reader’s optics and readings,reducing its linear range.

l Dust. Readings may be affected by extraneous particles (such as dust) in themicroplate wells. A clean work area is necessary to ensure accurate readings.


6. Install the Power Supply | 15

6. Install the Power Supply

Power Rating. The instrument must be connected to a power receptacle thatprovides voltage and current within the specified rating for the system. Use of anincompatible power receptacle may produce electrical shock and fire hazards.

Electrical Grounding. Never use a plug adapter to connect primary power to theinstrument. Use of an adapter disconnects the utility ground, creating a severeshock hazard. Always connect the system power cord directly to an appropriatereceptacle with a functional ground.

1. Connect the power cord to the external power supply.

2. Locate the power inlet on the rear of the reader.

3. Plug the rounded end of the power supply's cord into the power inlet.

4. Plug the other end of the power cord into an appropriate power receptacle.

7. (Optional) Install the Printer

Unpack the Printer

1. Open the printer’s shipping box, and remove the top foam tray.

2. Carefully remove the printer and its components from the shipping box.

Note that the printer is shipped with two adapter plates for use with 58 mm paper.Unless you plan to use that size of paper roll, you do not need to use these adapterplates.



Install the Printer

1. Insert the round end of the power supply cord into the DC power port on the rear of theprinter, then attach one end of the power cord to the power supply box and plug theother end into a power outlet.

2. Insert one end of the supplied USB cable into the USB printer port on the rear of theinstrument and the other end in the USB port on the printer.

Do not use the USB port on the front of the 800 TS; that port is for USB flash drivesonly.

8. (Optional) Install Gen5 on the Host Computer

There is a certain sequence of events thatmust be followed to ensure that thesoftware is properly installed and configured. Please follow the instructionsprovided in Gen5 Getting Started Guide to install the software.


9. (Optional) Install the USB Driver | 17

9. (Optional) Install the USB Driver

Refer to the instructions that shipped with the Gen5 software to install the necessarydrivers. The driver must be installed on the computer before you connect theinstrument.

10. (Optional) Connect the Host Computer

The USB port is located on the rear of the reader.

1. Turn off the computer. If the reader is on, turn it off.

2. Using the supplied USB cable, connect the square end of the cable to the USB port onthe back of the reader.

3. Connect the other end of the cable to an available USB port on the computer.

11. Turn on the Reader

1. Locate the power on/off switch on the right side of the instrument, and turn on thereader. The reader delays any action until the bulb has warmed up: three minutes fornarrow beam and UV models, and 30 seconds otherwise. Then the reader performs apower-up system test.

If using Gen5, do not attempt to communicate with the reader until the systemtest is finished.

2. When the system test is completed, the touchscreen displays its main screen.

If an error occurs during the system test, the reader beeps and the error code is displayedon the touchscreen. Make note of the error code number, then tap OK to stop the beeping.See Appendix B, Error Codes, for information about the error code you noted.



12. Set Date and Time on TouchscreenThe date and time are included in the instrument’s system test report. Define thesesettings now to ensure correct information when you perform step 15.

1. From theMain Menu, tap Instrument, the Config tab,then the Time button.

2. Tap the hour value, and use the keypad to enter thecorrect time for both the hour and minutes, then click OK.

Note: You can change just theminutes value: Tap theminutes value and enter the correct time.

3. Tap theDate button.

4. Tap month, day, or year, and use the keypad to set thecurrent date. Tap OK when done.

13. Verify the Reader's Filter TableBefore using the 800 TS, verify that the filter table reflects the filters installed in the filterwheel.

Important! The reader’s filter table must exactly match the contents of the installedfilter wheel.


14. (Optional) Establish Communication | 19

1. From theMain Menu, tap Instrument, the Config tab,and then Filter Table. Filter wheel locations 1 through 5are shown, with each location’s filter value (in nm) or“plug” for a blank filter.

2. Verify that the values match the contents of the filterwheel. See the Installation chapter for instructions foraccessing the filter wheel.

3. To change the setting for a filter wheel position, tap itsvalue and use the keypad to enter a wavelength value (innm), or select Plug. Tap OKwhen finished.

4. If you made any changes, tap Save in the Installed Filtersscreen. The filter values are now available for selection inprotocols and the absorbance test plate setup screen.

See the values you wrote down in step 2 of "4. Verify the Filter Wheel Contents" onpage 11. If there is a mismatch, correct the Filter Table to match the filters in the filterwheel. See "Verify or Change the Reader’s Filter Table" on page 33 for instructions.

14. (Optional) Establish Communication

NOTE: Instrument must be at the Main Menu screen for Gen5 communication to occur.

1. On the host computer, start Gen5 and log in if prompted. The default SystemAdministrator password is admin.

2. From the Gen5 main screen, select System > Instrument Configuration and click Add.

3. Set the Reader Type to 800 TS.

4. Perform one of the following steps, as applicable:

l Select Plug & Play.

An 800 TS must be connected via USB to the computer and turned on to appearin the Available Plug & Play Readers list.

l Set the Com Port to the computer's COM port to which the reader is connected.



The information can be found via the Windows Control Panel, under Ports in theHardware/Device Manager area of System Properties (e.g., Serial Port (COM5)).

5. To verify that Gen5 can communicate with the instrument, click Test Comm. If thecommunication attempt is successful, Gen5 displays a success message. Return toGen5’s main screen.

Communication ErrorsIf the communication attempt is not successful, try the following:

l Is the reader connected to the power supply and turned on?

l Is the communication cable firmly attached to both the reader and the computer?

l Did you select the correct Reader Type in Gen5?

l Try a different COM Port in Gen5 or use Plug & Play.

l Did you install the USB driver software?

l Is the touchscreen at the Main Menu?

If you remain unable to get Gen5 and the reader to communicate with each other,contact BioTek’s Technical Assistance Center.

15: Run a System TestRunning a system test will confirm that the reader is functioning properly, or will providean error code if a problem is detected.Instructions for performing the test using Gen5 are provided on page 22.

Using the Touchscreen

1. If the reader is equipped with an incubator, turn it on.

a. On the Main Menu, tap the temperature display (circled in the following image).When the incubator is turned on, the dashes are replaced by the reader’s internaltemperature, in degrees Celsius.


15: Run a System Test | 21

b. On the Incubate tab of the Quick Menu screen, turn on the Temperature Control andenter a setpoint of at least 37°C, then tap Home to return to the Main Menu.

c. Wait for the temperature display to reach the defined setpoint before continuing.

2. From the Main Menu, tap Instrument > Options.



3. Under System test, tap Start.

4. When the test finishes, tap USB Report to save the test results to a USB flash drive,Print to print the test results, or Exit to close the screen.

5. If applicable, turn off incubator.

Using Gen5

1. If necessary, launch Gen5 and turn on the incubator:

l From the Gen5 main screen, select System > Instrument Control > 800 TS.

l Click the Pre-Heating tab.

l Enter a Requested temperature of at least 37°C and click On.

l Wait until the incubator temperature reaches the set point before continuing.

2. Return to Gen5’s main view and select System > Diagnostics > Run System Test. Ifprompted to select a reader, select 800 TS and click OK.

3. If a message appears, stating that the reader has a pending system test report, click OK,then click Close.

The reader ran a "power-up" system test, but that test did not include verifyingthat the incubator reaches a set temperature. Therefore, you will run anothersystem test.

Again, select System > Diagnostics > Run System Test. If prompted to select a reader, select 800TS and click OK.

4. When the test is completed, a dialog requesting additional information appears. Enterthe information and click OK.

5. The results report appears and should contain the text "SYSTEM TEST PASS."

l If required, print the report and store it with your records.

l The Gen5 software stores system test information in its database; you can retrieveit at any time.

l You can save the system test report as a text file: click Save As in the System TestResults dialog.


Operational/Performance Qualification | 23

If an error code is returned, refer to Appendix B and look up the code. If theproblem is something you can fix, do so now and run another system test. If theproblem is something you cannot fix, or if the test continues to fail, contactBioTek’s Technical Assistance Center at (800) 242-4685 or (802) 655-4740.

6. If applicable, turn off the incubator:

l Select System > Instrument Control > 800 TS.

l Click the Pre-Heating tab and click Off.

l Return to Gen5’s main view.

Operational/Performance QualificationYour 800 TS was fully tested at BioTek prior to shipment and should operate properlyfollowing the successful completion of the installation and setup procedures described inthis chapter.If you suspect that problems occurred during shipment, if you received the reader backfrom BioTek following service or repair, or if regulatory requirements dictate thatOperational/Performance Qualification is necessary, turn to Chapter 5, InstrumentQualification now to learn about BioTek’s recommended OQ/PQ procedures for 800 TS.

A Product Qualification & Maintenance (IQ/OQ/PQ) package for the 800 TS is availablefor purchase (PN 1560516). Contact your local BioTek dealer for more information.



Repackaging and Shipping InstructionsImportant! Please read all of the information provided below before preparing the 800 TS forshipment.

Contact BioTek’s Technical Assistance Center for a service call notice (SCN)number and the shipping address before returning equipment for service.

If the reader has been exposed to potentially hazardous material, decontaminateit to minimize the risk to all who come in contact with the reader during shipping,handling, and servicing. Decontamination prior to shipping is required by the U.SDepartment of Transportation regulations. See the As-Needed Maintenancechapter for decontamination instructions.

Remove any labware from the carrier before shipment. Spilled fluids cancontaminate the optics and damage the instrument.

The instrument’s packaging design is subject to change. If the instructions in thissection do not appear to apply to the packaging materials you are using, pleasecontact BioTek’s Technical Assistance Center for guidance.

Replace the shipping hardware before repackaging the instrument. Pleasecontact BioTek if you need to replace any of these items:

l Carrier shipping bracket (PN 1560517)

l Carrier shipping bracket screws (PN 19668)

l Shaft shipping bracket (PN 7332041)

l Shaft shipping bracket screws (PN 19337)

l If applicable, top incubation shipping bracket (PN 1562072)If you need to ship the 800 TS to BioTek for service or repair, be sure to use theBioTek-supplied packaging materials. Other forms of commercially availablepackaging are not recommended and can void the warranty.

If the packaging materials have been damaged or lost, or if the same set hasbeen used more than four times, contact BioTek to order replacement partnumber 1563000. The shipping box, accessories box, and foam trays are includedas a whole set under this part number and cannot be ordered separately.


Repackaging and Shipping Instructions | 25

Attach the Shipping Hardware

The shipping brackets must be reattached before the 800 TS can be shipped.

1. Turn off the reader, and unplug the power supply from the power outlet and from thepower supply port on the back of the reader.

2. Disconnect the USB cable(s) from the reader (if using a computer and/or printer).

3. Carefully turn the reader upside down on a level surface.

4. Remove the six black screws holding the top cover to the base, then lift the base off ofthe cover and set it right side up on a level surface.

5. Place the carrier shipping bracket around the carrier rail first, then rotate the bracketdown so that it sits on the base pan.

6. Hold the carrier shipping bracket snug against the front of the carrier, then screw in thetwo screws to secure it.

7. Place the shaft shipping bracket over the shaft, and, for non-incubation-capable readers,secure it with the two screws.

8. For incubation-capable readers, place the top incubation shipping bracket over the shaftshipping bracket, and secure the brackets with two screws.



Repackage the InstrumentEnsure that the shipping hardware has been attached as instructed in the previous section.Refer to the next two figures when performing the following steps:

1. Place the bottom foam tray into the bottom of the shipping container.

2. If the reader has a door, tape it shut, then place the reader inside the original plasticbag, and carefully lower the reader into the foam tray in the bottom of the box.

3. Place the accessory tray in the box, and then place the accessories into the tray asshown in the figure.

4. Close the top of the box, and secure it with shipping tape.

5. Write the service call notice (SCN) number and shipping address in large, clear letterson the outside of the shipping container, and ship the box to BioTek.


Chapter 3

Getting Started

This chapter provides an introduction to using the touchscreen and optionalGen5 software to control the 800 TS. It also contains recommendations foroptimum performance.

External Components 28

Operate the Reader Using the Touchscreen 31

Operate the Reader Using Gen5 Software 38

Recommendations for Optimum Performance 41

28 | Chapter 3: Getting Started

External Components

1 Microplate carrier

2 Touchscreen

3 Power switch

4 USB flash drive port for USB flash drive

Note that the photo does not show the door.


External Components | 29

Rear ports on a reader without incubation capability

1 Power port

2 USB port

3 DO NOT USE! For BioTek Service only

4 USB port for printer

Rear ports on a reader with incubation capability

1 Power port

2 USB port

3 DO NOT USE! For BioTek Service only

4 USB port for printer



Optional Printer

Refer to the manual on CD that ships in the printer’s box for more details.

The 800 TS supports outputting the System Test report, the Absorbance Test Plate report,and measurement results including Raw OD, Delta OD, and blanked data to a thermalpaper printer.

Load the Printer Paper

1. Ensure the printer is turned on.

2. Press the gray tab on the right side of the printer to open the door.

3. Place a roll of paper into the indented space on the back of the door with the leadingedge of the paper coming from the top of the roll.

The printer prints on only one side of the paper. Ensure that the paper roll is installedso that the shiny side of the paper faces up. The printer will not print on the matte sideof the paper.

4. Unroll the paper just enough so that it clears the end of the door, then firmly close thedoor until it clicks into place. The printer advances the paper and cuts it.

Use the Printer

l To test that the printer is properly installed and communicating with the reader, inthe Main Menu, tap Instrument, then on the Option tab, tap Start to begin thePrinter Test. A short test result prints. If the results did not print, check that the powercable and USB cable are securely connected and run the test again.

l To print the results of a read (both a Quick Read and a defined protocol read), tapOutput in the top-right corner of the results screen displayed when the read finishes.

l To print System Test results, tap Print in the screen that appears when the systemtest finishes.

l To cleanly tear the paper from the printer, pull upward from left to right.

l To advance the paper, press Feed on the front of the printer.

l To turn off the printer, press and hold the ON/OFF button for at least three seconds.


Operate the Reader Using the Touchscreen | 31

Operate the Reader Using the Touchscreen

General Information

Do not use a sharp stylus or pencil on the touchscreen. Doing so can damage thetouchscreen's surface. You can use a stylus designed for capacitive touchscreens.

When you turn on the 800 TS, the touchscreen turns on automatically and opens to theMain Menu after the start-up system test and bulb warm-up. The length of the bulb warm-up process varies from three minutes for narrow beam and UV models, and 30 secondsotherwise. See Turn on the Reader in Chapter 2.

l To select a button or check box or to activate a tab, tap the item once.

l To return to the Main Menu from any other screen, tap Home in the top-left corner.

l For instructions on cleaning the touchscreen, see page 46.

l To preserve the life of the bulb, turn it off when not needed by tapping in theInstrument or Quick Menu. Note that if you turn off the bulb, the reader will gothrough the bulb warm-up process when you turn the bulb back on (three minutes fornarrow beam and UV models, and 30 seconds otherwise).

Main Menu



800TSI and 800TSUVI models: When the incubator is turned on, the reader’s internaltemperature is displayed at the top of the screen, as shown above. When the incubatoris off, dashes are displayed.

The left side of the Main Menu screen lists the assay protocols defined on the reader. Asshipped, the reader contains no protocols and the list is empty. Up to 40 uniquely namedprotocols can be programmed and stored. Tap Next to scroll through the list.On the right side of the Main Menu are the following options:

l Quick: Define and run a simple single- or dual-wavelength protocol. You can selectthe primary and secondary wavelength values and the plate type. If applicable to yourreader model, shake and/or incubate options are available.

l Protocol: Edit, create (and save), delete, and copy protocols. You can define theprotocol name; select the primary and secondary wavelength values, read speed, andplate type; and define blank well(s). If applicable to your reader mode, shake optionsare available.

The reader automatically performs delta OD and blank subtraction. To select thedataset(s) for print or export, tap Instrument and then the Output tab.

l Results: View and optionally print/export measurement data stored on the reader(for the 12 most recently run protocols).

l Instrument: Configure the reader, printer, and USB flash drive settings; define resultsoutput criteria; run an Absorbance Plate Test; and more.

Configure Your 800 TSAfter you install the reader, and before you use it to create and run protocols, perform thetasks in this section to define important instrument settings.

Set the Time and Date

When you turn on the 800 TS for the first time, set or confirm the date and time at yourlocation.



1. From theMain Menu, tap Instrument, the Config tab,then the Time button.

2. Tap the hour value, and use the keypad to enter thecorrect time for both the hour and minutes, then click OK.

Note: You can change just theminutes value: Tap theminutes value and enter the correct time.

3. Tap theDate button.

4. Tap month, day, or year, and use the keypad to set thecurrent date. Tap OK when done.

Verify or Change the Reader’s Filter Table




1. From theMain Menu, tap Instrument, the Config tab,and then Filter Table. Filter wheel locations 1 through 5are shown, with each location’s filter value (in nm) or“plug” for a blank filter.

2. Verify that the values match the contents of the filterwheel. See the Installation chapter for instructions foraccessing the filter wheel.

3. To change the setting for a filter wheel position, tap itsvalue and use the keypad to enter a wavelength value (innm), or select Plug. Tap OKwhen finished.

4. If you made any changes, tap Save in the Installed Filtersscreen. The filter values are now available for selection inprotocols and the absorbance test plate setup screen.

Define Regional Settings

1. From theMain Menu, tap Instrument, the Config tab,and then Regional Settings.

2. Tap to toggle between:

-Time format: AM/PM or 24 hour

-Date format: mm/dd/yyyy or dd/mm/yyyy

-Decimal symbol: period or comma (Note: This settingdoes not apply to the system test report content.)

-List separator: comma or semicolon (used in theexported report .csv file)

Define Output Formats for Measurement Data

If you want to send results to a printer connected to thereader:

1. From theMain Menu, tap Instrument, then tap theOutput tab.

2. Toggle the Printer button to Enabled, and select thedata to be included on the printout (you can select morethan one):



l Raw OD: The rawmeasurement value for each well.

l Delta OD: Applicable only when a secondary wavelength is selected. This is the calculatedvalue for each well of the primary wavelength measurement minus secondary wavelengthmeasurement.

l Blanked. The calculated value for each well, either RawODminus the average of the blankwells for single-wavelength reads or Delta ODminus the average of the blank wells for dualwavelength reads.

If you want to send results to a USB flash drive inserted in the reader, on the Output tab,toggle the USB Flash Drive button among the options.

l Report: Generates a CSV file containing themeasurement values (with RawOD/DeltaOD/blanked values, as applicable). This file can be opened in Excel or other spreadsheetsoftware.

l Gen5 Input: Generates a text file that can be opened in Gen5 using the Read from File option.This file contains only raw data, not delta ODs or blanks.

See page 92 for sample CSV and text files.

Define and Start a Quick Protocol

Note: This screenshot is from an instrumentwith incubation and shake capabilities.

1. From theMain Menu, tap Quick.

2. In the Quick Menu, set the Primary and, ifapplicable, Secondary wavelengths and plate type,then tap Start.

Note: You cannot define blank wells for a Quick read.

When the read is finished, the results are displayed.Tap Output to send results to the printer or USB flashdrive.

Create and Save a Protocol

The maximum number of uniquely named protocols that can be saved on thetouchscreen at the same time is 40.

Create a Protocol

1. From the home screen, tap Protocol, then tap Create.



2. Use the onscreen keyboard to enter a name for theprotocol, then tap OK.

Note: The <-- key circled in the figure is the backspacekey.

Note that the Options tab is not displayed on allinstruments.

3. Toggle through the values to set a Primarywavelength.

4. If applicable, toggle through the values to set aSecondary wavelength. Otherwise, leave as “---.”Note: The reader automatically performs Delta ODsubtraction.

5. Toggle to set a read speed: Normal, Rapid, orSweep. See the Throughput values associated witheach read speed type in the Specifications appendix.Note that Sweep is not available on the NB model.

6. Toggle to set the plate type: 6-, 12-, 24-, 48-, 96, or 384*-well microplates.

7. Note: Only full plate reads are supported on the touchscreen. To read a partial plate, you mustuse Gen5.

8. If applicable, tap the Blank tab to add blank wells.

a. In the Blank Well screen, use the keypad to enter which well should be blank, thentap OK.

b. Tap Add to enter more blank wells, up to 12.

9. If the reader is equipped with shake capability, tap Options and define a shake step, ifdesired. Set the shake’s duration using the keypad and intensity by toggling through theoptions.

10. Tap Save. The protocol now appears in the protocol list.

*Not all instruments support 384-well microplates.



Start a Protocol

For instruments equipped with incubation capability: To perform incubation duringmeasurement, either turn on incubation from the Incubate tab (Quick Menu) or tap thetemperature to the left of the Run Protocol (in a defined protocol).

The onboard storage for the touchscreen can accommodate data for up to 12microplate reads. The data is stored by date and time, not by filename. If 12 results arealready in onboard storage, the next read data saved will overwrite the oldest of thesaved results.

Note that only plate read results are saved. System Test and Absorbance Plate Testresults are not saved; they can only be exported to USB flash drive or printed.

1. From theMain Menu, tap the protocol youwant to run. The Run Protocol screen opens,displaying the protocol’s parameters.

2. Place themicroplate on the carrier, and tapStart. When the read is finished, the results aredisplayed. Tap Output to send results to theprinter or USB flash drive.

Change the Protocol Display Order

1. From the Main Menu, tap Instrument, then tap the Options tab.

2. In the Protocol display order field, select Alphabetically or Last accessed first.

Edit, Delete, or Copy a Protocol

Edit a Protocol

1. From the Main Menu, tap Protocol.

2. Tap the protocol that you want to modify, then tap Edit.

3. Make any desired changes, then tap Save.



Delete a Protocol

1. From the Main Menu, tap Protocol.

2. Tap the protocol you want to delete, then tap Delete.

Copy a Protocol

Copying an existing protocol and then editing it is a quick way to create a new protocol.

1. In the Main Menu, select the protocol you want to copy, then tap Copy.

2. You are prompted to enter a name for the copied protocol.

3. Make any desired changes, then tap Save.

View or Output Results Stored on the Reader

BioTek supplies a blank USB flash drive with the instrument if you choose to output thesystem test or assay results.

1. In the Main Menu, tap Results, then select the date/time of the read for which youwant to view or output results. The results are displayed on the touchscreen.

2. Tap Output. The results are printed and/or saved to the USB flash drive, dependingon the output format you selected (see page 34).

Operate the Reader Using Gen5 Software

Gen5 RC (Reader Control) software is supplied with the 800 TS. This edition supportsonly instrument control and data reporting/exporting. To perform kinetic reads, datareduction, and custom exports, a software upgrade is required; contact BioTekCustomer Care.

BioTek Gen5 software supports all 800 TS reader models. Use Gen5 to control the reader;perform data reduction and analysis on the measurement values; print or export results;and more. This section provides brief instructions for working with Gen5 to createprotocols and experiments and read plates. Refer to the Gen5 Help system for moreinformation.


Operate the Reader Using Gen5 Software | 39

Verify the Filter Table


1. From the Gen5 main screen, select System > Instrument Configuration, highlight the800 TS, and click View/Modify.

2. Click Setup, and then click the Absorbance tab.

3. Verify that the values match the contents of the filter wheel. See the Installationchapter for instructions for accessing the filter wheel.

4. To change the setting for a filter wheel position, enter the new value and click SendWavelengths.

5. Click Close when you are finished.

Protocols and ExperimentsIn Gen5, a protocol contains instructions for controlling the reader and (optionally)instructions for analyzing the data retrieved from the reader. At a minimum, a protocolmust specify the procedure for the assay you wish to run. After creating a protocol, createan experiment that references the protocol. You’ll run the experiment to read plates andanalyze the data.These instructions briefly describe how to create a protocol in Gen5. See the Gen5 Helpsystem for complete instructions.

1. In the Gen5 Task Manager, select Protocol > Create New.

2. Open the Procedure dialog. If prompted to select a reader, select the 800 TS and clickOK.

3. Select a Plate Type.

Gen5 stores measurements and other characteristics for individual plate types in adatabase. It is essential that you select (or define) the plate type to match the assayplate. Otherwise, results may be invalid. See the "Plate Type Database" topic in theGen5 help for instructions.

4. Add steps to the procedure for reading, shaking, or heating the plate and more. ClickValidate to verify that the reader supports the defined steps, and then click OK.



5. Optionally, perform any of these steps to analyze and report the results:

l Open the Plate Layout dialog and assign blanks, samples, controls, and/orstandards to the plate.

l Open the Data Reduction dialog to add data reduction steps. Categories includeTransformation, Well Analysis, Curve Analysis, and Qualitative Analysis.

l Create a report or export template via the Report/Export Builders.

6. Select File > Save and give the file an identifying name.

These instructions briefly describe how to create an experiment and then read a plate inGen5. See the Gen5 Help system for complete instructions.

1. In the Gen5 Task Manager, select Experiment > Create using an existing protocol.

2. Select the desired protocol and click OK.

3. Select a plate in the menu tree and click .

4. When the read is complete, measurement values appear in Gen5. Select the desireddata set from the Data list.

5. Select File > Save and give the file an identifying name.

Plate Shaking OptionsThe 800 TS supports linear plate shaking, with user-selected amplitude from 1 mm to 6mm, in 1 mm steps. Frequency ranges from about 18 Hz to about 6 Hz, depending on theselected amplitude. Shaking is controlled using Gen5 by adding a Shake step to a protocol'sprocedure.


Recommendations for Optimum Performance | 41

Gen5 Shake Step options

Recommendations for Optimum Performance

General

l Microplates should be clean and free from dust or bottom scratches. Use newmicroplates from sealed packages. Do not allow dust to settle on the surface of thesolution; use microplate covers or seals when not reading the plate. Filter solutions toremove particulates that could cause erroneous readings.

l Although the 800 TS supports standard flat, U-bottom, and V-bottom microplates, thereader achieves optimum performance with flat-bottomed wells. See Appendix A,Specifications for more information on the supported plates.

l Non-uniformity in the optical density of the well bottoms can cause loss of accuracy,especially with U- and V-bottom polyvinyl microplates. Check for this by reading anempty microplate. Dual wavelength readings can eliminate this problem, or bring thevariation in density readings to within acceptable limits for most measurements.

l Inaccuracy in pipetting has a large effect on measurements, especially if smallervolumes of liquid are used. For best results in most cases, use at least 100 µL per wellin a 96-well plate and 25 µL in a 384-well plate.

l Pipetting solution into 384-well plates often traps air bubbles in the wells, which mayresult in inaccurate readings. A dual-wavelength reading method usually eliminatesthese inaccuracies. For best results, however, remove the air bubbles by degassingthe plate in a vacuum chamber or spinning the plate in a centrifuge before reading.

l The inclination of the meniscus can cause loss of accuracy in some solutions,especially with small volumes. Shake the microplate before reading to help bring itwithin acceptable limits. Use Tween 20, if possible (or some other wetting agent) tonormalize the meniscus for absorbance measurements. Some solutions develop



menisci over a period of several minutes. This effect varies with the brand ofmicroplate and the solution composition. As the center of the meniscus drops andshortens the light path, the density readings change. The meniscus shape will stabilizeover time.

l It is the user’s responsibility to understand the volumetric limits of the plate type inuse as it applies to the assay being run.

l Use of liquids with concentrations of acids, corrosives, or solvents of 3% and greatercan begin attacking the materials inside the instrument's chamber. Running multipleplates with concentrations < 3% in long kinetic experiments may also have adestructive effect. If the experiment is incubated, it will accelerate the deteriorationof chamber components. When in doubt about the use of acids, corrosives, orsolvents, please contact [email protected].

Using 384-Well MicroplatesWhen using a 384-well microplate (NB models only), you can use the Gen5 Auto Mapfeature to ensure you are using an accurate plate map for your reads. See the Gen5 Helpfor more information.

Incubation and Partial PlatesPartial plate reads can be defined only through Gen5, not using the touchscreen. Whenperforming a partial plate read that includes an incubation step, the followingrecommendations can reduce the effects of evaporation of your samples:

l Use microplate lids.

l Fill unused wells with fluid.

l Cluster your sample wells rather than spacing them throughout the plate.

l Place your sample wells in the center of the plate. This placement may lead to lessevaporation than if you place the samples in wells on the edge of the plate.


Chapter 4

Maintenance

This chapter provides instructions for cleaning and decontaminating the 800TS.

Maintenance Overview 44

Warnings and Precautions 45

Clean Exposed Surfaces 45

Clean the Touchscreen 46

Decontamination 47

Filter Storage and Handling 49

Replacing and Aligning the Bulb 49

44 | Chapter 4: Maintenance

Maintenance OverviewA general maintenance regimen for all 800 TS models includes periodically cleaning allexposed surfaces and decontaminating the instrument before storage or shipment. Thischapter includes instructions for the following:

l Routine cleaning procedure, page 45

l Cleaning the touch screen, page 46

l Decontamination, page 47

l Filter storage and handling, page 49

l Replacing and aligning the bulb, page 49

Required Materials

l Mild detergent

l Deionized or distilled water

l Clean, lint-free cotton cloths

l Sodium hypochlorite (NaClO, or bleach) (decontamination only)

l Safety glasses

l Surgical mask

l Protective gloves

l Lab coat

l Biohazard trash bags

l 125-mL beakers

l Cotton swabs or paper towels


Warnings and Precautions | 45

Warnings and PrecautionsRead the following before performing any maintenance procedures:

Warning! Internal Voltage. Turn off and unplug the instrument for all maintenanceand repair operations.

Important! Do not immerse the instrument, spray it with liquid, or use a dripping-wet cloth on it. Do not allow water or other cleaning solution to run into theinterior of the instrument. If this happens, contact the BioTek Service Department.

Do not soak the touchscreen! This will cause damage. Moisten a clean cloth withdeionized or distilled water and wipe the touchscreen. Dry immediately with aclean, dry cloth.

Important! Do not apply lubricants to the microplate carrier or carrier track.Lubricant attracts dust and other particles, which may obstruct the carrier path andcause errors.

Warning!Wear protective gloves when handling contaminated instruments. Glovedhands should be considered contaminated at all times; keep gloved hands awayfrom eyes, mouth, nose, and ears.

Warning!Mucous membranes are considered prime entry routes for infectiousagents. Wear eye protection and a surgical mask when there is a possibility ofaerosol contamination. Intact skin is generally considered an effective barrieragainst infectious organisms; however, small abrasions and cuts may not always bevisible. Wear protective gloves when handling contaminated instruments.

Clean Exposed Surfaces

This procedure is for the housing of the 800 TS instrument. See "Clean the TouchScreen" on page 46 for the cleaning procedure for the touch screen.

A regular cleaning regimen is recommended to keep the instrument free from dust andparticulates that can cause erroneous readings. Exposed surfaces may be cleaned (notdecontaminated) with a cloth moistened (not soaked) with water or water and a milddetergent.



1. Turn off and unplug the instrument from the power supply.

2. Wet a clean cotton cloth with water, or with water and mild detergent, then thoroughlywring out the cloth so that liquid does not drip from it.

3. Wipe the plate carrier, the inside of the plate carrier door (if equipped), and all exposedsurfaces of the instrument.

4. If detergent was used, wipe all surfaces with a cloth moistened with water.

5. Use a clean, dry, lint-free cloth to dry all wet surfaces.

If liquid is spilled inside the reader, call BioTek TAC for cleanup instructions.

Clean the Touchscreen

Important! Never spray solutions directly on the touchscreen.

MaterialsUse the following products to safely clean the touchscreen:


l Dish soap or other mild cleaner

l Lintfree disposable towels

Avoid the following cleaning products:

l Strong solvents, such as alcohol, acetone, ammonium chloride, methylenechloride, and hydrocarbons, which can permanently damage the surface ofthe touchscreen.

l Fibrous materials, such as paper towels, which can scratch thetouchscreen. Over time, dirt particles and cleaning agents can get trappedin the scratches.

Procedure

Important! Never spray solutions directly on the touchscreen.


Decontamination | 47

1. Turn off and unplug the instrument.

2. Moisten a clean, lint-free disposable cloth with water, or with water and mild detergent,then thoroughly wring it out so that liquid does not drip from it. Do not soak the cloth.

3. Wipe the touch screen gently with the moist cloth.

4. If detergent was used, wipe the touchscreen with a cloth moistened with water.

5. Dry the screen gently using another cloth.

DecontaminationAny laboratory instrument that has been used for research or clinical analysis is considereda biohazard and requires decontamination prior to handling.Decontamination minimizes the risk to all who come into contact with the instrumentduring shipping, handling, and servicing. Decontamination is required by the U.S.Department of Transportation regulations.Persons performing the decontamination process must be familiar with the basic setup andoperation of the instrument.

Turn off and unplug the instrument for the decontamination procedure.

BioTek Instruments, Inc., recommends the use of the following decontaminationsolutions and methods based on our knowledge of the instrument andrecommendations of the Centers for Disease Control and Prevention (CDC). NeitherBioTek nor the CDC assumes any liability for the adequacy of these solutions andmethods. Each laboratory must ensure that decontamination procedures areadequate for the biohazard(s) they handle.

Wear prophylactic gloves when handling contaminated instruments. Gloved handsshould be considered contaminated at all times; keep gloved hands away fromeyes, mouth, and nose. Eating and drinking while decontaminating instruments isnot advised.

Mucous membranes are considered prime entry routes for infectious agents. Weareye protection and a surgical mask when there is a possibility of aerosolcontamination. Intact skin is generally considered an effective barrier againstinfectious organisms; however, small abrasions and cuts may not always be visible.Wear protective gloves when performing the decontamination procedure.



Decontaminating the Reader Housing

Required Materials

l Sodium hypochlorite (NaClO, or bleach)

l 70% isopropyl alcohol (as an alternative to bleach)


l Safety glasses

l Surgical mask

l Protective gloves

l Lab coat

l Biohazard trash bags

l 125-mL beakers

l Clean, lint-free cotton cloths

Procedure

1. Turn off and unplug the reader from the power supply.

2. Prepare an aqueous solution of 0.5% sodium hypochlorite (NaClO, or bleach). If theeffects of bleach are a concern, 70% isopropyl alcohol may be used.

Check the percent NaClO of the bleach you are using. Commercial bleach is typically10.0% NaClO; prepare a 1:20 dilution. Household bleach is typically 5.0% NaClO;prepare a 1:10 dilution.

3. Moisten a clean, lint-free cloth with the bleach solution, then thoroughly wring it out sothat liquid does not drip from it. Do not soak the cloth.

4. Wipe the plate carrier and all exposed surfaces of the instrument, except the touchscreen (if equipped).

5. Allow the instrument to dry for 20 minutes for thorough decontamination by the bleach.

6. Moisten a cloth with deionized or distilled water and wipe all surfaces of the instrumentthat have been cleaned with the bleach solution.


Filter Storage and Handling | 49

7. Use a clean, dry lint-free cloth to dry all wet surfaces.

8. Discard the used gloves and cloths, using a biohazard trash bag and an approvedbiohazard container.

Decontaminating the TouchscreenWhen decontaminating the 800 TS, as described on page 47, do not spray the bleachsolution on the touchscreen. Avoid fibrous materials that can scratch the surface. Do notuse a stronger bleach solution or cleaning solvent than recommended.

Filter Storage and HandlingTo properly store interference filters during extended periods of non-use, package thefilters in a light-tight envelope or container, away from high humidity. This will ensure thelongest life for the filters. When handling the filters, keep the surfaces clean fromfingerprints and debris by simply wiping with a lens tissue or other lint-free cloth.

When changing or replacing filters, it is critical that the filters be placed in thefilter wheel in the correct orientation, with the light-direction arrow pointingdownward. Also, the reader or Gen5 software filter table must exactly match thecontents of the filter wheel.

Replacing and Aligning the BulbThe tungsten or halogen bulb used on the various models of the 800 TS is user-replaceable.The intensity of each type of bulb will slowly drop over time until the start-up self-checkdetects a low signal level and the instrument displays an error code. The bulb should bereplaced and aligned at this time.

l For the 800TS and 800TSI models, order replacement bulb kit PN 7330513.

l For the 800TSUV, 800TSSUVI, and 800TSNB models, order replacement bulb kitPN 7330516.

The kits contain complete instructions for replacing and aligning the bulb.

Warning! The bulb will be hot immediately after the reader has been shut down.Allow the bulb to cool for at least 10 minutes before attempting to replace it.

Warning! The alignment procedure requires you to observe the light path whilethe bulb is turned on. To prevent possible vision impairment, avoid lookingdirectly at the bulb while it is on.


Chapter 5

Instrument Qualification Process

This chapter describes the tests that BioTek Instruments, Inc., has developedfor complete qualification of all models of the 800 TS. This chapterintroduces the various test methods, describes the materials and protocolparameters used to execute the tests, explains how to analyze test results,and provides troubleshooting tips in the event of a failure. InstrumentQualification Procedures, starting on page 61, contains the actual step-by-step test procedures.

Instrument System Test 52

Absorbance Testing 52

52 | Chapter 5: Instrument Qualification Process

Instrument System TestEach time the 800 TS is turned on, it automatically performs a series of tests on thereader’s motors, lamp, and optical systems. If all tests pass, the microplate carrier movesto its forward position and the Main Menu appears on the touchscreen.If any test results do not meet the internally coded Failure Mode Effects Analysis (FMEA)criteria established by BioTek, the reader beeps repeatedly and an error message appearson the touchscreen. If this occurs, tap OK on the touchscreen to stop the beeping. Ifnecessary, initiate another system test using Gen5 or the touchscreen to try to retrieve anerror code from the reader.Refer to Error Codes, starting on page 81, for information on error codes andtroubleshooting tips.Refer to Sample Reports, starting on page 89, to see a sample System Test Report for 800TS.

Absorbance TestingBioTek developed a series of tests for the absorbance system using a combination of solidstate Absorbance Test Plates and liquid plates. The test plates and the materials used forcreating the liquid plates are available for purchase from BioTek.To qualify the absorbance system for the 800 TS, you should perform:

l Absorbance Liquid Test 1 and Absorbance Plate Test (using BTI #7260522) or

l Absorbance Liquid Test 2

Optionally, to qualify operation in the UV range, you should also perform:

l Absorbance Liquid Test 3 or Absorbance Plate Test at 340 nm (using BTI #7260551)

BioTek Absorbance Test PlatesAbsorbance Test Plate PN 7260522 uses NIST-traceable neutral density filters to confirmabsorbance specifications in the visible range (400–800 nm). This test plate also containsprecision-machined holes to verify mechanical alignment. Absorbance Test Plate PN7260551 uses NIST-traceable neutral density filters to confirm absorbance specifications inthe UV range (340 nm).Every test plate comes with a Test Plate Calibration Certificate, containing a table withAbsorbance OD Standards for each filter at each wavelength supported by the plate.Before the Absorbance Plate Test can be performed, the OD Standard values must bedefined on the reader via the touchscreen or entered into Gen5. Enter and save thesevalues once initially, and then update them annually when the test plate is recertified byBioTek.


Absorbance Testing | 53

Check the calibration due date on the test plate's label. If the test plate is overdue forrecalibration, contact BioTek to schedule service.

Test Method

The Absorbance Plate Test is conducted using the 800 TS touchscreen or Gen5 software(System > Diagnostics > Test Plates) to confirm mechanical alignment and optical densityaccuracy, linearity, and repeatability. When complete, a results report displays Pass or Failfor each individual test.

l Alignment: The test plate has precisely machined holes to confirm mechanicalalignment. The amount of light that shines through these holes is an indication ofwhether the microplate carrier is properly aligned with the absorbance optical path.A reading of more than 0.015 OD for any of the designated alignment holes indicatesthat the light is being “clipped” and the reader may be out of alignment.

l Accuracy: The test plate contains NIST-traceable neutral-density glass filters of knownOD values at one or more wavelengths. Actual measurements are compared againstthe expected values provided in the test plate’s data sheet. Since there are severalfilters with differing OD values, the accuracy across a range of ODs can beestablished. Once it is proven that the reader is accurate at these OD values, thereader is also considered to be linear. To further verify this, you can perform a linearregression analysis on the test plate OD values in a program such as Microsoft Excel;an R2 value of at least 0.9900 is expected.

l Repeatability: This test ensures the instrument meets its repeatability specificationby conducting repeated reads of each neutral-density filter on the test plate andcomparing the results.

Sample Test Report

Refer to Sample Reports, starting on page 89, to see a sample Absorbance Plate TestReport for 800 TS.

Troubleshooting

If a test fails, try the troubleshooting tips below. If the test continues to fail, contact BioTekTAC.

Important! Do not remove filters from the Absorbance Test Plate. Do not use alcohol orother cleaning agents, and do not touch the filters with your bare fingers.

If a higher-OD well reports "#N/A" for Min/Max Limit and Result, themeasured OD isbeyond the specified range for Accuracy or Repeatability used with this test, andtherefore no pass/fail determination is made. It does not indicate a test failure.



Alignment Test

l Ensure that the test plate is properly seated in the microplate carrier.

l Check the four alignment holes (B2, B12, G1, G11) to ensure they are clear of debris.

l Check the microplate carrier to ensure it is clear of debris.

Accuracy Test

l Check the neutral-density filters to ensure they are clean (positions C1, D4, E2, F5, G3,H6). If needed, clean the filters with lens paper. Do not remove any filters, and do notuse alcohol or other cleaning agents.

l Verify that the wavelength/expected OD values entered for the plate on the reader orin Gen5 match the information provided on the test plate's data sheet.

Repeatability Test

l Check the neutral-density filters to ensure there is no debris that may have shiftedbetween readings and caused changes.

l Check the microplate carrier to ensure it is clear of debris.

Absorbance Liquid TestsBioTek Instruments, Inc., has developed a series of liquid test procedures for testing yourreader's absorbance system.

Test Methods

Absorbance Liquid Test 1 confirms repeatability and alignment of the reader when asolution is used in the microplate. If these tests pass, then the lens placement and opticalsystem cleanliness are proven. For the Repeatability portion of this test, two columnscontaining a color-absorbing solution are read five times at 405 nm. For each well, an"allowed deviation" is determined based on its Mean OD and the reader's repeatabilityspecification. Each well's Standard Deviation must be less than its Allowed Deviation topass. To confirm the reader’s mechanical alignment, the plate is rotated 180 degrees inthe carrier (e.g., A1 is now in the H12 position) and the same two columns are read. Theinitial and new OD readings are compared, using the reader's accuracy specification. If thetwo readings in the same well do not meet specification, the reader may be out ofalignment.If an Absorbance Test Plate is not available, Absorbance Liquid Test 2 may be conductedto test the instrument's alignment, repeatability, and accuracy by preparing a series ofsolutions of varying OD values as described on page 71.



Absorbance Liquid Test 3 is an optional test offered for those sites that must have proof oflinearity at 340 nm. (Alternatively, the BioTek 340 nm Absorbance Test Plate may be used;see page 52.) This test is optional since the 800 TS has good “front end” linearitythroughout the specified wavelength range. While the absolute values of the OD cannot bedetermined by this test, the results will indicate if there is adequate repeatable absorbanceand a linear slope. This method is dependent upon proper dye dilution and a skilledpipetting technique. It is expected that the first dilution (mid-level solution) will have anabsorbance value near 75% of that of the stock (high-level) solution, and that the seconddilution (low-level solution) will have an absorbance value near 50% of that of the stocksolution.

Gen5 Protocol Parameters

If using Gen5 to conduct the absorbance liquid tests, follow these instructions. Theinformation in this section represents the recommended reading parameters for thereferenced Gen5 protocol(s). It is possible that your tests will require modifications to someof these parameters, such as the Plate Type.

The Plate Type setting in each Gen5 protocol should match the actual 96-well plate inuse.

800 TS Abs Test 1.prt

Parameter Setting

Plate Type 96WELL PLATE

Two Read Steps

Kinetic loop (one per Read step)Set a Run Time/Interval combination to read theplate five times with minimal delay

Detection Method Absorbance

Read Type Endpoint

Read wellsFirst Read step: A1..H2Second Read step: A11..H12

Wavelength 405 nm

Read Speed Normal

Delay after platemovement 100msec

Plate Out,In step between loops Text "rotate the plate 180 degrees"




Parameter Setting


Two Read Steps



Read Type Endpoint

Step labelsFirst Read step: "Normal"Second Read step: "Turnaround"

Read wells Full plate

Wavelength 2 (450 nm, 630 nm)

Read Speed Normal


Data ReductionDefine two Delta OD transformations (450 – 630nm), one per Read data set


Parameter Setting


Shake Step (if supported) Linear, 4minutes, default frequency

Two Read Steps



Read Type Endpoint

Read wells A1..H6

Wavelength 340 nm

Read Speed Normal




Results Analysis

The Absorbance Liquid Test procedures begin on page 68. All tests are conducted using theNormal read speed.

Absorbance Liquid Test 1

Accuracy Specification:± 1.0% ± 0.010 OD from 0.000 to 2.000 ODRepeatability Specification:± 0.5% ± 0.005 OD from 0.000 to 2.000 OD

1. The plate is read five times in the “Normal” position at 405 nm. Calculate the Mean ODand Standard Deviation of those five reads for each well in columns 1 and 2.

2. For each well in columns 1 and 2, calculate the Allowed Deviation using theRepeatability specification for a 96-well plate (Mean OD x 0.05 + 0.005). For each well,its Standard Deviation should be less than its Allowed Deviation.Example: Five readings in well A1 of 0.802, 0.802, 0.799, 0.798, and 0.801 result in aMean of 0.8004 and a Standard Deviation of 0.0018. The Mean multiplied by 0.5%(0.8004 * 0.005) equals 0.004, and when added to 0.005 equals 0.009; this is the AllowedDeviation for well A1. Since the Standard Deviation for well A1 is less than this value,the well meets the test criteria.

3. The plate is read five times in the “Turnaround” position at 405 nm. Calculate the MeanOD of those five reads for each well in columns 11 and 12.

4. Perform a mathematical comparison of the Mean values for each well in its Normal andTurnaround positions (that is, compare A1 to H12, A2 to H11, B1 to G12,… H2 to A11). Topass the test, the differences in the compared Mean values must be within the Accuracyspecification for a 96-well microplate.Example: If the Mean value for well A1 in the Normal position is 1.902 with a specifiedaccuracy of ±1.0% ±0.010 OD, then the expected range for the Mean of the well in itsTurnaround (H12) position is 1.873 to 1.931 OD. 1.902 x 0.010 + 0.010 = 0.029; 1.902 -0.029 = 1.873; 1.902 + 0.029 = 1.931.


Accuracy Specification:+/-1.0% +/-0.010 OD from 0.000 to 2.000 ODRepeatability Specification:+/-0.5% +/-0.005 OD from 0.000 to 2.000 OD



1. The plate is read five times at 450/630 nm ("Normal" position), resulting in five sets ofDelta OD data. Calculate results for Linearity:

l Calculate the mean absorbance for each well, and average the means for eachconcentration.

l Perform a regression analysis on the data to determine if there is adequatelinearity. Since it is somewhat difficult to achieve high pipetting accuracy whenconducting linear dilutions, an R2 value of at least 0.9900 is considered adequate.

2. Calculate the results for Repeatability:

l Calculate the Mean and Standard Deviation for the five readings taken at eachconcentration. Only one row of data needs to be analyzed.

l For each Mean, calculate the Allowed Deviation using the Repeatabilityspecification for a 96-well plate of ±0.5% ±0.005 OD.

l The Standard Deviation for each set of readings should be less than the AllowedDeviation.Example: Readings of 1.950, 1.948, 1.955, 1.952, and 1.950 will result in a Mean of1.951, and a Standard Deviation of 0.0026. The Mean (1.951) multiplied by 0.5%(1.951 x 0.005) = 0.009755, which, when added to the 0.005 (0.009755 + 0.005) =0.0148 OD, which is the Allowed Deviation. Since the Standard Deviation is lessthan this value, the reader meets the test criteria.

3. After gathering data for the Linearity Test, the plate is read five more times with the A1well in the H12 position ("Turnaround" position). This results in values for the fourcorner wells that can be used to assess alignment. Calculate results for the AlignmentTest:

l Calculate the means of the wells A1 and H1 in the Normal plate position (datafrom Linearity Test) and in the Turnaround position.

l Compare the mean reading for well A1 to its mean reading when in the H12position. Next, compare the mean values for the H1 well to the same well in theA12 position. The difference in the values for any two corresponding wells shouldbe within the Accuracy specification for 96-well plates. If the four corner wells arewithin the accuracy range, the reader is in alignment.Example: If the mean of well A1 in the normal position is 1.902, where thespecified accuracy is ±1.0% ±0.010 OD, then the expected range for the mean ofthe same well in the H12 position is 1.873 to 1.931 OD. (1.902 x 1.0% = 0.019 +0.010 = 0.029, which is added to and subtracted from 1.902 for the range.)




Repeatability Specification:+/-1.5% +/-0.005 OD from 0.000 to 2.000 OD @ 340nm

1. The plate is read five times at 340 nm. For each well, calculate the Mean OD andStandard Deviation of the five readings.

2. For each Mean calculated in step 1, calculate the Allowed Deviation using theRepeatability specification for a 96-well plate (Mean OD x 0.015 + 0.005). For each well,its Standard Deviation should be less than its Allowed Deviation.Example: Five readings in well A1 of 0.802, 0.802, 0.799, 0.798, and 0.801 result in aMean of 0.8004 and a Standard Deviation of 0.0018. The Mean multiplied by 1.5%(0.8004 * 0.015) equals 0.012, and when added to 0.005 equals 0.017; this is the AllowedDeviation for well A1. Since the Standard Deviation for well A1 is less than this value,the well meets the test criteria.

3. Calculate results for Linearity:

l For each of the three test solutions, calculate the average Mean OD for the wellscontaining that solution (mean of wells A1 to H2, A3 to H4, and A5 to H6).

l Perform a regression analysis on the data to determine if there is adequatelinearity. The three average Mean OD values are the “Y” values. The solutionconcentrations are the “X” values (1.00, 0.75, 0.50). Since it is somewhat difficultto achieve high pipetting accuracy when conducting linear dilutions, an R2 value ofat least 0.9900 is considered adequate.

Troubleshooting

If an absorbance liquid test fails, try the following. If a test continues to fail, contact BioTekTAC.

l Check the microwells and plate carrier for debris that may have shifted and causedchanges.

l Ensure the microplate is properly seated in the carrier.

l As applicable, confirm that the plate was properly oriented in the "Normal" and"Turnaround" positions.

l Liquid Test 1 can fail due to the meniscus effect, which can cause readings todecrease over time. If you suspect this may be the case, include a shake step betweenthe read steps in the protocol.


Chapter 5

Instrument Qualification Procedures

This chapter contains the step-by-step procedures for verifying that the 800TS and its various subsystems are performing to specification. InstrumentQualification Process, starting on page 51, introduces the various testmethods, describes the materials and relevant protocol parameters used toexecute the tests, explains how to analyze test results, and providestroubleshooting tips in the event of a failure.

Overview 62

IQ/OQ/PQ Description 62

Recommended Qualification Schedule 64

System Test 64

Absorbance Plate Test 66

Absorbance Liquid Tests 68

62 | Chapter 6: Instrument Qualification Procedures

OverviewThis chapter contains BioTek Instrument's recommended qualification procedures for all800 TS models.Every 800 TS reader is fully tested at BioTek prior to shipment and should operate properlyupon initial setup. If you suspect that a problem occurred during shipment, if you havereceived the equipment after returning it to the factory for service, and/or if regulatoryrequirements dictate that you qualify the equipment on a routine basis, perform theprocedures outlined in this chapter.See the Recommended Qualification Schedule on page 64 to determine whichqualification tests shall be conducted for your and to meet your site's regulatoryrequirements.

A Product Qualification Package (PN 1560516) for the 800 TS is available for purchase.The package contains complete procedures, Gen5 protocols, checklists, and logbooksfor performing Installation Qualification, Operational Qualification, PerformanceQualification, and Preventive Maintenance. Contact your local BioTek dealer for moreinformation.

IQ/OQ/PQ DescriptionInstallation Qualification confirms that the reader and its components have been suppliedas ordered and ensures that they are assembled and configured properly for your labenvironment.

l The recommended IQ procedure consists of setting up the instrument and itscomponents as described in Chapter 2, Installation, and performing the System Test.

l The IQ procedure should be performed initially (before the reader is used for the firsttime).

l The successful completion of the IQ procedure verifies that the instrument is installedcorrectly. The Operational Qualification procedure should be performed immediatelyfollowing the successful IQ.

Operational Qualification confirms that the equipment operates according tospecification initially and over time.

l The recommended OQ procedure consists of performing the system test, absorbanceplate test, and a series of liquid tests.

l The OQ procedure should be performed initially (before first use) and then routinely;the recommended interval is annually. It should also be performed after any majorrepair or upgrade to the hardware or software.


IQ/OQ/PQ Description | 63

l Although out-of-tolerance failures will be detected by the OQ tests, results should becompared with those from the routine Performance Qualification tests and previousOQ tests to monitor for trends.

l The successful completion of the OQ procedure, in combination with results that arecomparable to previous PQ and OQ tests, confirms that the equipment is operatingaccording to specification initially and over time.

Performance Qualification confirms that the reader consistently meets the requirementsof the tests performed at your laboratory.

l The recommended PQ procedure consists of performing the system test, absorbanceplate test, and a series of liquid tests.

l Your facility’s operating policies may also require that you routinely perform anactual assay, to confirm that the reader will consistently give adequate results for theassays to be run on it.

l These tests should be performed routinely; the recommended interval is monthly orquarterly, depending on the test. This frequency may be adjusted depending on thetrends observed over time.

l The successful completion of the PQ procedure confirms that the equipment isperforming consistently under normal operating conditions.



Recommended Qualification ScheduleThis schedule defines BioTek-recommended intervals for qualification for an instrumentused for one shift seven days a week.

The risk and performance factors associated with your assays may require that theOperational and Performance Qualification procedures be performed more or lessfrequently than shown here.

Tasks/Tests

IQ OQ PQ

InitiallyInitially/Annually

Monthly Quarterly

All models:

Unpacking, installation, and setup ü

System Test ü ü ü

Absorbance Plate Test ü ü

Absorbance Liquid Test 1 or Liquid Test2*

ü ü

(Optional, for UV models only)Absorbance Liquid Test 3 or 340 nmAbsorbance Plate Test (usingBTI #7260551)

ü ü

* If you have Absorbance Test Plate PN 7260522, perform Liquid Test 1. Otherwise,perform Liquid Test 2.

System Test

Instrument System Test, starting on page 20, describes this test and explains where tofind information on error codes and troubleshooting tips, as well as sample test reportsfor 800 TS.

SetupIf your assays use incubation, we recommend enabling temperature control for at least37°C and allowing the incubator to reach its set point before running the system test. Toaccess this feature:


System Test | 65

l On the touchscreen:

1. Tap the temperature display. Note that until Temperature Control is enabled,three dashes are displayed.

2. If necessary, turn on Temperature Control and enter a setpoint of at least37°C, then tap Home to return to the Main Menu.

l In Gen5, select System > Instrument Control, and click the Pre-Heating tab.

Test Procedure Using the Touchscreen

1. When the temperature display reaches the defined setpoint, tap Instrument, then tapOptions.

2. Under System test, tap Start.

3. When the test finishes, tap USB Report to save the test results to a USB flash drive,Print to print the test results, or Exit to close the screen.

Test Procedure Using Gen5

1. From the Gen5 main screen, select System > Diagnostics > Run System Test.

If the test fails during execution, a message box appears in the Gen5. Close the box; thetest report contains the error code that was generated by the failure.

2. When the test is complete, a dialog appears, requesting additional information. Enterany required information and then click OK.

3. The results report appears. it shows either “SYSTEM TEST PASS” or “SYSTEM TEST FAIL*** ERROR (error code) DETECTED.”

If the test failed, look up the error code in Appendix B, Error Codes, starting onpage 81, to determine its cause. If the cause is something you can fix, turn off thereader, fix the problem, and then turn the reader back on and retry the test. Ifthe test continues to fail, or if the cause is not something you can fix, contactBioTek TAC.

4. If required, print, sign, and date the report, and store it with your test documentation.

5. If applicable, turn off the incubator.



Absorbance Plate Test

BioTek Absorbance Test Plate, starting on page 52, describes the test methods andprovides troubleshooting tips in the event of test failure.

RequirementTo perform this test, you will need:

l Absorbance Test Plate, BTI #7260522

l (Optional) 340 nm Absorbance Test Plate, BTI #7260551

l (Optional) 340 nm filter installed in the instrument

l Current Absorbance Test Plate Calibration Certificate(s)

SetupBefore an Absorbance Test Plate can be used for qualification, you must enter informationfrom its Calibration Certificate into the reader or Gen5. Perform these steps initially, andthen repeat them annually after the test plate is recertified by BioTek.


1. Obtain the current Test Plate Calibration Certificate.

2. From the Main Menu, tap Instrument, the tap Test Plate.

3. Tap Test Plate Certificate and enter the plate’s serial number and Next Calibration Duedate, then tap Back to return to the Test Plate tab.

4. The wavelength selection buttons reflect the filters installed in the reader. Tap eachbutton, and enter the OD Standard values from the Calibration Certificate into the grid.Make sure you enter the correct value for each well/wavelength combination.

Using Gen5

1. Obtain the current Test Plate Calibration Certificate.

2. Start Gen5, and select System > Diagnostics > Test Plates > Add/Modify Plates.

3. Click Add. The Absorbance Test Plate dialog appears.


Absorbance Plate Test | 67

4. Select the appropriate Plate Type, and then enter the plate's serial number.

5. Enter the Last Certification and Next Certification dates from the calibration label onthe Test Plate.

6. If the wavelength values in the top row of the grid in Gen5 are appropriate for yourtests, enter the OD Standard values from the Calibration Certificate into the grid. Makesure you enter the correct value for each well/wavelength combination.

If you need to change the wavelength values, clickWavelength List. Add, change, ordelete the values as needed and click OK.

7. Review all of the values that you entered. When finished, click OK to save theinformation.

Test Procedure


1. Place the Absorbance Test Plate on the microplate carrier, with well A1 in the properlocation.

2. From the Main Menu, tap Instrument and then Test Plate.

3. Tap a Wavelength Selection button, then tap Start.

4. When the test is complete, choose an Output option (Print or USB Report), or tap Exitto return to the Main Menu.

Using Gen5

1. From the Gen5 main screen, click System > Diagnostics > Test Plates > Run. Ifprompted, select the desired Test Plate and click OK.

2. When the Absorbance Test Plate Options dialog appears, enter any requiredinformation.

3. Highlight the wavelength(s) to be included in this test.

You need to select only those wavelengths most appropriate for your use of the reader.



4. (Optional) Enter a comment.

5. Click Start Test.

6. Place the Absorbance Test Plate on the microplate carrier, with well A1 in the properlocation.

7. Click OK to run the test.

8. When the test is complete, the results report appears. Scroll through the report; everyresult should show “PASS”.

l Troubleshooting tips are provided on page 53.

l Test descriptions are provided on page 53.

Absorbance Liquid Tests

Absorbance Liquid Tests, starting on page 54, describes the test methods, lists theGen5 protocol parameters, explains how to analyze the test results, and providestroubleshooting tips in the event of test failure.


The tests in this section require specific microplates, solutions, and filters. Yourlaboratory may require a deviation from some of these tests. For example, you maywish to use a different plate or test solution. If deviation from the tests as presentedin this section is required, perform the following steps the first time each test is run:• Perform the tests exactly as described here.• Rerun the tests using your particular plates, solutions, and so on.• If the results are comparable, then the results from these tests will be yourbaseline for future tests. Document your new test procedure, and save all testresults.

Materials

Manufacturer part numbers are subject to change.


Absorbance Liquid Tests | 69

l New 96-well, clear, flat-bottom microplate (Corning Costar #3590 recommended)

l Stock Solution A or B, which may be formulated by diluting a dye solution availablefrom BioTek (A) or from the materials listed below (B).

l If using Gen5, the protocol 800 TS Abs Test 1.prt described on page 55

Solution A

l BioTek QC Check Solution No. 1 (PN 7120779, 25 mL; or 7120782, 125 mL)

l Deionized water

l 5-mL Class A volumetric pipette

l 100-mL volumetric flask

1. Pipette a 5-mL aliquot of BioTek QC Check Solution No. 1 into a 100-mL volumetric flask.

2. Add 95 mL of DI water; cap and shake well. The solution should measure approximately2.000 OD when using 200 µL in a flat-bottom microwell.

Solution B

l Deionized water

l FD&C Yellow No. 5 dye powder (typically 90% pure)

l Tween 20 (polyoxyethylene (20) sorbitan monolaurate) or BioTek wetting agent, PN7773002 (a 10% Tween solution)

l Precision balance with capacity of 100 g minimum and readability of 0.001 g

l 1-liter volumetric flask

l Weigh boat

1. Weigh out 0.092 gram of FD&C No. 5 yellow dye powder into a weigh boat.

2. Rinse the contents into a 1-liter volumetric flask.

3. Add 0.5 mL of Tween 20, or 5 mL of BioTek’s wetting agent.

4. Make up to 1 liter with DI water; cap and shake well.



Test Procedure

Be sure to use a new microplate. Debris, fingerprints, or scratches may causevariations in readings.

1. Using freshly prepared stock solution (Solution A or B), prepare a 1:2 dilution usingdeionized water (one part stock, one part deionized water; the resulting solution is a 1:2dilution). The concentrated stock solution should have an optical density ofapproximately 2.000 OD or lower.

2. Pipette 200 µL of the stock solution into column 1.

3. Pipette 200 µL of the diluted solution into column 2.

After pipetting the diluted test solution into the microplate and before readingthe plate, we strongly recommend shaking the plate for four minutes. This willallow any air bubbles in the solution to settle and the meniscus to stabilize.Alternatively, wait 20 minutes after pipetting the test solution before reading theplate.

If using the touchscreen:

4. Read the microplate five times at 405 nm using the Normal Read Speed. Whenprompted, rotate the plate 180 degrees and continue.

5. Print the five sets of raw data or send it to the USB flash drive for use in anotherprogram.

6. Perform the Results Analysis calculations described in Chapter 5.

If using Gen5:

4. Create a Gen5 experiment based on the 800 TS Abs Test 1 protocol and read the plate.When prompted, rotate the plate 180 degrees and continue.

5. When the experiment is finished:

l Save the experiment. Refer to the instructions on page 57 to perform calculationsand determine pass/fail.


l Test descriptions are provided on page 54




The recommended method for testing the instrument’s alignment, repeatability, andaccuracy is to use Absorbance Test Plate BTI #7260522 (see page 52). If the test plate isnot available, however, Liquid Test 2 can be used for these tests.

Materials


l A new 96-well, clear, flat-bottom microplate (Corning Costar #3590 is recommended)

l Ten test tubes, numbered consecutively, set up in a rack

l Calibrated hand pipette (Class A volumetric pipette recommended)

l Solution A or B (see the instructions for Liquid Test 1)

l A 0.05% solution of deionized water and Tween 20

l If using Gen5, the protocol 800 TS Abs Test 2.prt, described on page 56

Test Procedure

1. Create a percentage dilution series, beginning with 100% of the original concentratedstock solution (A or B) in the first tube, 90% of the original solution in the second tube,80% in the third tube, all the way to 10% in the tenth tube. Dilute using the 0.05%solution of deionized water and Tween 20. This solution can also be made by diluting theBioTek wetting agent 200:1.

Tube Number 1 2 3 4 5 6 7 8 9 10

Volume of Original ConcentratedSolution (mL) 20 18 16 14 12 10 8 6 4 2

Volume of 0.05% Tween Solution(mL) 0 2 4 6 8 10 12 14 16 18

Absorbance expected if originalsolution is 2.0 at 200 μL 2.0 1.8 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2



The choice of dilutions and the absorbance of the original solution can be varied. Usethis table as a model for calculating the expected absorbances of a series of dilutions,given a different absorbance of the original solution.

2. Pipette 200 µL of the concentrated solution from Tube 1 into each well of the firstcolumn, A1 to H1, of a new flat-bottom microplate.

3. Pipette 200 µL from each of the remaining tubes into the wells of the correspondingcolumn of the microplate (Tube 2 into wells A2 to H2, Tube 3 into wells A3 to H3, and soon).



4. Read the microplate five times at 450 nm (Primary)/630 nm (Secondary) using theNormal Read Speed.

5. Do not discard the plate. You will use it for the Alignment Test.

6. Print the five sets of raw data or send it to the USB flash drive for use in Excel or otherprogram.

7. Perform the Linearity and Repeatability calculations described in Chapter 5.

8. (Alignment Test) Rotate the plate 180 degrees in the carrier, and then read the platefive more times, with the same protocol as described in step 4. This test results in valuesfor the four corner wells that can be used to determine alignment.

9. Perform the Alignment calculations described in Chapter 5.

If using Gen5:

4. If using Gen5, create an experiment based on the 800 TS Abs Test 2 protocol and readthe plate. When prompted, rotate the plate 180 degrees.

5. When finished:





l Test descriptions are provided on page 54.

Absorbance Liquid Test 3 (optional)

Absorbance Liquid Test 3 is provided for sites requiring proof of linearity at 340 nm.This test is optional because the 800 TS has good "front end" linearity throughout itswavelength range. As an alternative, the 340 nm Absorbance Test Plate (BTI #7260551)may be used for this test.

This test verifies operation of the reader at 340 nm.

Materials


l 340 nm filter installed in the reader

l New 96-well, clear, flat-bottom microplate (Corning Costar #3590 recommended)

l Calibrated hand pipette(s)

l Beakers and graduated cylinder

l Precision balance with readability to 0.01 g

l Buffer solution described below

l If using Gen5, the protocol 800 TS Abs Test 3.prt, described on page 56

Buffer Solution

l Deionized water

l Phosphate-Buffered Saline (PBS), pH 7.2–7.6, Sigma tablets, #P4417 (or equivalent)

l β-NADH Powder (β-Nicotinamide Adenine Dinucleotide, Reduced Form) Sigma bulkcatalog number N 8129, or preweighed 10-mg vials, Sigma number N6785-10VL (orBioTek PN 98233). Store the powder according to the guidelines on its packaging.



1. Prepare a PBS solution from the Sigma tablets.

2. In a beaker, mix 50 mL of the PBS solution with 10 mg of the β-NADH powder and mixthoroughly. This is the 100% Test Solution.

Test Procedure

1. Prepare the 75% Test Solution by mixing 15 mL of the 100% Test Solution with 5 mL ofthe PBS Solution.

2. Prepare the 50% Test Solution by mixing 10 mL of the 100% Test Solution with 10 mL ofthe PBS Solution.

3. Carefully pipette the three solutions into a new 96-well microplate:

l 150 µL of the 100% Test Solution into all wells of columns 1 and 2

l 150 µL of the 75% Test Solution into all wells of columns 3 and 4

l 150 µL of the 50% Test Solution into all wells of column 5 and 6



4. Read the microplate five times at 340 nm using the Normal Read Speed.

5. Print the five sets of raw data or send it to the USB flash drive for use in other program.

6. Perform the Results Analysis calculations described in Chapter 5.

If using Gen5:

4. Create a Gen5 experiment based on the 800 TS Abs Test 3 protocol and read the plate.




Appendix A

Specifications

This appendix contains BioTek's published specifications for the 800 TS.

General Specifications 76

Absorbance Specifications 77

76 | Appendix A: Specifications

General Specifications

Microplates

The 800 TS accommodates standard 6-, 12-, 24-, 48-, 96-, and (with the NB model) 384-well microplateswith 128 x 86mm geometry and, if using Gen5 software with the NB model, 60-, 75-, and 96-wellTerasaki plates and BioCell vessels.

Maximum plate height: 0.90" (22.86mm)

Hardware and Environmental

Light Source800TS/800TSI models: Tungsten gas-filled bulb.800TSUV/800TSUVI/800TSNB models: Halogen bulb

Dimensions 16.5" D x 15" W x 7" H (41.9 x 38.1 x 17.8 cm)

Weight: < 22 lbs (10 kg)

Environment: Operational temperature range: 18° to 40°C (64°F to 104°F)

Humidity: 10% to 85% relative humidity (non-condensing)

Power Supply: 24-volt DC power supply compatible with 100–240 V AC @50–60 Hz

Power Consumption:40Wmaximum, non-incubated models; 150Wmaximum, incubatedmodels

Incubation:

Temperature control for 800TSI model: 6°C over ambient to 50°C.

Temperature control for 800TSUVI model: 8°C over ambient to 50°C

Temperature stability and uniformity is ± 0.5°C across the plate@ 37°C.


Absorbance Specifications | 77

Absorbance Specifications

Standard Models (800TS/800TSI)Wavelength Range: 400 nm to 750 nm

Absorbance Measurement Range

Normal Read Mode: 0.000 to 4.000 OD

Rapid Read Mode: 0.000 to 4.000 OD

Sweep Read Mode: 0.000 to 3.000 OD

Absorbance Resolution

0.001 OD when operated in standalone mode0.0001 OD when operated with Gen5

Accuracy

Normal Read Mode: ±1.0% ±0.010 OD from 0.000 to 2.000 OD@ 405 nm

Rapid Read Mode: ±2.0% ±0.020 OD from 0.000 to 2.000 OD@ 405 nm

Sweep Read Mode: ±1.0% ±0.020 OD from 0.000 to 1.000 OD@ 405 nm

Linearity

Normal Read Mode: ±1.0% ±0.010 OD from 0.000 to 2.000 OD@ 405 nm

±3.0% ±0.010 OD from 2.000 to 3.000 OD @ 450 nm

Rapid Read Mode: ±2.0% ± 0.010 OD from 0.000 to 2.000 OD@ 405 nm

Sweep Read Mode: ± 1.0% ± 0.010 OD from 0.000 to 1.000 OD@ 405 nm

Repeatability (STD)

Normal Read Mode: ± 0.5% ± 0.005 OD from 0.000 to 2.000 OD@ 405 nm

Rapid Read Mode: ± 1.0% ± 0.010 OD from 0.000 to 2.000 OD@ 405 nm

Sweep Read Mode: ± 2.0% ± 0.020 OD from 0.000 to 1.000 OD@ 405 nm

Throughput

From carrier start to carrier stop:



96-Well, SingleWavelength, Normal Read Mode: 39 seconds

96-Well, DualWavelength, Normal Read Mode: 73 seconds

96-Well, SingleWavelength, Rapid Read Mode: 26 seconds

96-Well, SingleWavelength, Sweep Read Mode: 18 seconds

Ultraviolet (UV) Models (800TSUV/800TSUVI)Wavelength Range: 340 nm to 750 nm


Normal and Rapid Read Mode (340 nm–399 nm): 0.000 to 4.000 OD

Normal and Rapid Read Mode (400 nm–750 nm): 0.000 to 4.000 OD

Sweep Read Mode (400 nm–750 nm): 0.000 to 3.000 OD



Accuracy (340 nm to 399 nm)

Normal Read Mode: ±2.0% ± 0.010 OD from 0.000 to 2.000 OD@ 340 nm


Accuracy (400 nm to 750 nm)



Sweep Read Mode: ±1.0% ±0.020 OD from 0.000 to 1.000 OD@ 405 nm

Linearity (340 nm to 399 nm)




Absorbance Specifications | 79

Linearity (400 nm to 750 nm)


±3.0% ± 0.010 OD from 2.000 OD to 3.000 OD@ 450 nm


Sweep Read Mode: ±1.0% ± 0.010 OD from 0.000 to 1.000 OD@ 405 nm

Repeatability (340 nm to 399 nm)



Repeatability (400 nm to 750 nm)



Sweep Read Mode: ±2.0% ± 0.020 OD from 0.000 to 1.000 OD@ 405 nm

Throughput



96-Well, DualWavelength, Normal Read Mode: 73 seconds


96-Well, SingleWavelength, Sweep Read Mode: 18 seconds

Narrow Bean (NB) Models (800TSNB)Wavelength Range: 400 nm to 750 nm


Normal and Rapid Read Mode (96-well): 0.000 to 4.000 OD

Normal and Rapid Read Mode (384-well): 0.000 to 4.000 OD





Accuracy

Normal Read Mode (96-well): ±1.0% ±0.010 OD from 0.000 to 2.000 OD@ 405 nm

Rapid Read Mode (96-well) ±2.0% ±0.020 OD from 0.000 to 2.000 OD@ 405 nm

Normal Read Mode (384-well): ±2.0% ±0.020 OD from 0.000 to 2.000 OD@ 405 nm

Rapid Read Mode (384-well): ±2.5% ±0.020 OD from 0.000 to 2.000 OD@ 405 nm

Linearity

Normal Read Mode (96-well): ±1.0% ± 0.010 OD from 0.000 to 2.000 OD at 405 nm

±3.0% ±0.010 OD from 2.000 to 3.000 OD @ 450 nm

Rapid Read Mode (96-well): ±2.0% ± 0.010 OD from 0.000 to 2.000 OD@ 405 nm

Normal Read Mode (384-well): ±2.5% ± 0.010 OD from 0.000 to 2.000 OD@ 405 nm

Rapid Read Model (384-well): ±2.5% ± 0.010 OD from 0.000 to 2.000 OD@ 405 nm

Repeatability (STD)





Throughput




384-Well, DualWavelength, Normal Read Mode: 3minutes, 40 seconds

384-Well, DualWavelength, Rapid Read Mode: 2minutes


Appendix B

Error Codes

This appendix lists and describes 800 TS error codes that may appear inGen5.

Error Codes Overview 82

Error Codes 82

82 | Appendix B: Error Codes

Error Codes OverviewWhen a problem occurs during operation with the 800 TS, an error code may appear onthe touchscreen or in Gen5. Error codes typically contain four characters, such as “4168,”and in most cases are accompanied by descriptive text. With many errors, the instrumentwill beep repeatedly; press the carrier eject button to stop this alarm.Some problems can be solved easily, whereas others can be solved only by trained BioTekservice personnel. This appendix lists the most common and easily resolved error codesthat you may encounter.

Error codes beginning with “A” (e.g., A100) indicate conditions that require immediateattention. If this type of code appears, turn the instrument off and on. If the system testdoes not conclude successfully, record the error code and contact BioTek’s TechnicalAssistance Center.

If an error code appears in Gen5, you should run a system test for diagnostic purposes. InGen5, select System > Diagnostics > Run System Test. Having the system test reportbefore calling the BioTek Technical Assistance Center can speed the resolution of the error.

If an error message appears while an experiment is in process and afterhaving received measurement data, it is your responsibility todetermine if the data is valid.

Contact Info: BioTek Service/TACUse this appendix to diagnose problems and solve them if possible. If you need furtherassistance, contact BioTek’s Technical Assistance Center.Phone: 800-242-4685 (toll free in the U.S.)802-655-4740 (outside the U.S.)Fax: 802-654-0638E-Mail: [email protected]

Error CodesThis table lists the most common and easily resolved error codes that you may encounter.If an error code appears in Gen5, look for it here. If you find the code, follow thesuggestions provided for solving the problem. If you cannot find the code or if you areunable to solve the problem, please contact BioTek’s Technical Assistance Center. TheGen5 Help system also provides troubleshooting tips.


Error Codes | 83

Code Description and Probable Causes

020x Motor sensor not found. Axis fails to home.

x =motor axis0 = x axis motor1 = y axis motor2 = filter motor

Possible causes:

Dirty axis rail where the bearings are worn and cause too much friction.

Defective or broken optical sensors or connection.

Defectivemotor, motor controller, or connection.

Broken or untensioned drive belt.

Obstruction in read chamber.

Check that the shipping screw has been removed.

Check for motor obstructions.

Run a self-test.

Note: In cases where a sensor is not functioning, themotor may drive the axis toits mechanical stop and generate substantial noise but will not cause permanentdamage.

040x Motor failed positional verify.

Motor failed to reach the same position when moved a known number of stepsfrom the home position and back.


Probable causes:

Dirty linear bearing

Obstruction in read chamber

Incorrect belt tension or loose clamp

Troubleshooting:

Restart the instrument (this will reset the home position).




130x Motor not homed successfully.


Check for motor obstructions.

Run a self-test.

05xy Analog reading saturated.

x = filter positiony = readset

Check filter configuration.

Check for light intrusion.

Run self-test.

060x Air reading too low.

x = filter position

Check lamp.

070x Signal to noise too low.

x = filter position

Check for light infiltration.

Ensure reader is not too close to another instrument that generates EMI (hotplate, freezer, PC monitor)

0800 Analog offset out of range.

Hardware error.

Check for ambient light leaks.

Ensure the power supply is not too close to the instrument.

Call TAC.

0C0x Power supply failure.

x = failed test

Hardware error. Call TAC.


Error Codes | 85


0D0x Attempted read with wavelength that has not been calibrated.

x = readset


Run self-test.

0E0x Attempted read with wavelength that is not present in reader.

x = readset


Run self-test.

0F00 Analog signal error: too low during gain calibration or changed too muchduring read.


Check for light infiltation.

Run self-test.

1100 Configuration checksum error. Configuration data corrupted.

This error indicates that the stored checksum value from the configuration datadoes not match the actual checksum of the current configuration data.

Recreate the assay definition in the correct version of the software and downloadit again.

Call TAC.

1200 Carrier corner calibration mission.

Hardware/software error. Call TAC.

130x Motor not homed successfully.


Check for motor obstruction.

Run self-test.




140x Motor cannot reach expected position.



15xy Incubation error.

x = failure type

y = thermistor bitfield

Run a self-test for more details on failing zone.

1600 Unexpected abort or define assay command checksum failed.

Call TAC.

1700 Invalid kinetic interval.

Check assay.

1900 Memory allocation error.


1A00 Motor position table invalid.


1B00 Analog controller communication error.

Check software versions. Call TAC.

1D00 Filter configuration changed with map off.

Run a self-test.

2100 Invalid command parameter.


2600 Invalid plate dimensions.

Check plate definition.

2700 PC communication error.

Check connection to PC.


Error Codes | 87


280x Motor in use.



2Dxx Assay definition error.

xx = error type


4000 Onboard protocol checksum failed.


4010 Onboard protocol storage full.

Delete protocols to make space.

4020 Onboard protocol duplicate name not allowed.

Change the protocol name to one that is unique.

4030 Onboard protocol name empty.

Change the protocol name.

4040 Onboard protocol blanks defined beyond plate limits.

Verify that the blanks are valid for the plate type.

4050 Absorbance test plate certification past due.

Check test plate certification.




50xx USB errors:

13 = Error writing to USB flash drive

14 = Error reading from USB flash drive

15 = Error creating folder on USB flash drive

16 = Error opening file on USB flash drive

17 = Error closing file on USB flash drive

18 = Error changing folder on USB flash drive

19 = Error writing to USB printer

1A = Error deleting file on USB flash drive

1C = No USB output selected

1D = No USB flash drive detected

Troubleshooting:

Use a different USB flash drive.

Check the printer connection.

Select results output at Instrument > Output.

Insert flash drive.

8000 and up Fatal error. Contact TAC.


Appendix A

Sample Reports

This appendix contains sample System Test and Absorbance Plate Testreports for the 800 TS.

90 | Appendix C: Sample Reports

Sample System Report


Sample Absorbance Test Plate Report | 91

Sample Absorbance Test Plate Report



Sample Assay Results

Sample (Report) CSV File


Sample Assay Results | 93

Sample (Gen5 input) TXT File


absorbance microplate reader

Documents