abstract a biosensor that utilizes bacterial stress responses to oxidative toxins, such as the...
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Predicting the public health impact of oxidative toxins using a bacterial glutathione-gated potassium efflux stress response biosensor Kaoru Ikuma1, Ines D. S. Henriques1, Beverly A. Rzigalinski4, Brian J. Love2, and Nancy G. Love1,3*
Departments of 1Civil & Environmental Engineering, 2Materials Sciences and Engineering, and 3Biological Sciences, Virginia Tech, Blacksburg, VA 240614Edward Via Virginia College of Osteopathic Medicine, Blacksburg, VA 24060*Corresponding AuthorRat brain cell mitochondrial damage assay (MTT assay):Microscale immobilization of P. aeruginosa cells were accomplished in alginate beads with an average diameter of 200 mCells were stressed immediately after immobilization but viability recovered over time3 hours after immobilization10 days after immobilizationFigure 2. LIVE/DEAD staining of immobilized cells. Green dots indicate live cells, red dots indicate dead cells.Figure 1. Comparison of alginate bead sizes to a dime. Microscopic image of alginate microbeads are also shown at 200x resolution.Rat brain cell mitochondrial damage assay (MTT assay):